scholarly journals Analysis of the Effect of Body Lossion of Rattus Norvegicus on Granulation Formulation of Chitosan Waste Shell of Placuna Placenta

2021 ◽  
Vol 2 (4) ◽  
pp. 419-424
Author(s):  
Ariyanti Ariyanti ◽  
Eni Masruriati ◽  
Ade Novitasari ◽  
Suzana Wulandari
Keyword(s):  
Weight Loss ◽  
Rattus Norvegicus ◽  
Statistical Tests ◽  
Positive Control ◽  
Negative Control ◽  
Optimal Concentration ◽  
Control Group ◽  
Control Group Design ◽  
Wistar Strain ◽  
Granule Formulation

Weight loss Rattus norvegicus wistar strain can be done by a variety of chemical and traditional medicine. One of the traditional medicines used for weight loss is to use Placuna Placenta chitosan. The purpose of this study was to determine the optimal concentration of Placuna Placuna Placenta granules as a weight loss agent for Rattus norvegicus. This study was an experimental study with 5 concentrations of 5%, 7.5% and 10% Placuna Placuna Placenta chitosan observed for 30 days. The positive control used weight loss drugs in the market, while the negative control used granule formulation without the addition of Placuna Placuna Placenta chitosan. The experimental research design posttest only control group design, treatment or intervention in the experimental group by comparing groups. Comparisons or differences were analyzed statistical tests using the ANOVA test. The optimal concentration of chitosan granule formulation of Placuna Placuna Placenta as a weight loss for Rattus norvegicus wistar strain was 10%.

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

scholarly journals Uji Efektivitas Ekstrak Kulit Apel (Malus sylvestris-mill) Var. Rome Beauty Terhadap Kadar Enzim SGPT Tikus (Rattus norvegicus) Galur Wistar Yang Diinduksi CCL4 (Karbon Tetra Klorida)

2018 ◽  
Vol 11 (2) ◽  
Author(s):  
Hany Yus ◽  
Vina Devi Octaviany ◽  
Kristina Simanjuntak
Keyword(s):  
Liver Damage ◽  
Rattus Norvegicus ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Wistar Strain ◽  
Natural Treatment ◽  
Apple Peel ◽  
Peel Extract

Liver is the largest organ in the human body and often injury due to the way of all the materials into the body through the gastrointestinal tract. CCl4 constitute a chemical compound that can induce liver damage. The natural treatment to reduce liver damage can use natural substance that contains antioxidant one of them is apple. When eating an apple people’s habit throw away the peel even though the peel contain high antioxidants. This present study was conducted to determine the effectiveness of apple peel extract var. rome beauty against to level   of rat (Rattus norvegicus) SGPT that induced by CCl4. This study used 25 rats (Rattus norvegicus) Wistar strain that were divided into 5 groups randomly. These group are positive control, negative control, apple peel extract var. rome beauty first dose (0.12 mg / kg), the second dose (0.24 mg / kg), and the third dose (0.49 mg / kg). By using one way ANOVA test obtained significant value of alpha 0.000 (P <0.000) that means there are significant differences in effectiveness between the groups. Based on pos hoc test showed  the significant differences between the positive control group to the treatment group apple peel extract var. rome beauty first dose, second dose  and third dose (P <0.05), meaning that apple peel extract var. rome beauty has effectiveness to SGPT levels. Thus the apple peel extract var.rome beauty has effectiveness to SGPT level of rat that induced by CCl4.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum

e-GIGI ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 238
Author(s):  
Stevia E. Nonutu ◽  
Damajanty H. C. Pangemanan ◽  
Christy N. Mintjelungan
Keyword(s):  
Side Effects ◽  
Treatment Options ◽  
Antibacterial Properties ◽  
Fusobacterium Nucleatum ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design

Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat

scholarly journals Dosis Efektif Enoxaparin dalam Mencegah Terjadinya Trombosis pada Anastomosis Arteri Femoralis Tikus

2017 ◽  
Vol 1 (2) ◽  
pp. 34
Author(s):  
I Made Suka Adnyana ◽  
Iswinarno Doso Saputro
Keyword(s):  
Free Flap ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Wistar Strain ◽  
Post Test

Tujuan: untuk mengetahui dosis efektif enoxaparin dalam mencegah terjadinya trombosis pada anastomosis mikrovaskular. Metode: penelitian ini bersifat eksperimental dengan rancangan the randomized post test only control group design. Terdapat 33 tikus jantan Rattus norvegicus Wistar strain yang dikelompokkan menjadi tiga perlakuan yaitu perlakuan A (enoxaparin 0,75 mg/kg), B (enoxaparin 1  mg/kg), dan C (kontrol). Tuck model anastomosis dilakukan pada arteri femoralis, kemudian luas trombus yang terjadi pada pembuluh darah dibandingkan dengan diameter lumen pembuluh darah diukur dengan graticule lens dan dinyatakan dalam persen. Hasil: trombus terbentuk pada semua subyek penelitian baik pada kelompok perlakuan maupun kontrol. Rerata persentase luas trombus pada kelompok enoxaparin 0,75 mg/kg adalah 24,3%, enoxaparin 1 mg/kg sebesar 19,8% dan kelompok NaCl 0,9% sebesar 79,4%. Terdapat perbedaan antara perlakuan pemberian enoxaparin dosis 0,75 mg/kg dan 1 mg/kg dengan kontrol, namun tidak ada perbedaan bermakna rerata persentase luas trombus diantara kelompok enoxaparin dosis 0,75 mg/kg dan dosis 1 mg/kg (p=0,624). Perlu dilakukan penelitian secara klinis guna melihat efektivitas enoxaparin dalam meningkatkan patensi anastomosis pada free flap maupun replantasi. Simpulan: pemberian enoxaparin dosis 0,75 mg/kg dan enoxaparin dosis 1 mg/kg secara subkutan efektif mengurangi persentase luas trombus pada anastomosis arteri femoralis tikus. Tidak terdapat perbedaan efektivitas yang bermakna dalam mengurangi persentase luas trombus pada anastomosis arteri femoralis tikus setelah diberikan dosis enoxaparin 0,75 mg/kg dan 1 mg/kg secara subkutan.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Effectiveness of Boiled Figs (Ficus carica L), Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia Calabura L.) on Decreasing SGOT and SGPT Serum in Male Wistar Strain Rats with Acute Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 744-755
Author(s):  
Grace Arni Yunita Hutajulu ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Catharanthus Roseus ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Wistar Strain ◽  
Muntingia Calabura

Acute hepatitis is inflammation of the liver. This disorder is usually caused by a virus or exposure to alcohol, drugs, toxic, or other pathogens. The purpose of this study was to determine the effectiveness of boiled figs leaves (Ficus carica L.), Rosy Periwinkle (Catharanthus roseus), and cherry (Muntingia Calabura L.) on decreasing serum levels of SGOT and SGPT. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 0.3 grams Figs (Ficus carica L.), 3.5 grams of rosy Periwinkle (Catharanthus roseus), and 3.5 grams of cherry (Muntingia Calabura L.) was given as much as 3.6 cc orally for 7 days to treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test was performed to compare SGOT and SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group, and negative control group (p <0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p <0.05). The conclusions of this study were mixed  boiled of Figs (Ficus carica L.), rosy Periwinkle (Catharanthus roseus), and and Cherry (Muntingia Calabura L.) had an effect in decreasing serum SGOT and SGPT in male rats with acute hepatitis model.

scholarly journals Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Sadakata Sinulingga ◽  
Subandrate Subandrate ◽  
Bebbi Arisya Kesumaputri ◽  
Galuh Anggraini
Keyword(s):  
Bleeding Time ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Piper Betle ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Group Design ◽  
Hemostatic Effect

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Mice’s tail was cut at diameter 3 mm and pretest bleeding time was counted. Mice’s tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

scholarly journals An IGF-1 Levels Decrease in SOPK-RI Model Mice After A Low-Carb Diet

2020 ◽  
Vol 9 (2) ◽  
pp. 325-331
Author(s):  
Ermawati Ermawati ◽  
Budi Santoso ◽  
Widjiati Widjiati ◽  
Hany Puspita Aryani
Keyword(s):  
Design Research ◽  
Polycystic Ovary ◽  
Negative Control Group ◽  
Positive Control ◽  
Vaginal Swab ◽  
Negative Control ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Control Group Design

The aim of this study is to analyze the IGF-1 levels decrease in mice modeled by Polycystic Ovary Syndrome Insulin Resistance post carbohydrate high in protein. True Experimental Design with a Posttest Control Group Design research using 36 tails, female Wistar Rattus Norvegicus Strains, 3 months, 100-200 grams and randomly divided into 3 groups, namely K-(n =12, negative control group), K+(n=12, positive control group) and KP(n=12, treatment group). SOPK-IR modeling is done by mice in the injection of the hormone testosterone provionate at a dose of 100 mg/kg BW for 28 days, on the last day after injection a vaginal swab is performed to identify the condition of diestrus. The measurement of IGF-1 levels used the Enzyme-Linked Immunosorbent Assay method. Data analysis techniques used ANOVA test and LSD post hoc test. The results obtained mean levels of IGF-1 at K- (234.126±32.568) ng/mL, K+ (169.662±30.150) ng/mL, KP (146.528±29.562) ng/mL (p=0.000)

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