scholarly journals PENGARUH PEMBERIAN EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa-sinensis L.) TERHADAP JUMLAH SPERMATOZOA, BERAT BADAN, DAN BERAT TESTIS TIKUS JANTAN WISTAR (Rattus norvegicus)

2019 ◽  
Vol 19 (1) ◽  
pp. 6
Author(s):  
Nabila S Petta ◽  
Edwin De Queljoe ◽  
Rooije R.H. Rumende
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
The Body ◽  
Male Rats ◽  
White Rats ◽  
Hibiscus Rosa Sinensis ◽  
Randomized Design ◽  
Male Wistar Rats ◽  
Ethanol Extracts

PENGARUH PEMBERIAN EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa-sinensis L.) TERHADAP JUMLAH SPERMATOZOA, BERAT BADAN, DAN       BERAT TESTIS TIKUS JANTAN WISTAR (Rattus norvegicus)ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol kembang sepatu terhadap jumlah spermatozoa tikus jantan wistar (Rattus norvegicus). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan menggunakan 24 ekor tikus putih jantan galur wistar (Rattus norvegicus) yang dibagi atas beberapa kelompok dimana kelompok 1 sebagai kelompok kontrol tanpa perlakuan, kelompok 2, 3 dan 4 sebagai kelompok perlakuan dengan dosis secara berturut-turut 3,6 mg/ml; 7,2 mg/ml; dan 14,4 mg/ml. Perlakuan diberikan secara oral sekali sehari sebanyak 1 ml selama 50 hari sesuai siklus spermatogenesis. Variabel yang diamati yakni jumlah sel spermatozoa, berat badan, dan berat testis. Hasil penelitian ini menunjukkan bahwa ekstrak etanol kembang sepatu dapat menurunkan jumlah sel spermatozoa, serta menyebabkan adanya perbedaan berat badan dan berat testis namun, berdasarkan hasil analisis varians, ekstrak etanol daun kembang sepatu tidak dapat menurunkan jumlah sel spermatozoa, berat badan dan berat testis tikus putih jantan galur wistar (Rattus norvegicus) secara signifikan.Kata Kunci: Sel spermatozoa, Kembang sepatu (Hibiscus rosa-sinensis L.),                    Tikus jantan Wistar (Rattus novergicus) THE INFLUENCE OF THE ETHANOL EXTRACTS  OF GRANTING HIBISCUS (Hibiscus Rosa-sinensis L.) AGAINST THE NUMBER OF SPERMATOZOA, WEIGHT AND THE WEIGHT OF THE TESTES MALE WISTAR RATS (Rattus norvegicus) ABSTRACTThis research’s objective is to know the influence of injecting ethanol extract from a hibiscus into a number of common male rats (Rattus norvegicus). This research uses the approach of complete randomized design (CRD) onto 24 common white rats (Rattus norvegicus) that is divided into groups, where group 1’s approach is control without treatment, groups 2, 3, and 4’s approach is with treatment, with consecutive doses being 3.6 mg/ml; 7.2 mg/ml; and 14.4 mg/ml.  The treatment is induced orally as large as 1cc per day for a total of 50 days following the spermatogenesis cycle. The variables that are being observed are the amount of spermatozoon cells, body weight, and testicle weight. The results of this research indicates that ethanol extract from hibiscuses, from a quantity perspective, can decrease spermatozoon cells, and it may also influence the body weight and testicle weight of the subject, in this case are common rats (Rattus norvegicus) but, from the mathematical results from Analysis Of Variance, ethanol extract from the leaves of a hibiscus cannot decrease the amount of spermatozoon, body weight, and testicle weight  of a common white rat (Rattus norvegicus).Keywords: Spermatozoon Cells, Hibiscus (Hibiscus rosa-sinensis L.), Common (white) rat (Rattus novergicus)

scholarly journals Profile of SGPT and SGOT on Male Rats (Rattus norvegicus) Hyperglycemic After Giving Insulin Leaf Extract (Tithonia diversifolia)

2018 ◽  
Vol 10 (3) ◽  
pp. 519-525
Author(s):  
Rizki Fitrawan Yuneldi ◽  
Tyas Rini Saraswati ◽  
Enny Yusuf Wachidah Yuniwarti
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Liver Weight ◽  
Male Rats ◽  
Hepatosomatic Index ◽  
Tithonia Diversifolia ◽  
White Rats ◽  
Improve Liver Function ◽  
Male Wistar Rats

The leaves of insulin (Tithonia diversifolia (Hemsl.) A. Gray) are native bush plants from Central America. This plant in Indonesia is often called paitan or kembang bulan. The aim of this research was to know the profile of SGPT and SGOT of male white rat (Rattus norvegicus L.) hyperglycemic after giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray). This study used 20 male Wistar rats which were divided randomly into 5 groups of treatment. Those were P0 as a group of rats which was not given alloxan and insulin leaf extract, P1 as a group of rats which was given alloxan without insulin leaf extract, P2 as a group of rats which was given alloxan and glibenclamide 2 mg/Body Weight (BW)/day, P3 as a group of rats which was given alloxan and insulin leaf extract 30 mg/BW/day, P4 as a group of rats which was given alloxan and insulin leaf extract 60 mg/BW/day. Each treatment was repeated as many as 4 replications. The results of this study indicated that there was no significant differences in all variables, namely levels of SGPT, SGOT, liver weight, body weight and Hepatosomatic index (HSI), so it can be concluded that giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray) of 30 mg/BW/day and 60 mg/BW/day could improve liver function of hyperglycemic male white rats (R. norvegicus L.).

scholarly journals UJI AKTIVITAS ANTIHIPERURISEMIA KOMBINASI EKSTRAK ETANOL DAUN SALAM (Syzygium polyanthum (Wight.) Walp) dan TUMBUHAN SURUHAN (Peperomia pellucida (L.) Kunth) PADA TIKUS PUTIH JANTAN (Rattus Norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 581
Author(s):  
Chintia M. Manopo ◽  
Widdhi Bodhi ◽  
Elly J. Suoth
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Ethanol Extracts ◽  
Existing Data

ABSTRACTBay leaves (Syzygium polyanthum (Wight.) Walp) and herbs (Peperomia pellucida (L.) Kunth) are known to contain flavonoids, where flavonoids have been known to have the ability to inhibit the ksantin oxidase, which causes uric acid levels in the blood to drop. This study used a completely randomized design method. This study aims to determine whether the combined ethanol extract of bay leaves (Syzygium polyanthum (Wight.) Walp) and suruhan (Peperomia pellucida (L.) Kunth) has antihyperuricemic activity. There were 15 rats used and divided into 5 groups, namely negative control (CMC 1%), positive control (Allopurinol), and ethanol extracts of bay leaves and herbs with a dose of 50 m, 100 mg, 200 mg. Existing data were analyzed using the ANOVA test and LSD test. The analysis showed that the combined ethanol extract of bay leaves (Syzygium polyanthum (Wight.) Walp) and suruhan (Peperomia pellucida (L.) Kunth) had antihyperuricemic activity against male white rats. Keywords: Syzygium polyanthum (Wight.) Walp) and Peperomia pellucida (L.) Kunth, Antihyperuricemia, Rattus Norvegicus.ABSTRAKDaun salam (Syzygium polyanthum (Wight.) Walp) dan tumbuhan suruhan (Peperomia pellucida (L.) Kunth) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan dalam menghambat ksantin oksidase, yang menyebabkan kadar asam urat di dalam darah turun. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol kombinasi dari daun salam (Syzygium polyanthum (Wight.) Walp ) dan tumbuhan suruhan (Peperomia pellucida (L.) Kunth) memiliki aktivitas antihiperurisemia. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (CMC 1%), kontrol positif (Allopurinol), dan ekstrak etanol daun salam dan tumbuhan suruhan dengan dosis 50 mg, 100 mg, 200 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menunjukkan bahwa ekstrak etanol kombinasi daun salam (Syzygium polyanthum (Wight.) Walp) dan tumbuhan suruhan (Peperomia pellucida (L.) Kunth ) memiliki aktivitas antihiperurisemia terhadap tikus putih jantan.Kata kunci: Syzygium polyanthum (Wight.) Walp) dan  Peperomia pellucida (L.) Kunth, Antihiperurisemia, Rattus Norvegicus

scholarly journals Toxicity assessment of a technical product of the triazole class

2020 ◽  
Vol 99 (11) ◽  
pp. 1276-1279
Author(s):  
Valery N. Rakitskii ◽  
Tatiana M. Epishina ◽  
Elena G. Chkhvirkiya
Keyword(s):  
Body Weight ◽  
The Body ◽  
Male Rats ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
High Biological Activity ◽  
Experimental Animals ◽  
Technical Product ◽  
White Rats ◽  
Toxicological Studies

Introduction. Historically, pesticides are evaluated more strictly from a medical point of view than other chemicals. Since their features, such as deliberate introduction into the environment, the possibility of contact with them by large masses of the population, and the high biological activity determine their potential danger to humans. Purpose of research - study of the biological effect of a technical product derived from triazoles when it is repeatedly ingested orally in mammals (rats), establishment of inactive and active doses, justification of the permissible daily dose (DSD) for humans. Material and methods. In acute experiments, white rats were used, including 6 animals in the group. Tested dose: 500-4000 mg/kg of body weight. A chronic (12 months) experiment was performed on 80 male rats with a bodyweight of 180-190 g at the beginning of the study. Tested doses: 5.0; 16.0 and 55.0 mg/kg of body weight (1 control and 3 experimental animals, 20 individuals each). In the dynamics of the experiment, we observed the condition and behavior of animals, water, and food consumption, recorded the timing of death, changes in body weight, physiological, biochemical, and hematological indices. Results. Indices of the acute oral toxicity on the studied product LD50 male rats were 2250 ± 483 mg/kg body weight. The dose of 5.0 mg / kg of body weight was not found to cause significant changes in all studied indices. The doses of 16.0 and 55.0 mg/kg of body weight had a polytropic effect on the body in experimental animals. Discussion. The studied product for the acute oral toxicity refers to low-hazard compounds, the doses of 16.0 and 55.0 mg/kg of body weight has a polytropic effect on the mammalian body, causing changes in carbohydrate, lipid, and lipoprotein metabolism in the body of rats - was accepted as acting. The dose of 5.0 mg / kg of body weight, when administered in rats, there are no changes in all the studied parameters throughout the experiment, is accepted as invalid. Based on the inactive dose-5.0 mg/kg of body weight and taking into account the reserve factor of 100, we have scientifically justified DSD for a person at the level of 0.05 mg/kg. Summary. The conducted sanitary and Toxicological studies indicate the need to assess the toxicity of new technical products to the mammalian body, to increase the reliability of the developed hygiene standards in environmental objects and food products.

scholarly journals Ulva lactuca Linnaeus Potentially Promotes Reproductive Indices and Depressive-like Behavior of Hypertriglyceridemia Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

2020 ◽  
Vol 5 (3) ◽  
pp. 228
Author(s):  
Anggoro Chandra Yulistiyanto ◽  
Mayumi Hersasanti ◽  
Rahadian Yudo Hartantyo ◽  
Laksmindra Fitria ◽  
Abdul Razaq Chasani ◽  
...  
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Gonadosomatic Index ◽  
Ulva Lactuca ◽  
The Body ◽  
Physiological Functions ◽  
Testosterone Concentration ◽  
Natural Treatment ◽  
Male Wistar Rats

Excessive consumption of fatty foods can lead to hyperlipidemia, which is often coupled with hypertriglyceridemia (HTG), a condition where blood plasma triglyceride (TG) levels elevated beyond normal levels. This condition may disturb physiological functions of the body, such as reproductive functions, and other physiological imbalances leading to chronic stress and depression. Ulva lactuca is a potential natural treatment for HTG, as it contains various nutrients to aid physiological functions. This seaweed also has high levels of Cd, which can increase depression. Therefore, research on the potential benefits of U. lactuca should be followed by an investigation of its health risks. This research aimed to examine the effects of HTG and treatment with U. lactuca on reproduction and depressive-like behavior of male Wistar rats (Rattus norvegicus Berkenhout, 1769). The data collected in this research include body weight, serum TG concentration, gonadosomatic index (GSI), serum testosterone concentration using competitive ELISA, and depressive-like behaviors assessed using the Forced Swim Test (FST) and Open Field Test (OFT). Data were analyzed using One-Way ANOVA followed by DMRT, independent- and paired-samples t-test, and Kruskal-Wallis H test with a significance value of α=0.05. Body weight, serum TG and testosterone concentration, GSI, and depressive-like behaviors were increased by the HTG condition. Ulva lactuca at the1500 mg/kg BW/day did not significantly affect body weight, testosterone concentration, and depressive-like behaviors of HTG rats. Meanwhile, this treatment significantly increased the GSI and depressive-like behaviors of healthy rats. These results suggest that Ulva lactuca treatment not only enhances gonad growth and development but also increases depressive-like behaviors.

scholarly journals UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 572
Author(s):  
Asriyani Samiun ◽  
Edwin De Queljoe ◽  
Irma Antasionasti
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Male Rats ◽  
Test Material ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain ◽  
One Way Anova ◽  
Vernonia Cinerea ◽  
Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit  (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit  setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

Diuretic activity of ethanol extract of Mirabilis jalapa (Linn.) leaf in normal male Wistar rats

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Musa T. Yakubu ◽  
Adenike M Oyagoke ◽  
Luqman A. Quadri ◽  
Anthonia O. Agboola ◽  
Hussein O.B. Oloyede
Keyword(s):  
Body Weight ◽  
Carbonic Anhydrase ◽  
Urine Volume ◽  
Ethanol Extract ◽  
The Body ◽  
Male Rats ◽  
Normal Male ◽  
Diuretic Activity ◽  
Urine Ph ◽  
Mirabilis Jalapa

Background: Ethanol extract of Mirabilis jalapa leaf (EEMJL) has been used in the folk medicine of Nigeria as diuretics without any scientific evidence.Aim: Ethanol extract of Mirabilis jalapa leaf at 200, 400 and 600 mg/kg body weight was investigated for diuretic activity in male Wistar rats.Setting: Fresh leaf of M. jalapa was collected from a farmland at the Alanamu area in Ilorin, Kwara State, Nigeria, authenticated and processed for the study.Methods: Thirty male rats (231.50 g ± 13.51 g) were assigned into five groups (A–E) of six rats each. Rats in group A (control) received 1.0 mL of physiological saline (the vehicle). Animals in groups B (positive control), C, D and E received 1.0 mL equivalent to 100 mg/kg body weight of furosemide, 200, 400 and 600 mg/kg body weight of EEMJL, respectively. All administrations were done by oral gavage. The animals were monitored for indicators of diuresis for 5 h using standard methods.Results: Ethanol extract of Mirabilis jalapa leaf dose-dependently increased (p < 0.05) urine volume, urine concentrations of Na+, K+ and Cl− and decreased (p < 0.05) the body weight of the animals. Ethanol extract of Mirabilis jalapa leaf increased the urine pH, saliuretic activity, saliuretic index, Na+ index, K+ index, Cl− index, diuretic action (diuretic index), kaliuretic index, Lipschitz value and percentage saline load excreted, whereas the latency of urination, natriuretic index, carbonic anhydrase inhibitory activity and carbonic anhydrase inhibition index were decreased. The EEMJL treatment-related changes in these parameters were essentially similar to those of the furosemide-treated animals.Conclusion: This study has thus validated diuretic activity of M. jalapa leaf with the 600 mg/kg body weight of EEMJL being the most effective.

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.)

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 595
Author(s):  
Anissa Sedu ◽  
Edwin De Queljoe ◽  
Julianri Sari Lebang
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Test Dose ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
White Rats ◽  
One Way Anova ◽  
Wistar Male Rats ◽  
Dpt Vaccine

ABSTRACTStarfruit leaves (Averrhoa Bilimbi L) is a plant that contains tannins and flavonoids. In accordance with previous research it was stated that tannins and flavonoids have antiperetic activity. This study aims to determine whether the ethanol extract of starfruit leaves has an antiperetic effect in wistar male rats (Rattus Norvegicus L) induced by the DPT vaccine. This study used 15 test animals and devided into 5 grops consisting of negative control (cmc 1%), positive control (paracetamol) and the test dose group namely the administration of starfruit leaves extract 3,6 mg/200 grams BW, 7,2 mg/200 grams BW and 14,4 mg/200 grams BW. Each mouse was induced with DPT vaccine doses of 0,2 ml intramuscularly. Temperature measurements were made taken before induction, 2 hours after induction and every 30 minutes for 3 hours. The results showed a decrease in temperature in the 60th minute of starfruit leaves 3,6 mg/200 grams BW, 7,2 mg/200 grams BW and 14,4 mg/200 grams BW. The data obtained were analyzed using one-way ANOVA test and LSD test. Based on the results of the study it can be concluded that the ethanol extract of starfruit leaves 3,6 m, 7,2 mg and 14,4 mg has antiperetic activity in male white rats. Keywords : Averrhoa bilimbi L,Antiperetic,Rattus novergicus L,Vaksin DPTABSTRAK Daun belimbing wuluh (Averrhoa bilimbi L.)  adalah salah satu tanaman yang mengandung zat tanin dan flavonoid. Berdasarkan penelitian-penelitian sebelumnya menyatakan bahwa tanin dan flavonoid mempunyai aktivitas antipiretik. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun belimbing wuluh memiliki efek sebagai antipiretik pada tikus putih jantan (Rattus norvegicus L.) yang di induksikan vaksin DPT. Penelitian ini menggunakan hewan uji sebanyak 15 ekor dan dibagi menjadi 5 kelompok yang terdiri dari kontrol negatif (CMC 1%),  kontrol positif (parasetamol) dan kelompok dosis uji yaitu pemberian ekstrak daun Belimbing wuluh 3,6 mg/200 gram BB, 7,2 mg/200 gram BB, dan 14,4 mg/200 gram  BB. Tiap  tikus diinduksi dengan vaksin DPT sebanyak 0,2 ml secara intramuskular. Pengukuran suhu dilakukan sebelum induksi, 2 jam setelah pemberian induksi dan setiap 30 menit selama 3 jam. Hasil penelitian menunjukkan terjadi penuruan suhu pada menit yang ke 60 ekstrak etanol daun belimbing wuluh 3,6 mg/200 gram BB, 7,2 mg/200 gram BB, dan 14,4 mg/200 gram BB.  Data yang diperoleh dianalisis dengan menggunakan uji ANOVA satu arah dan uji LSD dapat menurunkan suhu rektal tikus. Data yang diperoleh dianalisa menggunakan ANOVA. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol daun belimbing wuluh 3,6 mg, 7,2 mg, dan 14,4 mg mempunyai aktivitas antipiretik pada tikus putih jantan.  Kata Kunci :  Averrhoa bilimbi L., Antipiretik, Rattus norvegicus L,  Vaksin DPT

scholarly journals Effect of Ethanol Extract of Mangosteen Peel on Serum Malondialdehyde and Leukotriene B4 Levels in Male White Rats (Rattus norvegicus) Wistar Strain Exposed with Electric Cigarette Smoke

2021 ◽  
Vol 56 (4) ◽  
pp. 283
Author(s):  
Jemima Lewi Santoso ◽  
Harianto Notopuro ◽  
Ema Qurnianingsih
Keyword(s):  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Leukotriene B4 ◽  
The Body ◽  
Control Group ◽  
Test Results ◽  
White Rats ◽  
Mangosteen Peel ◽  
Significant Difference

This study aims to analyze the administration of mangosteen peel ethanol extract at different doses can reduce levels of malondialdehyde (MDA) and leukotriene B4 (LTB4) serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke. Electric cigarette induces mitochondrial ROS production and triggers oxidative stress. Antioxidants are needed by the body if there are many free radicals, for example mangosteen peel. This laboratory experimental study uses a randomized post test only control group design. Thirty male white rats were divided into 5 groups randomly (KK, KR, P1, P2, P3). KK given 0.5% Na-CMC, KR given smoke and 0.5% Na-CMC, P1, P2, P3 given smoke and mangosteen peel ethanol extract 100, 200, 300 mg / kg weight. Data were tested for normality and homogeneity tests. Data distribution was found not normal. Data were tested by Kruskal-Wallis Test and Mann-Whitney. Kruskal-Wallis Test results showed significant differences in MDA and LTB4 levels in each experimental group (KS, KR, P1, P2 and P3), p <0.05. Mann-Whitney test results showed a significant difference in the MDA levels of the KR against P3 and in the LTB4 level of the KR against P2 (p <0.05). The conclusion of the study was the administration of mangosteen peel ethanol extract can reduce MDA and LTB4 levels serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke.

scholarly journals The Effect of Mangosteen Rind Extract (Garcinia mangostana L.) Toward Stress Oxidative Parameter, Leukocytes, Leukocytes Type Counts on Male Rats (Rattus norvegicus) with Excessive Physical Activity

2020 ◽  
Vol 8 (A) ◽  
pp. 904-909
Author(s):  
Rika Nailuvar Sinaga ◽  
Zulaini Zulaini
Keyword(s):  
Oxidative Stress ◽  
Physical Activity ◽  
Rattus Norvegicus ◽  
Leukocyte Count ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Average Percentage ◽  
White Rats

BACKGROUND: Excessive and heavy physical activity could lead to the oxidative stress in the body. This condition can affect several blood parameters such as malondialdehyde (MDA), glutathione peroxidase (GPx), leukocytes, and its type counts. One way to overcome the effects of oxidative stress due to strenuous physical activity is by giving natural antioxidants. Mangosteen rind (Garcinia mangostana L.) contains considerable antioxidants, namely, xanthones and procyanidin. AIM: This research aimed to investigate the effect of mangosteen rind extract (MRE) on oxidative stress parameters, leukocyte count, and leukocyte count in male rats (Rattus norvegicus) that received excessive physical activity. METHODS: This study was purely experimental research with a post-test only control group design, using 29 white rats (R. norvegicus) as experimental animals, which were divided into four groups. The first group (P1) received distilled water and the other group received MRE at a dose of 50 mg/KgBW (Body Weight) (P2), 100 mg/KgBW (P3), and 200 mg/KgBW (P4) for 3 weeks, respectively. Swimming as a physical activity was carried out 3 times a week in all groups. Examination of leukocyte count and species was carried out on the last day of treatment after the rats swam to fatigue. RESULTS: MDA levels did not decrease significantly while GPx activity increased significantly. Moreover, the average leukocytes and lymphocytes showed a decrease but not significant. A significant decrease in the percentage of neutrophils and basophils was obtained by P4 group compared to others. In addition, a significant decrease in the average percentage of eosinophils for P3 and P4 groups was obtained. Furthermore, P3 and P4 group exhibit significant increase of the average percentage of monocytes. CONCLUSION: MRE as an antioxidant affects the levels of neutrophils, basophils, eosinophils, and monocytes but not in MDA, GPx, leukocytes, and lymphocytes.

Sign in / Sign up

Export Citation Format

Share Document