scholarly journals Evaluation of various barley processing methods on rumen microbial population, histomorphometry, and fermentation characteristics in fattening lamb

2020 ◽  
Vol 44 (5) ◽  
pp. 961-967
Author(s):  
Mohammad Hossein ZANGNE ◽  
Masihollah FOROUZMAND ◽  
Mokhtar KHAJAVI ◽  
Shima HOSSEINIFAR ◽  
Reza NAGHIHA

Barley deserves a top place in the farm for feeding livestock. It is irreplaceable by any other grain in sheep diets for producing capacious rumen microbial yields. The present study was conducted to investigatethe effect of different methods of barley processing on a number of microbes, fermentation parameters, and the rumen tissue characteristics of fattening lambs. For this purpose, 20 fattening male lambs with a mean weight of 25 ± 1.1 kg were tested for 80 daysbasedon a completely randomized design with four treatments (GRB: ground barley, SRB: steam-rolled barley, GEB: germinated barley, SOB: soaked barley) and five replicates. Three lambs from each treatment were slaughtered and sampling was done on the rumen liquid and tissue to determine their pH and volatile fatty acid (VFA), amylolytic bacteria, proteolytic, cellulolytic, heterophilic, and protozoa contents. The findings showed that the number of cellulolytic bacteria in ground barley treatment was lower than that of soaked barley treatment. In addition, there existed fewer heterophilic bacteria in germinated barley groups compared with other treatments. The lactic acid level in the GRB was increased compared to that in SRB and GEB treatments (P < 0.05). The GRB further reduced the thickness of the rumen wall in comparison with SRB. In general, replacing GRB with SOB improved certain thickness of the epithelial tissue and cellulolytic bacteria of the rumen.

2013 ◽  
Vol 15 (2) ◽  
pp. 64 ◽  
Author(s):  
Khabib Khasan Alfaridhi ◽  
Arina Tri Lunggani ◽  
Endang Kusdiyantini

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus


2015 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Fahrizal Hazra

<p>ABSTRACT</p><p>Snake fruit [Salacca zalacca (Gaertner) Voss] Pondoh is one of important fruits in Indonesia. The objective of the study was to determine the  influence of some selected potential microbes such as Phosphate Solubilizing Bacteria, Cellulolytic Bacteria, and  N2-fixing Bacteria (Azospirillum)  ongrowth of seedling  of  snake  fruit  Pondoh.  A  study  was  conducted  in  the nursery. The  experiment used  a  completely  randomized  design  with  treatments namely:  K0  (without  inoculum);  A1 (Azospirillum inoculum dose 5 ml), A2 (inoculum 25 ml); F1 (BPF inoculum dose 5 ml), F2 (dose 25 ml); S1; BPS inoculums (dose 5 ml), S2 (dose 25 ml); C1 (mixed bacterial inoculum A+F+S dose 5 ml), and C2 (A+F+S dose 25 ml). Plant height, P, C/N soil ratio, N were measured in 10 months for observing the impact of treatments. Results showed that the effect of inoculation of A2 to the amount of  N-total  (0.99%)  was significantly  different  compared  to  soil  with  K0;  and  not significantly different  from  the  amount  of  N-total  inoculation  A1,  F1,  F2, S1,  S2,  C1,  and  C2.  F2  increased availability of P (20.79 ppm) in the soil higher than  K0, A1, F1; and not significantly different from A2, S1, S2, C1, and C2.  K0 treatment gave  C/N ratio of soil significantly different from A1, A2, F1, F2, S1, S2, C1 and C2; BPS which showed activity in overhauling C-organic in the soil. Inoculation A2  and F2  increased  plant  height  15.48% and 9.31%  higher  than  the  control.  Inoculation  of  C2 differed  significantly  in improving  the  fresh  weight  compared  to  control;  and  not significantly different  from  A1,  A2,  F1,  F2,  S1,  S2,  and  C1  and  able  to increase  the  dry  weight  of  71.12% compared to the control.</p><p>Keywords: Azospirillum, Cellulolytic Bacteria, Phosphate Solubilizing Bacteria, Snake Fruit</p><p> </p><p>ABSTRAK</p><p>Salak Pondoh [Salacca zalacca (Gaertner) Voss] merupakan tanaman buah bernilai tinggi dan diminati  masyarakat.  Budidaya  salak  diawali  dengan pembibitan.  Penelitian  ini  bertujuan  menguji pengaruh  bakteri  potensial hasil  isolasi  dan  seleksi  dari  bakteri  pelarut  fosfat  (BPF), bakteri perombak  selulosa  (BPS),  dan  bakteri  pemfiksasi  N2  (Azospirillum) dalam  pertumbuhan  bibit tanaman  salak pondoh. Penelitian ini menggunakan rancangan acak lengkap satu faktor yang terdiri atas: K0 (tanpa inokulum/Kontrol);  A1  (inokulum  Azospirillum  5  ml), A2 (25  ml inokulum);  F1 (inokulum BPF dosis 5 ml), F2 (dosis 25 ml); S1 (inokulum BPS dosis 5  ml), S2 (dosis 25 ml); C1 (inokulum campuran A+F+S dosis 5 ml), dan C2 (dosis 25 ml).  Pengamatan dilakukan selama 10 minggu  sampai  bibit salak  Pondoh  memiliki dua daun.  Hasil penelitian menunjukkan  inokulasi A2dan S2  nyata  jumlah N-total (0.99%) di dalam tanah dibandingkan  dengan K0; tetapi  tidak berbeda nyata dibandingkan dengan perlakuan lain. Inokulasi F2 nyata meningkatkan P tersedia (20.79 ppm) di dalam tanah dibandingkan dengan K0, A1, F1; dan tidak berbeda nyata dengan A2, S1, S2, C1, dan C2. Induksi dengan seluruh isolat nyata terhadap C/N rasio tanah; yang menunjukkan adanya aktivitas BPS dalam  merombak C-organik  di dalam tanah.  Inokulasi  A2  dan  F2  mampu meningkatkan tinggi tanaman 9.31% dan 15.48% lebih  besar  dibandingkan kontrol. Inokulasi C2 nyata meningkatkan bobot basah bibit salak pondoh dibandingkan kontrol; tetapi tidak berbeda nyata dengan perlakuan lain serta mampu meningkatkan bobot kering 71.12% dibandingkan kontrol.</p><p>Kata kunci: Azospirillum, bakteri pelarut fosfat, bakteri perombak selulosa, Salak Pondoh</p>


2019 ◽  
pp. 107-111
Author(s):  
Alagbe J. O. ◽  
Olanrewaju Abiodun ◽  
Adewemimo A. ◽  
Tanimomo Babatunde Kayode

The experiment was conducted to evaluate the carcass, caeca microbial parameters of broilers fed different levels of mixed lemon grass and garlic extract (CLGE). Five hundred broiler chickens (Ross 308) were allotted to five treatments with five replicate consisting of 20 birds each in a completely randomized design (CRD). The first group T1 was given 0.025g/litre Neomycin in water while T2, T3, T4 and T5 were given CLGE at levels 3.0ml/litre, 6.0ml/litre, 9.0ml/litre and 12.0 ml/ litre of water respectively. The experiment lasted for four weeks each for the starter and finisher respectively. Results on dressing percentages revealed that birds given 12.0 ml/l CLGE was highest (76.87%) followed by those in T4 (76.54 %), T3 (75.61 %), T2 (75.00%) and T1 (70.01%). Significant influences (P<0.05) were also observed in the relative organ weight (liver, kidney, spleen, heart, pancreas, gizzard and proventriculus). There were also significant differences (P<0.05) in the caeca microbial population of E.coli and lactobacillus count as well as the antibody titer against Newcastle and gumboro disease. Birds in T5 had the lowest E.coli count (9.00 cfu/g) when compared to T1 (22.19 cfu/g) with the highest proportion of the bacteria. It was concluded that CLGE could be given to broilers at 12.0ml/liter without any negative effect on the health and can be used to effectively replace antibiotics.


2019 ◽  
Vol 7 (2) ◽  
pp. 1-9
Author(s):  
Nurzainah Ginting ◽  
Hasnudi ◽  
Yunilas ◽  
M.Adanan Purba

This research was conducted in Samosir Island, North Sumatera Province, Indonesiawhich has Inceptisol soil. As this type of soil is generally unfertile, since hundreds years ago,local people added ruminant faeces before planting. This research objective was to prove thatapplication of biogas slurry on growing phase of Turi (Sesbania grandiflora) plant improvedmicrobial population on soil thus plant quality. This research was conducted from May untilSeptember 2018. Research design was Completely Randomized Design (CRD) withtreatments slurry dosages which were P0: without slurry, P1: 10/ha/year and P2: 20/ha/year.Parameters were chemical composition of slurry, microbial type and population, plant height,total leaf, stem diameter, number of branch and leaf size. The results showed that P0 had nosignificant effect on every parameters while P1 and P2 improved microbial type andpopulation, plant height, total leaf, stem diameter, number of branch and leaf size (P <0.05).There was an increasing of microbial population in line with the increasing of slurry dosage.P2 contained the highest population of bacteria or fungal (F <0.05) compared to P0 or P1.


2019 ◽  
Vol 68 (264) ◽  
pp. 576-581
Author(s):  
L. Antunes Stella ◽  
V. Rosa Prates ◽  
A. Zubieta ◽  
C. Bayer ◽  
J.O. Jardim Barcellos

The objective of this study to evaluate the effect of secondary plant compounds present in essential oils in replacement of monensin on in vitro ruminal fermentation parameters. It was adopted a completely randomized design with nine treatments and four replicates. The treatments were: control (CON), monensin (MON), garlic oil (ALH), cinnamon oil (CAN), clove oil (CRA), mint oil (HOR), juniper oil (JUN), bitter orange oil (LAR), and melaleuca oil (MEL). The in vitro gas technique was used to record total gas production at 4, 8, 12 and 24 h after incubation. MON, CAN and CRA increased gas production Only the garlic and cinnamon treatments reduced the digestibility of organic matter in 20 and 26% in relation to the control treatment. Methane production reduced (P


2018 ◽  
Vol 9 (2) ◽  
pp. 119
Author(s):  
Eli Sahara ◽  
Tuti Widjastuti ◽  
Rostita L Balia ◽  
Abun Abun

ABSTRAKKondisi ekologis saluran pencernaan  kondusif berpengaruh terhadap kelancaran metabolisme tubuh.  Keseimbangan mikroflora hidup pada saluran pencernaan itik sangat membantu kelancaran pencernaan nutrien ransum menjadi zat yang lebih sederhana.  Kitosan merupakan jenis serat hewan yang sangat cocok untuk menunjang kehidupan bakteri jenis menguntungkan dalam saluran cerna.  Tujuan penelitian adalah melihat perkembangan mikroflora saluran pencernaan dengan pemberian kitosan.  Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan  4 perlakuan dan 5 ulangan.  Masing-masing ulangan terdiri dari 2 ekor itik sebagai satuan percobaan.  Perlakuan adalah R0 = 0% kitosan, R1 = 0,5% kitosan, R2 = 2% kitosan dan R3 = 2,5% kitosan. Peubah yang diukur adalah jumlah total populasi mikroba, jumlah Salmonela dan jumlah E coli saluran pencernaan itik.  Hasil penelitian menunjukkan bahwa pemberian kitosan 0,5% dan 2% memberikan peningkatan  rataan jumlah total mikroba (TPC) pada saluran cerna.  Kitosan juga  menurunkan jumlah E coli saluran cerna serta kandugan Salmonella sp negative(<10 2  ) Kata kunci: Kitosan, mikroflora, saluran cerna, itik Tegal  ABSTRACT Conducive ecological conditions of the digestive tract affect the smooth metabolism of the body. The balance of living microflora in the digestive tract of ducks greatly helps the smooth digestion of nutrients into simpler substances. Chitosan is a type of animal fiber that is potential to support the life of beneficial type bacteria in the gastrointestinal tract. The aim of this research is to see the development of microflora of gastrointestinal tract by giving chitosan. The study used Completely Randomized Design (RAL) with 4 treatments and 5 replications. Each replication consists of 2 ducks as experimental units. The treatment was R0 = 0% chitosan, R1 = 0,5% chitosan, R2 = 2% chitosan and R3 = 2.5% chitosan. The measured variables were total microbial population, number of Salmonella and amount of E coli of duck digestive tract. The results showed that giving of chitosan 0,5% and 2% give increase of total amount of microbe (TPC) in gastrointestinal tract. Chitosan also decreases the amount of E coli gastrointestinal tract and Salmonella sp negative (<102) Keywords: chitosan, microflora, gastrointestinal tract, Tegal duck


2018 ◽  
Vol 70 (5) ◽  
pp. 1586-1594 ◽  
Author(s):  
L.O. Rosa ◽  
O.G. Pereira ◽  
K.G. Ribeiro ◽  
S.C. Valadares Filho ◽  
P.R. Cecon

ABSTRACT Fermentation profile and microbial population were assessed in soybean silages without any additive (control), with inoculant (I), with I + powdered molasses (I+M), and with powdered molasses only (M). Soybean plants were harvested at the R6 stage and ensiled in 2kg-capacity laboratory silos. The additives were added to the natural matter base of silages. The assessed fermentation periods were 1, 3, 7, 14, 28, and 56 days. A 4 × 6 factorial arrangement (4 additives × 6 fermentation periods) in a completely randomized design with 3 replicates was used. Lactic, acetic, and butyric acids concentrations were influenced by additives and periods (P< 0.05). It was observed higher lactic acid values to control silages, on the 56th day. Lower average values of acetic and butyric acids were observed to I+M and M silages. It was observed quadratic effect to pH values with a reduction estimated of 0.5504, 0.5358, 0.6312 and 0.6680 units to pH values to control, I, I+M, and M silages in the first 10 days. A maximum lactic acid bacteria population was observed at the 28th day of fermentation in silages with inoculant. The inoculant and powdered molasses improve the fermentation profile of soybean silages.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyuzan Wahyuzan ◽  
Lukmanul Hakim ◽  
Rahmat Afrizal ◽  
Asmeri Lamona ◽  
Khairuni Khairuni ◽  
...  

Traditional medicine in Indonesia has been known by the public before formal health services using modern medicine. Indonesia has many type of herbal plants which have medicinal properties including dadap (Erythrina orientalis). This study aims to utilized dadap herbal liniment by modifying heating in Vigin Cocanut Oil. The study conducted in the laboratory of Agricultural Product Processing using a Factorial Completely Randomized Design (CRD). The first factor is the addition of 250 ml (M1), 375 ml (m2), and 400 ml (M3) Virgin Cocanut Oil treatment. The second factor is the heating tim of 5 minutes (L1), 10 minutes (L2), and 15 minutes (L3). Observation variable consisted of yield, Specific gravity, and organoleptic test of aroma and colored. The result showed an average yield of 87.70-91.88%. The highest yield was found in the treatment of adding 400 ml Virgin Cocanut Oil and 15 minutes of heating time. The highest type of weight was found in the treatment of addition of 325 ml Virgin Cocanut Oil and 5 minutes heating time. Based on the organoleptic scent test, the everage penalist gives a like answer. Organoleptic colors of reudeuep herbal products is according to SNI number 06-23-2006.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Silaturahmi Silaturahmi ◽  
Zaidiyah Zaidiyah ◽  
Tengku Mia Rahmiati

The use of colorants in the dried noodle manufactures is an effort of product diversification. Besides of being used as a natural coloring agent, this peel extract is also used to improve nutritional value of the expected product. The purpose of this study was to determine the effect of red dragon fruit peel extract on the physical quality of dried noodle.  The study was conducted by using Completely Randomized Design (CRD) method with one factor, namely concentration of red dragon fruit peel extract (N1 = 10 ml, N2 = 15 ml, N3 = 20 ml, N4 = 25 ml, N5 = 30 ml).  The observations of its physical quality consisted of water absorption, solid loss during cooking, and organoleptic tests (aroma and color).  The best quality of dried noodle was obtained by using 30 ml red dragon fruit peel extract (N5) with physical properties, namely DSA levels of 351.92%, solid loss during cooking 4.78%, aroma 3.79 (like) and color 3.89 (like).


2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Erma Ayu Nurcahyani ◽  
Merkuria Karyantina ◽  
Nanik Suhartatik

Ikan asin adalah metode untuk pengawetan makanan menggunakan kombinasi penggaraman dan pengeringan matahari. Di kalangan masyarakat, pembuatan ikan asin banyak menggunakan bahan kimia berbahaya seperti formalin. Penambahan bahan berbahaya mempengaruhi kualitas terutama keamanan. Makanan yang aman didefinisikan sebagai makanan yang terbebas dari cemaran biologi, kimia, mikrobiologi, yang dapat mempengaruhi kesehatan. Karbon aktif diduga bersifat polar yang mampu mengadsorpsi formalin. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian karbon aktif dalam mengurangi cemaran formalin pada “jambal roti”. Rancangan percobaan yang digunakan untuk percobaan yaitu Rancangan Acak Lengkap (RAL) 2 faktorial, dengan faktor pertama yaitu konsentrasi karbon aktif 0, 3, dan 6% serta perlakuan kedua yaitu lama perebusan selama 5, 10, dan 15 menit. Penelitian ini dimulai dari pembuatan “jambal roti” dengan penambahan formalin dan perebusan dengan karbon aktif kemudian dianalisis uji kadar formalin, NaCl, protein, dan air. Hasil penelitian menunjukkan bahwa konsentrasi karbon aktif dan lama perebusan memberikan pengaruh yang signifikan pada kualitas jambal roti. Semakin tinggi konsentrasi karbon aktif dan lama perebusan maka semakin berkurangnya kadar formalin pada jambal roti. Hasil terbaik penelitian ini adalah karbon aktif 6% dan lama perebusan 15 menit. Menghasilkan formalin 3.21 ppm, NaCl 9.40%, protein 42.00%, dan air 30.02%. Kata kunci: Karbon Aktif, Formalin, Jambal Roti, Lama Perebusan. ABSTRACT                 Salted fish was a method for food preservation using combination of salting and sun drying. Among the people, the manufacture of salted fish use hazardous chemical material such as formaldehyde. The addition of hazardous substances affects the quality especially the safety. Safe food define as food that free from biological, chemical, microbiological contaminants that can affect health. Active carbon suspected to be polar which able to adsorb formaldehyde. The purpose of this research was to determine the influence of active carbon in reducing formaldehyde in salted fish “jambal roti”. The research used completely randomized design (CRD) with 2 factors, the first factor was the concentration of active carbon 0, 3, and 6% and the second was the boiling time (for 5, 10, and 15 min). This research was started from making “jambal roti” with the addition of formaldehyde and boiling with active carbon then analyzed for formaldehyde content, NaCl, protein, and moisture. The result showed that the concentration of active carbon and boiling time had a significant effect in quality salted fish “jambal roti”. The higher the concentration of active carbon and boiling time the more formaldehyde in “jambal roti” were removed. The best results of the research was 6% active carbon and 15 min of boiling time. Resulting 3.21 ppm of formaldehyde, 9.40% of NaCl, 42.00% of protein, and 30.02% of moisture. Keywords: Active Carbon, Formaldehyde, Jambal Roti, Boiling Time


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