scholarly journals PROPHYLACTIC IMMUNODEFICIENCY OF DOGS COMPLEX BIOLOGICAL PREPARATION

Author(s):  
В.Г. СЕМЕНОВ ◽  
А.А. ИШТУДОВ ◽  
Д.А. НИКИТИН ◽  
В.А. АЛЕКСЕЕВ ◽  
А.С. ТИХОНОВ

Цель работы – изучение морфофизиологического профиля и неспецифических защитных сил организма служебных собак в условиях кинологического центра на фоне профилактики иммунодефицита биопрепаратами Dоg-Stim-N-B и Prevention-N-Е. Методология. Исследовательская работа проведена в условиях центра кинологической службы МВД и ветеринарной клиники «Айболит» г. Чебоксары на протяжении 2016-2020 гг. Для проведения научно-исследовательской работы подобрали три группы собак одной породы двухмесячного возраста по 5 особей в каждой (контрольная, 1-я и 2-я опытные). С целью профилактики иммунодефицита организма собакам опытных групп выполнили двукратное внутримышечное инъецирование биопрепаратов Prevention-N-Е (1-я опытная) и Dоg-Stim-N-B (2-я опытная) в дозе 0,1 мл на 1 кг живой массы. Результаты. Двукратная внутримышечная инъекция служебным собакам 1-й и 2-й опытных групп биопрепаратов Dоg-Stim-N-B и Prevention-N-Е на 61-63 и 68-69 сутки после рождения повышала концентрацию гемоглобина в крови по сравнению с контролем на 2,0-5,0 и 4,0-15,0 г/л, количество красных кровяных телец – на 1,09 и 1,10×1012/л, уровень общего белка – на 2,8-5,4 и 2,8-8,2 г/л, в том числе альбуминов – на 2,5-3,4 и 3,3-4,3 г/л, а γ-глобулинов – на 0,8-2,4 и 0,3-2,9 г/л. Кроме того, биопрепараты способствовали активизации клеточного и гуморального звеньев неспецифической устойчивости организма. Заключение. Разработанные биопрепараты Dоg-Stim-N-B и Prevention-N-Е стимулируют гемопоэз, развивают физиологическую эозинофилию, нейтрофилопению со сдвигом ядра вправо и лимфоцитоз, активизируют обмен белка, синтез альбуминов и γ-глобулинов, повышают активность клеточных и гуморальных факторов неспецифической резистентности организма. The purpose of the work – study of morphophysiological profle and non-specifc protective forces of the body of service dogs in the conditions of a canine center against the background of prevention of immunodefciency with biological preparation Dog-Stim-N-B and Prevention-N-E. Methodology. Research work was carried out in the conditions of the center of the canine service of the Ministry of Internal Afairs and the Aibolit veterinary clinic in Cheboksary during 2016-2020. For research work, 3 groups of dogs of one breed were formed at the age of 2 months, 5 heads in each group. In order to prevent immunodefciency of the body, dogs of the 1st experimental group were injected intramuscularly with the biopreparation Prevention-N-E at a dose of 0.1 ml/kg of live weight twice on the 61-63 and 68-69 day after birth, dogs of the 2nd experimental group at the same dose and at the same time injected with the biopreparation Dog-Stim-N-B. Results. Two-fold intramuscular injection to service dogs of the 1st and 2nd experimental groups of DogStim-N-B and Prevention-N-E biologics on the 61-63 and 68-69 day after birth increased blood hemoglobin concentration compared to 2 control.0-5.0 and 4.0-15.0 g/l, number of red blood cells - by 1.09 and 1,10 × 1012/l, the concentration of total protein - on 2.8-5.4 and 2.8-8.2 g/l, including the albumin fraction - on 2,5-3.4 and 3.3-4.3 g/l a γ-globulin - by 0.8-2.4 and 0.3-2.9 g/l. In addition, biologics contributed to an increase in plasma lysozyme activity and bactericidal activity of dog blood serum, which indicates an activation of the humoral link of the body's nonspecifc resistance. Conclusion. Biopreparation Dog-Stim-N-B and Prevention-N-E activate hematopoiesis, cause physiological eosinophilia, neutrophylopenia with neutrophilic nucleus shift to the right and lymphocytosis, stimulate protein exchange, synthesis of albumins and γ-globulins, increases cellular and humoral factors of non-specifc resistance

Author(s):  
N. V. Chorniy ◽  
O. S. Machula ◽  
V. V. Voronyak ◽  
V. P. Lyasota ◽  
O. P. Reshetnichenko

The aim of the work was to study the influence of stimulant drugs – the regenerating biological stimulant (RBS) and Imunolac on the immunological state and productive qualities of piglets. RBS is a complex of organic compounds of animal tissue that stimulates nonspecific immunity and increases the protective functions of the body. Imunolac is a preparation of enzymatic hydrolysis of the cell wall of Lactobacillus, activating the cellular and humoral factors of non-specific animal resistance. To achieve this goal, the following tasks were set: to find out the microclimate condition and sanitary regime in the boxes in which the experimental animals were kept; – to study the influence of immunostimulating drugs on the growth and intensity of the test pigs, their safety; to study the dynamics of immunological parameters (circulating immune complex (CIC), T-and B-lymphocytes), the content of immunoglobulins (Jg G, Jg M and Jg A) while using RBS and Imunolac. The research was carried out in the pedigree plant «Stepnoy» of the Zaporozhye region on pigs of the Large white breed × Landras. Piglets of the control group were injected with 0.9% sodium chloride solution at a dose of 1 ml/head. During the experiment, the microclimate was monitored for temperature, relative humidity, air speed, carbon dioxide, ammonia, air contamination with microflora. Cellular indices were determined from the ratio of neutrophils to E. coli according to VG Gostivu, 1956, bactericidal activity of blood serum (BASK) – according to the method of O.V. Smirnova and TA. Kuzmenoy, 1966, lysozyme activity of blood serum (LASK) according to V. Dorofeychuk, 1968. The content of T lymphocytes was determined according to M. Jondal, 1973, B-lymphocytes – according to N.S. When piglets were grown at the temperature lower than 3,5–8 °C, humidity – by 5–8% higher, the parameters of NH3 and CO2 higher by 0.8–1.2%, the contamination microflora was twice as much as recommended by the sanitary norms the genetic productive potential of the animals is not fully realized, while – the highest daily average weight gain was in the pigs, which were injected intramuscularly with RBS growth stimulant. They exceeded the animals from the control group on the 20th and 30th days of the experiment – by 24.3% and 14.4%, live weight – by 15.3% (P < 0.05). The growth rate in the animals that were injected with imunolac was less expressed; the diseases with the symptoms of dyspepsia and bronchopneumonia were registered in 5–10% of the pigs from the experimental groups that received RBS and imunolac that is 10–12 times less as compared to the control group; in the pigs from the experimental group 1, the BASK was at the level of the average values of 56.52 ± 2.03%, from the experimental group 2 – 59.48 ± 1.80%, by 5.2% higher (P < 0.05); LASK indices in the animals of the experimental group tended to increase but the differences were not trustworthy (P < 0.5); the cell indices of resistance in the pigs that received RBS were higher: the phagocytic activity of blood serum – by 4.3% (P < 0.05), phagocytic number – by 9.7% (P <0.05); the highest concentration of immunoglobulin of class Jg G (21.82 ± 0.3 mg/ml) was revealed in the animals that received RBS preparations, the concentration of the above immunoglobulins was slightly below – 19.86 ± 0.18 mg/ml in the pigs that received imunolac (0–1). For immunoglobulins of class Jg M and Jg A, the fluctuations  between the experimental groups were within the limits of 2.23 ± 0.01 and 2.36 ± 0.09 mg/ml.


2021 ◽  
Vol 935 (1) ◽  
pp. 012043
Author(s):  
V G Semenov ◽  
V V Boronin ◽  
N I Kosyaev ◽  
S S Kozak ◽  
N G Ivanov ◽  
...  

Abstract The article presents the results of studies of the effectiveness of the use of the complex probiotic preparation Immunoflor in young hens of egg cross. In the course of the research work, it was found that the use of the probiotic preparation Immunoflor does not affect the clinical and physiological state of the body of young chickens, but at the same time reduces the incidence, mortality and increases the safety of the chickens by enriching and balancing the poultry diet, reducing the conversion feed, optimization of digestion, stimulation of the development of positive microflora in the gastrointestinal tract. The use of the complex probiotic preparation Immunoflor in the diet of young chickens at a dose of 15 g/t of water and 15 g/t of feed contributes to an increase in the number of erythrocytes, leukocytes and hemoglobin concentration, activation of cellular and humoral factors of nonspecific resistance of the body of chickens, providing a normal physiological state and homeostasis.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.


Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


2022 ◽  
Vol 2022 ◽  
pp. 1-13
Author(s):  
Qiao Chen ◽  
Shihong Liu

Sports can cause the consumption of energy materials in the body. The rational use of nutritional supplements can maintain the homeostasis of the organism, which plays a very important role in improving the competitive performance of sports athletes. The purpose of this study is to explore the effect of nutritional supplements on basketball sports fatigue. The method of this study is as follows: first of all, 15 basketball players in our city were selected as the experimental objects, and they were randomly divided into the experimental group and the control group. The members of the experimental group took nutrients. After the training, 6 days a week, 3 hours in the morning and 3 hours in the afternoon, and the rest was adjusted on Sunday. Before training, four weeks and eight weeks of training, the blood routine indexes and body functions of athletes were tested. The results showed that the number of red blood cells, hemoglobin concentration, and average hemoglobin concentration of ligustilide supplement of the athletes were at the level of 0.05 after 4 weeks and 8 weeks, and the difference was significant ( P < 0.05 ). The nutritional supplements were used in sprint (3.4 s less), long-distance running (12.8 s less), and weight lifting (6.2 kg more) to a certain extent. Nutritional supplements are used as an auxiliary means of diet to supplement the amino acids, trace elements, vitamins, minerals, etc. required by the human body. The conclusion is that nutrition supplement can effectively improve the indexes of athletes’ body in about four weeks, but the effect is not obvious after a long time. This study provides a certain method for the research of nutritional supplements in the field of sports.


Author(s):  
L.G. Kashirina ◽  
◽  
K.I. Romanov ◽  

The antioxidant drug "E-selenium" was used to reduce the processes of LPO in the body of fresh cows. Two groups were formed from the analogous cows of the black-and-white breed: The Control was intact, Experienced received the antioxidant drug injection at a dose of 10 ml at the rate of 1 ml per 50 kg of live weight per head for 4 months of lactation from the second. The diets of animals of all groups were the same, balanced in nutrients and met the standards of the RAAS. The duration of the experiment was 150 days. During the period from the 3rd to the 5th month of lactation, there was a positive dynamic in increasing the number of fat globules in the milk of cows of the Experimental group. By the end of the 4th month, it was higher than the Control by 8.9 %. This confirms the active effect of the drug "E-selenium", which contributes to a positive dynamic in increasing the size of fat globules. Non-enzymatic antioxidant tocopherol is an active element of the anti-oxidative system. Selenium stimu-lates the body's own antioxidant system. In both experimental groups, the relationship between the concentration of peroxidation products and the quantitative composition of fat globules and their size was established. In the milk of cows of the Experimental group, the oxidation processes were inhibited by neutralizing the products (POL), increasing the number of large fat globules and the total fat content in the milk.


Author(s):  
A.A. Ishtudov ◽  
◽  
V.G. Semenov ◽  
D.A. Nikitin ◽  
E.N. Ivanova ◽  
...  

A biopreparation Dog-Stim-N-B was developed and a scientific and practical justification was given for its feasibility in the canine center for raising dogs of the German shepherd breed. Se-lective mobilization of morphological and biochemical profiles of blood, cellular and humoral factors of nonspecific resistance of dog organism is established against the background of intramuscular injection of first-tested biopreparation Dog-Stim-N-B and previously tested Prevention-N-E. The biologics used in the experiments showed a wide range of bio-effects: activated the production of red blood cells and increased the concentration of hemoglobin in the blood of dogs, that is, im-proved hematopoiesis, but did not have a stimulating effect on the production of white blood cells; caused physiological eosinophilia, moderate neutrophylopenia with neutrophilic nucleus shift to the right and lymphocytosis; increased protein metabolism, mainly due to synthesis of albumin and γ-globulin fractions; activated cellular and humoral factors of nonspecific resistance of the organism.


Author(s):  
В. П. Бердник ◽  
О. О. Бублик ◽  
Т. М. Марченко ◽  
В. І. Щербак ◽  
О. Г. Трирог

Наведені результати випробування на 537 курчатах-бройлерах (635 контрольних) в умовах господарства препарату, виготовленого на основі розчину  полтавського бішофіту. У  курчат, яким давали препарат внутрішньо з водою,  порівняно з контролем, була більшою середня жива масса тіла у  43-добовому віці на 50 г, 73-добовому  – на 128 г і 111-добовому – на  103 г. До 43-добового віку загинуло із розрахунку від  початкової загальної кількості 16 (2,9 %) курчат-бройлерів  у дослідній групі і 30 (4,72 %) у контрольній. На контрольних  курчатах-бройлерах, порівняно із дослідними, мали економію на препараті, який не застосовували, і меншій кількості  використаних  кормів, але одержано значно більше збитків   через нижчі прирости живої маси тіла та більшу кількість загиблих. Економічна  вигода  від застосування препарату лише  до 43-добового віку курчат становить 5,8 гривень на  вкладену 1 гривню. Given results of test on 537 chickens-broilers (635 of control) in terms of economy drug produced on the basis of the solution of Poltava bishofit (SPB). Drug was given chickens-broilers of 5-day age group method internally with water in 3 cycles of 24-hour intervals over 7 times in each cycle and 7-day interval between cycles. The chicks, which were given the drug, compared with the control, had a greater average live weight of the body in 43-day age of 50 g, 73-day – 128 g and 111-day – 103 g it shows its positive effect on organisms of chicks up to 2,5 months (time of observations) after the last application in 38-th days of their lives. The drugs based on the SPB will have greater efficiency in the application of the repair chicks and adult hens, i.e. those who live more than 2,5 months. The 43-day age 16 broiler died of the calculation of the initial total number (2,9 %) in experimental group and 30 (4,72 %) in the control. On control broilers, compared with research, savings on the drug, which is not used, and smaller amounts of used feed, but we received much more losses due to lower increases in live body weight and more fatalities. The total value of the economic benefits of the drug only to 43-day age of chicks is 5,8 UAH on each invested 1 UAH.


2021 ◽  
Vol 209 (06) ◽  
pp. 61-70
Author(s):  
Larisa Pashkova

Abstract. The purpose of the research was to develop a method for increasing the meat productivity of sheep under various housing systems. Methods. All studies were conducted according to generally accepted methods. The experimental population of rams of the control and experimental groups was kept until the age of 3 months together with ewes on the pasture (suckling period) with further weaning at the age of 3 months. Further, the rams were divided according to the technologies of keeping: the control group – pasture (graziery), and the experimental group – indoor maintenance (fattening) up to 4 months of age, followed by control slaughter. Monthly weighing of the experimental young rams livestock was carried out. Results. The research work showed that the use of this technological method significantly contributed in increasing average gain and live weight, respectively, by 6.6 % (20 g) and 5.7 % (2.3 kg) and as a result, slaughter weight – by 9.2 % (1.6 kg) in young rams from the experimental group in comparison with analogues of the control group. Chemical, amino acid and microstructural analyses of samples of the longissimus of the livestock showed that the specimens of the young rams of the experimental group had the best food and market advantages. The efficiency of using this technological method is also confirmed by the calculated economic indicators – the difference in the level of profitability was 7.9 % in favor of the rams of the experimental group. The scientific novelty of the research work is a detailed study of the indicators of meat productivity under the influence of this technological method with the justification of its application and the introduction of both scientific and practical contributions to the development of sheep farming.


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