A comprehensive survey on the occurrence of mycotoxins in maize dried distillers' grain and solubles sourced worldwide

2012 ◽  
Vol 5 (1) ◽  
pp. 83-88 ◽  
Author(s):  
I. Rodrigues ◽  
L. Chin
Keyword(s):  
Limit Of Detection ◽  
Raw Material ◽  
Alternative Ingredients ◽  
Comprehensive Survey ◽  
Negative Impacts ◽  
Contamination Levels ◽  
Distillers Grain ◽  
Aflatoxin B ◽  
Dried Distillers Grain ◽  
By Products

As cereal and protein sources suffer a price increase worldwide, the use of alternative ingredients in feeds has become increasingly appealing to the animal industry. Dried distillers' grain and solubles (DDGS) have been one of the ingredients which demand has dramatically increased over the last few years. In fact, the supply of maize DDGS is expected to increase to about 38.6 mmt by 2019-2020. The presence of mycotoxins in these by-products has been a matter of concern raised by their ubiquitous use. A rule of thumb typically used in the field is that the concentration of mycotoxins in the final by-product is about three times higher than that of the original raw material, which may be scientifically backed up by the fact that the remaining portions within the by-product are those which had a higher concentration of mycotoxins (outer portions of the grain). This paper is the outcome of a five-year study during which a total of 409 maize DDGS samples sourced worldwide were analysed for the mycotoxins of major importance which cause the great negative impacts in animal performance and health: aflatoxins (a sum of aflatoxin B1, B2, G1, G2), zearalenone, deoxynivalenol, a sum fumonisins B1 and B2 and ochratoxin A. From the total of samples tested, only 2% of the analysed DDGS showed contamination levels below the limit of detection (negative samples). 6% of samples had the presence of 1 mycotoxin only and the majority (92% of the samples) was contaminated with 2 or more mycotoxins.

69 Occurrence of Mycotoxins in 2020 US Corn Grain and Corn By-product Feeds

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 36-37
Author(s):  
Paige N Gott ◽  
Erin Schwandt ◽  
Shelby M Ramirez ◽  
Ursula Hofstetter ◽  
Raj Murugesan
Keyword(s):  
Limit Of Detection ◽  
Animal Health ◽  
Contamination Level ◽  
Negative Effects ◽  
Mycotoxin Contamination ◽  
Contamination Levels ◽  
And Performance ◽  
Harvest Year ◽  
By Products ◽  
Corn Grain

Abstract Mycotoxins are fungal metabolites that limit animal health and performance through various negative effects including reduced feed intake, diarrhea, and compromised immune function. These toxic metabolites have been detected in a broad range of feedstuffs worldwide. This study investigated mycotoxin occurrence and contamination levels in U.S. corn grain samples marked as 2020 harvest year and corn by-product feed samples submitted since October 2020. Samples were screened via LC-MS/MS for six major mycotoxin groups including: aflatoxins, type A trichothecenes, type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin A. Results from samples that were above the limit of detection for each respective mycotoxin group were analyzed using the GLIMMIX procedure (SAS 9.4, Cary, NC) to test the effect of harvest year on mean contamination level. Results are presented in Table 1. Occurrence levels of B-Trich, ZEN, and FUM in 2020 corn grain are numerically lower than those from 2019. Mean B-Trich level (parts per billion, ppb) for corn grain samples harvested in 2020 was similar to 2019 crop year. Contamination levels of FUM and ZEN were similar for 2020 crop as compared to prior harvest years. Mycotoxin occurrence in corn by-product feeds continued to be high at 99, 85, and 95% for B-Trich, ZEN, and FUM, respectively. Mean B-Trich and ZEN levels in 2020 corn by-products were similar to respective contamination levels in 2018, but were lower than 2019 samples. Mean FUM contamination in corn by-products has been similar across reported years. Preliminary results of the 2020 corn grain survey indicate mean mycotoxin contamination levels are similar to those observed the past two crop years while levels of B-Trich and ZEN in corn by-product feeds are reduced from 2019. Corn-based feed ingredients may present challenges to producers due to the presence of mycotoxin contamination, therefore, continued monitoring is warranted.

Co-occurrence of mycotoxins, including aflatoxins, trichothecenes, and zearalenone, in Chinese feedstuffs collected in 2013 and 2014

2019 ◽  
Vol 12 (1) ◽  
pp. 77-88 ◽  
Author(s):  
X. Han ◽  
W. Xu ◽  
F. Li ◽  
J. Xu
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Tandem Mass ◽  
Distillers Grain ◽  
Aflatoxin B ◽  
Dried Distillers Grain ◽  
Ionization Tandem Mass Spectrometry

A total of 357 feedstuff samples, including cottonseed meal plus soybean meal (CSM), wheat bran (WB) and dried distillers grain with solubles (DDGS) collected from 14 provinces in China in 2013 and 2014 were analysed for the co-occurrence of mycotoxins, including 4 aflatoxins, 7 trichothecenes, and zearalenone (ZEA), by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS). Deoxynivalenol (DON) was the most prevalent mycotoxin found in Chinese feedstuffs, followed by ZEA and aflatoxin B1 (AFB1). It was found that out of 357 samples, 27 (7.6%), 11 (3.1%) and 5 (1.4%) samples were positive for DON, ZEA and AFB1 at levels exceeding the Chinese regulatory limit of 1000 μg/kg, 500 μg/kg, 50 μg/kg for DON, ZEA, and AFB1, respectively. DON, ZEA, and AFB1 levels ranged from 1,005.5 to 2,893.1 μg/kg, 510.8 to 4,845.9 μg/kg, and 53.9 to 72.9 μg/kg, respectively. These samples were more easily co-contaminated by other mycotoxins, such as DON, ZEA, AFB1, AFB2, 3-acetyldeoxynivalenol (3-ADON), and 15-ADON. CSM was more easily contaminated by aflatoxins than WB and DDGS, and WB was more easily contaminated by DON and ZEA. Besides, 339 samples (95.0%, 339/357) were contaminated by at least two mycotoxins, and 16 samples (4.5%, 16/357) were only contaminated by one mycotoxin, and 2 samples (0.6%, 2/357) were not contaminated by any mycotoxin studied. There were 30, 66, and 66 different mycotoxin combination types in CSM, WB, and DDGS, respectively, but DON+3-ADON+15- ADON+ZEA was the most frequent mycotoxin combination, with a frequency of 13.16% (5/38) in CSM, 17.29% (23/133) in WB, and 18.8% (35/186) in DDGS. These results indicate that mycotoxin co-occurrence is very common in Chinese feedstuffs, and it is very important to conduct multi-mycotoxin monitoring in Chinese feedstuffs.

Comparison of wheat-based dried distillers’ grain with solubles to barley as an energy source for backgrounding cattle

2008 ◽  
Vol 88 (4) ◽  
pp. 721-724 ◽  
Author(s):  
J. J. McKinnon ◽  
A. M. Walker
Keyword(s):  
Energy Source ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Barley Grain ◽  
Dry Matter Intake ◽  
Cereal Grains ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
Daily Gain ◽  
By Products

Replacement of barley grain with wheat-based dried distillers’ (DDGS) grain with solubles at 25 and 50% of the total ration dry matter increased average daily gain and gain efficiency of backgrounding steers. No differences in dry matter intake or composition of gain were observed. The results of this trial indicate that barley grain can be replaced by wheat-based DDGS at up to 50% of the diet dry matter in backgrounding rations without any adverse effects on cattle performance. The economics of feeding DDGS at these levels would depend on availability and price relative to barley and other cereal grains. Key words: Dried distillers' grain, beef cattle, feedlot, ethanol, by-products

scholarly journals Enrichment of Protein Content in Rice-Based Dried Distillers’ Grain from Food-Ethanol Factory by Chemical Method

2021 ◽  
Vol 31 (4) ◽  
pp. 1-6
Keyword(s):  
Protein Content ◽  
Dry Matter ◽  
Direct Extraction ◽  
Negative Effect ◽  
Animal Feeding ◽  
Precipitate Protein ◽  
Protein Components ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
By Products

Dried distillers’ grain (DDG) from rice-based alcohol factories contains relatively high protein (ca. 79% of dry matter). However, this vegetative protein source has only been used for animal feeding. To enhance the value of this by-product, i.e. toward application for the food industry, this study has applied different approaches for the enrichment of protein content in this by-products. These approaches were either using solvents to directly extract and precipitate protein or removing non-protein components in rice-based DDG. The results showed that the direct extraction and precipitation of protein was not effective as the removal of non-protein components. The use of NaOH 10 mM has increased protein content up to 87% of dry matter by washing out the non-protein components such as starch from DDG. Preliminary, the protein-enriched DDG was used up to 15% as an ingredient for cookies without negative effect on the taste or color of this product.

scholarly journals Physicochemical characterisation of kafirins extracted from sorghum grain and dried distillers grain with solubles related to their biomaterial functionality

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Umar Shah ◽  
Deepak Dwivedi ◽  
Mark Hackett ◽  
Hani Al-Salami ◽  
Ranjeet P. Utikar ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Physicochemical Properties ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Chemical Properties ◽  
X Ray ◽  
Distillers Grain ◽  
Sorghum Grain ◽  
Dried Distillers Grain ◽  
Polypeptide Profile

AbstractKafirin, the hydrophobic prolamin storage protein in sorghum grain is enriched when the grain is used for bioethanol production to give dried distillers grain with solubles (DGGS) as a by-product. There is great interest in DDGS kafirin as a new source for biomaterials. There is however a lack of fundamental understanding of how the physicochemical properties of DDGS kafirin having been exposed to the high temperature conditions during ethanol production, compare to kafirin made directly from the grain. An understanding of these properties is required to catalyse the utilisation of DDGS kafirin for biomaterial applications. The aim of this study was to extract kafirin directly from sorghum grain and from DDGS derived from the same grain and, then perform a comparative investigation of the physicochemical properties of these kafirins in terms of: polypeptide profile by sodium-dodecyl sulphate polyacrylamide gel electrophoresis; secondary structure by Fourier transform infra-red spectroscopy and x-ray diffraction, self-assembly behaviour by small-angle x-ray scattering, surface morphology by scanning electron microscopy and surface chemical properties by energy dispersive x-ray spectroscopy. DDGS kafirin was found to have very similar polypeptide profile as grain kafirin but contained altered secondary structure with increased levels of β-sheets. The structure morphology showed surface fractals and surface elemental composition suggesting enhanced reactivity with possibility to endow interfacial wettability. These properties of DDGS kafirin may provide it with unique functionality and thus open up opportunities for it to be used as a novel food grade biomaterial.

scholarly journals 71 Effects of timing of supplementation during late gestation in Bos indicus-influenced beef cows on post-weaning growth, immunity, and carcass characteristics of steer progeny

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 59-59
Author(s):  
Elizabeth Palmer ◽  
Marcelo Vedovatto ◽  
Juliana Ranches ◽  
Vinicius Gouvea ◽  
Rhaiza Oliveira ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Carcass Characteristics ◽  
Beef Cows ◽  
Late Gestation ◽  
Experimental Unit ◽  
Carcass Quality ◽  
Diarrhea Virus ◽  
Randomized Design ◽  
Distillers Grain ◽  
Dried Distillers Grain

Abstract This study evaluated the growth, immunity, and carcass characteristics of beef steers born to cows offered dried distillers grains (DDG) at different timing during late gestation. Approximately 84 d before calving (d 0), 84 mature Brangus cows were stratified by BW and BCS (422 ± 42 kg; 5.0 ± 0.14) and allocated into 1 of 6 bahiagrass pastures (14 cows/pasture). Treatments were randomly assigned to pastures: 1) no prepartum supplementation (CON); 2) 1 kg/d DDG from d 0 to 84 (LATE84); or 3) 2 kg/d from d 0 to 42 (LATE42). Calves were weaned at 261 ± 23 d of age. At weaning, 38 steers were vaccinated against bovine viral diarrhea virus (BVDV-1) and parainfluenza-3 (PI-3) and transported to a feedlot facility. Steers were penned according to cow prepartum pasture distribution and fed a similar diet until slaughter (d 587). Data were analyzed as complete randomized design using MIXED procedure of SAS, pasture as the experimental unit, treatment as fixed effect, and pasture(treatment) and steer(pasture) as random effects. Steer feedlot BW, ADG, DMI, and G:F did not differ (P ≥ 0.12) among treatments. Plasma haptoglobin concentrations were greater (P ≤ 0.03) for LATE42 and LATE84 vs. CON steers 2 d after feedlot entry. Plasma cortisol and seroconversion for BVDV-1 and PI-3 did not differ (P ≥ 0.15) among treatments. The percentage of carcasses grading Choice did not differ (P = 0.13), but carcasses grading low Choice were greater (P = 0.03) for CON vs. LATE42, with LATE84 being intermediate (91, 40, and 58%, respectively). Thus, prepartum dried distillers grain supplementation to B. indicus-influenced cows did not enhance feedlot growth but had subtle positive impacts on carcass quality and innate immunity in steer progeny. In addition, timing of dried distillers grain supplementation (last 84 d vs. first 42 d of late gestation) did not impact steer post-weaning feedlot performance and carcass quality.

22 Effects of Housing Cow-calf Pairs on Drylots vs Pasture on Calf Performance and Behavior Through Weaning

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 24-24
Author(s):  
Megan Myerscough ◽  
Wes Chapple ◽  
William T Meteer ◽  
Daniel W Shike
Keyword(s):  
Corn Silage ◽  
Time Interaction ◽  
Creep Feed ◽  
Calf Performance ◽  
Ad Libitum ◽  
Cow Calf ◽  
Distillers Grain ◽  
Dried Distillers Grain ◽  
And Behavior ◽  
Fence Line

Abstract The objectives were to analyze the effects of housing cow-calf pairs in drylots or pasture on calf performance and behavior through weaning. Simmental × Angus (2 yr; 108/yr; 81 ± 15.3 d postpartum) spring-calving cows were stratified by age, BW, BCS, and calf sex and allotted to six groups/yr. Groups were randomly allotted to 1 of 2 treatments: drylot (DL) or pasture (PAST). Calves in the drylot had ad libitum access to a diet consisting of corn silage, dried distillers grain, corn stalks, dry rolled corn, and soybean hulls. Calves on PAST received creep feed three weeks prior to weaning. Calf BW was measured on d 0, 55, and 110. Calves were fence-line weaned on d 110. Behavior was observed for two days after weaning. Data were analyzed using the MIXED procedure of SAS. Drylot calves had greater (P < 0.01) BW at d 55 and 110. There was treatment by time interactions (P < 0.01) for lying and eating on d 111. More PAST calves were lying at h 2 and 3 and eating at h 11 and 12. More DL calves were lying at h 9, 10, 11, and 12. More DL calves were eating at h 2 and tended to at h 3. There was also a treatment by time interaction (P < 0.01) for vocalizations on d 111. More PAST calves vocalized at h 1, however, DL calves vocalized more at h 4 and tended to at h 5. There was treatment by time interactions (P < 0.01) for eating and walking on d 112. More DL calves were eating at h 2, 3, 4, 7, and 9. More PAST calves were walking at h 1 and 2. There was also a treatment by time interaction (P < 0.01) for vocalizations on d 112. More PAST calves vocalized at h 1 and 10. In conclusion, housing cow-calf pairs in drylots improved preweaning performance and altered postweaning behavior.

43 Cow Performance and Lameness Are Affected by Housing in Drylots vs Pasture During the Summer

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 147-148
Author(s):  
Lucas Hofer ◽  
Megan Myerscough ◽  
Wes Chapple ◽  
Travis T Meteer ◽  
Keela Trennepohl ◽  
...  
Keyword(s):  
Milk Production ◽  
Binary Data ◽  
Body Condition Score ◽  
Hair Coat ◽  
Milk Protein Content ◽  
Condition Score ◽  
Milk Urea ◽  
Cow Performance ◽  
Distillers Grain ◽  
Dried Distillers Grain

Abstract The objective was to compare the performance of cows housed in drylots or on pasture. Spring-calving, Simmental × Angus cow-calf pairs (n = 108; 77 ± 18 days postpartum) were stratified by age, body weight (BW), body condition score (BCS), calving date, and calf sex. Cows were allotted into six groups which were randomly assigned to drylot or pasture. Drylot cows were limit-fed a ration consisting of corn silage, dried distillers grain, corn stalks, cracked corn, and a corn-based supplement to meet protein and energy requirements. Cows on pasture were rotationally grazed with access to free-choice mineral. Cows were artificially inseminated on day 0. Cow BW, BCS, hair coat scores, locomotion scores, and lameness treatments were evaluated throughout the 110-day experiment. Milk production and composition were evaluated on day 56. Data were analyzed using the MIXED and GLIMMIX (binary data) procedures of SAS. Artificial insemination and overall pregnancy rates did not differ (P ≥ 0.79) between groups. Drylot cows were 22 and 51 kg heavier (P ≤ 0.02) than pasture cows on days 83 and 110, respectively. Drylot cow BCS was greater (P = 0.03) on day 110. Hair coat scores were more desirable (P = 0.03) in drylot cows than pasture cows on day 110. Drylot cows had greater (P = 0.04) milk production than pasture cows. Pasture cows had greater (P ≤ 0.03) milk protein content and milk urea nitrogen. Although locomotion scores did not differ (P ≥ 0.45) on days 0 and 34, they were less desirable (P = 0.02) for the drylot cows on day 110. A greater (P = 0.02) percentage of drylot cows (33%) were treated for lameness than pasture cows (7%). Housing cows in drylots increased BW, BCS, and milk production, but resulted in poorer locomotion scores and increased lameness treatments.

scholarly journals Polyphenols From Vitis vinifera Lambrusco By-Products (Leaves From Pruning): Extraction Parameters Evaluation Through Design of Experiment

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290 ◽  
Author(s):  
Massimo Tacchini ◽  
Ilaria Burlini ◽  
Immacolata Maresca ◽  
Alessandro Grandini ◽  
Tatiana Bernardi ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Antioxidant Capacity ◽  
Design Of Experiment ◽  
Extraction Yield ◽  
Wine Industry ◽  
Raw Material ◽  
Extraction Time ◽  
Total Phenolic ◽  
Vitis Vinifera L ◽  
By Products

Vitis vinifera L. leaves from pruning are by-products of the wine industry and represent an important source of secondary raw material, thanks to their polyphenols content. Optimization of the extraction processes is a key factor for their valorization, and Design of Experiment (DOE) could be a tool to obtain the most performing extract in terms of polyphenols quality/quantity and bioactivity. Vitis vinifera Lambrusco leaves were subjected to ultrasound-assisted extractions guided by a 23 factorial design. Three independent parameters (% solvent, time of extraction, and solvent:solid ratio) were considered to evaluate the extraction process by analyzing the extraction yield, the total phenolic content (Folin-Ciocalteu assay), and the antioxidant capacity (DPPH assay). Moreover, the content of the main molecules was identified and quantified by reversed-phase high-performance liquid chromatography coupled with diode array detection and mass spectrometry. The DOE highlighted the best extraction conditions that showed slight changes considering the different evaluating parameters. The highest extraction yield was obtained by extraction with 100% water, 60 minutes of extraction time, and 30:1 solvent:solid ratio, but it was neither the richest in polyphenols nor antioxidant capacity. The latter 2 characteristics were associated with the extraction performed using 50% ethanol, 35 minutes of extraction time, and a 20:1 solvent:solid ratio. That extract also exhibited the highest quantity of flavonols.

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