Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers

ABSTRACT Aim To compare the antimicrobial activity of the chemical substances—70% isopropyl alcohol, 2% glutaraldehyde (GTA) and 0.25% peracetic acid (PAA) in disinfecting orthodontic pliers contaminated in vitro with Streptococcus mutans, Staphylococcus aureus and Candida albicans. Materials and methods Distal end cutter pliers were divided into five groups: group 1 (negative control—sterilized pliers), group 2 (positive control—sterilized plier, subsequently contaminated), group 3 (disinfected with 70% isopropyl alcohol, friction method), group 4 (disinfected with 2% GTA, immersion method for 30 minutes), group 5 (disinfected with 0.25% peracetic acid (PAA), immersion method for 10 minutes). After the pliers were treated with one disinfectant and submitted to microbiological evaluation (by counting colony forming units), they were submitted to the same cleansing, sterilizing and contaminating processes, and were used in the following groups (crossover and washout study). The two-factor analysis of variance (ANOVA) test, followed by the Tukey test, was used to compare the groups. Results The results showed that there was no statistically significant difference between the three tested disinfectants. Conclusion Although there were no statistically significant differences between the disinfectants, the chemical agents 2% glutaraldehyde and 0.25% PAA were effective in inhibiting the growth of the three microorganisms tested; however, 70% isopropyl alcohol was unable to completely eliminate S. aureus. Clinical significance The chemical substances 2% glutaraldehyde and 0.25% PAA completely eliminated the microorganisms tested. How to cite this article Carvalho MRA, dos Santos da Silva MA, de Sousa Brito CAR, Campelo V, Kuga MC, Tonetto MR, De Jesus Tavarez RR, Bandéca MC, Pinzan-Vercelino CRM. Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers. J Contemp Dent Pract 2015;16(8):619-623.

Download Full-text

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

Download Full-text

Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

Revista de Biología Tropical ◽

10.15517/rbt.v66i4.33140 ◽

2018 ◽

Vol 66 (4) ◽

pp. 1519

Author(s):

Mailen Ortega Cuadros ◽

Adriana Patricia Tofiño Rivera ◽

Luciano Jose Merini ◽

Maria Cecilia Martinez Pabon

Keyword(s):

Antimicrobial Activity ◽

Dental Caries ◽

Essential Oil ◽

Streptococcus Mutans ◽

Human Fibroblasts ◽

Positive Control ◽

Negative Control ◽

Cymbopogon Citratus ◽

Significant Difference ◽

Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

Download Full-text

Evaluating Dentin Surface Treatments for Resin-Modified Glass Ionomer Restorative Materials

Operative Dentistry ◽

10.2341/12-162-l ◽

2013 ◽

Vol 38 (4) ◽

pp. 429-438 ◽

Cited By ~ 16

Author(s):

TA Imbery ◽

A Namboodiri ◽

A Duncan ◽

R Amos ◽

AM Best ◽

...

Keyword(s):

Testing Machine ◽

In Vitro Study ◽

Positive Control ◽

Negative Control ◽

Surface Treatments ◽

Band Matrices ◽

Dentin Surface ◽

Occlusal Surfaces ◽

Significant Difference

SUMMARY This in vitro study evaluated the effect of six surface treatments on the shear bond strength of three resin-modified glass ionomers (RMGIs) to dentin. Occlusal surfaces of caries-free third molars were reduced to expose only dentin. Surface treatments were smear layer intact (negative control), Cavity Conditioner, EDTA, Ketac Primer, Self Conditioner, and etching with 35% phosphoric acid followed by the application of Optibond Solo Plus. Filtek Z250 composite resin bonded with Optibond Solo Plus served as a positive control. Conditioning agents were used according to the manufacturers' instructions. After surface treatments, Fuji II LC, Riva LC, Ketac Nano, and Filtek Z250 were placed in copper-band matrices 5 mm in diameter and 2 mm in height and were light-cured for 20 seconds. Specimens were stored in 100% humidity for 24 hours, after which they were placed in deionized water for 24 hours at 37°C. They were then tested under shear forces in an Instron Universal Testing Machine at a crosshead speed of 0.5 mm/min. A two-way analysis of variance and Tukey honestly significant difference statistical analyses (p<0.05) indicated significant interaction between RMGIs and conditioning agents. Acid etching followed by Optibond Solo Plus provided highest bond strengths for all three RMGIs, which were not statistically different from the positive control.

Download Full-text

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

The International Journal of Artificial Organs ◽

10.5301/ijao.5000566 ◽

2017 ◽

Vol 40 (2) ◽

pp. 82-87 ◽

Cited By ~ 1

Author(s):

Faika Y. Abdelmegid ◽

Fouad S. Salama ◽

Waleed M. Al-Mutairi ◽

Saud K. Al-Mutairi ◽

Sultan O. Baghazal

Keyword(s):

Primary Teeth ◽

In Vitro Study ◽

Class Ii ◽

Positive Control ◽

Negative Control ◽

The Other ◽

Control Groups ◽

Significant Difference ◽

The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

Download Full-text

Cleaved CD31 as a target for in vivo molecular imaging of inflammation

Scientific Reports ◽

10.1038/s41598-019-56163-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Jonathan Vigne ◽

Sylvie Bay ◽

Rachida Aid-Launais ◽

Guillaume Pariscoat ◽

Guillaume Rucher ◽

...

Keyword(s):

Molecular Imaging ◽

Molecular Form ◽

Positive Control ◽

Negative Control ◽

Stability Study ◽

Biodistribution Studies ◽

Wide Range ◽

Significant Difference

AbstractThere is a need for new targets to specifically localize inflammatory foci, usable in a wide range of organs. Here, we hypothesized that the cleaved molecular form of CD31 is a suitable target for molecular imaging of inflammation. We evaluated a bioconjugate of D-P8RI, a synthetic peptide that binds all cells with cleaved CD31, in an experimental rat model of sterile acute inflammation. Male Wistar rats were injected with turpentine oil into the gastrocnemius muscle two days before 99mTc-HYNIC-D-P8RI (or its analogue with L-Proline) SPECT/CT or [18F]FDG PET/MRI. Biodistribution, stability study, histology, imaging and autoradiography of 99mTc-HYNIC-D-P8RI were further performed. Biodistribution studies revealed rapid elimination of 99mTc-HYNIC-D-P8RI through renal excretion with almost no uptake from most organs and excellent in vitro and in vivo stability were observed. SPECT/CT imaging showed a significant higher 99mTc-HYNIC-D-P8RI uptake compared with its analogue with L-Proline (negative control) and no significant difference compared with [18F]FDG (positive control). Moreover, autoradiography and histology revealed a co-localization between 99mTc-HYNIC-D-P8RI uptake and inflammatory cell infiltration. 99mTc-HYNIC-D-P8RI constitutes a new tool for the detection and localization of inflammatory sites. Our work suggests that targeting cleaved CD31 is an attractive strategy for the specific in vivo imaging of inflammatory processes.

Download Full-text

In Vitro Evaluation of the Efficacy of Different Over-the-Counter Products on Tooth Whitening

Brazilian Dental Journal ◽

10.1590/0103-64402013x0111 ◽

2015 ◽

Vol 26 (4) ◽

pp. 373-377 ◽

Cited By ~ 6

Author(s):

Muhammet Karadas ◽

Zeynep Yesil Duymus

Keyword(s):

Repeated Measures ◽

Positive Control ◽

Negative Control ◽

Carbamide Peroxide ◽

Tooth Whitening ◽

Over The Counter ◽

Color Changes ◽

Significant Difference ◽

Cielab System

<p>This <italic>in vitro</italic> study evaluated the whitening effect of four different over-the-counter products compared with a 10% carbamide peroxide (CP) bleaching gel. Specimens obtained from bovine incisors were stained in a tea solution and randomly divided into six groups according to the product used (n=10): CT: conventional toothpaste (negative control); CWT: Crest 3D White toothpaste; CWR: Crest 3D White mouth rinse; CWS: Crest 3D White strips; DW: Dazzling White (paint-on gel); and OP: Opalescence PF 10% CP (at-home bleaching gel, positive control). Over-the-counter products and 10% CP were used according to the manufacturer's recommendations. Color measurements were performed according to the CIELab system using spectrophotometer after staining, and after 4 and 8 weeks of the treatment procedure. The ∆E, ∆L, ∆a, and ∆b values were calculated. The data obtained were analyzed by repeated-measures ANOVA. Tukey's test for multiple comparisons was applied (p<0.05). All groups, except CWT, were effective in tooth whitening in comparison with conventional toothpaste. There was no significant difference in tooth whitening between CWT and CT (p=0.93). CWS, DW and OP groups presented significantly higher color changes than the CWR group. DW and CWS showed similar tooth whitening to OP.</p>

Download Full-text

Protective Effect of Whole and Fat-Free Fluoridated Milk, Applied before or after Acid Challenge, against Dental Erosion

Caries Research ◽

10.1159/000444024 ◽

2016 ◽

Vol 50 (2) ◽

pp. 111-116 ◽

Cited By ~ 4

Author(s):

Luiza P.S. Cassiano ◽

Senda Charone ◽

Juliana G. Souza ◽

Ligia C. Leizico ◽

Juliano P. Pessan ◽

...

Keyword(s):

Dental Erosion ◽

Positive Control ◽

Negative Control ◽

Nacl Solution ◽

Time Of Application ◽

Whole Milk ◽

Significant Difference ◽

Bovine Enamel ◽

Protective Treatment

This study analysed in vitro the effect of milk against dental erosion, considering three factors: the type of milk (bovine whole/fat-free), the presence of different fluoride concentrations and the time of application (before/after erosive challenge). Bovine enamel (n = 15/group) and root dentine (n = 12/group) specimens were submitted to the following treatments: after the first erosive challenge - 0.9% NaCl solution (negative control), whole milk with 0, 2.5, 5.0 and 10.0 ppm F, fat-free milk with 0, 2.5, 5.0 and 10.0 ppm F, and 0.05% NaF solution (positive control); before the first erosive challenge - whole milk with 0, 2.5, 5.0 and 10.0 ppm F, fat-free milk with 0, 2.5, 5.0 and 10.0 ppm F, and 0.05% NaF solution (positive control). Specimens were submitted to demineralisation-remineralisation regimes 4 times/day for 5 days. The response variables were enamel and dentine loss (in micrometres). Data were analysed using Kruskal-Wallis/Dunn's test (p < 0.05). For enamel, whole milk containing 10 ppm F, applied before the erosive challenge, was the most protective treatment, but with no significant difference compared with the same treatment carried out after the erosive challenge. For dentine, whole fluoridated milk (all concentrations, after), fat-free 10 ppm F milk (after, before) and whole milk with or without F (except 2.5 ppm F, all before) significantly reduced dentine erosion. It seems that the presence of fluoride, especially at 10 ppm, is the most important factor in reducing dental erosion.

Download Full-text

MAT, a Novel Polyherbal Aphrodisiac Formulation, Enhances Sexual Function and Nrf2/HO-1 Pathway While Reducing Oxidative Damage in Male Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/8521782 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Kazim Sahin ◽

Mehmet Tuzcu ◽

Cemal Orhan ◽

Hasan Gencoglu ◽

Nurhan Sahin ◽

...

Keyword(s):

Sexual Function ◽

Treated Group ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Mucuna Pruriens ◽

Tribulus Terrestris ◽

Sexual Hormones ◽

Significant Difference ◽

Group 5

Mucuna pruriens, Ashwagandha, and Tribulus terrestris are known as the enhancers for sexual health, functional activities, vitality, and longevity. These herbs had been widely used in the Ayurveda medicine as aphrodisiacs through the ages, and their efficacy was also verified separately in our previous publication. Therefore, the aim of this study was to determine the effects of Mucuna, Ashwagandha, and Tribulus complexes on sexual function in rats. Twenty-eight male rats allocated to four groups as follows: (i) negative control (C); (ii) positive control or sildenafil citrate treated group (5 mg/kg) (S); (iii) MAT1 (combination of 10 mg Mucuna (M) + 10 mg Ashwagandha (A) + 10 mg Tribulus (T)/kg BW); (iv) MAT 2 (20 mg Mucuna + 20 mg Ashwagandha + 20 mg Tribulus/kg BW). There was no significant difference found between the MAT1 and MAT2 groups while they showed significantly increased testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels when compared to the negative control. Significant increases in Nrf2/HO1 levels and decreases in NF-κB were detected in MAT groups similar to the decrease in serum and testis malondialdehyde (MDA) levels as compared to both controls. The sperm motility, count, and rate also significantly improved in both MAT groups, while ALT, AST, creatinine, ALP, and urea levels did not change in any of the groups. Oral consumption of MATs combination in male rats resulted in inhibition of NF-κB and MDA and also increased sex hormones with Nrf2-mediated HO-1 induction. MAT combinations may improve sexual functions by increasing levels of sexual hormones and regulation of NF-κB and Nrf2/HO-1 signaling pathways.

Download Full-text

The antifungal effect of roselle calyx extract on Trichophyton rubrum growth in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f100103 ◽

2012 ◽

Vol 10 (1) ◽

pp. 17-22

Author(s):

SITA AULIA SARI ◽

RUBEN DHARMAWAN ◽

PARAMASARI DIRGAHAYU

Keyword(s):

Trichophyton Rubrum ◽

Positive Control ◽

Negative Control ◽

Antifungal Effect ◽

Inhibition Effect ◽

Extract Concentration ◽

Significant Difference ◽

Object Of Study ◽

One Way Anova

Sari SA, Dharmawan R, Dirgahayu P. 2012. The antifungal effect of roselle calyx extract on Trichophyton rubrum growth in vitro. Biofarmasi 10: 17-22. Dermatophytosis is a fungal infection on skin that one of them caused by Trichophyton rubrum. Dermatophytosis treatment by using chemical drugs has many shortcomings, such as a high cost and a drug resistance. Roselle (Hibiscus sabdariffa L.) calyx content was flavonoid, which have an antifungal effect. Flavonoids on roselle calyx include anthocyanin, gossypeptin (hexahydroxyflavone) 3-glucoside, flavonol glucoside hibiscritin, flavonoid gossypeptin, delphinidine 3-monoglucoside, cyanidin 3-monoglucoside. The objective of this study was to determine the effect of roselle calyx on Trichophyton rubrum growth in vitro. The study was performed as an experimental laboratory. The object of study was T. rubrum. The sample of T. rubrum colonies in this study was taken by a random sampling. The study used T. rubrum colonies on seven Sabouraud Dextrose Agar plates. Each plate had four holes. Each hole was filled by aquadest as a negative control, fluconazole 25 Âµg/mL as a positive control, and various roselle calyx extract concentrations (10%, 20%, 30%, 40% and 50%). The plates were incubated in an incubator with a temperature of 25oC for 7 days and measured for the diameter of roselle calyx extract inhibition effect. The data were collected and analyzed by One-way ANOVA and Least Significance Difference (LSD) tests on SPSS 16.0 for Windows. The result of One-way ANOVA test showed that there was a difference of inhibition diameter mean among all of the various roselle calyx extract concentration groups (p<0.05). The diameter of roselle calyx extract inhibition effect increased for each concentration up to 50%. The inhibition diameter of positive control compared to 20% roselle calyx extract concentration had no a significant difference. The study was concluded that roselle calyx extract has an antifungal effect to T. rubrum growth in vitro.

Download Full-text

Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v3 ◽

2020 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs. Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm. Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively. Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

Download Full-text