scholarly journals Analytical evaluation of breath acetone tubes

2020 ◽  
Vol 61 (2) ◽  
pp. 125-131
Author(s):  
Anastasia M. Ivanova ◽  
◽  
Elena O. Kolomina ◽  
Keyword(s):  
Glass Tube ◽  
Diagnostic Tools ◽  
Exhaled Breath ◽  
Acetone Concentration ◽  
Non Invasive ◽  
Sensitivity Assessment ◽  
Breath Analyzer ◽  
Metabolic Conditions ◽  
Breath Acetone ◽  
Color Gradients

Breath analysis provides opportunities for further development of precise and quick non-invasive diagnostic tools. The important example is the monitoring of metabolic flexibility through the acetone levels in an exhale. Metabolic changes may cause such pathological conditions as metabolic syndrome, type 2 diabetes mellitus and obesity. It is proven that breath acetone levels can indicate the states of ketosis or ketoacidosis. The development of sensitive, selective and easy-to-use tests for breath acetone is a step to personalized diagnostics, preliminary diagnosis and therapeutic control The aim of the research was to evaluate analytical characteristics of acetone breath tubes intended for non-invasive monitoring of metabolic state. The test is designed as an easy-to-blow glass tube, comprising a chemical reagent highly sensitive to acetone. The reagent changes its color from yellow to magenta depending on acetone concentrations. Sensitivity assessment was performed by lab simulation of an exhaled breath with various acetone concentrations. The acetone levels corresponded to a range associated with various metabolic conditions and were controlled by titrimetric method and a portable breath analyzer. Additionally, specificity to a target gas in the presence of water and ethanol vapors was assessed. The results showed a correlation between the acetone concentration and the color gradients of the acetone sensitive reagent. The tubes show no reactions towards water and ethanol vapors.

scholarly journals Vacuum Ultraviolet Absorption Spectroscopy Analysis of Breath Acetone Using a Hollow Optical Fiber Gas Cell

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 478
Author(s):  
Yudai Kudo ◽  
Saiko Kino ◽  
Yuji Matsuura
Keyword(s):  
Body Fat ◽  
Vacuum Ultraviolet ◽  
Exhaled Breath ◽  
Hollow Core ◽  
Gas Cell ◽  
Human Breath ◽  
Healthy Human ◽  
Acetone Concentration ◽  
Core Fiber ◽  
Breath Acetone

Human breath is a biomarker of body fat metabolism and can be used to diagnose various diseases, such as diabetes. As such, in this paper, a vacuum ultraviolet (VUV) spectroscopy system is proposed to measure the acetone in exhaled human breath. A strong absorption acetone peak at 195 nm is detected using a simple system consisting of a deuterium lamp source, a hollow-core fiber gas cell, and a fiber-coupled compact spectrometer corresponding to the VUV region. The hollow-core fiber functions both as a long-path and an extremely small-volume gas cell; it enables us to sensitively measure the trace components of exhaled breath. For breath analysis, we apply multiple regression analysis using the absorption spectra of oxygen, water, and acetone standard gas as explanatory variables to quantitate the concentration of acetone in breath. Based on human breath, we apply the standard addition method to obtain the measurement accuracy. The results suggest that the standard deviation is 0.074 ppm for healthy human breath with an acetone concentration of around 0.8 ppm and a precision of 0.026 ppm. We also monitor body fat burn based on breath acetone and confirm that breath acetone increases after exercise because it is a volatile byproduct of lipolysis.

scholarly journals Breath Analysis Using eNose and Ion Mobility Technology to Diagnose Inflammatory Bowel Disease—A Pilot Study

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 55 ◽  
Author(s):  
Tiele ◽  
Wicaksono ◽  
Kansara ◽  
Arasaradnam ◽  
Covington
Keyword(s):  
Inflammatory Bowel Disease ◽  
Pilot Study ◽  
Ion Mobility ◽  
Bowel Disease ◽  
Breath Analysis ◽  
Diagnostic Tools ◽  
Exhaled Breath ◽  
Faecal Calprotectin ◽  
Non Invasive ◽  
Inflammatory Bowel

Early diagnosis of inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), remains a clinical challenge with current tests being invasive and costly. The analysis of volatile organic compounds (VOCs) in exhaled breath and biomarkers in stool (faecal calprotectin (FCP)) show increasing potential as non-invasive diagnostic tools. The aim of this pilot study is to evaluate the efficacy of breath analysis and determine if FCP can be used as an additional non-invasive parameter to supplement breath results, for the diagnosis of IBD. Thirty-nine subjects were recruited (14 CD, 16 UC, 9 controls). Breath samples were analysed using an in-house built electronic nose (Wolf eNose) and commercial gas chromatograph–ion mobility spectrometer (G.A.S. BreathSpec GC-IMS). Both technologies could consistently separate IBD and controls [AUC ± 95%, sensitivity, specificity], eNose: [0.81, 0.67, 0.89]; GC-IMS: [0.93, 0.87, 0.89]. Furthermore, we could separate CD from UC, eNose: [0.88, 0.71, 0.88]; GC-IMS: [0.71, 0.86, 0.62]. Including FCP did not improve distinction between CD vs UC; eNose: [0.74, 1.00, 0.56], but rather, improved separation of CD vs controls and UC vs controls; eNose: [0.77, 0.55, 1.00] and [0.72, 0.89, 0.67] without FCP, [0.81, 0.73, 0.78] and [0.90, 1.00, 0.78] with FCP, respectively. These results confirm the utility of breath analysis to distinguish between IBD-related diagnostic groups. FCP does not add significant diagnostic value to breath analysis within this study.

scholarly journals The Use of Breath Analysis for Diagnosing COVID-19: Opportunities, Challenges, and Considerations for Future Pandemic Responses

2021 ◽  
pp. 1-16
Author(s):  
Mustafa Abumeeiz ◽  
Lauren Elliott ◽  
Phillip Olla
Keyword(s):  
Public Health ◽  
Artificial Intelligence ◽  
Large Scale ◽  
Breath Analysis ◽  
Diagnostic Tools ◽  
Exhaled Breath ◽  
Analysis Techniques ◽  
Non Invasive ◽  
Volatile Organic ◽  
Broad Scale

Abstract Due to the COVID-19 pandemic, there is currently a need for accurate, rapid, and easy-to-administer diagnostic tools to help communities manage local outbreaks and assess the spread of disease. The use of Artificial Intelligence within the domain of breath analysis techniques has shown to have potential in diagnosing a variety of diseases such as cancer and lung disease by analyzing volatile organic compounds (VOCs) in exhaled breath. This combined with their rapid, easy-to-use, and non-invasive nature makes them a good candidate for use in diagnosing COVID-19 in large scale public health operations. However, there remains issues with their implementation when it comes to the infrastructure currently available to support their use on a broad scale. This includes issues of standardization, and whether or not a characteristic VOC pattern can be identified for COVID-19. Despite these difficulties, breathalysers offer potential to assist in pandemic responses and their use should be investigated.

scholarly journals Breath acetone concentration: too heterogeneous to constitute a diagnosis or prognosis biomarker in heart failure? A systematic review and meta-analysis

2021 ◽  
Author(s):  
Fares Gouzi ◽  
Diba Ayache ◽  
Christophe Hedon ◽  
Nicolas Molinari ◽  
Aurore Vicet
Keyword(s):  
Heart Failure ◽  
Systematic Review ◽  
New York ◽  
Meta Analysis ◽  
Heart Association ◽  
Good Prognosis ◽  
Exhaled Breath ◽  
Acetone Concentration ◽  
Diagnosis And Prognosis ◽  
Breath Acetone

Abstract Introduction: Exhaled breath acetone (ExA) has been investigated as a biomarker for heart failure (HF). Yet, barriers to its use in the clinical field have not been identified. The aim of this systematic review and meta-analysis was to assess the ExA heterogeneity and factors of variability in healthy controls (HC), to identify its relations with HF diagnosis and prognostic factors and to assess its diagnosis and prognosis accuracy in HF patients. Methods: A systematic search was conducted in PUBMED and Web of Science database. All studies with HC and HF patients with a measured ExA were included and studies providing ExA’s diagnosis and prognosis accuracy were identified. Results: Out of 971 identified studies, 18 studies involving 833 HC and 1009 HF patients were included in the meta-analysis. In HC, ExA showed an important heterogeneity (I²=99%). Variability factors were fasting state, sampling type and analytical method. The mean ExA was 1.89 times higher in HF patients vs. HC (782 [531-1032] vs. 413 [347-478] ppbv; p<0.001). One study showed excellent diagnosis accuracy, and one showed a good prognosis value. ExA correlated with New York Heart Association (NYHA) dyspnea (p<0.001) and plasma brain natriuretic peptide (p<0.001). Studies showed a poor definition and reporting of included subjects. Discussion: Despite the between-study heterogeneity in HC, the evidence of an excellent diagnosis and prognosis value of ExA in HF from single studies can be extended to clinical populations worldwide. Factors of variability (ExA procedure and breath sampling) could further improve the diagnosis and prognosis values of this biomarker in HF patients.

Acetone Bio-Sniffer (Gas-Phase Biosensor) for Monitoring of Human Volatile Using Enzymatic Reaction of Secondary Alcohol Dehydrogenase

2021 ◽  
Vol 6 (1) ◽  
pp. 45
Author(s):  
Takahiro Arakawa ◽  
Ming Ye ◽  
Kenta Iitani ◽  
Koji Toma ◽  
Kohji Mitsubayashi
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gas Phase ◽  
Secondary Alcohol ◽  
Enzymatic Reaction ◽  
Flow Cell ◽  
Exhaled Breath ◽  
Acetone Vapor ◽  
Acetone Concentration ◽  
Secondary Alcohol Dehydrogenase ◽  
Breath Acetone

We developed a highly sensitive acetone bio-sniffer (gas-phase biosensor) based on an enzyme reductive reaction to monitor breath acetone concentration. The acetone bio-sniffer device was constructed by attaching a flow-cell with nicotinamide adenine dinucleotide (NADH)-dependent secondary alcohol dehydrogenase (S-ADH) immobilized membrane onto a fiber-optic NADH measurement system. This system utilizes an ultraviolet light emitting diode as an excitation light source. Acetone vapor was measured as the fluorescence of NADH consumption by the enzymatic reaction of S-ADH. A phosphate buffer that contained oxidized NADH was circulated into the flow-cell to rinse the products and the excessive substrates from the optode; thus, the bio-sniffer enables the real-time monitoring of acetone vapor concentration. A photomultiplier tube detects the change in the fluorescence emitted from NADH. The relationship between the fluorescence intensity and acetone concentration was identified to be from 20 ppb to 5300 ppb. This encompasses the range of concentration of acetone vapor found in the breath of healthy people and of those suffering from disorders of carbohydrate metabolism. Then, the acetone bio-sniffer was used to monitor the exhaled breath acetone concentration change before and after a meal. When the sensing region was exposed to exhaled breath, the fluorescence intensity decreased and reached saturation immediately. Then, it returned to the initial state upon cessation of the exhaled breath flow. We anticipate its future use as a non-invasive analytical tool for the assessment of lipid metabolism in exercise, fasting and diabetes mellitus.

Breath acetone analyzer: diagnostic tool to monitor dietary fat loss

1993 ◽  
Vol 39 (1) ◽  
pp. 87-92 ◽  
Author(s):  
S K Kundu ◽  
J A Bruzek ◽  
R Nair ◽  
A M Judilla
Keyword(s):  
Weight Loss ◽  
Dietary Fat ◽  
Diabetes Management ◽  
Exhaled Breath ◽  
Blue Color ◽  
Gas Analyzer ◽  
Acetone Concentration ◽  
Fat Loss ◽  
Weight Loss Programs ◽  
Breath Acetone

Abstract Acetone, a metabolite of fat catabolism, is produced in excessive amounts in subjects on restricted-calorie weight-loss programs. Breath acetone measurements are useful as a motivational tool during dieting and for monitoring the effectiveness of weight-loss programs. We have developed a simple, easy-to-read method that quantifies the amount of acetone in a defined volume of exhaled breath after trapping the sample in a gas-analyzer column. The concentration of acetone, as measured by the length of a blue color zone in the analyzer column, correlates with results obtained by gas chromatography. Using the breath acetone analyzer to quantify breath acetone concentrations of dieting subjects, we established a correlation between breath acetone concentration and rate of fat loss (slope 52.2 nmol/L per gram per day, intercept 15.3 nmol/L, n = 78, r = 0.81). We also discussed the possibility of using breath acetone in diabetes management.

scholarly journals Advanced Diagnostic Technology of Volatile Organic Compounds Real Time analysis Analysis From Exhaled Breath of Gastric Cancer Patients Using Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry

2021 ◽  
Vol 11 ◽  
Author(s):  
Yoon Ju Jung ◽  
Ho Seok Seo ◽  
Ji Hyun Kim ◽  
Kyo Young Song ◽  
Cho Hyun Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gastric Cancer ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Home Healthcare ◽  
Screening Tools ◽  
Diagnostic Tools ◽  
Exhaled Breath ◽  
Non Invasive ◽  
Volatile Organic

BackgroundScreening endoscopy is considered to be the most accurate tool for early detection of gastric cancer, but it is both invasive and costly. It is therefore essential to develop cost-effective and non-invasive diagnostic tools for gastric cancer. The aim of this study is to investigate the presence of certain volatile organic compounds (VOCs) associated with gastric cancer and to survey the usefulness of VOCs as screening tools of gastric cancer.MethodsThe present study was conducted prospectively to identify the relationship between gastric cancer and specific VOCs quantified by mass spectrometry. Exhaled breath samples from a total of 43 participants were analysed. This study was approved by the Institutional Review Board of the College of Medicine, Catholic University of Korea (KC16TISI0598), and registered to clinical research information service (KCT0004356).ResultsNine VOCs differed significantly between the control and cancer patient groups. When participants were divided into control, early gastric cancer (EGC), and advanced gastric cancer (AGC) groups, seven VOCs remained significantly different. Of these, four (propanal, aceticamide, isoprene and 1,3 propanediol) showed gradual increases as cancer advanced, from normal control to EGC to AGC. In receiver operating characteristic curves for these four VOCs, the area under the curve for gastric cancer prediction was highest (0.842) when more than two VOCs were present.ConclusionsThe present study offers potential directions for non-invasive gastric cancer screening, and may inspire advanced diagnostic technologies in the era of smart home healthcare. However, despite the high accuracy, cancer-specific VOCs from several studies on different populations, and analytic methods show inconsistency, it is necessary to establish standards for each analytical method, and to validate on each population.

scholarly journals Exhaled Breath Condensate—A Non-Invasive Approach for Diagnostic Methods in Asthma

2021 ◽  
Vol 10 (12) ◽  
pp. 2697
Author(s):  
Joanna Połomska ◽  
Kamil Bar ◽  
Barbara Sozańska
Keyword(s):  
Airway Inflammation ◽  
Exhaled Breath Condensate ◽  
Clinical Manifestations ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Exhaled Breath ◽  
Invasive Approach ◽  
Non Invasive ◽  
Airway Tone ◽  
Breath Condensate

The pathophysiology of asthma has been intensively studied, but its underlying mechanisms such as airway inflammation, control of airway tone, and bronchial reactivity are still not completely explained. There is an urgent need to implement novel, non-invasive diagnostic tools that can help to investigate local airway inflammation and connect the molecular pathways with the broad spectrum of clinical manifestations of asthma. The new biomarkers of different asthma endotypes could be used to confirm diagnosis, predict asthma exacerbations, or evaluate treatment response. In this paper, we briefly describe the characteristics of exhaled breath condensate (EBC) that is considered to be an interesting source of biomarkers of lung disorders. We look at the composition of EBC, some aspects of the collection procedure, the proposed biomarkers for asthma, and its clinical implications. We also indicate the limitations of the method and potential strategies to standardize the procedure of EBC collection and analytical methods.

scholarly journals Modular Breath Analyzer (MBA): Introduction of a Breath Analyzer Platform Based on an Innovative and Unique, Modular eNose Concept for Breath Diagnostics and Utilization of Calibration Transfer Methods in Breath Analysis Studies

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3776
Author(s):  
Carsten Jaeschke ◽  
Marta Padilla ◽  
Johannes Glöckler ◽  
Inese Polaka ◽  
Martins Leja ◽  
...  
Keyword(s):  
Real Life ◽  
Sensor Arrays ◽  
Breath Analysis ◽  
Exhaled Breath ◽  
Calibration Transfer ◽  
Early Disease Detection ◽  
Exhaled Breath Analysis ◽  
Breath Analyzer ◽  
Analysis Experiment ◽  
Before And After

Exhaled breath analysis for early disease detection may provide a convenient method for painless and non-invasive diagnosis. In this work, a novel, compact and easy-to-use breath analyzer platform with a modular sensing chamber and direct breath sampling unit is presented. The developed analyzer system comprises a compact, low volume, temperature-controlled sensing chamber in three modules that can host any type of resistive gas sensor arrays. Furthermore, in this study three modular breath analyzers are explicitly tested for reproducibility in a real-life breath analysis experiment with several calibration transfer (CT) techniques using transfer samples from the experiment. The experiment consists of classifying breath samples from 15 subjects before and after eating a specific meal using three instruments. We investigate the possibility to transfer calibration models across instruments using transfer samples from the experiment under study, since representative samples of human breath at some conditions are difficult to simulate in a laboratory. For example, exhaled breath from subjects suffering from a disease for which the biomarkers are mostly unknown. Results show that many transfer samples of all the classes under study (in our case meal/no meal) are needed, although some CT methods present reasonably good results with only one class.

scholarly journals Circulating miRNAs Related to Epithelial–Mesenchymal Transitions (EMT) as the New Molecular Markers in Endometriosis

2021 ◽  
Vol 43 (2) ◽  
pp. 900-916
Author(s):  
Anna Zubrzycka ◽  
Monika Migdalska-Sęk ◽  
Sławomir Jędrzejczyk ◽  
Ewa Brzeziańska-Lasota
Keyword(s):  
Molecular Mechanisms ◽  
Immune Cell ◽  
Epithelial Mesenchymal Transition ◽  
Clinical Symptoms ◽  
Diagnostic Tools ◽  
Endometrial Stromal Cells ◽  
Disease Etiology ◽  
Diagnostic Biomarkers ◽  
Mesenchymal Transition ◽  
Non Invasive

Endometriosis is a chronic gynecological disease defined by the presence of endometrial-like tissue found outside the uterus, most commonly in the peritoneal cavity. Endometriosis lesions are heterogenous but usually contain endometrial stromal cells and epithelial glands, immune cell infiltrates and are vascularized and innervated by nerves. The complex etiopathogenesis and heterogenity of the clinical symptoms, as well as the lack of a specific non-invasive diagnostic biomarkers, underline the need for more advanced diagnostic tools. Unfortunately, the contribution of environmental, hormonal and immunological factors in the disease etiology is insufficient, and the contribution of genetic/epigenetic factors is still fragmentary. Therefore, there is a need for more focused study on the molecular mechanisms of endometriosis and non-invasive diagnostic monitoring systems. MicroRNAs (miRNAs) demonstrate high stability and tissue specificity and play a significant role in modulating a range of molecular pathways, and hence may be suitable diagnostic biomarkers for the origin and development of endometriosis. Of these, the most frequently studied are those related to endometriosis, including those involved in epithelial–mesenchymal transition (EMT), whose expression is altered in plasma or endometriotic lesion biopsies; however, the results are ambiguous. Specific miRNAs expressed in endometriosis may serve as diagnostics markers with prognostic value, and they have been proposed as molecular targets for treatment. The aim of this review is to present selected miRNAs associated with EMT known to have experimentally confirmed significance, and discuss their utility as biomarkers in endometriosis.

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