scholarly journals Pemberian Ekstrak Daun Cincau (Mesona palustris BL) Meningkatkan Kadar Superoksida Dismutase (SOD) Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Karnia Tarnajaya ◽  
Alex Pangkahila ◽  
Wimpie Pangkahila ◽  
Ferbian M. Siswanto
Keyword(s):  
Physical Training ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Male Wistar Rats ◽  
The Mean

Abstract: This study was aimed to prove that administration of black cincau (Mesona palustris BL) leaf extract increased superoxide dismutase (SOD) levels of overtraining-induced male Wistar rats (Rattus norvegicus). This was a true experimental study using the randomized pretest-posttest control group design. Subjects were 16 male rats (Rattus norvegicus), 6-month old, Wistar strain, weighing 180-200 g, SOD level <39.3 U/mL (normal SOD level), divided into two groups: the control group (P0) and the treatment group (P1). The P0 group was given excessive physical training and aquadest for 14 days, while the P1 group was given excessive physical training and black cincau leaf extract of 54 mg/200 g BW daily for 14 days. The results showed that the mean SOD level before treatment (pretest) in the P0 group was 30.63±2.26 U/mL while in the P1 group was 31.31±2.52 U/mL (P >0.05). After 14-day treatment (posttest), the mean SOD level in the P0 group was 29.36±2.83 U/mL while in the P1 group was 41.31±2.35 U/mL (P <0.01). Further analysis showed that there was no change of SOD levels in the P0 group (P > 0.05), but in the P1 group there was a significant increase of SOD levels from 31.31±2.52U/mL to 41.31±2.35U/mL (P <0.01). Conclusion: Administration of black cincau leaf extract of 54 mg per 200 g of body weight increased SOD level of overtraining-induced male Wistar rats (Rattus norvegicus).Keywords: black cincau leaves, SOD, excessive physical activityAbstrak: Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun cincau (Mesona palustris BL) dapat meningkatkan kadar superoksida dismutase (SOD) tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih. Jenis penelitian ialah eksperimental murni menggunakan randomized pretest-posttest control group design. Subjek penelitian ialah 16 ekor tikus putih jantan (Rattus norvegicus) galur Wistar berumur 6 bulan, sehat, berat badan 180-200 gr, dan kadar SOD <39,3 U/mL (kadar SOD normal), yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (P0) dan kelompok perlakuan (P1), masing-masing berjumlah 8 ekor tikus. Kelompok P0 diberikan pelatihan fisik berlebih dan akuades secara sonde selama 14 hari, dan kelompok P1 diberikan pelatihan fisik berlebih dan ekstrak daun cincau dosis 54 mg/200 gr BB tikus selama 14 hari. Hasil penelitian menunjukkan bahwa rerata kadar SOD sebelum perlakuan (pretest) pada kelompok P0 ialah 30,63±2,26 U/mL, sedangkan pada kelompok P1 ialah 31,31±2,52 U/mL (P >0,05). Setelah perlakuan selama 14 hari (posttest), rerata kadar SOD pada kelompok P0 ialah 29,36±2,83 U/mL, dan pada kelompok P1 ialah 41,31±2,35 U/mL (P <0,01). Hasil analisis efek perlakuan menunjukkan bahwa pada kelompok P0 tidak terjadi perubahan kadar SOD (P >0,05), namun pada kelompok P1 terjadi peningkatan bermakna kadar SOD dari 31,31±2,52 U/mL menjadi 41,31±2,35U/mL (P <0,01). Simpulan: Ekstrak daun cincau dosis 54 mg/200 gr BB tikus dapat meningkatkan kadar SOD tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih.Kata kunci: daun cincau, SOD, aktivitas fisik berlebih

scholarly journals Pemberian Ekstrak Daun Gandarusa (Justicia gendarussa Burm. f.) Menurunkan Kadar F2-isoprostan Urin Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
DG Ika Akpriyanti ◽  
Wimpie Pangkahila ◽  
IGM Aman
Keyword(s):  
Treatment Group ◽  
Physical Training ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Oxygen Species ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

Abstract: Excessive physical activity increases the consumption of oxygen which can result in increased levels of reactive oxygen species (ROS) causing oxidative stress characterized by the elevation of F2-isoprostane as a byproduct of lipid peroxidation. Willow (Justicia gendarussa Burm. f.) has an antioxidant activity that is able to inhibit and break the chain reaction of free radicals and prevent lipid peroxidation. This study was aimed to prove that willow leaf extract could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training. This was a true experimental study using 16 male Wistar rats (Rattus norvegicus), aged 2-3 months, weighing 180-200 g as subjects. The control group (P0) was treated with a placebo and excessive physical training, and the treatment group (P1) was treated with the willow leaf extract of 200mg/rat/day and excessive physical training. Before and after 14 days of treatment, the urine samples were collected and their levels of F2-isoprostane 8-isoPGF2α were examined by using enzyme immunoassay kit (EIA). The results showed that the mean level of F2-isoprostane in the control group (P0) before treatment (pretest) was 5.20±0.483 ng / mL while in the treatment group (P1) was 5.46±0.655 ng / mL (P > 0.05). On the other hand, after treatment for 7 days (post-test), the mean level of F2-isoprostane in the control group (P0) was 5.34±0.476 ng/mL and in the treatment group (P1) was 3.61±0.389 ng/mL (P < 0.01). Conclusion: The extract of willow (Justicia gendarussa Burm. f.) leaf could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training.Keywords: willow leaf, F2-isoprostane, excessive physical trainingAbstrak: Aktivitas fisik berlebih akan meningkatkan komsumsi oksigen yang dapat mengakibatkan peningkatan kadar reactive oxygen species (ROS) sehingga menyebabkan stres oksidatif yang ditandai dengan meningkatnya F2-isoprostan sebagai salah satu produk peroksidasi lipid. Tanaman gandarusa (Justicia gendarussa Burm. f.) mengandung antioksidan yang dapat mencegah reaksi peroksidasi lipid. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun gandarusa dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih. Jenis penelitian ialah eksperimental dengan subjek 16 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 6 bulan, berat badan 180-200 gr, dengan kadar F2-isoprostan >2ng/mL. Kelompok kontrol (P0) diberikan pelatihan fisik berlebih dan plasebo aquadest 2ml sedangkan kelompok perlakuan (P1) diberikan pelatihan fisik berlebih dan ekstrak daun gandarusa dosis 200mg/ekor/hari. Sebelum dan setelah 14 hari perlakuan, sampel urin ditampung dan diperiksa kadar F2-isoprostan menggunakan 8-isoPGF2α enzyme immunoassay kit (EIA) dari assay design untuk data pretest dan post-test. Hasil penelitian menunjukkan rerata kadar F2-isoprostan pada kelompok P0 sebelum perlakuan (pretest) ialah 5,20±0,483 ng/mL, sedangkan pada kelompokP1 ialah 5,46±0,655 ng/mL (P > 0,05). Setelah perlakuan selama 14 hari (post-test), rerata kadar F2-isoprostan pada kelompok P0 ialah 5,34±0,476 ng/mL dan pada kelompok P1 ialah 3,61±0,389 ng/mL (P < 0,01). Simpulan: Ekstrak daun gandarusa (Justicia gendarussa burm. f.) dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih.Kata kunci: daun gandarusa, F2-isoprostan, pelatihan fisik berlebih

scholarly journals THE EFFECT OF SEMBUNG LEAF EXTRACT (BLUMEA BALSAMIFERA) ON THE NUMBER AND DIAMETER OF RATS LEYDIG CELLS INDUCED BY HIGH-FAT DIET

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gede Widhiantara ◽  
Anak Agung Ayu Putri Permatasari ◽  
Putu Angga Wiradana
Keyword(s):  
Treatment Group ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Control Group Design ◽  
Core Diameter ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.

scholarly journals Pemberian suplemen Swissoats A3® meningkatkan kadar hormon estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Trisna A. Widhayanthi ◽  
Wimpie Pangkahila ◽  
Gde Ngurah Indraguna Pinatih
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Indirect Elisa ◽  
Control Group ◽  
Medial Canthus ◽  
Control Group Design ◽  
Group Design ◽  
Estrogen Level ◽  
Female Wistar Rats ◽  
The Mean

Abstract: Aging is a physiological process that occurs in all living creatures. Swissoats A3® is an anti-aging supplement that contains phytoestrogens. This study was aimed to prove that Swiss oats A3® supplementation could increase estrogen levels in menopausal female Wistar rats (Rattus norvegicus). This was an experimental study with a completely randomized pretest-posttest control group design. Subjects were 20 menopausal female rats (Rattus norvegicus), Wistar strain, healthy, aged 15-16 months, weighing 200 g, divided into 2 groups: the control group (P0), treated with distilled water for 21 days and the treatment group (P1), treated with Swissoats A3® of 34.2 mg/rat/day for 21 days. Before and after 21 days of treatment, 1 ml venous blood was drawn through the medial canthus of orbital sinus using microcapillary tube and the estrogen levels were meassured by using indirect ELISA method. The results showed that before treatment, the mean estrogen level in P0 group was 14.96±2.0468 pg/ml and in P1 group was 14.21±2.3905 pg/ml (P > 0.05). Meanwhile, after 21-day treatment, the mean estrogen level in P0 group was 15.07±1.8851 pg/ml, whereas in P1 group was 28.33±3.2052 pg/ml (P < 0.01). The analysis showed that the Swissoats A3® could elevate the estrogen levels significantly (P < 0.01) in group P1 (2.3905±14.21 vs 28.33±3.205). Conclusion: Swissoats A3® supplementation could increase the estrogen levels in menopausal female Wistar rats (Rattus norvegicus).Keywords: Swiss oats A3®, estrogen, female Wistar rats, menopauseAbstrak: Penuaan (aging) merupakan suatu proses fisiologik yang dialami oleh seluruh mahluk hidup. Swissoats A3® merupakan suplemen anti-aging yang mengandung fitoestrogen. Penelitian ini bertujuan untuk membuktikan bahwa pemberian suplemen Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar. Jenis penelitian ialah eksperimental dengan randomized pretest-posttest control group design. Subjek penelitian ialah 20 ekor tikus putih betina (Rattus norvegicus) galur Wistar, sehat, menopause, umur 15-16 bulan, berat badan 200 gr. Tikus dibagi menjadi 2 kelompok yaitu kelompok kontrol (P0) yang diberikan aquadest secara sonde selama 21 hari dan kelompok perlakuan yang diberikan suplemen Swissoats A3® dosis 34,2mg/ekor/hari secara sonde selama 21 hari. Sebelum dan sesudah 21 hari perlakuan, darah vena diambil melalui medial canthus sinus orbitalis sekitar 1 ml dengan menggunakan tabung mikrokapiler dan dilakukan pemeriksaan kadar estrogen menggunakan metode indirect ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan, rerata kadar estrogen kelompok P0 14,96±2,0468 pg/ml dan kelompok P1 14,21±2,3905 pg/ml (P > 0,05). Setelah perlakuan selama 21 hari, rerata kadar estrogen kelompok P0 15,07±1,8851 pg/ml dan kelompok P1 28,33±3,2052 pg/ml (P < 0,01). Analisis efek perlakuan menunjukkan terjadinya peningkatan kadar hormon estrogen yang sangat bermakna (P < 0,01) pada kelompok P1 (14,21±2,3905 vs 28,33±3,205). Simpulan: Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur wistar.Kata kunci: Swissoats A3®, estrogen, tikus wistar betina, menopause

scholarly journals Pelatihan Fisik Berlebih Menurunkan Jumlah Hematopoietic Stem Cells (HSCs) Dibandingkan Pelatihan Fisik Seimbang pada Tikus (Rattus norvegicus) Wistar Jantan

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Zenitalia . ◽  
Alex Pangkahila ◽  
Wimpie Pangkahila ◽  
Ferbian M. Siswanto
Keyword(s):  
Stem Cells ◽  
Physical Exercise ◽  
Hematopoietic Stem Cells ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Hematopoietic Stem ◽  
Group Design ◽  
Male Wistar Rats

Abstract: This study was aimed to prove that balanced physical exercise increased mobilization of hematopoietic stem cells (HSCs) compared to excessive physical exercise in male Wistar rats (Rattus norvegicus). This was an experimental study with the randomized pretest-posttest control group design. Subjects were 24 male Wistar rats (Rattus norvegicus), 2.5-3 months old, weighing 180-200 g, healthy (active and did not show any anatomical abnormality), divided into two groups of 12 rats each. One group (P0) was treated with balanced physical exercise and the other group (P1) was treated with excessive physical exercise. Before (pretest) and 4 weeks after treatment (posttest), peripheral blood of 1 ml was drawn through medial canthus sinus orbitalis for examination of HSCs number in peripheral blood quantitatively. The results showed that the number of HSCs in the P0 group was increased from 1.60±0.70 x 106 cell/μl before treatment to 2.70±0.62 x 106 cells/μl after 4-week treatment (P <0.05). Meanwhile, in the P1 group, the number of HSCs was decreased from 1.74±0.68 x 106 cells/μl before treatment to 1.34±0.55 x 106 cells/μl after 4-week treatment (P <0.05). Conclusion: Excessive physical exercise decreased number of hematopoietic stem cells (HSCs) compared to balanced physical exercise in male Wistar rats (Rattus norvegicus).Keywords: balanced physical exercise, excessive physical exercise, hematopoietic stem cells (HSCs)Abstrak: Tujuan penelitian ini ialah untuk membuktikan bahwa pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan. Jenis penelitian ini ialah eksperimental dengan randomized pre-post test control group design. Subyek penelitian ialah 24 ekor tikus Wistar (Rattus norvegicus) jantan, umur 2,5-3 bulan, berat badan 180-200 gr, sehat (aktif dan tidak menunjukkan abnormalitas anatomi) yang dibagi menjadi dua kelompok yaitu P0 dan P1, masing-masing berjumlah 12 ekor. Kelompok P0 diberikan pelatihan seimbang dan kelompok P1 diberi pelatihan fisik berlebih. Sebelum (pretest) dan 4 minggu setelah perlakuan (posttest), darah diambil sebanyak 1 ml melalui medial canthus sinus orbitalis untuk pemeriksaan kadar HSCs darah tepi secara kuantitatif. Hasil penelitian menunjukkan bahwa pada kelompok P0 terjadi peningkatan jumlah HSCs dari 1,60±0,70 x106 sel/μl pretest menjadi 2,70±0,62 x106 sel/μl posttest (P <0,05). Pada kelompok P1 terjadi penurunan jumlah HSCs dari 1,74±0,68 x106 sel/μl pretest menjadi 1,34±0,55 x106 sel/μl posttest (P <0,05). Simpulan: Pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan.Kata kunci: pelatihan fisik seimbang, pelatihan fisik berlebih, hematopoietic stem cells (HSCs)

scholarly journals Pemberian resveratrol oral mencegah peningkatan F2-Isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang dipapar tartrazine

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Yulyani . ◽  
Wimpie Pangkahila ◽  
Ferbian M. Siswanto
Keyword(s):  
Oral Administration ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Before And After ◽  
Male Wistar Rats ◽  
Immuno Assay ◽  
Enzyme Immuno Assay

Abstract: This study was aimed to prove that oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus). This was an experimental study using the pretest-posttest control group design. Subjects were 24 rats (Rattus norvegicus), Wistar strain, healthy, 2-3 months old, weighing 200-220 g, divided into 2 groups with 12 rats each. The first group (P0), the control group, was given a placebo of 2 ml distilled water 2 hours prior to the administration of tartrazine 75 mg/kg body weight. The second group (P1), the treatment group, was given resveratrol of 20 mg/kg 2 hour prior to the administration of tartrazine 75 mg/kg. Rats’ urine was collected before and after treatment for 4 weeks. Level of F2-isoprostane was examined by using an 8-iso-PGF2α enzyme immuno assay kit. The comparative analysis of the pretest groups showed that there was no difference between the average levels of F2-isoprostane in both groups (5.45±0.62 ng/mL in P0 group vs 5.42±0.64 ng/mL in P1 group) (P > 0.05). Meanwhile, after treatment for 4 weeks, the average level of F2-isoprostane in the P0 group was significantly different from the P1 group (6.61±0.93 ng/mL vs 3.79±0.48 ng/mL) (P < 0.01). Analysis of the treatment effect showed a significant increase of F2-isoprostane level in the P0 group, and a significant decrease in the P1 group (P < 0.01). Conclusion: Oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus).Keywords: tartrazine, resveratrol, F2-isoprostaneAbstrak: Tujuan penelitian untuk membuktikan pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan dalam urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine. Jenis penelitian eksperimental dengan menggunakan pretest-posttest control group design. Subyek penelitian ialah 24 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 2-3 bulan, dengan berat badan 200-220 gr, dibagi menjadi 2 kelompok, masing-masing berjumlah 12 ekor tikus. Kelompok pertama (P0) ialah kelompok kontrol, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan plasebo berupa aquadest 2 ml. Kelompok kedua (P1) ialah kelompok perlakuan, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan resveratrol 20 mg/kg BB. Saat sebelum dan sesudah perlakuan selama 4 minggu, urin tikus dikoleksi untuk pemeriksaan kadar F2-isoprostan menggunakan 8-iso-PGF2α enzyme immuno assay kit. Analisis komparasi sebelum perlakuan (pretest) menunjukkan rerata kadar F2-isoprostan pada kedua kelompok tidak berbeda nyata (5,45±0,62 ng/mL vs 5,42±0,64 ng/mL) (P > 0,05). Setelah perlakuan selama 4 minggu, rerata kadar F2-isoprostan pada kelompok P0 berbeda nyata dibandingkan kelompok P1 (6,61±0,93 ng/mL vs 3,79±0,48 ng/mL) (P < 0,01). Analisis efek perlakuan menunjukkan terjadi peningkatan kadar F2-isoprostan pada kelompok P0 dan penurunan bermakna pada kelompok P1 (P < 0,01). Simpulan: Pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine.Kata kunci: tartrazine, resveratrol, F2-isoprostan, urin

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Unsaturated Fat ◽  
Post Test ◽  
Channa Striata ◽  
The Mean

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Wilcoxon Test ◽  
Control Group ◽  
Increase Body Weight ◽  
Control Group Design ◽  
Tissue Mass ◽  
Group Design ◽  
Post Test ◽  
Male Wistar Rats

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

scholarly journals The effect of black cumin oil (Nigella sativa) on the reproduction of male Wistar rats

2021 ◽  
Vol 1 (2) ◽  
pp. 45-49
Author(s):  
Syahran Wael ◽  
Didik Wahyudi ◽  
Tayeb Wael ◽  
Zaid Mohamed Jaber
Keyword(s):  
Wistar Rats ◽  
Nigella Sativa ◽  
Cellular Damage ◽  
Control Group ◽  
Control Group Design ◽  
Black Cumin ◽  
Group Design ◽  
Nigella Sativa Oil ◽  
Post Test ◽  
Male Wistar Rats

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33±13.292) compare in the treatment 250 mg/kgbw (40.67±17.512) and 150 mg/kgbw (30.67±8.165). avarage value of count sperm in the control (130.83±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67±23.480) compare in the treatment 250 mg/kgbw (187.50±74.538) and 150 mg/kgbw (140.83±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.

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