Uji Daya Hambat Ekstrak Etanol Bintang Laut Bertanduk (Protoreaster nodosus) Terhadap Pertumbuhan Bakteri Staphylococcus aureus

INTISARI Bintang laut bertanduk merupakan salah satu biota laut yang digunakan sebagai obat tradisional. Salah satunya adalah pengobatan infeksi. Infeksi merupakan salah satu penyebab penyakit yang sering terjadi di daerah yang beriklim tropis khususnya Indonesia. Salah satu infeksi yang sering terjadi adalah infeksi pada kulit yang disebabkan oleh bakteri Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol bintang laut bertanduk dapat menghambat pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental yang terdiri atas lima perlakuan dan tiga kali pengulangan dengan menggunakan metode pengujian paper disk. Sampel uji yang diteliti adalah bintang laut bertanduk (Protoreaster nodosus) yang dilarutkan dalam DMSO 10% hingga didapatkan varian konsentrasi 5%, 10%, dan 15% kemudian dilakukan uji daya hambat terhadap bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak bintang laut bertanduk menghambat pertumbuhan bakteri Staphylococcus aureus masing-masing konsentrasi 5%, 10% dan 15% yakni 5,99mm, 6,69mm dan 7,64mm. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bintang laut bertanduk(Protoreaster nodosus) dapat menghambat pertumbuhan bakteri Staphylococcus aureus secara signifikan. Kata kunci : Zona Hambat, Bintang Laut Bertanduk, Staphylococcus aureus. ABSTRACT Horned starfish are one of the marine biota used as traditional medicine. One of them is the treatment of infections. Infection is one of the causes of diseases that often occur in the tropics, especially Indonesia. One infection that often occurs is an infection of the skin caused by Staphylococcus aureus. This study aims to determine whether the horned starfish ethanol extract can inhibit the growth of Staphylococcus aureus bacteria. This type of research used in this study is an experimental study consisting of five treatments and three repetitions using a diskette testing method. The test sample studied was a horned starfish (Protoreasternodosus) dissolved in DMSO 10% to 5%, 10%, and 15% of the variant of concentration was obtained then the inhibitory test was performed on Staphylococcus aureus bacteria. The results showed that the horned starfish extract inhibited the growth of Staphylococcus aureus, each concentrations of 5%, 10% and 15% respectively 5.99 mm, 6.69 mm and 7.64 mm. Based on these results it can be concluded that the ethanol extract of the horned starfish (Protoreasternodosus) can significantly inhibit the growth of Staphylococcus aureus bacteria. Keywords: Inhibited zone, Horn Star, Staphylococcus aureus.

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UJI DAYA HAMBAT EKSTRAK DAN FRAKSI SPONS Aaptos Aaptos TERHADAP PERTUMBUHAN Escherichia coli, Staphylococcus aureus, dan Candida albicans

PHARMACON ◽

10.35799/pha.8.2019.29251 ◽

2019 ◽

Vol 8 (1) ◽

pp. 168

Author(s):

Sitti N Tunggali ◽

Herny E. I. Simbala ◽

Henki Rotinsulu

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Microbial Growth ◽

Ethanol Extract ◽

Chloroform Fraction ◽

Marine Biota ◽

Disc Diffusion ◽

Average Value

ABSTRACT Sponge Aaptos aaptos is a marine biota that has great potential, which can be applied, in the pharmaceutical field because of the presence of large compounds in inhibiting microbial growth. This study aims to determine the inhibitory activity of extracts and fractions of sponge Aaptos aaptos on microbial growth of Escherichia coli, Staphylococcus aureus, and Candida albicans. The samples were extracted by maceration with 96 % ethanol and fractioned with n-hexane, choloroform and methanol. Testing is done using the Disc Diffusion Agar method. Crude ethanol extract and fraction of sponge Aaptos aaptos showed the greatest antimicrobial activity against Staphylococcus aureus and categorized as strong, with an average value of 20.32 mm for ethanol extract with strong categories, chloroform fraction 13,28 mm with medium category and methanol fractions 18,48 mm strong category. Keyword: Aaptos aaptos, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans. ABSTRAK Spons Aaptos aaptos merupakan biota laut yang memiliki potensi sebagai antimikroba yang dapat diterapkan di bidang farmasi dengan kandungan senyawa yang besar dalam menghambat pertumbuhan mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas daya hambat dari ekstrak dan fraksi spons Aaptos aaptos terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus, dan Candida albicans. Sampel diekstraksi secara maserasi dengan etanol dan difraksinasi dengan pelarut n–heksan, kloroform dan metanol. Pengujian dilakukan dengan menggunakan metode Disc Diffusion Agar. Ekstrak kasar etanol dan fraksi dari Spons Aaptos aaptos menunjukkan aktivitas antimikroba paling besar terhadap Staphylococcus aureus dan dikategorikan kuat, dengan nilai rata – rata 20,32 mm untuk ekstrak etanol dengan kategori kuat, fraksi kloroform 13,28 mm, kategori sedang dan fraksi metanol 18,48 mm kategori kuat.Kata Kunci : Aaptos aaptos, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

Pharma Xplore : Jurnal Ilmiah Farmasi ◽

10.36805/farmasi.v4i1.618 ◽

2019 ◽

Vol 4 (1) ◽

pp. 278-287

Author(s):

Lia Fikayuniar ◽

Neni Sri Gunarti ◽

Mellya Apriliani

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Curcuma Longa ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus, ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,

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DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

JURNAL ILMIAH SAINS ◽

10.35799/jis.15.2.2015.10231 ◽

2015 ◽

Vol 17 (1) ◽

pp. 149

Author(s):

Fahri Bian ◽

Febby E.F. Kandou ◽

Marhaenus J. Rumondor

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Inhibition Zone Diameter ◽

E Coli ◽

Zone Diameter ◽

Paper Disk

DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coliABSTRAK Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun Schismatoglottis sp. terhadap bakteri Staphylococcus aureus dan Escherichia coli. Daya hambat ekstrak etanol Schismatoglottis sp. terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak etanol Schismatoglottis sp. menghambat pertumbuhan S. aureus pada konsentrasi 60% dan 90% dengan diameter zona hambat 16,10 mm dan 31,33 mm serta terhadap E. coli pada konsentrasi 30%, 60%, dan 90% dengan diameter zona hambat berturut-turut 11,72 mm, 16,38 mm, dan 29,27 mm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol Schismatoglottis sp. terhadap S. aureus pada konsentrasi 60% dan 90% masing-masing memiliki kekautan antibakteri termasuk kategori kuat dan sangat kuat, sedangkan terhadap E. coli pada konsentrasi 30% dan 60%, memiliki kekuatan antibakteri kategoti kuat sedangkan pada konsentrasi 90% memiliki kekuatan antibakteri kategori sangat kuat. Kata kunci : Schismatoglottis sp, Antibakteri, Staphylococcus aureus, Escherichia coli THE INHIBITION OF ETHANOL EXTRACT OF Schismatoglottis sp. AGAINST BACTERIA Staphylococcus aureus AND Escherichia coli. ABSTRACT This study aimed to determine the inhibition of ethanol extract of leaves Schismatoglottis sp. against S. aureus and E. coli. Inhibition of the ethanol extract of Schismatoglottis sp. against to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. Schismatoglottis sp. ethanol extract inhibit the growth of S. aureus at a concentration of 60% and 90% inhibition zone with a diameter of 16.10 mm and 31.33 mm and against E. coli at a concentration of 30%, 60%, and 90% with inhibition zone diameter respectively 11, 72 mm, 16.38 mm and 29.27 mm. Based on the results, it can be concluded that the ethanol extract Schismatoglottis sp. against S. aureus at concentrations of 60% and 90% respectively have antibacterial kekautan categorized as strong and very strong, while against E. coli at a concentration of 30% and 60%, have a strong kategoti antibacterial strength while at a concentration of 90% has antibacterial strength very strong category. Keywords: Schismatoglottis sp, antibacterials, Staphylococcus aureus, Escherichia coli.

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Poguntano Herba Extract Immunostimulant Activities (Picriafel-terraeLour). in Immunosupression Rats Infected by Staphylococcus aureus Against Total Leukocytes and Differential Leukocytes

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v4i1.5378 ◽

2021 ◽

Vol 4 (1) ◽

pp. 39-46

Author(s):

Anggitha Ningtias ◽

Rosidah ◽

Yuandani

Keyword(s):

Staphylococcus Aureus ◽

Medicinal Plant ◽

Ethyl Acetate ◽

Traditional Medicine ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Hexane Extract ◽

Flavonoid Compounds ◽

North Sumatra

Poguntano (Picriafel-terraeLour.) is a medicinal plant in North Sumatra that has been used in traditional medicine to treat degenerative diseases.Poguntano extract contains flavonoid compounds that have the potential to be developed into immunomodulators. This study showed the immunostimulant activity of poguntano herb n-hexane extract (PHNHE), poguntano herb ethyl acetate extract (PHEAE) and poguntano herb ethanol extract (PHEE) in immunosuppressed rats infected with Staphylococcus aureus. The evidence of poguntano herb as an immunomodulator was carried out by testing total leukocytes and differential leukocytes. Poguntano herb n-hexane extract (PHNHE) can not increase the total and differential leukocytes; Poguntano herb ethyl acetate extract (PHEAE) and poguntano herb ethanol extract (PHEE) can increase the total and differential leukocytes in immunosuppressed rats induced by Staphylococcus aureus.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MAHKOTA DEWA (Phaleria macrocarpa) TERHADAP STAPHYLOCOCCUS AUREUS

Jurnal Kesehatan : Jurnal Ilmiah Multi Sciences ◽

10.52395/jkjims.v9i02.162 ◽

2019 ◽

Vol 9 (02) ◽

pp. 134-139

Author(s):

Yunilda Rosa ◽

Masnir Alwi ◽

Monica Julianti

Keyword(s):

Staphylococcus Aureus ◽

Experimental Study ◽

Antibacterial Activity ◽

Inhibitory Concentration ◽

Skin Diseases ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Dilution Method ◽

Phaleria Macrocarpa

Phaleria macrocarpa is one of the medicinal plants that many people use as a traditional medicinal which has many benefits, one of which is as a medicine for skin diseases. The content of flavonoid compounds in Phaleria macrocarpa believed to have potential as an antibacterial. The purpose of this study was to know the antibacterial activity of the ethanol extract of the Phaleria macrocarpa to inhibized Staphylococcus aureus bacteria. This research is an experimental study carried out by the liquid dilution method (KHM)and disk diffusion (KBM) of the extract of the Phaleria macrocarpa is divided into 6 concentrations namely 5%, 10% and 15% (KHM) and 40%, 50% and 60% (KBM). The positive control used is clindamycin and the negative control is aquadest. Data analysis uses univariate. Based on observations of the minimum inhibitory concentration of the extract of the fruit of the Phaleria macrocarpa which is at of 15%, while the minimum killed concentration is formed at of 40% with an average of 6.36 mm, and an average of zones formed on the positive control of 9.53 mm. From the results of the study, it was concluded that the extract of the fruit of the Phaleria macrocarpa can inhibit and kill the growth of Staphylococcus aureus bacteria.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS Mycale Vansoesti DARI PERAIRAN PULAU MANTEHAGE MINAHASA UTARA TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus

PHARMACON ◽

10.35799/pha.10.2021.34040 ◽

2021 ◽

Vol 10 (2) ◽

pp. 889

Author(s):

Brigita Michelle Luntungan ◽

Defny S. Wewengkang ◽

Erladys Rumondor

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Chloroform Fraction ◽

Marine Biota ◽

Methanol Fraction ◽

A Value ◽

Activity Test ◽

And Staphylococcus Aureus

ABSTRACTSponges are multi-cell marine biota whose tissue and organ functions are very simple. In the development of medicine, sponges have been shown to contain active compounds as guiding compounds in the synthesis of the latest drugs. The purpose of this study was to determine the antibacterial activity of the extracts and fractions of the sponge Mycale vansoesti collected from the waters of Mantehage Island against Escherichia coli and Staphylococcus aureus bacteria. The sponge samples were extracted using the maceration method with 95% ethanol. The antibacterial activity test used the disc diffusion agar method of Kirby and Bauer. The results showed that Mycale vansoesti sponge produced antibacterial activity in all extracts and fractions used namely ethanol extract, chloroform fraction, n-hexane fraction, and methanol fraction against Escherichia coli and Staphylococcus aureus bacteria with a value of about 6.63-8.82 mm and included in the moderate category of inhibition, this is proven by the formation of a clear zone around the disc. Keywords: Mycale vansoesti, Antibacterial, Staphylococcus aureus, Escherichia coli ABSTRAKSpons merupakan biota laut multi sel yang fungsi jaringan dan organnya sangat sederhana. Dalam perkembangan pengobatan, spons terbukti mengandung senyawa aktif sebagai senyawa pemandu dalam sintesis obat-obatan terbaru. Tujuan dari penelitian ini untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi spons Mycale vansoesti yang diperoleh dari perairan Pulau Mantehage terhadap bakteri Escherichia coli dan Staphylococcus aureus. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 95%. Pengujian Aktivitas Antibakteri menggunakan metode difusi agar Kirby and Bauer. Hasil penelitian menunjukkan bahwa spons Mycale vansoesti menghasilkan aktivitas antibakteri pada semua ekstrak dan fraksi yang digunakan yaitu ekstrak etanol, fraksi kloroform, fraksi n-heksan, dan fraksi metanol terhadap bakteri Escherichia coli dan Staphylococcus aureus dengan nilai sekitar 6,63-8,82 mm dan termasuk dalam daya hambat kategori sedang. Hal ini dibuktikan dengan terbentuknya zona bening disekitar cakram. Kata Kunci : Mycale vansoesti, Antibakteri, Staphylococcus aureus, Escherichia coli

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AKTIVITAS ANTIBAKTERI EKSTRAK MIKROALGA Thalassiosira sp TERHADAP BAKTERI Staphylococcus aureus, Staphylococcus epidermidis dan Propionibacterium Acne

Jurnal Kimia Riset ◽

10.20473/jkr.v4i1.13314 ◽

2019 ◽

Vol 4 (1) ◽

pp. 62

Author(s):

Vina Juliana Anggraeni ◽

Titis Setyaning Wahyu ◽

Herni Kusriani ◽

Dewi Kurnia

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Staphylococcus Epidermidis ◽

Ethanol Extract ◽

Diffusion Method ◽

Marine Biota ◽

Disc Diffusion ◽

Propionibacterium Acne ◽

X 24

AbstrakPengembangan obat dan kosmetik dari biota laut kini tengah terjadi di dunia farmasi. Mikroalga Thalassiosira sp merupakan jenis mikroalga yang memiliki kandungan senyawa-senyawa bioaktif. Beberapa penelitian sebelumnya menunjukkan bawa mikroalga memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak mikroalga Thalassiosira sp terhadap 3 bakteri yaitu staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne di fasa n-heksan, etil asetat dan etanol. Ketiga bakteri ini dapat menyebabkan infeksi kulit. Mikroalga Thalassiosira sp dikultivasi menggunakan medium walne dan di panen pada hari ke-6 setelah penanaman. Pemanen mikroalga dilakukan dengan teknik sentrifuga. Ektrak dilakukan dengan cara maserasi bertingkat selama 3 x 24 jam. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram atau metode disc diffusion menurut Kirby-Bauer. Hasil ekstrak mikroalga Thalassiosira sp diperoleh paling banyak pada ekstrak etanol sebanyak 24,24%(b/b), ektrak etil asetat sebanyak 19,75%(b/b) dan paling sedikit adalah ekstrak heksan sebanyak 8,64% (b/b). Hasil uji difusi menunjukkan ekstrak n-heksan, etil asetat dan etanol mikroalga Thalassiosira sp memiliki aktivitas terhadap bakteri staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne yang ditunjukan dengan adanya zona bening. Kata kunci: mikroalga, Thalassiosira sp, antibakteri, infeksi kulit, metode difusi Abstract Development drugs and cosmetics from marine biota is now being happened in pharmacy word. Microalgae Thalassiosira sp is a type of microalgae that has a bioactive compounds. Several previous studies have shown the existence of microalgae which have antibacterial activity. This study aimed to study the antibacterial activity of extracts of microalgae Thalassiosira sp against 3 bacteria which is staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne in the n-hexane, ethyl acetate and ethanol phases. These three bacteria can cause skin infections. Microalgae Thalassiosira sp was cultivated using walne medium and harvested on the 7th day after planting. Microalgae harvesters are carried out by centrifuge techniques. The extract is done by multilevel maceration for 3 x 24 hours. Antibacterial testing was carried out by the paper diffusion method or Kirby-Bauer's disc diffusion method. The results of the crude extract of microalgae Thalassiosira sp were obtained at most in ethanol extract as much as 24.24% (w/w), extract of ethyl acetate at 19.75% (w / w) and at least hexane extract at 8.64% (w/w). The diffusion test results for n-hexane, ethyl acetate, and ethanol microalgae Thalassiosira sp extract have activity on staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne which are supported by clear zones. Keywords: mikroalge, Thalassiosira sp, antibacteria, skin infection, diffusion method

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Uji Daya Hambat Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) terhadap Bakteri Staphylococcus aureus

Jurnal Surya Medika ◽

10.33084/jsm.v4i2.565 ◽

2019 ◽

Vol 4 (2) ◽

pp. 51-59 ◽

Cited By ~ 1

Author(s):

Susi Novaryatiin ◽

Ahmad Ramli ◽

Syahrida Dian Ardhany

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Traditional Medicine ◽

Skin Diseases ◽

Ethanol Extract ◽

Disc Diffusion ◽

Central Kalimantan ◽

Onion Extract ◽

Onion Bulbs

Dayak Onion is a typical plant of Central Kalimantan. This plant has been traditionally used by the Dayak community as traditional medicine. Empirically, Dayak Onion bulbs are known to have properties to treat boils or skin diseases. The purpose of this study was to determine the ability of inhibition of Dayak Onion ethanol extract in inhibiting Staphylococcus aureus bacteria and to determine the effectiveness of the inhibition of Dayak Onion ethanol extract and concentration that could inhibit the growth of Staphylococcus aureus bacteria. This research was carried out by testing the antibacterial activity of Dayak Onion extract at levels of 1%, 5%, 10%, and 15% using the Disc Diffusion (Kirby-Bauer) method by using disc paper. The results showed that Dayak Onion ethanol extract was able to inhibit the growth of Staphylococcus aureus and effectively inhibited the growth of Staphylococcus aureus at concentrations of 1%, 5%, 10%, and 15% with inhibitory zones of 14.3 � 2.5 mm ; 16.6 � 1.7 mm; 16.2 � 2.0 mm; and 18.0 � 1.7 mm.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v6i2.369 ◽

2020 ◽

Vol 6 (2) ◽

pp. 246

Author(s):

Yayuk Bulam Sarifati ◽

Sjarif Ismail ◽

Khemasili Kosala

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antimicrobial Agents ◽

Statistical Tests ◽

Ethanol Extract ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Control Measurement ◽

Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values <0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

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