A SYSTEM FOR SEGREGATING CANADIAN WHEAT INTO SUBGRADES OF GUARANTEED PROTEIN CONTENT

1976 ◽  
Vol 56 (3) ◽  
pp. 433-450 ◽  
Author(s):  
WILLIAM DUNNE ◽  
J. ANSEL ANDERSON

The Canadian Grain Commission decided to introduce segregation of the top grade of Canadian wheat into three subgrades aiming at guaranteed protein levels for export of 14.5, 13.5%, etc., or 14.0, 13.0%, etc. Primary samples taken on loading carlots at country elevators were mailed to central laboratories for single Kjeldahl determinations and results were made available at terminal elevators when carlots were unloaded. Monitoring was undertaken with official samples taken during unloading. Computer studies were made with paired primary and unload data for 9,800 carlots unloaded at six Pacific terminals over 12 wk. Sequential segregation from the top of the distribution showed that two subgrades at required levels could be obtained by adjusting minimum boundaries for each subgrade, and a third more variable residual subgrade also seemed satisfactory; but to obtain maximum uniformity of means for subgrades required that boundaries be adjusted (1) as the mean and distribution of the main grade (1 C.W.) changed from week to week, and (2) from terminal to terminal, irrespective of whether cars were pooled or distributed by company of origin. In accordance with regression theory, unload subgrade means were lower than corresponding primary means when segregation was undertaken above the 1 C.W. mean. Floating station means for earlier shipments could be substituted for missing primary samples with some loss of precision; complete substitution permitted segregation of only two subgrades. For controlling shipments from country points, there are 48 Blocks with 5–10 Train Runs in each. Both the 1 C.W. mean and the distribution for terminal unloads could be changed by directing shipments from selected Train Runs or Blocks, but this might cause segregation problems. As the 1 C.W. mean changes, segregation boundaries must be adjusted and amounts of subgrades changed substantially. For each system, segregation at even or half percentage levels, the boundaries and amounts for subgrades repeat themselves, one percentage unit lower for each one percentage unit drop in the 1 C.W. mean. These studies indicated that the proposed system would prove practicable. The Commission introduced it forthwith, using guaranteed levels of 15.0, 14.0%, etc. at Thunder Bay, and of 14.5, 13.5%, etc. at Vancouver.

1975 ◽  
Vol 55 (1) ◽  
pp. 1-18 ◽  
Author(s):  
W. DUNNE ◽  
J. A. ANDERSON

Studies prerequisite to introduction of protein segregation of wheat were undertaken in collaboration with the Canadian Grain Commission. The principal data comprised a 5% stratified sampling of all carlots of wheat unloaded in the Western Division since 1 August, 1939, with records of origin, destination, unloading date, grade, protein content, etc.. Nine crop years were selected with protein levels of 14.4, 14.1, 13.7, 13.7, 13.6, 13.5, 13.4, 13.1, and 12.2%, and data for the 94,894 carlots were read onto magnetic tape for computer studies. Nos. 1 and 2 Northern were combined to simulate the new grade of No. 1 Canada Western. The Commission’s original proposal, that this grade be divided into three subgrades by protein content, namely, over 14.2%, 13.0 to 14.2%, and under 13.0%, was then examined by superimposing it on data for the 9 selected yr. The middle subgrade was reasonably satisfactory, containing 40–60% of the main grade in all but extreme years, and with a protein level varying between 13.75 and 13.35%; but the other two subgrades varied in amount from less than 10% to over 50%, and the protein level of the upper subgrades varied from 14.5 to 15.1%. In accordance with revised thinking, systems were then examined for guaranteeing subgrades at 15.0, 14.0 and 13.0%, or at 14.5, 13.5 and 12.5% (and at higher or lower levels when available). If boundaries are set low enough to meet guarantees in low protein years, levels 0.25–0.6% above guarantees occur in high protein years. Three subgrades, though not necessarily the same three, differing in protein level by 1.0% were feasible in all years, but strict uniformity in protein level could not be achieved within subgrades with systems involving constant boundaries between subgrades. Promotion of carlots from one subgrade to the next, on unload at terminal elevators, was then examined. In effect, this involves moveable boundaries, e.g. to guarantee 14.0% protein, the middle subgrade would have boundaries of 13.8–14.8% when the main grade has a mean of 13.0% protein, and of 13.9–14.3% with a mean of 14.5%. By comparison with constant boundary systems, from 8 to 25% of all carlots would be promoted by one subgrade, and strict uniformity of protein levels is achieved for all but the bottom subgrade. New data were collected to examine the practicability of systems involving moveable boundaries, and these studies will be reported in a second paper.


2015 ◽  
Vol 22 (4) ◽  
pp. 177-180 ◽  
Author(s):  
Huseyin Bayazit ◽  
Erdinc Cicek ◽  
Salih Selek ◽  
Nurten Aksoy ◽  
I. Fatih Karababa ◽  
...  

Background: It has been determined that cannabis has adverse effects on brain tissue, and that increased S100 calcium binding protein B (S100B) blood levels are markers of neuronal damage. Therefore, the aim of this study was to evaluate the S100B levels in cannabis use disorder. Method: Thirty-two patients with cannabis use disorder and 31 matched healthy controls were enrolled in this study. Appropriate blood samples were taken from the enrolled subjects, and the serum S100B protein levels were measured with an electrochemiluminescence immunoassay for the quantification of the protein. Findings: We found significantly increased S100B protein levels in patients with cannabis use disorder. The mean serum concentration of S100B was 0.081 ± 0.018 μg/l in patients with cannabis use disorder, and 0.069 ± 0.018 μg/l in the control group (p = 0.008). Interpretation: Our data suggest that elevated S100B protein levels might indicate neuronal damage in the brains of people with cannabis use disorder.


2005 ◽  
Vol 289 (2) ◽  
pp. C372-C378 ◽  
Author(s):  
Roberto Justo ◽  
Jordi Boada ◽  
Margalida Frontera ◽  
Jordi Oliver ◽  
Jordi Bermúdez ◽  
...  

In the present study, we have investigated gender differences in rat liver mitochondrial oxidative metabolism. Total mitochondrial population (M) as well as the heavy (M1), medium (M3), and light (M8) mitochondrial fractions obtained by means of differential centrifugation steps at 1,000, 3,000, and 8,000 g, respectively, were isolated. Electron microscopic analysis was performed and mitochondrial protein content and cardiolipin levels, mitochondrial O2 flux, ATP synthase activity, mitochondrial membrane potential, and mitochondrial transcription factor A (TFAM) protein levels were measured in each sample. Our results indicate that mitochondria from females have higher protein content and higher cardiolipin levels, greater respiratory and phosphorylative capacities, and more-energized mitochondria in respiratory state 3. Moreover, protein levels of TFAM were four times greater in females than in males. Gender differences in the aforementioned parameters were more patent in the isolated heavy M1 and M3 mitochondrial fractions. The present study demonstrates that gender-related differences in liver mitochondrial function are due mainly to a higher capacity and efficiency of substrate oxidation, likely related to greater mitochondrial machinery in females than in males, which is in accord with greater mitochondrial differentiation in females.


2000 ◽  
Vol 80 (4) ◽  
pp. 633-642 ◽  
Author(s):  
Palle V. Rasmussen ◽  
Christian F. Børsting

The effect of different and shifting dietary protein levels on hair growth and the resulting pelt quality in mink was studied. Two groups of pastel female mink were fed either 59% (high protein, HP) or 40% (low protein, LP) of metabolisable energy (ME) from protein during pregnancy and lactation. Shortly after weaning, kits from females fed the LP diet were put on a new LP diet (21% protein of ME). Kits from females fed HP were randomly distributed to four experimental groups fed a new HP diet (34% protein of ME) and three of these groups were shifted to diets with 21% protein at different times during June until September. Skin biopsies were taken at 4, 6, 23 and, 29 wk of age. Histological techniques and computer-assisted light microscopy were used to determine the ratio of activity (ROA) of underfur and guard hairs, respectively, defined as the number of growing hairs as a percentage of the total number of hairs. The hair fibre length and thickness were determined by morphometric methods and correlated with fur properties of dried pelts judged by sensory methods. It was documented that 40% of ME from protein during pregnancy and lactation was sufficient for mink kits to express their genetic capacity to produce hair follicles. In males, a reduced protein level from the age of 15 wk or 22 wk until pelting disturbed moulting, indicated by a low ROA of underfur hairs at 23 wk, and consequently reduced the growth and development of the winter coat. A constantly low protein level from conception until the age of 29 wk did not disturb moulting, but led to a reduction of primeness and especially of the underfur length and fibre thickness of the winter coat. A low protein level from the age of 9 wk only reduced the thickness of the underfur fibres. Hair growth, final fur volume, and general quality of the winter coat of males were influenced negatively and to the same degree in all groups fed the LP diet in part of the growth period. The number of underfur hairs per area (hair density) of the winter coat was not influenced by the dietary treatment meaning that the protein content of 21% of ME in the LP diet was high enough for the mink to express its genetic capacity to develop hair follicles. However, this low protein content led to a reduction of hair fibre length and hair fibre thickness of the underfur. Overall, this study demonstrated that hair growth and hair properties in pelts are very dependent on the dietary protein supply in the period from 22 wk of age until pelting, irrespective of the supply in the preceding periods. Key words: Fur properties, hair fibres, nutrition, pelage, protein requirement


2020 ◽  
Vol 3 (2) ◽  
pp. 604-610
Author(s):  
Fajar Nur Atmaja

Soy milk is a beverage product that is currently being favored by the people of Indonesia, to increase the protein content in soy milk needs to be substituted for other ingredients using Moringa Oleifera leaf extract. The purpose of this study was to analyze the organoleptic acceptability of soy milk by giving Moringa Oleifera leaf extract. This research is quantitative research with experimental research design using the True Experiment research method. This research was conducted in December 2018 until February 2019 at the Food Quality and Safety Testing Laboratory of the Agricultural Product Technology Department of the Faculty of Agriculture, University of Brawijaya Malang to test the protein content while the Organoleptic Test was carried out at STIKeS Satria Bhakti Nganjuk. The sample size is 20 panelists. The independent variable in this study was Moringa leaf extract while the protein content of soy milk. Data was collected using an organoleptic sensory test assessment table and a protein content test sheet using the Kjeldahl method. Data were analyzed using ANNOVA test with the results of H0 rejected and H1 accepted with a significant value <0.05 The results of ANOVA analysis get F count value of 105,405 and F table for testing 20 samples to get F table 4.35. So that it can be concluded that the addition of Moringa leaf extract 0% to 10% Significant and simultaneous organoleptic. Addition of Moringa Oleifera leaf extract to soy milk affected organoleptic acceptability and increased protein content of soy milk in accordance with the percentage of Moringa leaf extract levels added.


2021 ◽  
Vol 922 (1) ◽  
pp. 012061
Author(s):  
C D Iskandar ◽  
Zainuddin

Abstract This study aims to determine the amount of protein content in Aceh cattle beef in the quadriceps muscle (chuck) and to find out the best storage between cold and frozen to protein levels of beef. This study used 10 samples of Aceh cattle beef parts of the quadriceps (chuck) which were divided into 3 treatment groups, group I meat was treated in fresh condition, Group II meat was treated in cold at 8 °C and Group III meat will be treated in frozen at -19 °C. Protein content in meat was analysed by the Independent Samples Test. The results showed that the amount of Aceh beef protein in the quadriceps (chuck) in fresh; 15, 47%, cold 10, 20% and frozen; 9, 97. It was concluded that storing meat in cold and frozen ways is affect the protein content. Frozen meat storage is better to keep protein content than Cold meat storage.


2008 ◽  
Vol 19 (3) ◽  
pp. 252 ◽  
Author(s):  
O. NISSINEN ◽  
P. KALLIAINEN ◽  
L. JAUHIAINEN

The development of the yield and nutritive value of timothy (Phleum pratense L.) both in the primary growth and in the regrowth were studied at MTT Plant Production Research, a unit of MTT Agrifood Research Finland, in Rovaniemi (66°35´N) in 1999–2001. The dry matter yield and leaf:stem ratio were measured from the crop samples, and the contents of crude protein and organic matter digestibility of both whole plant samples and leaf and stem fractions were analysed. In primary growth, the most rapid increase of dry matter, 220–240 kg ha-1 per day, was measured around the beginning of the heading stage. There was a very strong positive correlation between the proportion of stems and the amount of dry matter in the primary yield. The daily growth rate of the regrowth was less than half of that of the primary growth. The fastest decrease, 1 percentage unit per day, in crude protein content was measured at the pasture stage (4–5-leaf stage). During the entire sampling period, the average daily decline in crude protein content in the primary growth of timothy was 0.65 percentage units. The main cause for the rapid decline in crude protein content was the high proportion of stem matter and its low protein content. In the regrowth, during the last four weeks before the harvest, the average daily decline in crude protein content was 0.28 percentage units.The average decline in organic matter digestibility from early pasture stage to late silage stage was 0.9 percentage units per day. The most remarkable change was noticed at the growth stage of timothy when about the half of stems were heading and it was then that the digestibility decreased by more than one percentage unit per day. The rapid decline in organic matter digestibility was due to the low digestibility of stem matter. The daily change in forage digestibility in the regrowth was very small, on average 0.11%.;


Author(s):  
Dian Haryati ◽  
Lulu Nadhira ◽  
Humairah Hera ◽  
Nurlaila Abdullah

Basically, gelatin is pure protein food ingredient, obtained from thermal collagen denaturation from animals. Gelatin is used as a stabilizer, gelling agent, binder, thickener, emulsifier, adhesive, whipping agent, and food wrap which is edible coating. Protein levels in fish skin determine the amount of collagen contained in skin tissue, so the baronang fish skin has a considerable opportunity as a source of collagen to be hydrolyzed into gelatin. This study aimed to determine the characteristics of gelatin produced from baronang fish skin by enzymatic extraction and to determine the best treatment of a combination of enzyme concentrations with extraction time. Making gelatine was carried out by hydrolysis using bromelin enzyme at a concentration of 1%, 1.5% and 2% with extraction time of 2 hours, 4 hours and 6 hours. The result, obtained gelatine with water content ranging from 3.13-5.83%, the water content ranged from 0.17-3.56%, ash content 0.35 - 3.65% and protein levels ranged from 91-94, 72%. The chemical characteristics of gelatine from baronang fish skin have a yield of up to 6%, water content <6%, ash content <4% and protein content reaching 94%. The best treatment was obtained at 1% enzyme concentration and extraction for 4 hours with a protein content of 94.72%.


Author(s):  
I. L. Okoroiwu ◽  
Jane Ugochi Chinedu-Madu ◽  
Emmanuel Ifeanyi Obeagu ◽  
C. C. N. Vincent ◽  
O. M. T. B. Ochiabuto ◽  
...  

The study was done to determine iron status, haemoglobin and protein levels of pregnant women in owerri metropolis. A total of 100 pregnant women were recruited for this study. The mean Hb levels in group 1, group 2, and group 3· were 12.00±1.68g/dl, 10.06±1.J4g/dl and 10.96±1.19g/dl respectively. The mean Serum ferritin level of group 1 was 67.00±88.38ng/ml, group 2, 52.48±52.47ng/ml and group 3, 51.26±48.70ng/ml. The mean Serum iron in group 1, 2 and 3 were 46.72±16.41 g/dl, 79.59±63.24 g/dl and 83.35±53.04 g/dl respectively. In group 1, 2 and 3 the mean results. ( g/dl) of TIBC were 295.58 ± 109.53, 324.06 ± 178.00 and 319.88 ± 92.95 and % T.S (%) were 18.78 ± 11.77,26.59 ± 19.40 and 17.97 ± 10.87 percent respectively. The mean total protein was group 1,6.83±l1.77g/dl, group 2,6.39±0.70g/dl and group 3, 6.39 ±0.98 g/dl while the mean albumin (g/dl) in group 1, 2 and 3 were 4.84±0.47, 4.13±0.28 and 4.14±0.29 respectively. The mean values of globulin (g/dl) were 1.98 ± 0.91, 2.29 ± 0.87 and 1.89 ± 0.90 in groups 1, 2 and 3 respectively. As gestational age increased; serum ferritin, total protein, and albumin levels decreased while serum" iron and TIBC increased. The differences in the mean results between the groups were statistically significant (p<0.05) while % T.S and globulin levels when compared showed no significant difference (p>0.05). Iron status showed no statistical difference with increasing parity (p>0.05). However, from this study iron deficiency anaemia was most prevalent in second trimester; hence iron status estimation should be an integral part of routine antenatal care test during second trimester of each pregnancy for proper assessment and management of iron deficiency anaemia in pregnancy.


1999 ◽  
Vol 77 (11) ◽  
pp. 1822-1828 ◽  
Author(s):  
Andrew G McAdam ◽  
John S Millar

Growth and female maturation appear to be limited by the availability of dietary protein in natural populations of deer mice (Peromyscus maniculatus borealis) in the Kananaskis Valley, Alberta. We examined the effects of dietary protein content on nestling growth rates and sexual maturation of female deer mice in two laboratory experiments. In the first, mice whose mothers were fed a low-protein mixture of sunflower seeds and oats (14% protein) exhibited slow growth prior to weaning and those fed high-protein cat food (30% protein) postweaning showed compensatory growth. Preweaning but not postweaning diet quality affected the proportion of females who were sexually mature at 42 days of age. Therefore, while deficient nestling growth can be compensated for, the effects of a low-quality maternal diet during lactation may have lasting effects on the maturation of female offspring. In the second experiment, mice raised on isocaloric diets of 14, 20, and 30% protein did not differ in growth as nestlings or juveniles. Differences among the three diets in the proportion of mature females at 42 days did not correspond to dietary protein levels as predicted. Dietary protein content from 14 to 30% appear to be sufficient for juvenile mice raised in captivity.


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