scholarly journals PENGARUH VARIASI WAKTU PERENDAMAN SAMPEL DAN JUMLAH PELARUT HOMOGENASI TERHADAP PERSENTASE EKTRAK PROTEIN DAUN KELOR (Moringa oleifera)

2020 ◽  
Vol 7 (1) ◽  
pp. 51-56
Author(s):  
Meirita Sari ◽  
Methatias Ayu Moulina
Keyword(s):  
Immersion Time ◽  
Moringa Oleifera ◽  
Protein Extraction ◽  
Health Sector ◽  
Extraction Process ◽  
Food Plants ◽  
Leaf Protein ◽  
Protein Extract ◽  
Salting Out ◽  
Science And Education

This research is one form of utilization of food plants, namely Moringa (Moringa oleifera). M. oleifera can grow well in Bengkulu Province. The leaves of M. oleifera contain protein compounds that behave like lectins. This compound can be used as an antimicrobial material. The stages in this study include the extraction of M. oleifera leaf protein using salting in and salting out methods. This study aims to determine the effect of the immersion time and the amount of solvent in the homogenation stage extraction process on the percentage of protein extract. The results showed that the immersion time had a significant effect compared to the amount of solvent in the homogenation stage of Moringa oleifera leaf protein extraction. The longer the time needed, the greater the percentage of M. oleifera leaf protein extract. This research is useful for researchers, the public, the health sector, and the development of science and education.     Keywords: Moringa oleifera, Extraction, Protein

scholarly journals Optimization of the extraction and precipitation process of a leaf protein concentrate from Moringa oleifera Lam.

2022 ◽  
Vol 75 (1) ◽  
Author(s):  
Byron Patricio Pérez Simba ◽  
Javier Alberto Garrido Espinosa ◽  
Andrea Belén Endara Vargas ◽  
Andrea Carolina Landázuri Flores ◽  
Lucía de los Ángeles Ramírez Cárdenas
Keyword(s):  
Moringa Oleifera ◽  
Protein Extraction ◽  
Extraction Process ◽  
Raw Material ◽  
Leaf Protein ◽  
Precipitation Process ◽  
Randomized Design ◽  
Leaf Protein Concentrate ◽  
Influence Of Ph ◽  
Moringa Oleifera Lam

This study aimed to determine the best extraction and precipitation conditions of Moringa oleifera Lam. leaf protein. The influence of pH (10, 11, 12) and the concentration of NaCl (0, 0.25, 0.5) for the protein extraction process were studied through a Completely Randomized Design (CRD) with factorial arrange 32. The combination of pH 11 and 12 with 0 M NaCl had the best yield (P<0.05). The treatment of pH 11 without NaCl followed a precipitation stage for its purification, and the effect of different levels of pH (4, 4.5, 5) and temperature (40, 60, 80 °C) were evaluated using a CRD with factorial arrange 22 and 6 central points. The temperature did not affect the yield of the process in a significant way and the amount of precipitate was maximized at pH 4 and 4.5. From 100 g of the dry leaf, 7.26±0.19 g of protein was isolated with a recovery of 26.93±0.22 g 100 g-1 from the total protein. Due to their astringency and bitterness, consuming large amounts of Moringa oleifera Lam leaves is not a solution; therefore, obtaining a leaf proteinconcentrate could be useful for diverse applications in nutritional supplements, and as raw material for functional products development.

Coagulation of organic pollutants by Moringa oleifera protein molecules: In silico approach

2021 ◽  
Author(s):  
Victoria Teniola Adeleke ◽  
Adebayo A Adeniyi ◽  
David Lokhat
Keyword(s):  
Wastewater Treatment ◽  
Organic Pollutants ◽  
In Silico ◽  
Moringa Oleifera ◽  
Seed Protein ◽  
Clean Water ◽  
Protein Extract ◽  
Natural Coagulant ◽  
Global Challenge ◽  
Protein Molecules

Availability of clean water for various activities is a global challenge. Moringa oleifera (MO) seed protein extract has been identified as a natural coagulant for wastewater treatment. The mechanistic understanding...

scholarly journals A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Jianghao Du ◽  
Zhanyun Zhu ◽  
Junchang Yang ◽  
Jia Wang ◽  
Xiaotong Jiang
Keyword(s):  
Protein Structure ◽  
Comparative Study ◽  
Protein Extraction ◽  
Guanidine Hydrochloride ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Extraction Process ◽  
Ultraviolet Absorption Spectrum ◽  
Mural Paintings ◽  
The Impact

AbstractIn this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These agents were used to extract the residual proteins in the non-aged and thermal aged samples. The protein extraction efficiencies of different extracting agents were quantitatively determined by bicinchoninic acid (BCA) method, and then processed by multivariate analysis of variance (MANOVA). The impact of the extraction process on the protein structure was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet absorption spectrum (UV) and circular dichroism (CD). The results showed that, for both non-aged and aged samples, the extraction efficiency of 2 M guanidine hydrochloride (GuHCl) was significantly higher than the other five agents, with less damage to the protein structure during the extraction process.

scholarly journals Reverse Micelle Liquid-Liquid Extraction of a Pharmaceutical Product

2012 ◽  
Vol 545 ◽  
pp. 240-244 ◽  
Author(s):  
Siti Hamidah Mohd-Setapar ◽  
Siti Norazimah Mohamad-Aziz ◽  
N.H. Harun ◽  
S.H. Hussin
Keyword(s):  
Aqueous Solution ◽  
Aqueous Phase ◽  
Reverse Micelle ◽  
Surfactant Concentration ◽  
Protein Extraction ◽  
Biological Activities ◽  
Extraction Process ◽  
Pharmaceutical Product ◽  
Penicillin G ◽  
Reverse Micelle Extraction

Reverse micelle extraction has received considerable attention in recent years due to its ability to selectively solubilise solutes from an aqueous phase, and in the case of biomolecules to maintain their biological activities. The apparent success of research on protein extraction from the aqueous phase using reverse micelle provides motivation to study the solubilisation of antibiotic. The objective of this study is to investigate the extraction of antibiotic (penicillin G is chosen as model antibiotic) from aqueous solution (forward extraction) and from the reverse micelle to a new aqueous solution (backward extraction). Sodium di(2-ethylhexyl)sulfosuccinate (AOT) is chosen as the surfactant and isooctane as the organic solvent. The UV-Vis spectrophotometer is used to determine the mass of penicillin G in solution after the extraction process. The extraction is expected to be influenced by the initial penicillin G concentration, the salt type and concentration in the aqueous phase, pH, and surfactant concentration. It is expected that as penicillin is an interfacially active compound that will interacts with AOT surfactant, the interfacial association will be dependent on both pH and surfactant concentration.

scholarly journals Evaluation of effective protein extraction procedure to profile petiole of Moringa oleifera

2020 ◽  
Vol 7 (2) ◽  
pp. 214
Author(s):  
Zetty Amirah Zulkifli ◽  
Zaidah Rahmat
Keyword(s):  
Moringa Oleifera ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Extraction Procedure ◽  
Electrophoretic Pattern ◽  
Antioxidant Property ◽  
Extraction Methods ◽  
Protein Profiling ◽  
Sds Page

Moringa oleifera is widely known as multipurpose tree since all of its parts confer multiple functions. The leaf is highly favourable among consumers while the petiole is mostly wasted. There are numerous studies on the flavonoid and antioxidant property of the stem and twig. However, study on the petiole has never been done. There-upon, this study was conducted to develop protein profiling of the petiole. In this study, 6 different protein extraction methods were tested on the fresh petiole before its protein quantity and quality were checked via Bradford assay and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) respectively. The in-solution digestion was then done prior to LC-MS/MS analysis. The protein electrophoretic pattern from the SDS-PAGE proves that method 6 using Tris HCl buffer with incorporation of dithiothreitol (DTT) and phenylmethylsulfonyl fluoride (PMSF) confers the best quality of protein. It produced the highest number of visible individual bands compared to other methods. Meanwhile, 93 proteins were successfully identified via LCMS analysis where the protein, signal response and carbohydrate metabolism categories confer the highest percentage. High quality and content of the protein extracted from the petiole including the antioxidant, anticancer and antidiabetic protein identified suggested that consuming this part of the plant could enhance nutrients of human body.

scholarly journals Effect of extraction method on Curcuma xanthorrhiza oleoresin using solar dryer to concentration of curcuminoid, total phenol and antioxidant activty

2017 ◽  
Vol 14 (1) ◽  
pp. 1-9
Author(s):  
THERESIA AGNIEST PRICILLA VITANTI ◽  
KAWIJI KAWIJI ◽  
EDHI NURHARTADI
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Immersion Time ◽  
Size Variation ◽  
Extraction Process ◽  
Total Phenol ◽  
Raw Material ◽  
Powder Size ◽  
Solar Dryer ◽  
Curcuma Xanthorrhiza

Vitanti TAP, Kawiji, Edhi N. 2012. Effect of extraction method on Curcuma xanthorrhiza oleoresin using solar dryer to concentration of curcuminoid, total phenol, and antioxidant activity. Biofarmasi 14: 1-9. Curcuma (Curcuma xanthorrhiza Roxb.) is a type of drug plant that has high enough capacity of production in Indonesia. Generally, commerced in the form of fresh curcuma or processed product as simple as simplicia and curcuma powder. Processed products that could be developed is curcuma oleoresin. It is a mixture of essential oils and resins obtained from extraction process of curcuma powder using an organic solvent. Oleoresin has the same flavor and aroma to the extracted material. Due to these characteristics, it is used as a flavor and food coloring, other than as a raw material in pharmaceutical industry. In addition, it also contains active compounds which can support the utilization of drug and food industries. This study aims to determine whether the size of the powder, powdered curcuma immersion time, and interactions between them that can be influenced the content of curcuminoids, total phenol and antioxidant activity of curcuma oleoresin. Selection of solar dryers in the drying process is based on previously studied that compare the natural drying technique with a solar dryer, and the best results of those studies are shown in the solar dryer. This research using completely randomized design with two factors: the size variation of curcuma powder (60, 80 and 100 mesh) and immersion time variation (extraction) of curcuma powder (12, 24 and 36 hours). The results showed that the powder size of curcuma and immersion time has an effect on curcuminoid content, total phenol and antioxidant activity of curcuma oleoresin. However, there are no interaction between both factors. That is, the size and the immersion time of curcuma powder do not affect each other on the content of curcuminoid, total phenol and activity of antioxidant.

scholarly journals High throughput method development and optimised production of leaf protein concentrates with potential to support the agri-industry

2021 ◽  
Author(s):  
Ajay Iyer ◽  
Lisa Guerrier ◽  
Salomé Leveque ◽  
Charles S. Bestwick ◽  
Sylvia H. Duncan ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Extraction ◽  
Leaf Protein ◽  
Protein Recovery ◽  
Screening Methods ◽  
Extraction Technology ◽  
Protein Concentrates ◽  
Ethanol Precipitation

AbstractInvasive plants offer an interesting and unconventional source of protein and the considerable investment made towards their eradication can potentially be salvaged through their revalorisation. To identify viable sources, effective and high-throughput screening methods are required, as well as efficient procedures to isolate these components. Rigorous assessment of low-cost, high-throughput screening assays for total sugar, phenolics and protein was performed, and ninhydrin, Lever and Fast Blue assays were found to be most suitable owing to high reliability scores and false positive errors less than 1%. These assays were used to characterise invasive Scottish plants such as Gorse (Ulex europeans), Broom (Cystisus scoparius) and Fireweed (Chamaenerion angustifolium). Protein extraction (alkali-, heat- and enzyme assisted) were tested on these plants, and further purification (acid and ethanol precipitation, as well as ultrafiltration) procedures were tested on Gorse, based on protein recovery values. Cellulase treatment and ethanol precipitation gave the highest protein recovery (64.0 ± 0.5%) and purity (96.8 ± 0.1%) with Gorse. The amino acid profile of the purified protein revealed high levels of essential amino acids (34.8 ± 0.0%). Comparison of results with preceding literature revealed a strong association between amino acid profiles and overall protein recovery with the extraction method employed. The final purity of the protein concentrates was closely associated to the protein content of the initial plant mass. Leaf protein extraction technology can effectively raise crop harvest indices, revalorise underutilised plants and waste streams.

Characterisation of black soldier fly larva protein before and after conjugation by the Maillard reaction

2021 ◽  
pp. 1-16
Author(s):  
B. Zozo ◽  
M.M. Wicht ◽  
V.V. Mshayisa ◽  
J. van Wyk
Keyword(s):  
Maillard Reaction ◽  
Protein Extraction ◽  
Structural Difference ◽  
Principal Component ◽  
Protein Molecule ◽  
Nutritional Composition ◽  
Gravimetric Analysis ◽  
Protein Extract ◽  
Scanning Calorimetry ◽  
Black Soldier Fly

Black soldier fly has been proposed as an alternative protein source sustainable for both food and feed due to its nutritional composition. The functional properties of this protein can be improved by inducing the Maillard reaction (MR) in protein-sugar mixtures. This study focused on the conjugation and characterisation of black soldier fly larvae (BSFL) proteins and conjugates. The defatted BSFL larvae were subjected to protein extraction at an alkaline pH. The protein extract was then conjugated with glucose. The BSFL protein and glucose were mixed at (2:1 w/w, pH 9), incubated at 50, 70, and 90 °C for 30, 60, 90 and 120 min. The products obtained were then characterised and compared. The changes were confirmed by universal attenuated total reflectance Fourier-transform infrared spectroscopy (UATR-FTIR), scanning electron microscopy, thermal gravimetric analysis and differential scanning calorimetry. UATR-FTIR combined with principal component analysis monitored the protein-sugar conjugates, to show the structural difference among heated proteins and conjugates. The heating treatments resulted in the unfolding and reduction of the protein molecule aggregation. The protein extract from the larvae was rich in protein content (67±0.78%) and displayed good essential amino acids (EAA) in sufficient quantities to meet the dietary requirements for humans. The EAA quantities of the conjugates decreased due to the MR treatment. The conjugates showed a significant decrease in the lysine content as a function of reaction temperature and time at 90 °C and 120 min, respectively. FTIR indicated that the amide I and II bands of the protein were altered by the MR. The increased Tmax (the temperature at which decomposition is completed) demonstrated that the conjugation of the protein with glucose improved the thermal stability, remarkably. These results suggested that MR with glucose can be a promising way to improve the thermal properties of BSFL protein.

scholarly journals A Novel Green Extraction Technique for Extracting Flavonoids from Folium nelumbinis by Changing Osmosis Pressure

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4192
Author(s):  
Hai-Yan Fang ◽  
Ying-Qin Wei ◽  
Meng-Li Zhang ◽  
Wei Liu
Keyword(s):  
Extraction Efficiency ◽  
Chemical Potential ◽  
Volume Fraction ◽  
Antioxidant Activities ◽  
Morphological Changes ◽  
Extraction Process ◽  
Extraction Technique ◽  
Salting Out ◽  
Digital Microscope ◽  
Main Factor

A new green and sustainable extraction technique, namely osmosis extraction (OE), was developed for efficient extracting flavonoids from Folium nelumbinis by changing the osmotic pressure. The antioxidant activities of the extracted flavonoids were also evaluated. Ethanol and ammonium sulfate were selected for the OE system because they are environmentally friendly. The maximum flavonoids concentration in the top phase was obtained with an ethanol volume fraction of 42.0% and the salt mass of 1.9 g. The kinetic behavior of the extraction process showed that OE had higher efficiencies especially coupled with ultrasonication due to the accompanying and serious morphological changes of Folium nelumbinis cells observed by digital microscope and nano-computed tomography (nano-CT). Results of morphological and anatomical features showed that the higher intracellular chemical potential made the cell expand and even led to bursting. The results also showed that the extraction efficiency of flavonoids with high antioxidant activities was higher than that of the traditional method. The interface effect enhanced the extraction during the salting-out extraction and osmosis was the main factor that improved the extraction efficiency.

Sign in / Sign up

Export Citation Format

Share Document