Intestinal microbiota and oral administration of Enterococcus faecium associated with the growth performance of new-born piglets

2016 ◽  
Vol 7 (4) ◽  
pp. 529-538 ◽  
Author(s):  
Y.B. Wang ◽  
W. Du ◽  
A.K. Fu ◽  
X.P. Zhang ◽  
Y. Huang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Growth Performance ◽  
Intestinal Microbiota ◽  
Relative Abundance ◽  
Enterococcus Faecium ◽  
Control Group ◽  
New Born ◽  
Significant Difference ◽  
Skimmed Milk ◽  
Weaning Piglets

The oral administration of Enterococcus faecium EF1 to new-born suckling and weaning piglets along with their growth performances and intestinal microbiota was investigated in this study. Twenty-four new-born piglets were initially divided into 2 groups. The probiotics group received 2 ml of 10% sterilised skimmed milk by oral gavage supplemented with 6×108 cfu/ml viable E. faecium EF1 at the first, the third and the fifth day after birth, while the control group received 2 ml of 10% sterilised skimmed milk without probiotics at the same time. Results showed that oral administration of E. faecium EF1 was associated with a remarkable increase on the body weight of piglets for both suckling and weaning periods, by 30.73% (P<0.01) and 320.84% (P<0.01), and also decreased the diarrhoea rate, by 43.21% (P<0.05) and 71.42% (P<0.05), respectively. In addition, 454-pyrosequencing analysis revealed that there was no significant difference in the intestinal microbial diversity of the suckling piglets between the two groups; nevertheless, when compared to the control group, the relative abundance of Firmicutes in the probiotics group was substantially augmented, while the relative abundance of Proteobacteria, Bacteroidetes and Fusobacteria diminished. However, results indicated that oral administration of E. faecium EF1 did not have any influence on the relative abundance of Firmicutes in weaning piglets rather than increasing the relative abundance of Bacteroidetes and decreasing the relative abundance of Proteobacteria. Furthermore, at the level of the Firmicutes phylum, the relative abundance of Lactobacillales in the probiotic group increased significantly. These findings suggest that oral administration of E. faecium EF1 to new-born piglets could improve the growth performance and intestinal microbiota of piglets for both suckling and weaning periods.

scholarly journals Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

2020 ◽  
Vol 8 ◽  
Author(s):  
Shuai Chen ◽  
Xin Wu ◽  
Xia Wang ◽  
Yirui Shao ◽  
Qiang Tu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Relative Abundance ◽  
Lactobacillus Reuteri ◽  
Binding Capacity ◽  
Alpha Diversity ◽  
Sequencing Analysis ◽  
Dietary Iron ◽  
Transcript Profile ◽  
Weaning Piglets

Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans.

scholarly journals Effects of Dietary Tributyrin on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3425
Author(s):  
Li Gong ◽  
Gengsheng Xiao ◽  
Liwei Zheng ◽  
Xia Yan ◽  
Qien Qi ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Relative Abundance ◽  
Size Analysis ◽  
Control Group ◽  
Feed Conversion ◽  
Principal Coordinates Analysis ◽  
Linear Discriminant ◽  
Principal Coordinates ◽  
Biochemical Indices

This study aimed to evaluate the effects of tributyrin on growth performance, biochemical indices and intestinal microbiota of yellow-feathered broilers. 360 one-day-old chicks were randomly allocated to three treatments with six replicates of 20 chicks each, including a normal control group (NC), an antibiotic group (PC), and a tributyrin (250 mg/kg) group (TB) for 63 days. The results showed that compared with the control, the feed conversion ratio (FCR) in the TB group decreased during the d22 to d42 (p < 0.05) and overall, the final weight and FCR of broilers tended to increase and decrease, respectively. Moreover, the TB group showed the highest creatine concentrations at the entire period (p < 0.05). TB treatment increased the Bacteroidetes relative abundance and decreased Firmicutes. Principal coordinates analysis yielded clear clustering of the three groups. Linear discriminant analysis effect size analysis found seven differentially abundant taxa in the TB group, including several members of Bacteroidedetes. The relative abundance of Eisenbergiella, Phascolarctobacterium, Megasphaera and Intestinimonas increased in tributyrin-treated broilers. Spearman correlation analysis identified a correlation between Eisenbergiella abundance and overall feed efficiency. These results demonstrated that tributyrin could improve the growth performance by modulating blood biochemical indices and the cecal microflora composition of broilers.

Combined administration of bacteriocin- -producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits

2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  
Keyword(s):  
Growth Performance ◽  
Enterococcus Faecium ◽  
Average Daily Gain ◽  
Probiotic Strain ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Feed Conversion ◽  
Eleutherococcus Senticosus ◽  
Commercial Diet ◽  
And Control

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.

scholarly journals Comparison of Oral Hygiene Status and Relative Abundance of Periodontal Pathogens Between Individuals With and Without Intellectual Disabilities: a Cross-Sectional Study

2020 ◽  
Author(s):  
Yuki Oda ◽  
Chiaki Furutani ◽  
Reo Kawano ◽  
Jumpei Murakami ◽  
Yuika Mizota ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Relative Abundance ◽  
Outpatient Clinic ◽  
High Throughput Sequencing ◽  
Analysis Of Covariance ◽  
University Hospital ◽  
Permanent Teeth ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Significant Difference

Abstract Background: Studies have reported a high prevalence of periodontal disease in individuals with intellectual disability (ID). The aim of this study was to compare the relative abundance of periodontal pathogens (red, orange, yellow, purple, and green complexes) between individuals with ID and healthy controls.Methods: Of the 31 subjects enrolled in this study, 16 with severe ID were selected from the outpatient clinic of the Special Care Dentistry of Hiroshima University Hospital, and 14 healthy subjects were selected from the outpatient clinic of another department at the same hospital. Dental plaque was sampled after oral examination. Decayed, missing, and filled permanent teeth (DMFT) counts were obtained and periodontal measurements were taken using the papillary-marginal-attached (PMA) index, plaque index (PI), gingival index (GI), and probing depth (PD). Gene sequencing (16S rRNA) was performed for each sample using next-generation high-throughput sequencing methods. The relative abundance of the periodontal pathogens and the clinical parameters were compared. An unpaired t-test was used to compare the oral health status in both groups and analysis of covariance was performed to compare the relative abundance of each pathogen.Results: No statistically significant difference in DMFT was observed between the two groups. However, significant differences in the median PMA index, PI, and GI were noted between the groups (P < 0.0001). In addition, the mean PD in the ID group was significantly higher than that in the control group (P < 0.0001). The relative abundances of Tannerella spp. and Treponema spp. were significantly higher in the ID group when compared to the control group at the genus level (P = 0.0383 and 0.0432, respectively); alternatively, the relative abundance of Porphyromonas spp. was significantly lower in the ID group (P < 0.0001).Conclusions: The PMA index, PI, GI, and PD were significantly lower in the ID group than in the control group. On the other hand, no significant difference in DMFT was observed between the two groups. Furthermore, our findings indicate that Tannerella forsythia might be more closely associated with periodontal disease than Porphyromonas gingivalis in individuals with ID.

scholarly journals Effects of Acanthopanax senticosus supplementation on innate immunity and changes of related immune factors in healthy mice

2020 ◽  
pp. 175342592095520
Author(s):  
YunQiang Zhang ◽  
YunLu Zhang ◽  
ZiKui Liu
Keyword(s):  
Innate Immunity ◽  
Growth Performance ◽  
Innate Immune ◽  
Secretory Iga ◽  
Mouse Serum ◽  
Control Group ◽  
Dependent Manner ◽  
Acanthopanax Senticosus ◽  
Significant Difference ◽  
Immune Factors

Modern scientific research has shown that Acanthopanax senticosus (AS) can regulate the innate immunity of healthy animals, thus affecting the health of animals. However, there are few systematic reports on the changes of innate immune indices of healthy animals after consuming AS. The purpose of this project was to study the effect on healthy mice’s innate immunity and changes of related immune factors induced by feeding AS root powder supplementation. The results showed that the killing rate of natural cells increased in a dose-dependent manner in a certain time period. Compared to the control group, the treatment groups (T1, T2 and T3) improved significantly in the innate immune index (lysozyme, β-defensin-2 and duodenal secretory IgA (SIgA) to varying degrees) and induced corresponding changes of immune factors at certain time periods. The correlation between SIgA and IFN-γ in mouse serum was enhanced, and the higher the concentration of AS in the diet, the stronger the correlation was. However, there was no significant difference in growth performance among groups. It is proved that AS supplementation can enhance innate immunity and change several relevant immune factors and cells of healthy mice without affecting growth performance.

scholarly journals Maternal Antibiotic Treatment Disrupts the Intestinal Microbiota and Intestinal Development in Neonatal Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Chung-Ming Chen ◽  
Hsiu-Chu Chou ◽  
Yu-Chen S. H. Yang
Keyword(s):  
Drinking Water ◽  
Antibiotic Treatment ◽  
Intestinal Microbiota ◽  
Relative Abundance ◽  
Cellular Proliferation ◽  
Nuclear Antigen ◽  
Control Group ◽  
Antibiotic Administration ◽  
Intestinal Development ◽  
Neonatal Mice

Maternal antibiotic treatment (MAT) during prenatal and intrapartum periods alters the bacterial composition and diversity of the intestinal microbiota of the offspring. The effect of MAT during pregnancy on the intestinal microbiota and its relationship with intestinal development remain unknown. This study investigated the effects of MAT during pregnancy on intestinal microbiota, injury and inflammation, vascularization, cellular proliferation, and the intestinal barrier in neonatal mice. At timed intervals, we fed pregnant C57BL/6N mice sterile drinking water containing antibiotics (ampicillin, gentamicin, and vancomycin; all 1 mg/ml) from gestational day 15 to delivery. The control dams were fed sterile drinking water. Antibiotic administration was halted immediately after birth. On postnatal day 7, the intestinal microbiota was sampled from the lower gastrointestinal tract and the ileum was harvested for histology, Western blot, and cytokines analyses. MAT significantly reduced the relative abundance of Bacteroidetes and Firmicutes and significantly increased the relative abundance of Proteobacteria in the intestine compared with their abundances in the control group. MAT also significantly increased intestinal injury score and cytokine levels, reduced the number of intestinal goblet cells and proliferating cell nuclear antigen-positive cells, and reduced the expressions of vascular endothelial growth factor and tight junction proteins. Therefore, we proposed that maternal antibiotic exposure during pregnancy disrupts the intestinal microbiota and intestinal development in neonatal mice.

scholarly journals Effects of active dry yeast on growth performance, rumen fermentation characteristics and slaughter performance in beef cattle

2019 ◽  
Author(s):  
Siqiang Liu ◽  
Mei Yuan ◽  
Kun Kang ◽  
Zhisheng Wang ◽  
Lizhi Wang ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Relative Abundance ◽  
Average Daily Gain ◽  
Neutral Detergent Fiber ◽  
Carcass Weight ◽  
Control Group ◽  
Microbial Composition ◽  
Significant Difference ◽  
Slaughter Performance ◽  
Active Dry Yeast

AbstractThis study aimed to investigate the effects of active dry yeast (ADY) on rumen microbial composition and slaughter performance of beef cattle. Thirty-two finishing beef cattle (simmental crossbred cattle ♂ × cattle-yaks ♀), with an average body weight of 110 ± 12.85 kg, were randomly assigned to one of four treatments: the low plane of nutrition group (Control), low plane of nutrition group + ADY 2 g/head/d (ADY2), low plane of nutrition group + ADY 4 g/head/d (ADY4) and high plane of nutrition group (HPN). ADY supplementation increased average daily gain (P<0.001), and the carcass weight of ADY4 group had no significant difference with HPN group (P>0.05). The serum glutamic-pyruvic transaminase activity in control and ADY4 group was higher than HPN group (P=0.001). The neutral detergent fiber (P=0.022) and acid detergent fiber (P=0.043) digestibility in HPN group was greater than control, but no difference was obtained among ADY2, ADY4 and HPN group (P>0.05). The rumen ammonium nitrogen content in control was greater than ADY2 and ADY4 group (P=0.003), and no difference was obtained ADY2, ADY4 and HPN group (P>0.05). The propionic acid content in the rumen in ADY2, ADY4, and HPN group were greater than control group (P<0.001). The simpson (P=0.014) and shannon (P=0.045) indexes in control and HPN group were greater than ADY4 group. At the phylum level, the relative abundance of Firmicutes in the HPN group was higher than ADY4 group (P=0.015). At the genus level, HPN and ADY4 were clustered together, and the relative abundance of Ruminococcaceae UCG-002 in ADY4 group was higher than control and HPN group (P=0.004). In conclusion, supplementation ADY 4 g/head/d shift the rumen microbial composition of beef cattle fed low plane of nutrition to a more similar level with cattle fed with HPN diet, produced comparable carcass weight with HPN diet.

scholarly journals Drinking water supplemented with wood vinegar on growth performance, intestinal morphology, and gut microbial of broiler chickens

2021 ◽  
Vol 14 (1) ◽  
pp. 92-96
Author(s):  
Kornkamon Hanchai ◽  
Tassanee Trairatapiwan ◽  
Rachakris Lertpatarakomol
Keyword(s):  
Drinking Water ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Intestinal Morphology ◽  
Control Group ◽  
Broiler Chicks ◽  
Plant Materials ◽  
Wood Vinegar ◽  
Significant Difference

Background and Aim: Wood vinegar is a product generated from the combustion and distillation of wood and other plant materials. It has been shown to suppress bacteria, resulting in healthier livestock and increased yields. This study aimed to determine the efficacy of drinking water supplemented with wood vinegar on growth performance, intestinal health, and gut microbial of broilers. Materials and Methods: A total of 120 Ross 308 1-day-old male broiler chicks were randomly distributed in a completely randomized experimental design. The study consisted of three treatments containing four replicates, with 10 birds in each. Treatments were given 0.5% and 1.0% (V/V) wood vinegar supplemented in drinking water, while no supplementation was given to the control group. The animals were raised in an open-house system. All groups were provided with a commercial diet and drinking water ad libitum. Analysis of variance was conducted using the general linear model procedure to compare the levels of wood vinegar supplementation in drinking water on growth performance, intestinal morphology, and gut microbial. Results: No significant differences (p>0.05) were found for body weight gain, feed intake, feed conversion ratio, and water consumption between groups during the starter (1-21 days old), grower (22-35 days old), and whole (1-35 days old) growth periods. Moreover, no significant differences in villi height and crypt depth (p>0.05) at 21 and 35 days of age were found. In addition, no significant difference in terms of lactic acid bacteria and Escherichia coli was found between the different treatments. Conclusion: Drinking water supplemented with wood vinegar was not found to have an effect on the growth performance and gut microbial of broiler chickens in the present study. However, the supplementation of wood vinegar in drinking water could improve intestinal morphology.

scholarly journals Role of Tryptophan-Metabolizing Microbiota On Diarrhea Mice Caused by Folium sennae Extracts

2020 ◽  
Author(s):  
Chenyang Zhang ◽  
Haoqing Shao ◽  
Dandan Li ◽  
Nenqun Xiao ◽  
Zhoujin Tan
Keyword(s):  
Cytochrome P450 ◽  
Intestinal Microbiota ◽  
Bacterial Flora ◽  
Shannon Index ◽  
Tryptophan Metabolism ◽  
Control Group ◽  
Cytochrome P450 Enzymes ◽  
Significant Difference ◽  
Tryptophan Derivatives

Abstract Background: Although reports have provided evidence that diarrhea caused by Folium sennae can result in intestinal microbiota diversity disorder, the intestinal bacterial characteristic and specific mechanism are still unknown. The objective of our study was to investigate the mechanism of diarrhea caused by Folium sennae, which was associated with intestinal bacterial characteristic reshaping and metabolic abnormality. Results: For the intervention of Folium sennae extracts, Chao1 index and Shannon index were statistical decreased. The Beta diversity clusters of mice interfered by Folium sennae extracts were distinctly separated from control group. Combining PPI network analysis, cytochrome P450 enzymes metabolism was the main signaling pathway of diarrhea caused by Folium sennae. Moreover, 10 bacterial flora communities had statistical significant difference with Folium sennae intervention: the abundance of Paraprevotella, Streptococcus, Epulopiscium, Sutterella and Mycoplasma increased significantly; and the abundance of Adlercreutzia, Lactobacillus, Dehalobacterium, Dorea and Oscillospira reduced significantly. 7 of the 10 intestinal microbiota communities were related to the synthesis of tryptophan derivatives, which affected the transformation of aminotryptophan into L-tryptophan, leading to abnormal tryptophan metabolism in the host. Conclusions: Folium sennae targeted cytochrome P450 3A4 to alter intestinal bacterial characteristic and intervene the tryptophan metabolism of intestinal microbiota, such as Streptococcus, Sutterella and Dorea, which could be the intestinal microecological mechanism of diarrhea caused by Folium sennae extracts.

scholarly journals Effects of mushroom waster medium and stalk residues on the growth performance and oxidative status in broilers

2021 ◽  
Vol 34 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Y. C. Hsieh ◽  
W. C. Lin ◽  
W. Y. Chuang ◽  
M. H. Chen ◽  
S. C. Chang ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Feed Additives ◽  
Control Group ◽  
Heme Oxygenase 1 ◽  
Antioxidant Genes ◽  
Significant Difference ◽  
Functional Components

Objective: The study developed mushroom stalk residues as feed additives in the broiler diet for improving the growth performance and immunity of broilers as well as to increase the value of mushroom stalk residues.Methods: In total, 300 ROSS 308 broilers were randomly allocated into fifteen pens with five dietary treatments: i) control, basal diet; ii) CMWM, supplemented with 1% <i>Cordyceps militaris</i> waster medium (CM); iii) CMPE, supplemented with 0.5% CM+0.5% <i>Pleurotus eryngii</i> stalk residue (PE); iv) CMPS, supplemented with 0.5% CM+0.5% <i>Pleurotus sajorcaju</i> stalk residue (PS); v) CMFV, supplemented with 0.5% CM+0.5% <i>Fammulina velutipes</i> stalk residue (FV).Results: The chemical analysis results showed that CM extracts, PE extracts, PS extracts, and FV extracts contain functional components such as polysaccharides and phenols and have both 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate scavenging and Ferrous scavenging capacities. The group CMWM saw increased body weight gain and feed conversion rate and the promotion of jejunum villus growth, but there is no significant difference in the intestinal bacteria phase. Antioxidant genes in the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)- antioxidant responsive element pathway among the groups are significantly higher than that of the control group, especially in group CMWM.Conclusion: The mushroom stalk residues have antioxidant functional components, can improve the intestinal health and body weight gain of chickens, and can activate the antioxidant pathway of Nrf2 to increase the heme oxygenase-1 expression. The treatment with 1% CM was the most promising as a feed additive.

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