Determination of nutritional and functional principles of the jujube pulp fruit, ecotype Zfisef from northeastern of Algeria

2015 ◽  
Vol 5 (3) ◽  
pp. 84-93
Author(s):  
Insaf Fatima Zohra Mansour ◽  
Mokhtaria Yasmina Boufadi ◽  
Fatima Zohra Elkadi ◽  
Amina Imène Benali ◽  
Mohammed Benali

  The aim of the present work was to valorize the exotic fruit of jujube, Zfisef ecotype, growing in the western region of Algeria and highly appreciated by the population. The various analyzes of primary metabolites showed an interesting nutritional profile. The fruit pulp of jujube had a dry matter content of 81±0.21%. The amount of ash (6.1±0.16%) was high and demonstrated a wealth of minerals. Total sugars represented more than 23.20±1.85% while proteins and lipids represented only 2.88±0.32 SAB equivalent and 0.28±0.03% respectively. Vitamin C from the pulp of the fruit of the jujube tree was 194±4.36 mg/100 g of substrate and appeared significant compared to other varieties of other biotope and to fruit ripening stages. The fruit pulp of jujube (50g) gave an ethanol extract of 3.78±0.31%. The latter allowed us to determine secondary metabolites, in this case the total polyphenols (7.80±0.40 mg EAG/g) flavonoids (25.58±1.96 mg EQ/g) gallic tannins (0.15±0.025 μg EAG/100g) catechin tannins (3.48±0.28 μg ECT/mg) total anthocyanins (4.81±0.44 mg ECy3g/100g) and total carotenoids (5.02±0.19 mg E b-carotene/100g). These biologically active molecules conferred to the product good DPPH antioxidant activity (56.60±3.99% with PFJ extract concentration of 0.25±0.062 mg/ml). Concentration of product required for 50% inhibition was 0.22mg/ml. Iron reduction power of our jujube fruit extract exhibited a good antioxidant activity compared to ascorbic acid. Also the presence of many phenolic compounds determined by LC-MS/MS with var-ied identities and antioxidant characters generate interest in making this product a functional food.

Author(s):  
Н.Б. ЕРЕМЕЕВА ◽  
Н.В. МАКАРОВА

Подобраны оптимальные параметры для экстракции плодов и ягод черной смородины, малины, вишни, черноплодной рябины при ультразвуковом излучении 35 кГц с целью получения плодово-ягодных экстрактов с высокой антиоксидантной активностью. На основании экспериментальных данных выбраны технологические режимы процесса: экстрагирование сырья 75%-м этиловым спиртом при соотношении сырье : растворитель 1 : 10, температуре (40 ± 5)°C в течение 90 мин. Экстракты концентрируют под вакуумом до содержания сухих веществ 65%. Дана технологическая схема получения плодово-ягодных экстрактов. Полученные экстракты обладают высокой антиоксидантной активностью, содержат большое количество фенольных веществ, флавоноидов и антоцианов; по органолептическим, физико-химическим и микробиологическим показателям соответствуют нормативной документации. The optimal parameters for extraction of fruits and berries of fruits and berries of black currant, raspberry, cherry, chokeberry with ultrasonic radiation of 35 kHz in order to obtain fruit and berry extracts with high antioxidant activity are selected. Based on the experimental data, the technological modes of the process are selected: extraction of raw materials with 75% ethyl alcohol at a ratio of raw materials: solvent 1: 10, temperature (40 ± 5)°C for 90 min. The extracts are concentrated under vacuum to a dry matter content of 65%. Technological scheme of fruit and berry extracts is given. The obtained extracts have a high antioxidant activity, contain a large amount of phenolic substances, flavonoids and anthocyanins; organoleptic, physical and chemical and microbiological parameters correspond to the normative documentation.


2021 ◽  
Vol 39 (3) ◽  
pp. 299-304
Author(s):  
Lucimeire Pilon ◽  
Jaqueline S Guedes ◽  
Bruna S Bitencourt ◽  
Raphael Augusto de C Melo ◽  
Larissa PC Vendrame ◽  
...  

ABSTRACT Sweetpotato (Ipomoea batatas) is a root crop grown in many countries. This tuberous root is a source of energy, nutrients, and phytochemicals. In this study, bioactive compounds and physical and physicochemical qualities of sweetpotato genotypes were evaluated. Eight new genotypes of sweetpotato produced by Embrapa Hortaliças (orange-fleshed: MD09026-OF and MD09024-OF; cream-fleshed: MD09011-CF, MD09004-CF, MD10039-CF, and MD10004-CF; yellow-fleshed: MD09017-YF and MD12002-YF) and two cultivars used as controls (Beauregard and Brazlândia Roxa) were evaluated for color, soluble solids, dry matter, phenolic compounds, total carotenoids and β-carotene. Hue angles differed even between those sweetpotatoes with the same flesh color. The orange-fleshed genotypes MD09024-OF, MD09026-OF, and Beauregard, had the lowest L*, showing to be darker than the others. These sweetpotatoes also had the brightest flesh colors with higher C*. The orange-fleshed genotypes MD09026-OF and MD09024-OF were sweeter (10.55oBrix and 9.23oBrix) than Beauregard (5.12oBrix). Brazlândia Roxa had the highest dry matter content (38.05%), followed by the genotypes MD10004-CF, MD09017-YF, MD09026-OF MD10039-CF, and MD09011-CF, which showed similarity, ranging from 32.33% to 29.12%. The highest contents of total carotenoids were found for the orange-fleshed genotypes MD09026-OF (80.06 mg g-1) and MD09024-OF (70.56 mg g-1) and Beauregard (73.12 mg g-1). These same genotypes showed the highest total phenolic compounds (0.815 mg g-1 and 0.686 mg g-1, respectively). MD09026-OF showed the highest content of β-carotene (46.47 mg g-1). MD09026-OF was the most prominent genotype among those evaluated, as it showed the highest total carotenoid, β-carotene, phenolic compounds, and soluble solids content, in addition to a high dry matter content.


2020 ◽  
Vol 10 (15) ◽  
pp. 5254
Author(s):  
Marius Lasinskas ◽  
Elvyra Jariene ◽  
Nijole Vaitkeviciene ◽  
Jurgita Kulaitiene ◽  
Katarzyna Najman ◽  
...  

The demand for organic production is increasing worldwide. The willowherb, grown in an organic way, contributes greatly to the idea of a healthier society and clean land. Willowherb is widespread in the world and has high polyphenols, carotenoids, and antioxidant properties. The purpose of this work was to investigate the influence of solid-phase fermentation (SPF) under different conditions on the variation of polyphenols and carotenoids in the organic leaves of willowherb. The leaves were fermented for different periods of time: 24, 48, and 72 h; and in aerobic and anaerobic conditions. The evaluation of polyphenols and carotenoids was completed using high-performance liquid chromatography (HPLC), and antioxidant activity was measured with spectrophotometric method. Hierarchical cluster analysis was used to describe differences in biologically active compounds between willowherb samples. The experiment showed that the highest quantities of total phenolic acids and flavonoids were determined after 24 h under aerobic SPF, but the amountof total carotenoids was higher after 72 h anaerobic SPF, compared to control. Not-fermented willowherb leaves had a lower antioxidant activity. compared to fermented leaves. In conclusion, SPF can be used to change polyphenol and carotenoid quantities in organic leaves of willowherb.


Author(s):  
С.О. СЕМЕНИХИН ◽  
О.В. ФЕДОСЕЕВА ◽  
М.В. БАБАКИНА ◽  
Н.М. ДАИШЕВА

Проведены исследования обработки растворов свекловичной мелассы 4 штаммами микроорганизмов с целью обогащения растворов жирами. Выявлено влияние начального значения рН, а также оптимального содержания сухих веществ в растворах мелассы, обеспечивающие эффективную жизнедеятельность микроорганизмов с синтезом биологически активных веществ. Установлено, что штаммы R. glutinis Y-332, C. curvatus Y-2236 и U. isabellina F-526 в растворах мелассы с начальным значением рН 8,5 синтезировали жиры в количестве 0,015 0,017 и 0,007 г/г сахаров соответственно штамм R. toruloides Y-334 в растворе мелассы с начальным значением рН 7,0 синтезировал жиры в количестве 0,004 г/г сахаров. Впервые получены экспериментальные данные, позволившие оценить эффективность микробиологического синтеза жиров в растворах мелассы без дополнительного внесения биогенных веществ, а также без поддержания оптимальной рН среды 4 видов микроорганизмов. Обосновано, что штаммы R. glutinis Y-332 и C. curvatus Y-2236 являются наиболее перспективными для обогащения растворов свекловичной мелассы жирами. Research aimed at treatment of beet molasses solutions with 4 strains of microorganisms in order to enrich the solutions with fats has beed established. The influence of the initial pH value, as well as the optimal dry matter content in molasses solutions, which ensure the effective vital activity of microorganisms with the synthesis of biologically active substances, is revealed. It was established that the strains of R. glutinis Y-332, C. curvatus Y-2236 and U. isabellina F-526 in molasses solutions with an initial pH of 8,5 synthesize fats in an amount of 0,015 0,017 and 0,007 g/g of sugars, respectively and the strain of R. toruloides Y-334 in molasses solution with an initial pH value of 7,0 synthesizes fats in an amount of 0,004 g/g of sugars. For the first time, it was obtained the experimental data that made possible to evaluate effectiveness of microbiological synthesis of fats in molasses solutions without nutrient addition, as well as without maintaining the optimal pH of the environment for 4 types of microorganisms. It is proved that R. glutinis strain Y-332 and C. curvatus strain Y-2236 are the most promising strains for enriching beet molasses solutions with fats.


Inventions ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 59
Author(s):  
Diana Roman ◽  
Nina Nicoleta Condurache (Lazăr) ◽  
Iuliana Aprodu ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
...  

Sea buckthorn (Hippophae rhamnoides L.) represents a valuable source of biologically active compounds such as carotenoids and polyphenols. High amounts of these substances are found in its fruits, bark, and leaves. However, their bioavailability is limited and must be increased in order to benefit from the properties they exert. Therefore, the purpose of this study was to increase the stability and bioavailability of sea buckthorn fruit’s bioactives. The sea buckthorn’s bioactive compounds were extracted with a solvent combination between glacial acetic acid, acetone, and water on one side and water only on the other side. Afterward, the phytochemicals from the extracts were encapsulated using the coacervation technique, followed by freeze-drying in order to obtain stable powders. The powders were characterized in terms of antioxidant activity, total carotenoids, β-carotene, lycopene, total polyphenol, and total flavonoid content, color, structure, and morphology. The phytochemical stability of the powders and their antioxidant activity was assessed during 270 days of storage at 4 °C. Moreover, the bioavailability of phytochemicals was measured during in vitro simulated digestibility. Our findings provide insights to promote carotenoids and polyphenols from sea buckthorn as bioactive ingredients with multiple purposes.


Author(s):  
THANIARASU R ◽  
LOGESHWARI M

Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.


2018 ◽  
Vol 40 (6) ◽  
Author(s):  
Lisiane Pintanela Vergara ◽  
Gabriela Niemeyer Reissig ◽  
Rodrigo Cezar Franzon ◽  
Rosane da Silva Rodrigues ◽  
Josiane Freitas Chim

Abstract Brazil presents a great variety of native fruits, which have aroused commercial interest, such as the strawberry guava. In this sense, many researches have looked for alternatives that allow postharvest availability of fruits, considering that perishability is a limiting factor for its commercialization. The freezing process is an important alternative in the sense of making these fruits available for longer on the market. The use of L-ascorbic acid is a potent protective agent in the stability of bioactive compounds in fruit pulps. The objective of this study was to evaluate potentially bioactive compounds present in red and yellow strawberry guava pulps added with L-ascorbic acid (1% m / m) and verify its effect on retention of these compounds and in its antioxidant activity during the frozen storage (-20 ± 2ºC). In the pulps were analyzed: antioxidant activity by the capture of the DPPH° and ABTS° radicals, phenols, anthocyanins and total carotenoids. L-ascorbic acid positively influences the retention of bioactive compounds (total phenols, total anthocyanins and total carotenoids) in red and yellow strawberry guava pulps during 120 days of frozen storage (-20 ± 2 ºC).


2021 ◽  
pp. 87-95
Author(s):  
I. V. Gashkova ◽  
A. E. Solovieva ◽  
A. B. Kurina

Relevance. The presence of valuable biologically active substances, carbohydrates, organic acids and others in the eggplant fruits makes the culture one of the vegetables with the highest antioxidant activity. The VIR collection of eggplant includes 830 accessions from 70 countries of the world. The study of new acquisitions in the VIR collection presupposes a comprehensive assessment of the received material according to morphological, biological and economically valuable characteristics. The main objective of the study was to assess the variability of the biochemical parameters of egg-plant fruits in technical ripeness; as well as determination of the best accessions by the chemical composition of fruits and the content of biologically active substances.Materials and methods. The study of 19 accessions of eggplant accessions was carried out in 2020 in a winter greenhouse in Pushkin and Pavlovsk Laboratories of VIR (St. Petersburg). The morphological description of the accessions and the assessment for biological and economically valuable traits were carried out in accordance with the methodological guidelines and the VIR classifier. Biochemical analysis was carried out in the Department of Biochemistry and Molecular Biology of VIR in the phase of technical ripeness of fruits in terms of: dry matter content, sugars, total acidity, ascorbic acid, pigments and anthocyanins.Results. As a result of this study, the amplitude of variability in the content of dry matter (6.44- 8.68%), sugars (1.78-3.72%), ascorbic acid (5.92-21.08 mg/100 g), titrated acidity (0.10-0.31%), chlorophylls (0.52-15.13 mg/100 g), carotenoids (1.19-6.99 mg/100 g), β-carotene (0.11-0.52 mg/100 g) and anthocyanins (12.94-1031.40 mg/100 g) in eggplant fruits. Accessions with a high content of biologically active substances in fruits in technical ripeness were identified: Russian hybrids Bourgeois F1, Azhur F1; local accessions from Armenia: k-3156, k-3159, k-3161.


Author(s):  
Edita Juknevičienė ◽  
Honorata Danilčenko ◽  
Elvyra Jarienė ◽  
Vilma Živatkauskienė ◽  
Johanna Zeise ◽  
...  

Abstract Background Food quality of agricultural crops depends on environmental conditions, production system and cultivation method. A plant-based nutrition with food rich in vitamins, minerals and secondary plant compounds with antioxidative properties promotes human health. This investigation was inspired by an increasing global issue on how to improve product quality while using alternative preparations. The main aim of a 3-year study was to investigate the influence of fermented manure and silica products on yield and nutritive components in peel, fruit and seeds of three pumpkin cultivars. In four replicates as block design, the effects of individually as well as combined application of biodynamic horn manure and horn silica preparation were compared to a control variant. Results Horn manure application significantly increased total and marketable yield. Marketable yield, contents of macroelements, total carotenoids, single carotenoids (lutein + zeaxanthin, lycopene, ß-carotene) and antioxidants (catechins, total phenols, leuco-anthocyanins) were significantly increased by horn silica use. The combination of both biodynamic preparations had a significantly increasing effect on total and marketable yield, net photosynthetic productivity, dry matter content and total and single carotenoid contents (lutein + zeaxanthin, lycopene, ß-carotene). Conclusions The pumpkin trial results indicated a general growth-promoting effect by horn manure, a quality-enhancing effect by horn silica and a compensatory effect through both preparations on a high qualitative level. In accordance with other investigations, these effects did not occur in the same way in all plant species. Therefore, the effects of the biodynamic preparations should be tested in further trials on a plant species-specific basis. Graphic abstract


2021 ◽  
Vol 332 ◽  
pp. 07002
Author(s):  
Tri Nhut Pham ◽  
Tien Dung Le ◽  
Van Tuyen Nguyen ◽  
Trong Doan Phan ◽  
Duc Quang Thach ◽  
...  

The purpose of this study was to examine the phytochemical content and antioxidant activity of Coptosapelta flavescens Korth root extract obtained from various places. Phytochemical screening, antioxidant activity testing using DPPH radical reduction, color reaction (ABTS), iron reduction (FRAP), reduction capacity technique (RP), and total antioxidant capacity (TAC). The phytochemical tests revealed that C. flavescens water and ethanol extract included alkaloids, coumarins, terpenoids, tannins, and flavonoids. The antioxidant activity results revealed that raw material samples gathered directly in the wild in Ninh Son district (Ninh Thuan province, Vietnam) were more effective than those purchased at the local market with EC50 times value. DPPH, ABTS, FRAP, RP, and TAC techniques yielded 56.02 µg/ml, 54.12 µg/ml, 15.9 µg/ml, 44.85 µg/ml, and 110.94 µg/ml, respectively. The antioxidant impact of raw material samples obtained in Bac Ai district (Ninh Son province, Vietnam) was lower but overall better than that of samples gathered at the local market.


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