Prevalence and Genetic Diversity of Arcobacter in Food Products in the North of Spain

2013 ◽  
Vol 76 (8) ◽  
pp. 1447-1450 ◽  
Author(s):  
BARBARA NIEVA-ECHEVARRIA ◽  
IRATI MARTINEZ-MALAXETXEBARRIA ◽  
CECILIA GIRBAU ◽  
RODRIGO ALONSO ◽  
AURORA FERNÁNDEZ-ASTORGA
Keyword(s):  
Public Health ◽  
Genetic Diversity ◽  
Basque Country ◽  
Food Products ◽  
High Genetic Diversity ◽  
The North ◽  
Arcobacter Butzleri ◽  
North Of Spain ◽  
The Impact ◽  
The Relationship

The bacterial contamination of food products can cause serious public health problems. Interest in Arcobacter contamination has increased due to the relationship between these bacteria and human enteritis. We studied the prevalence and genetic diversity of Arcobacter species at the retail level in the province of Alava in Basque Country, Spain. The results showed a high genetic diversity and indicated the regular presence of the main Arcobacter spp. associated with human enteric illness in food products. Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii were detected with an overall prevalence close to 40% and were isolated from 15 (42.8%) fresh cow's milk samples, 12 (73.3%) shellfish samples, 11 (55%) chicken samples, 2 (10%) pork samples, and 1 (5%) beef sample. The results indicate the need to investigate the impact of Arcobacter spp. on public health.

Multilocus sequence typing reveals genetic diversity of foodborne Arcobacter butzleri isolates in the North of Spain

2014 ◽  
Vol 191 ◽  
pp. 125-128 ◽  
Author(s):  
Rodrigo Alonso ◽  
Cecilia Girbau ◽  
Irati Martinez-Malaxetxebarria ◽  
Aurora Fernández-Astorga
Keyword(s):  
Genetic Diversity ◽  
Multilocus Sequence Typing ◽  
The North ◽  
Arcobacter Butzleri ◽  
North Of Spain

scholarly journals Comparison of Diplodia Tip Blight Pathogens in Spanish and North American Pine Ecosystems

2021 ◽  
Vol 9 (12) ◽  
pp. 2565
Author(s):  
Ana Aragonés ◽  
Tania Manzanos ◽  
Glen Stanosz ◽  
Isabel A. Munck ◽  
Rosa Raposo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genotypic Diversity ◽  
Economic Losses ◽  
Common Disease ◽  
Sample Type ◽  
High Genetic Diversity ◽  
The North ◽  
Low Genetic Diversity ◽  
Shoot Blight ◽  
North Of Spain

Diplodia tip blight is the most ubiquitous and abundant disease in Spanish Pinus radiata plantations. The economic losses in forest stands can be very severe because of its abundance in cones and seeds together with the low genetic diversity of the host. Pinus resinosa is not genetically diverse in North America either, and Diplodia shoot blight is a common disease. Disease control may require management designs to be adapted for each region. The genetic diversity of the pathogen could be an indicator of its virulence and spreading capacity. Our objective was to understand the diversity of Diplodia spp. in Spanish plantations and to compare it with the structure of American populations to collaborate in future management guidelines. Genotypic diversity was investigated using microsatellite markers. Eight loci (SS9–SS16) were polymorphic for the 322 isolates genotyped. The results indicate that Diplodia sapinea is the most frequent Diplodia species present in plantations of the north of Spain and has high genetic diversity. The higher genetic diversity recorded in Spain in comparison to previous studies could be influenced by the intensity of the sampling and the evidence about the remarkable influence of the sample type.

scholarly journals A tale of two parts of Switzerland: regional differences in the impact of the COVID-19 pandemic on parents

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Michelle Seiler ◽  
Georg Staubli ◽  
Julia Hoeffe ◽  
Gianluca Gualco ◽  
Sergio Manzano ◽  
...  
Keyword(s):  
Public Health ◽  
Regional Differences ◽  
Online Survey ◽  
Pediatric Emergency ◽  
The South ◽  
Public Health Agencies ◽  
The North ◽  
Regional Strategies ◽  
Health Agencies ◽  
The Impact

Abstract Background We aimed to document the impact of the coronavirus disease 2019 (COVID-19) pandemic on regions within a European country. Methods Parents arriving at two pediatric emergency departments (EDs) in North of Switzerland and two in South of Switzerland completed an online survey during the first peak of the pandemic (April–June 2020). They were asked to rate their concern about their children or themselves having COVID-19. Results A total of 662 respondents completed the survey. Parents in the South were significantly more exposed to someone tested positive for COVID-19 than in the North (13.9 and 4.7%, respectively; P <  0.001). Parents in the South were much more concerned than in the North that they (mean 4.61 and 3.32, respectively; P <  0.001) or their child (mean 4.79 and 3.17, respectively; P <  0.001) had COVID-19. Parents reported their children wore facemasks significantly more often in the South than in the North (71.5 and 23.5%, respectively; P <  0.001). Conclusion The COVID-19 pandemic resulted in significant regional differences among families arriving at EDs in Switzerland. Public health agencies should consider regional strategies, rather than country-wide guidelines, in future pandemics and for vaccination against COVID-19 for children.

scholarly journals Did people really drink bleach to prevent COVID-19? A tale of problematic respondents and a guide for measuring rare events in survey data

2020 ◽  
Author(s):  
Leib Litman ◽  
Zohn Rosen ◽  
Cheskie Rosenzweig ◽  
Sarah L. Weinberger-Litman ◽  
Aaron J. Moss ◽  
...  
Keyword(s):  
Public Health ◽  
Best Practices ◽  
Health Outcomes ◽  
Survey Research ◽  
Iron Content ◽  
Heart Attack ◽  
Public Health Research ◽  
Similar Rate ◽  
The Impact ◽  
The Relationship

AbstractSociety is becoming increasingly dependent on survey research. However, surveys can be impacted by participants who are non-attentive, respond randomly to survey questions, and misrepresent who they are and their true attitudes. The impact that such respondents can have on public health research has rarely been systematically examined. In this study we examine whether Americans began to engage in dangerous cleaning practices to avoid Covid-19 infection. Prior findings reported by the CDC have suggested that people began to engage in highly dangerous cleaning practices during the Covid-19 pandemic, including ingesting household cleansers such as bleach. In a series of studies totaling close to 1400 respondents, we show that 80-90% of reports of household cleanser ingestion are made by problematic respondents. These respondents report impossible claims such as ‘recently having had a fatal heart attack’ and ‘eating concrete for its iron content’ at a similar rate to ingesting household cleaners. Additionally, respondents’ frequent misreading or misinterpreting the intent of questions accounted for the rest of such claims. Once inattentive, mischievous, and careless respondents are taken out of the analytic sample we find no evidence that people ingest cleansers to prevent Covid-19 infection. The relationship between dangerous cleaning practices and health outcomes also becomes non-significant once problematic respondents are taken out of the analytic sample. These results show that reported ingestion of household cleaners and other similar dangerous practices are an artifact of problematic respondent bias. The implications of these findings for public health and medical survey research, as well as best practices for avoiding problematic respondents in surveys are discussed.

scholarly journals Genetic diversity and drug susceptibility patterns of the Mycobacterium tuberculosis complex in Yunnan, China

2019 ◽  
Vol 39 (5) ◽  
Author(s):  
Ruolan Bai ◽  
Shuijing Chi ◽  
Xiaofei Li ◽  
Xiting Dai ◽  
Zhenhua Ji ◽  
...  
Keyword(s):  
Public Health ◽  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Public Health Problem ◽  
Drug Susceptibility ◽  
Drug Resistant ◽  
Global Public Health ◽  
High Genetic Diversity ◽  
Dominant Feature ◽  
Mdr Tb

AbstractTuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) which has been threatening global public health for many years. High genetic diversity is dominant feature of Mtb. Increasing cases of multidrug-resistant (MDR) tuberculosis (MDR-TB) is a serious public health problem to TB control in China. Spontaneous mutations in the Mtb genome can alter proteins which are the target of drugs, making the bacteria drug resistant. The purpose of the present study was to analyze the genotype of Mtb isolates from some areas in Yunnan, China and explore the association between genotypes and MDR-TB. Using spoligotyping, we identified Beijing genotypes, six non-Beijing genotypes and a number of orphan genotypes from 270 Mtb isolates from patients in Yunnan Province during 2014–2016. Of 270 Mtb isolates, 102 clinical Mtb strains were identified as drug-resistant (DR) by drug susceptibility testing (DST), among them, 52 MDR strains. Beijing genotypes occupied the highest MDR proportion (78.85%) followed by the orphan genotypes (15.38%). The characteristics of MDR strains showed high genetic diversity. The results will help to efficiently improve diagnosis and treatment and provide valuable information for Mtb molecular epidemiology.

Nationalist historiography and the English and Gaelic worlds in the late middle ages

1986 ◽  
Vol 25 (97) ◽  
pp. 1-18 ◽  
Author(s):  
Steven G. Ellis
Keyword(s):  
Middle Ages ◽  
Social Differences ◽  
Late Medieval ◽  
Modern Times ◽  
The North ◽  
Late Middle Ages ◽  
Historical Coverage ◽  
Different Cultures ◽  
The Impact ◽  
The Relationship

Much more so than in modern times, sharp cultural and social differences distinguished the various peoples inhabiting the British Isles in the later middle ages. Not surprisingly these differences and the interaction between medieval forms of culture and society have attracted considerable attention by historians. By comparison with other fields of research, we know much about the impact of the Westminster government on the various regions of the English polity, about the interaction between highland and lowland Scotland and about the similarities and differences between English and Gaelic Ireland. Yet the historical coverage of these questions has been uneven, and what at first glance might appear obvious and promising lines of inquiry have been largely neglected — for example the relationship between Gaelic Ireland and Gaelic Scotland, or between Wales, the north of England and the lordship of Ireland as borderlands of the English polity. No doubt the nature and extent of the surviving evidence is an important factor in explaining this unevenness, but in fact studies of interaction between different cultures seem to reflect not so much their intrinsic importance for our understanding of different late medieval societies as their perceived significance for the future development of movements culminating in the present.

Phenotypic and genotypic signature of saponin chemical composition in Chinese wild soybean (Glycine soja): revealing genetic diversity, geographical variation and dispersal history of the species

2018 ◽  
Vol 69 (11) ◽  
pp. 1126
Author(s):  
Yuya Takahashi ◽  
Xiang-Hua Li ◽  
Chigen Tsukamoto ◽  
Ke-Jing Wang
Keyword(s):  
Genetic Diversity ◽  
Chemical Composition ◽  
Genetic Structure ◽  
Southern China ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Ice Age ◽  
High Genetic Diversity ◽  
The North ◽  
Or Genes

Saponin chemical composition was phenotyped and genotyped, and saponin composition-based geographical genetic diversity and differentiation were evaluated in Chinese wild soybean (Glycine soja Sieb. & Zucc.). Thirty-two phenotypes and 34 genotypes were confirmed from 3805 wild soybean accessions. Eleven phenotypes (AaαK, AaαIK, AaαIJK, AaBcEαJ, AaBcαK, AbEαIJ, AbαK, AbαIK, AbαIJK, AbβHAb and Aβ0) were newly detected. Four genes had frequencies: Sg-1a 78.8% and Sg-1b 21.0% at the Sg-1 locus; Sg-4 30.7% and Sg-6e 13.7% at their respective loci. The north-eastern and southern populations showed high genetic diversity; the Northeast region contained more novel variants (AuAe, A0, A0Bc, αH, αI αJ, αK, and AbβHAb), and the southern populations contained high frequencies of the Sg-4 gene. Gene differentiation (Fst) analysis suggested that Sg-4 and four group-α saponin alleles or genes (Sg-6e, Sg-6h, Sg-6i, Sg-6j) were important factors influencing the genetic structure and differentiation in Chinese wild soybeans. Geographical differentiation was characterised mainly by latitudinal differences, with two primary groups (north and south) based on saponin genes. Chinese wild soybean accessions differed from Japanese and South Korean ones in genetic structure based on saponin composition, the latter two being likely to have spread from southern China in the glacial stages during the last Ice Age.

scholarly journals The Impact of Human Genetic Polymorphisms on Rotavirus Susceptibility, Epidemiology, and Vaccine Take

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 324 ◽  
Author(s):  
Sumit Sharma ◽  
Marie Hagbom ◽  
Lennart Svensson ◽  
Johan Nordgren
Keyword(s):  
Public Health ◽  
Genetic Diversity ◽  
Immune Response ◽  
Viral Infections ◽  
Phenotypic Diversity ◽  
Health Impact ◽  
Human Populations ◽  
Blood Group Antigens ◽  
Receptor Ligand ◽  
The Impact

Innate resistance to viral infections can be attributed to mutations in genes involved in the immune response, or to the receptor/ligand. A remarkable example of the latter is the recently described Mendelian trait resistance to clinically important and globally predominating genotypes of rotavirus, the most common agent of severe dehydrating gastroenteritis in children worldwide. This resistance appears to be rotavirus genotype-dependent and is mainly mediated by histo-blood group antigens (HBGAs), which function as a receptor or attachment factors on gut epithelial surfaces. HBGA synthesis is mediated by fucosyltransferases and glycosyltransferases under the genetic control of the FUT2 (secretor), FUT3 (Lewis), and ABO (H) genes on chromosome 19. Significant genotypic and phenotypic diversity of HBGA expression exists between different human populations. This genetic diversity has an effect on genotype-specific susceptibility, molecular epidemiology, and vaccine take. Here, we will discuss studies on genetic susceptibility to rotavirus infection and place them in the context of population susceptibility, rotavirus epidemiology, vaccine take, and public health impact.

scholarly journals Detection of Virulence Genes in Multidrug Resistant Enterococci Isolated from Feedlots Dairy and Beef Cattle: Implications for Human Health and Food Safety

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Frank Eric Tatsing Foka ◽  
Collins Njie Ateba
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Efflux Pump ◽  
Health Sector ◽  
Multidrug Resistant ◽  
North West ◽  
The North ◽  
Vancomycin Resistant ◽  
The Impact

The misuse/abuse of antibiotics in intensive animal rearing and communities led to the emergence of resistant isolates such as vancomycin-resistant enterococci (VREs) worldwide. This has become a major source of concern for the public health sector. The aim of this study was to report the antibiotic resistance profiles and to highlight the presence of virulence genes in VREs isolated from feedlots cattle of the North-West Province of South Africa. 384 faecal samples, 24 drinking troughs water, and 24 soil samples were collected aseptically from 6 registered feedlots. Biochemical and molecular methods were used to identify and categorise the enterococci isolates. Their antibiotic resistance profiles were assessed and genotypic methods were used to determine their antibiotic resistance and their virulence profiles. 527 presumptive isolates were recovered, out of which 289 isolates were confirmed asEnterococcussp. Specifically,E. faecalis(9%),E. faecium(10%),E. durans(69%),E. gallinarum(6%),E. casseliflavus(2%),E. mundtii(2%), andE. avium(2%) were screened after molecular assays.VanA(62%),vanB(17%), andvanC(21%) resistance genes were detected in 176Enterococcussp., respectively. Moreover,tetK(26),tetL(57),msrA/B(111), andmefA(9) efflux pump genes were detected in 138 VRE isolates.Multiple antibiotic resistances were confirmed in all the VRE isolates of this study; the most common antibiotic resistance phenotype wasTETR-AMPR-AMXR-VANR-PENR-LINR-ERYR.CylA,hyl,esp,gelE, andasa1virulence genes were detected in 86 VREs with the exception of vancomycin-resistantE. mundtiiisolates that did not display any virulence factor. Most VRE isolates had more than one virulence genes but the most encountered virulence profile wasgelE-hyl. Potentially pathogenic multidrug resistant VREs were detected in this study; this highlights the impact of extensive usage of antimicrobials in intensive animal rearing and its implications on public health cannot be undermined.

scholarly journals Quantifying the effect of human practices on S. cerevisiae vineyard metapopulation diversity

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marine Börlin ◽  
Olivier Claisse ◽  
Warren Albertin ◽  
Franck Salin ◽  
Jean-Luc Legras ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Diversity ◽  
Farming System ◽  
Conventional Farming ◽  
Global Effect ◽  
High Genetic Diversity ◽  
Tight Connection ◽  
Human Practices ◽  
The Impact ◽  
Very High

Abstract Saccharomyces cerevisiae is the main actor of wine fermentation but at present, still little is known about the factors impacting its distribution in the vineyards. In this study, 23 vineyards and 7 cellars were sampled over 2 consecutive years in the Bordeaux and Bergerac regions. The impact of geography and farming system and the relation between grape and vat populations were evaluated using a collection of 1374 S. cerevisiae merlot grape isolates and 289 vat isolates analyzed at 17 microsatellites loci. A very high genetic diversity of S. cerevisiae strains was obtained from grape samples, higher in conventional farming system than in organic one. The geographic appellation and the wine estate significantly impact the S. cerevisiae population structure, whereas the type of farming system has a weak global effect. When comparing cellar and vineyard populations, we evidenced the tight connection between the two compartments, based on the high proportion of grape isolates (25%) related to the commercial starters used in the cellar and on the estimation of bidirectional geneflows between the vineyard and the cellar compartments.

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