scholarly journals Activity test of fruit and pomegranate seeds (Punica granatum l) as a hepatoprotector against white male Wistar rats

2021 ◽  
pp. 220-224
Author(s):  
Nur Rahayuningsih ◽  
Sri Zulfah Zakiah Darajat ◽  
Saeful Amin
Keyword(s):  
White Male ◽  
Ethanol Extract ◽  
Test Dose ◽  
Punica Granatum ◽  
White Rats ◽  
Hepatoprotector Activity ◽  
Wistar Strain ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Pomegranate Seeds

Introduction: White pomegranate (P. granatum) is a species of the Punicaceae which is thought as hepatoprotector based on its antioxidant secondary metabolite compoundst. Aims: The purpose of study was to determine the hepatoprotector activity of white pomegranate fruit and seeds ethanol extract on male white rats Wistar strain. Methods: This research is laboratory experimental which rats were grouped into 5 groups randomly. Normal, negative (given Carboxymethylcellulose sodium 1% orally), test dose 1,2,3 (given extract at a dose of 50 mg /200 g bodyweight of rat, 100 mg/200 g bodyweight of rat and 200 mg/200 g bodyweight of rat in Carboxymethylcellulose sodium 1% orally). Negative, test dose 1,2,3 induced with paracetamol 180 mg/200 g bodyweight of rat. Results: From the statistical analysis, it was found that a significant difference between the negative with test dose 1, 2, and 3. The best hepatoprotector activity was produced by the test dose 3 with the percentage reduction in AST levels 91.62% and ALT 90.20%.

Pengaruh Pemberian Ekstrak Anggur Laut terhadap Penurunan Kadar Kolesterol LDL Rattus norvegicus Jantan yang Mendapat Diet Tinggi Lemak

2020 ◽  
Vol 17 (2) ◽  
pp. 192
Author(s):  
RONALDO LAU ◽  
SULISTIANA PRABOWO ◽  
RIAMI RIAMI
Keyword(s):  
Cholesterol Level ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
White Male ◽  
Standard Diet ◽  
High Fat ◽  
Caulerpa Racemosa ◽  
Significant Difference ◽  
Male Wistar Rats

<p align="justify"><strong>ABSTRACT</strong><strong></strong></p><p align="justify"><strong>Background</strong>: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (<em>Caulerpa racemosa</em>) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract  on decreasing LDL cholesterol of white male Wistar rats (<em>Rattus norvegicus</em>) fed with high fat diet.</p><p align="justify"><strong>Method</strong>:  24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15<sup>th</sup>-28<sup>th</sup> days. Then the blood LDL cholesterol level measured on the 29<sup>th</sup> day.</p><p align="justify"><strong>Result :</strong> One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl).</p><p align="justify"><strong>Conclusion: </strong>high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (<em>Caulerpa racemosa</em>) significantly decrease blood LDL cholesterol level.</p><p align="justify"> </p><p align="justify"><strong>Keywords :</strong>Sea grapes extract, LDL cholesterol, high fat diet</p>

scholarly journals THE THE TERATOGENIC EFFECTS OF ETHANOLIC EXTRACT OF BINTANGUR LEAVES (CALOPHYLLUM SOULATTRI BURM. F) ON FEMALE WHITE RATS

2019 ◽  
pp. 160-163
Author(s):  
INARAH FAJRIATY ◽  
HAFRIZAL RIZA ◽  
FAJAR NUGRAHA ◽  
FRENGKI FRIANTO
Keyword(s):  
Body Weight ◽  
Comparison Group ◽  
Ethanol Extract ◽  
Teratogenic Effect ◽  
Control Group ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
White Rats ◽  
Significant Difference ◽  
Skeletal Formation

Objectives: Drugs can cause undesired effects on the fetus during pregnancy, especially embryonic/organogenesis which could lead to defects in the fetus because some types of drugs can penetrate the placenta and will undergo biotransformation into a highly reactive compound that has the potential to become a teratogenic compound. The aim of this research was to examine the teratogenic effect of bintangur leaves (Calophyllum soulattri Burm. F) ethanol extract to Sprague Dawley strain white rats. Methods: The white rats are divided into four treatment groups: Control group was given carboxymethyl cellulose Na 1%, comparison group was given trimethoprim 360 mg/kg BW, C. soulattri leaves ethanol extract (CLE) 100 mg/kg BW, and CLE 500 mg/kg BW. The treatment was administrated since organogenesis period. Cesarian section was performed to pregnant rat at the 20th day to separate the fetuses. Observation covered body weight of pregnant rats, fetal biometric, morphological malformation, and skeletal formation. Results: CLE 100 mg/kg BW and 500 mg/kg BW did not cause any change in the number of a living fetus, body weight, and length of fetuses like the comparison group. Both doses of CLE shown have a normal skeletal formation. Resorption was found in the comparison group and CLE 100 mg/kg BW with the percentage was 65.21% and 6.67%. It was found that there is no significant difference (p<0.05) between both doses of CLE compared to control group. Conclusion: From the results, it is concluded that CLE did not have the teratogenic effect.

scholarly journals PENGARUH PEMBERIAN EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa-sinensis L.) TERHADAP JUMLAH SPERMATOZOA, BERAT BADAN, DAN BERAT TESTIS TIKUS JANTAN WISTAR (Rattus norvegicus)

2019 ◽  
Vol 19 (1) ◽  
pp. 6
Author(s):  
Nabila S Petta ◽  
Edwin De Queljoe ◽  
Rooije R.H. Rumende
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
The Body ◽  
Male Rats ◽  
White Rats ◽  
Hibiscus Rosa Sinensis ◽  
Randomized Design ◽  
Male Wistar Rats ◽  
Ethanol Extracts

PENGARUH PEMBERIAN EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa-sinensis L.) TERHADAP JUMLAH SPERMATOZOA, BERAT BADAN, DAN       BERAT TESTIS TIKUS JANTAN WISTAR (Rattus norvegicus)ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol kembang sepatu terhadap jumlah spermatozoa tikus jantan wistar (Rattus norvegicus). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan menggunakan 24 ekor tikus putih jantan galur wistar (Rattus norvegicus) yang dibagi atas beberapa kelompok dimana kelompok 1 sebagai kelompok kontrol tanpa perlakuan, kelompok 2, 3 dan 4 sebagai kelompok perlakuan dengan dosis secara berturut-turut 3,6 mg/ml; 7,2 mg/ml; dan 14,4 mg/ml. Perlakuan diberikan secara oral sekali sehari sebanyak 1 ml selama 50 hari sesuai siklus spermatogenesis. Variabel yang diamati yakni jumlah sel spermatozoa, berat badan, dan berat testis. Hasil penelitian ini menunjukkan bahwa ekstrak etanol kembang sepatu dapat menurunkan jumlah sel spermatozoa, serta menyebabkan adanya perbedaan berat badan dan berat testis namun, berdasarkan hasil analisis varians, ekstrak etanol daun kembang sepatu tidak dapat menurunkan jumlah sel spermatozoa, berat badan dan berat testis tikus putih jantan galur wistar (Rattus norvegicus) secara signifikan.Kata Kunci: Sel spermatozoa, Kembang sepatu (Hibiscus rosa-sinensis L.),                    Tikus jantan Wistar (Rattus novergicus) THE INFLUENCE OF THE ETHANOL EXTRACTS  OF GRANTING HIBISCUS (Hibiscus Rosa-sinensis L.) AGAINST THE NUMBER OF SPERMATOZOA, WEIGHT AND THE WEIGHT OF THE TESTES MALE WISTAR RATS (Rattus norvegicus) ABSTRACTThis research’s objective is to know the influence of injecting ethanol extract from a hibiscus into a number of common male rats (Rattus norvegicus). This research uses the approach of complete randomized design (CRD) onto 24 common white rats (Rattus norvegicus) that is divided into groups, where group 1’s approach is control without treatment, groups 2, 3, and 4’s approach is with treatment, with consecutive doses being 3.6 mg/ml; 7.2 mg/ml; and 14.4 mg/ml.  The treatment is induced orally as large as 1cc per day for a total of 50 days following the spermatogenesis cycle. The variables that are being observed are the amount of spermatozoon cells, body weight, and testicle weight. The results of this research indicates that ethanol extract from hibiscuses, from a quantity perspective, can decrease spermatozoon cells, and it may also influence the body weight and testicle weight of the subject, in this case are common rats (Rattus norvegicus) but, from the mathematical results from Analysis Of Variance, ethanol extract from the leaves of a hibiscus cannot decrease the amount of spermatozoon, body weight, and testicle weight  of a common white rat (Rattus norvegicus).Keywords: Spermatozoon Cells, Hibiscus (Hibiscus rosa-sinensis L.), Common (white) rat (Rattus novergicus)

scholarly journals Difference of Effectiveness Acarbose with Ethanol Extract Purple Eggplant’s Peel (Solanum melongena L) to Reduce Blood Sugar Levels in White Rat (Rattus norvegicus) Induced by Sucrose

2017 ◽  
Vol 6 (2) ◽  
pp. 14
Author(s):  
Nugroho Eko Wirawan Budianto ◽  
Hairullah Hairullah
Keyword(s):  
Blood Sugar ◽  
Solanum Melongena ◽  
Ethanol Extract ◽  
Male Rats ◽  
Flavonoid Compound ◽  
White Rats ◽  
Significant Difference ◽  
Oral Sucrose ◽  
Group 2 ◽  
Sugar Levels

Acarbose is an oligosaccharide human made who is a competitive inhibitor acarbose and can inhibit the action of the enzyme α-glucosidase, causing a slowdown in the digestion of carbohydrate compounds. Purple eggplant skin is included in the flavonoid compound. Purple eggplant  skin as well as an antioxidant that can control blood glucose levels and prevent complications of diabetes. Purple eggplant skin contains flavonoid kaempferol and has the effect of inhibiting the enzyme alpha-glucosidase. Based on these researchers want to know the difference in effectiveness between the ethanol extract of purple eggplant skin  and acarbose to decrease blood sugar levels of mice. The study population using white rats with a total sample size of 30 rats. Research data analysis using One Way Anova Test. Methods: Male rats strain Wistar were divided into 5 groups. 1) Control (CMC Na 1 ml io), 2) Group 1  (The ethanol extract of 50 mg / kg in the CMC Na io),  3) Group 2  (The ethanol extract 100 mg / kg in CMC Na io), 4) Group 3  (the ethanol extract 200 mg / kg in the CMC Na io), 5) Group 4 (Acarbose 4.5 mg / kg in distillated water io). The result showed ethanol extract of purple eggplant skin in group 2 showed the best effectiveness in lowering blood sugar levels of white rats by oral sucrose injection. And there is no significant difference between the effectiveness of the ethanol extract of purple eggplant skin and acarbose in lowering blood sugar levels of white rats by oral sucrose injection.

scholarly journals UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 572
Author(s):  
Asriyani Samiun ◽  
Edwin De Queljoe ◽  
Irma Antasionasti
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Male Rats ◽  
Test Material ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain ◽  
One Way Anova ◽  
Vernonia Cinerea ◽  
Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit  (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit  setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN EFEKTIVITAS EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS JANTAN GALUR WISTAR HIPERURISEMIA

2020 ◽  
Vol 3 (1) ◽  
pp. 29-37
Author(s):  
Bunga Rimta Barus
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Weight Group ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Sida Rhombifolia

Sidaguri leaves (Sida rhombifolia L.) Have been shown to contain flavonoid compounds, where they are efficacious as hyperuricemia. This study aims to determine effectiveness ethanol extract of sidaguri leaves as antihyperuricemia in white rats. The study was conducted using the method of potassium oxanate induction and measured using glucometers. 15 animals were divided into 5 groups, namely negative control, positive control, group I combination of ethanol extract of sidaguri leaves 50 mg / kg body weight, group II ethanol extract of sidaguri leaves 100 mg / kg body weight, group III ethanol extract of sidaguri leaves 200 mg / kg body weight for 5 hours. Examination of antihyperuricemia effects is done by comparing the uric acid levels value of the rats before and after treatment. The results of this study indicate that the three dose ethanol extract of sidaguri leaves provide an antihyperuricemia effect and based on one-way Anova, Tuckey and LSD data analysis, shows that the dose of 50 mg / kg body weight is effective as antihyperuricemia and does not have a significant difference (p> 0.05). The conclusion of this study is that the dose is the most effective in decreases uric acid levels in white rats, which is a dose of 200 mg / kg body weight because it mostly decreased uric acid levels.

scholarly journals Potensi Rumput Laut (Eucheuma cottonii J.Agardh) Terhadap Nefropati Diabetik Tikus Putih Jantan (Rattus norvegicus)

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Joni Tandi ◽  
Niluh Puspita Dewi ◽  
Resky Chandra Wirawan ◽  
Megawati R. Surat
Keyword(s):  
Blood Glucose ◽  
Kidney Tissue ◽  
Ethanol Extract ◽  
Seaweed Extract ◽  
Average Value ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Eucheuma Cottonii

ABSTRACT   This study aims to determine the effect of a multilevel dose of ethanol extract of seaweed (Eucheuma cottonii J. Agardh) on the decreasing blood glucose levels and kidney tissue regeneration of hypercholesterolemia-diabetic male white rats. 24 rats were divided into 6 groups, namely groups 1-3 (normal control, negative control, and positive control/Glibenclamide, respectively) and groups 3-6 (seaweed extract doses of 100, 200, and 400 mg/kg BW per oral, respectively). Rats were induced by high fat diet and Streptozotocin. Histopathological picture of the kidney was observed by HE staining using 400 magnifications. Data scoring of the level of kidney damage were analyzed by One Way ANOVA at a 95% confidence level and a Least Significant Difference (LSD) test was conducted. Seaweed ethanol extract at a dose of 100 mg/kg BW was effective in reducing blood glucose levels with an average value of 122.25 mg/dL and effective in regenerating the kidney tissue of male white rats with an average value of damage of 1.9. Therefore, seaweed extract has potency to be developed and further investigated as an antidiabetic agent.

scholarly journals Features of angioarchitectonics and structure of the aortic wall of white rats in the early stages of experimental diabetes mellitus

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 167-174
Author(s):  
M.N. Tsytovskyi ◽  
M.V. Logash ◽  
I.I. Savka ◽  
G.M. Dmytriv
Keyword(s):  
Diabetes Mellitus ◽  
Aortic Wall ◽  
Experimental Diabetes ◽  
White Male ◽  
Male Rats ◽  
White Rats ◽  
Significant Difference ◽  
Streptozotocin Induced Diabetes ◽  
Atherosclerotic Cardiovascular Diseases ◽  
Imagej Software

Background. Atherosclerotic cardiovascular diseases, as well as coronary heart disease, cerebrovascular disease, and peripheral artery disease (probably caused by atherosclerosis), are the leading cause of disability and mortality in people with diabetes. Objective. The purpose of our study was to determine the histostructural features and morphometric analysis of the components of the aortic wall and its hemomicrocirculatory bed after 2 and 4 weeks of streptozotocin-induced diabetes mellitus. Methods. The samples for the histology were the sections of the wall of the ascending part, the aortic arch, and the descending part of the aorta of 26 mature white male rats weighing 100 - 160 g. For morphometric examination, a series of images of the aortic wall was taken using a Meiji MT4300 LED microscope with an x40 objective, x10 eyepiece. Results and conclusion. The measurements were carried out using the ImageJ software. The development of micro- and macroangiopathies in experimental animals with 8-week streptozotocin-induced diabetes mellitus was histologically proved. Statistical analysis revealed a significant difference of all morphometric parameters of both - the components of the aortic wall and the vessels of its hemomicrocirculatory bed after 4 weeks of experimental diabetes in comparison with the norm, control, and the 2-nd week of the experiment. An explicit dependency of the severity of destructive changes in the wall of the aorta and links of its hemomicrocirculatory bed of vessels on the duration of the experiment was determined.

scholarly journals Anti-inflammatory Activity of Ethanol Extract of Lotus (Nelumbo nucifera G.) Seed Against White Male Rats Using Paw Edema Method

2021 ◽  
Vol 11 (4) ◽  
pp. 1-4
Author(s):  
Khairani Fitri ◽  
Tetty Noverita Khairani ◽  
Kristin Tiurma Sianturi ◽  
Leny Leny ◽  
Ihsanul Hafiz
Keyword(s):  
White Male ◽  
Ethanol Extract ◽  
Nelumbo Nucifera ◽  
Inflammatory Activity ◽  
Male Rats ◽  
Control Group ◽  
Paw Edema ◽  
White Rats ◽  
Activity Test ◽  
Anti Inflammatory

The purpose of this study was to determine the anti-inflammatory activity test of ethanol extract of lotus seeds in rats induced by carrageenan. The research method used was experimental. The object used in this study was male white rats. Observations were made for 6 hours and then the data were analyzed using the one way analysis of variance (ANOVA) test. The anti-inflammatory activity test was carried out on the test animals which were divided into 5 groups, 3 each with the suspension test group with a dose of 200, 300, 400 mg/kg bw, the negative control group was the CMC Na 1% suspension and the positive control was diclofenac Na 2, 25 mg/kg bw. The results showed that the ethanol extract of the seeds of lotus (Nelumbo nucifera G) was proven to have an anti-inflammatory effect on carrageenan-induced male white rats at doses of 200, 300 and 400 mg/kg BW with the percentage value of inflammation inhibition at the 6th hour was 65.79, 74.65 and 80.77%, with the most effective dose in reducing anti-inflammatory drugs being 400 mg/kg bw. The ethanol extract of the seeds of lotus (Nelumbo nucifera) has anti-inflammatory activity. Keywords: anti-inflammatory activity, Nelumbo nucifera, paw edema

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.)

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 595
Author(s):  
Anissa Sedu ◽  
Edwin De Queljoe ◽  
Julianri Sari Lebang
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Test Dose ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
White Rats ◽  
One Way Anova ◽  
Wistar Male Rats ◽  
Dpt Vaccine

ABSTRACTStarfruit leaves (Averrhoa Bilimbi L) is a plant that contains tannins and flavonoids. In accordance with previous research it was stated that tannins and flavonoids have antiperetic activity. This study aims to determine whether the ethanol extract of starfruit leaves has an antiperetic effect in wistar male rats (Rattus Norvegicus L) induced by the DPT vaccine. This study used 15 test animals and devided into 5 grops consisting of negative control (cmc 1%), positive control (paracetamol) and the test dose group namely the administration of starfruit leaves extract 3,6 mg/200 grams BW, 7,2 mg/200 grams BW and 14,4 mg/200 grams BW. Each mouse was induced with DPT vaccine doses of 0,2 ml intramuscularly. Temperature measurements were made taken before induction, 2 hours after induction and every 30 minutes for 3 hours. The results showed a decrease in temperature in the 60th minute of starfruit leaves 3,6 mg/200 grams BW, 7,2 mg/200 grams BW and 14,4 mg/200 grams BW. The data obtained were analyzed using one-way ANOVA test and LSD test. Based on the results of the study it can be concluded that the ethanol extract of starfruit leaves 3,6 m, 7,2 mg and 14,4 mg has antiperetic activity in male white rats. Keywords : Averrhoa bilimbi L,Antiperetic,Rattus novergicus L,Vaksin DPTABSTRAK Daun belimbing wuluh (Averrhoa bilimbi L.)  adalah salah satu tanaman yang mengandung zat tanin dan flavonoid. Berdasarkan penelitian-penelitian sebelumnya menyatakan bahwa tanin dan flavonoid mempunyai aktivitas antipiretik. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun belimbing wuluh memiliki efek sebagai antipiretik pada tikus putih jantan (Rattus norvegicus L.) yang di induksikan vaksin DPT. Penelitian ini menggunakan hewan uji sebanyak 15 ekor dan dibagi menjadi 5 kelompok yang terdiri dari kontrol negatif (CMC 1%),  kontrol positif (parasetamol) dan kelompok dosis uji yaitu pemberian ekstrak daun Belimbing wuluh 3,6 mg/200 gram BB, 7,2 mg/200 gram BB, dan 14,4 mg/200 gram  BB. Tiap  tikus diinduksi dengan vaksin DPT sebanyak 0,2 ml secara intramuskular. Pengukuran suhu dilakukan sebelum induksi, 2 jam setelah pemberian induksi dan setiap 30 menit selama 3 jam. Hasil penelitian menunjukkan terjadi penuruan suhu pada menit yang ke 60 ekstrak etanol daun belimbing wuluh 3,6 mg/200 gram BB, 7,2 mg/200 gram BB, dan 14,4 mg/200 gram BB.  Data yang diperoleh dianalisis dengan menggunakan uji ANOVA satu arah dan uji LSD dapat menurunkan suhu rektal tikus. Data yang diperoleh dianalisa menggunakan ANOVA. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol daun belimbing wuluh 3,6 mg, 7,2 mg, dan 14,4 mg mempunyai aktivitas antipiretik pada tikus putih jantan.  Kata Kunci :  Averrhoa bilimbi L., Antipiretik, Rattus norvegicus L,  Vaksin DPT

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