AVALIAÇÃO DA ATIVIDADE ANTIMICROBIANA DE SURFACTANTE VERDE FRENTE AO CONSÓRCIO DE BACTÉRIAS PSEUDOMONAS AERUGINOSA E BACILLUS CEREUS

2021 ◽  
pp. 49-56
Author(s):  
Maria Eduarda Pereira da Silva ◽  
Anderson Oliveira de Medeiros ◽  
Maria da Glória Conceição Silva ◽  
Leonie Asfora Sarubbo
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus

PENGUJIAN KONSORSIUM BAKTERI ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT BERCAK DAUN CERCOSPORA DAN VIRUS KUNING PADA TANAMAN CABAI MERAH BESAR (Capsicum annum L.) DI KECAMATAN DAMPIT KABUPATEN MALANG

2021 ◽  
Vol 9 (3) ◽  
pp. 107-114
Author(s):  
Diyana Lestari ◽  
Luqman Qurata Aini
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Duncan Multiple Range Test ◽  
Capsicum Annum ◽  
Range Test

Penyakit virus kuning dan bercak daun Cercospora merupakan dua penyakit penting yang menjadi kendala dalam budidaya cabai merah besar. Penelitian ini bertujuan untuk menguji konsorsium bakteri antagonis Pseudomonas aeruginosa dan Bacillus cereus dalam mengendalikan penyakit virus kuning dan bercak daun Cercospora pada cabai merah besar serta pengaruhnya terhadap pertumbuhan tanaman cabai merah besar sendiri. Penelitian dilaksanakan pada bulan Oktober 2020 - Desember 2020 di lahan tumpangsari bawang merah dan cabai, Desa Ubalan, Kecamatan Dampit, Kabupaten Malang. Persiapan penelitian meliputi survei lokasi, persiapan alat dan bahan, pengacakan perlakuan, dan penentuan tanaman sampel. Pelaksanaan penelitian meliputi pengaplikasian larutan isolat bakteri antagonis di lapang, pengamatan mingguan, dan pengolahan data. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) dengan 9 perlakuan dan 3 ulangan. Data dianalisis menggunakan sidik ragam ANOVA dan uji lanjut DMRT (Duncan Multiple Range Test) pada taraf 5% menggunakan software DSAASTAT. Konsorsium P7 (Pseudomonas aeruginosa + Bacillus cereus 13) lebih efektif dalam menekan penyakit virus kuning, sedangkan perlakuan konsorsium P8 (Bacillus cereus 12 + Bacillus cereus 13) lebih efektif dalam menekan penyakit bercak daun Cercospora. Perlakuan konsorsium tidak memberikan pengaruh terhadap tinggi dan jumlah daun. Namun, konsorsium P7 mampu meningkatkan bobot tanaman cabai meskipun secara statistik tidak berbeda dengan perlakuan lainnya.

scholarly journals Uji Aktivitas Antibakteri dari Ekstrak dan Fraksi Daging Buah Mangga Kasturi (Mangifera Casturi) terhadap Bakteri Pseudomonas Aeruginosa dan Bacillus Cereus

2021 ◽  
Vol 49 (2) ◽  
pp. 113-122
Author(s):  
Meliana Meliana ◽  
Sogandi Sogandi ◽  
Ekajayanti Kining
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Mangifera Indica ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry

Kasturi (Mangifera casturi) is a typical plant of South Kalimantan which has only been used for consumption because of its distinctive taste and aroma. Mangifera indica, which is in the same genus as Mangifera casturi, shows antioxidant, antibacterial, antifungal, and antidiarrheal activity, so it is suspected that this plant has the same activity. Crude methanol extract from the meat of Casturi mango was fractionated using n-hexane, ethyl acetate, and methanol-water. Testing for antibacterial activity and minimum inhibitory concentration (MIC) were carried out using the disk diffusion method (Kirby-Bauer). The positive control used was Chloramphenicol because it has an antibacterial range that is rather broad and the negative control used was 10% DMSO. The fraction that had the greatest antibacterial activity was the ethyl acetate fraction; with the formation of a clear zone of 7.98mm against Pseudomonas aeruginosa and 7.52mm against Bacillus cereus, with a minimum inhibitory concentration of 2mg/mL. The next test was a cell leakage analysis which revealed an increase in absorbance at MIC 1 and MIC 2. The Gas Chromatography – Mass Spectrometry (GC-MS) results from the ethyl acetate fraction revealed the presence of phenolic, flavonoid, and glycoide content, with the highest compound concentration of 5-Hydroxymethylfurfural. Keywords: Antibacterial, Extract, Ethyl acetate, fraction, Mangifera casturi Abstrak Kasturi (Mangifera casturi) merupakan tumbuhan khas Kalimantan Selatan yang selama ini hanya dimanfaatkan untuk dikonsumsi karena rasa dan aromanya yang khas. Mangifera indica yang masih satu genus dengan Mangifera casturi menunjukkan aktivitas antioksidan, antibakteri, antijamur, dan antidiare sehingga diduga tanaman ini memiliki aktivitas yang sama. Ekstrak kasar metanol daging buah mangga kasturi difraksinasi dengan menggunakan n-heksan, etil asetat, dan metanol-air. Pengujian aktivitas antibakteri dan kadar hambat minimum dilakukan dengan menggunakan metode difusi cakram (Kirby- Bauer). Kontrol positif yang digunakan adalah Chloramfenikol karena memiliki spektrum antibakteri yang cukup luas dan kontrol negatif yang digunakan adalah DMSO 10%. Fraksi yang memiliki aktivitas antibakteri terbesar pada penelitian ini adalah fraksi etil asetat dengan terbentuknya zona bening sebesar 7,98mm terhadap Pseudomonas aeruginosa dan 7,52mm terhadap Bacillus cereus dengan kadar hambat minimum 2mg/mL. Uji selanjutnya adalah uji kebocoran sel yang menyatakan adanya peningkatan absorbansi pada KHM 1 dan KHM 2. Hasil pemeriksaan Gas Chromatography – Mass Spectrometry (GCMS) dari fraksi etil asetat menyatakan adanya kandungan fenolik, flavonoid, dan glikosida dengan senyawa tertinggi yaitu 5-Hydroxymethylfurfural. Kata kunci: Antibakteri, Ekstrak, Etil asetat, Fraksi, Mangifera castur

scholarly journals Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

2021 ◽  
Author(s):  
Davood Gheidari ◽  
Morteza Mehrdad ◽  
Saloomeh Maleki ◽  
Samanesadat Hosseini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Limited Area ◽  
X Ray Diffraction ◽  
X Ray ◽  
Inhibition Concentration ◽  
Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

scholarly journals POTENSI KULTUR CAMPURAN BAKTERI ENDOFIT SEBAGAI PEMACU PERTUMBUHAN BIBIT TANAMAN KARET

2014 ◽  
Vol 32 (2) ◽  
pp. 129 ◽  
Author(s):  
Umi Hidayati ◽  
Iswandi Anas Chaniago ◽  
Abdul Munif ◽  
Siswanto Siswanto ◽  
Dwi Andreas Santosa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Scanning Electron

Bakteri endofit adalah bakteri yang hidup dalam jaringan tanaman, dapat diisolasi melalui sterilisasi permukaan jaringan tanaman. Isolasi bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan sangat penting dilakukan. Pembuatan kultur campuran dari bakteri endofit diharapkan meningkatkan potensi dalam memacu pertumbuhan yang dapat meningkatkan kualitas bibit batang bawah tanaman karet. Penelitian ini dilakukan dengan tujuan mendapatkan kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Lima bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan yaitu Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, Brachybacterium paraconglomeratum LPD74, bacterium (bakteri tidak dikenal) LPD76, dan Providencia vermicola KPA38, diuji kompatibilitas untuk mendapatkan kultur campuran yang dapat meningkatkan pertumbuhan bibit batang bawah PB 260. Semua bakteri endofit terpilih kompatibel satu dengan yang lain. Aplikasi kultur campuran untuk meningkatkan pertumbuhan bibit batang bawah PB 260 memberikan hasil 2 kultur campuran terbaik. Kultur campuran 1 terdiri 2 spesies bakteri yaitu Brachybacterium paraconglomeratum LPD74 dan Providencia vermicola KPA38.  Kultur campuran 2 terdiri 3 spesies bakteri yaitu  Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, dan Brachybacterium paraconglomeratum LPD74. Bakteri endofit mampu masuk ke planlet bibit karet microcutting yang dibuktikan dengan Scanning Electron Microscopy. Diterima : 19 Mei 2014; Direvisi : 30 Mei 2014; Disetujui : 21 Juni 2014  How to Cite : Hidayati, U., Chaniago, I. A., Munif, A., Siswanto., & Santosa, D. A. (2014). Potensi kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Jurnal Penelitian Karet, 32(2), 129-138. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/159

scholarly journals Comparative Evaluation of Antimicrobial Activity and Composition of Rose Oils from Various Geographic Origins, in Particular Bulgarian Rose Oil

2008 ◽  
Vol 3 (7) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Velizar Gochev ◽  
Katrin Wlcek ◽  
Gerhard Buchbauer ◽  
Albena Stoyanova ◽  
Anna Dobreva ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Essential Oils ◽  
Bacillus Cereus ◽  
Phenolic Compound ◽  
Comparative Evaluation ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Geographic Origins ◽  
Rose Oil

In the present study we evaluated the composition and antimicrobial activity of various rose oils from Bulgaria, Turkey, Morocco, Iran and China against three Gram-positive, three Gram-negative bacteria and two yeasts. The composition of the studied essential oils was determined by GC and GC/MS. Citronellol was the major compound of all the oil samples: 31.7% (Chinese oil); 32.6% (Iranian oil); 33.6% (Moroccan oil); 34.9% (Bulgarian oil) and 38,7 % (Turkish oil). For the Bulgarian oil we could determine the highest activity against all microorganisms. Bacillus cereus ATCC 11778 was the most sensitive strain to Bulgarian rose oil (MCC 128 μg/mL) and Pseudomonas aeruginosa ATCC 9627 and P. fluorescens were more resistible strains (MCC 4096 μg/mL). Antimicrobial activity of rose oils is mainly due to the action of oxygenated acyclic monoterpenes citronellol, geraniol, nerol and linalool, their acetate derivatives and the phenolic compound eugenol.

scholarly journals Imipenem as substrate and inhibitor of β-lactamases

1988 ◽  
Vol 253 (2) ◽  
pp. 323-328 ◽  
Author(s):  
J Monks ◽  
S G Waley
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Reversible Reaction ◽  
Clinical Use ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Class A ◽  
Class C ◽  
Kinetics Of ◽  
Carbapenem Antibiotic

The interaction between imipenem, a carbapenem antibiotic, and two representative beta-lactamases has been studied. The first enzyme was beta-lactamase I, a class-A beta-lactamase from Bacillus cereus; imipenem behaved as a slow substrate (kcat. 6.7 min-1, Km 0.4 mM at 30 degrees C and at pH 7) that reacted by a branched pathway. There was transient formation of an altered species formed in a reversible reaction; this species was probably an acyl-enzyme in a slightly altered, but considerably more labile, conformation. The kinetics of the reaction were investigated by measuring both the concentration of the substrate and the activity of the enzyme, which fell and then rose again more slowly. The second enzyme was the chromosomal class-C beta-lactamase from Pseudomonas aeruginosa; imipenem was a substrate with a low kcat. (0.8 min-1) and a low Km (0.7 microM). Possible implications for the clinical use of imipenem are considered.

scholarly journals Antimicrobial Property of Extracts of Indian Lichen against Human Pathogenic Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Priya Srivastava ◽  
D. K. Upreti ◽  
T. N. Dhole ◽  
Apurva K. Srivastava ◽  
Meghanand T. Nayak
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Streptococcus Faecalis ◽  
Human Pathogenic Bacteria ◽  
Usnea Ghattensis

Context.Usnea ghattensisG. Awasthi (Usneaceae) endemic fruticose lichen found growing luxuriantly in Northern Western Ghats of India, it also contains Usnic acid as a major chemical and tested against some human pathogenic bacteria.Objective. To explore antimicrobial properties ofUsnea ghattensisagainst some human pathogenic bacteria.Materials and Methods. The lichen was extracted in acetone, methanol, and ethanol.In vitroantimicrobial activity was tested initially byKirby-Bauertechnique of disc diffusion method and was confirmed by minimum inhibitory concentration using Broth microdilution method according to the NCCLS guidelines.Results. Ethanol extract was most effective againstBacillus cereusandPseudomonas aeruginosawith a zone of inhibition 29.8 ± 0.6 mm and 12.3 ± 0.5 mm diameters at a concentration of 0.2 mg/mL. Acetone and methanol extract demonstrated almost similar activity againstStaphylococcus aureusand the zone of inhibition was 24.6 ± 0.5 and 24.7 ± 0.4 mm. Only methanol extract was showing activity againstStreptococcus faecaliswith a 13.5 ± 0.8 mm zone. MIC value noted againstStaphylococcus aureusandStreptococcus faecaliswas 6.25 μg/mL and 25 μg/mL, whereas againstBacillus cereusandPseudomonas aeruginosa, MIC calculated was 3.125 μg/mL and 200 μg/mL, respectively.Conclusion. The present study demonstrates the relatively higher activity of this lichen against not only gram (+) but significantly also against gram (−) bacteria. This indicates that this lichen might be a rich source of effective antimicrobial agents.

scholarly journals Avaliação de Procedimentos Operacionais Padronizados Implementados na Produção e Manipulação de Fórmulas Infantis em Hospital Federal

2021 ◽  
Vol 25 (2) ◽  
pp. 128-135
Author(s):  
Mariana Fernandes Garcia da Silva ◽  
Maria das Graças Gomes de Medeiros ◽  
Robson Maia Franco ◽  
Maria Carmela Kasnowski Holanda Duarte ◽  
Sergio Girão Barroso
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Yersinia Enterocolitica ◽  
Infant Formulas ◽  
Salmonella Spp ◽  
Total Coliforms ◽  
Mesophilic Bacteria ◽  
Enterococcus Spp ◽  
Standard Operating

A contaminação de fórmulas infantis pode ocorrer quando os manipuladores cometem falhas operacionais ou falhas na adequação do ambiente de preparo. O objetivo da pesquisa foi avaliar a eficácia dos procedimentos operacionais padronizados executados na área de produção de fórmulas lácteas de um Hospital. Realizou-se uma pesquisa aplicada, descritiva e explicativa com avaliação do fluxo operacional e das condições físico funcionais, análise do comportamento dos manipuladores e pesquisas microbiológicas de Coliformes totais, Escherichia coli, Bactérias aeróbias mesófilas, Bacillus cereus, Yersinia enterocolitica, Listeria monicytogenes, Clostridium perfrigens, Clostridium Sulfito Redutor, Staphylococcus coagulase positiva, Salmonella spp., Enterococcous spp, Enterobacter sakazakii, Pseudomonas aeruginosa e fungos em superfícies de fórmulas infantis e água. Os procedimentos operacionais padronizados são executados em todas as etapas do processo, entretanto, foram observadas falhas humanas. Na avaliação físico-uncional, 92% estavam em conformidade; na pesquisa microbiológica de superfícies, 12% de resultados deram presenças para Enterococcus spp., Coliformes totais, Escherichia coli e Bactérias heterotróficas aeróbias mesófilas. Nas fórmulas infantis, 8,33% dos resultados mostraram presença para Coliformes totais, Bactérias heterotróficas aeróbias mesófilas e Fungos. Os procedimentos implantados asseguram a qualidade sanitária das fórmulas, mas se verificou a necessidade de treinamento dos manipuladores. Palavras-chave: Doenças Transmitidas por Alimentos. Fórmulas Infantis. Segurança Alimentar e Nutricional. Risco Sanitário. Abstract Infant formulas contamination may occur when handlers commit operational failures or failures in the environment adequacy. The objective of this research was evaluate the effectiveness of standard operating procedures performed in the production area of milk formulations of a Hospital. An applied, descriptive and explanatory research was conducted with evaluation of operational flow and physical functional conditions, analysis of the manipulators’s behavior and microbiological investigations of MPN of total Coliforms, Escherichia col and Enterococcus spp., Count of Bacillus cereus/g, Clostridium sulfite reducer, molds and yeasts, Staphylococcus coagulase positive, aerobic mesophilic bacteria and Cronobacter sakazakii, research of Yersinia enterocolitica, Listeria monocytogenes, Salmonella spp and Pseudomonas aeruginosa on surfaces, infant formulas and water. Standard operating procedures were performed at all the process stages, however, human failures have been observed. In the physical functional evaluation, 92% was in compliance; on the surface microbiological analysis, 12% of the results present Enterococcus spp., Total Coliforms, Escherichia coli and aerobic mesophilic bacteria. In infant formulas, 8.33% results showed presence for Total Coliforms, aerobic mesophilic bacteria and Fungi. The implanted procedures assured the formulas sanitary quality, but the necessity of the manipulators’s training was verified. Keywords: Foodborne Diseases. Infant Formulas. Food and Nutrition Security. Health Risk.

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