Up-regulated YKL-40 is associated with poor prognosis of HCC patients with hepatitis B related cirrhosis

IntroductionTo investigate clinical significance of YKL-40 in hepatocellular carcinoma (HCC) patients with hepatitis B (HBV) related cirrhosis.Material and methodsThe present prospective observational study included 129 cases of HCC patients. Besides, 152 patients with only hepatitis B related cirrhosis and 110 HCC patients with no cirrhosis were also enrolled during the same period. Additionally, 100 healthy individuals were enrolled as control. Serum YKL-40 levels were determined using ELISA method. Levels of serum albumin, total bilirubin, alanine aminotransferase (ALT) and aspartate transaminase (AST) as well as HCC related biomarkers of alpha fetoprotein (AFP), des-γ-carboxy prothrombin (DCP), gamma glutamyltransferase (GCT-II), α-L-Fucosidase (AFU), CEA and CA19-9 were measured using an automatic biochemical analyzer. Patients’ demographic and clinical characteristics were collected and analyzed.ResultsThe expression of YKL-40 was the highest in HCC patients with HBV-related cirrhosis and the lowest in the healthy control, and the difference was significant compared with other groups. And HCC patients showed markedly higher YKL-40 levels than the HBV-related cirrhosis patients. Patients with higher expression of YKL-40 showed higher ratio of TNM IV, lymphatic metastasis and Child-Pugh C, and higher serum levels of AFP, AFU and CA19-9 than those in the patients with lower levels of YKL-40. YKL-40 level was positively correlated with AFP and AFU. Survival analysis showed patients with higher expression of YKL-40 had shorter 1-year survival time than the patients with lower YKL-40.ConclusionsYKL-40 was elevated in HCC patients with HBV-related cirrhosis and high expression of YKL-40 predicted poor prognosis and shorter 1-year survival.

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Significance of Circulating and Crevicular Matrix Metalloproteinase-9 in Rheumatoid Arthritis-Chronic Periodontitis Association

Journal of Immunology Research ◽

10.1155/2015/218060 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 20

Author(s):

Isabela Silosi ◽

Manole Cojocaru ◽

Lili Foia ◽

Mihail Virgil Boldeanu ◽

Florin Petrescu ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Chronic Periodontitis ◽

Gingival Crevicular Fluid ◽

Serum Levels ◽

Joint Disease ◽

Control Serum ◽

Healthy Control ◽

Crevicular Fluid ◽

And Control ◽

Metalloproteinase 9

In the recent years, statistically significant associations between rheumatoid arthritis (RA) and periodontal disease have been identified. Emerging as a chronic inflammatory joint disease, RA displays various features and pathogenetic events similar to chronic periodontitis (CP). The purpose of this study was to evaluate the utility of determining systemic and crevicular levels of metalloproteinase-9 (MMP-9) as potential biomarkers for association between RA and CP. A total of fifty-six patients were included in the study. The subjects were categorized into four groups as follows: healthy-control (n=21), active RA (n=16), CP (n=14), and RA-CP association (n=12). Assessment of serum and crevicular concentrations of total MMP-9 (active and pro-MMP-9) was based on ELISA technique. The results of this study showed statistically significant differences of serum MMP-9 between patients groups and control. Serum levels of MMP-9 were similar in RA and RA-CP associated patients. Gingival crevicular fluid (GCF) recorded increased MMP-9 levels in RA-CP association subjects as compared to CP. Considering that RA-CP association is characterized by a disregulation of the inflammatory response, MMP-9 may play a role in the pathogenesis of RA-CP association. MMP-9 is therefore a sensitive tool in the diagnosis and management of patients affected by this binomial association.

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Skin tissue expression and serum level of thymic stromal lymphopoietin in patients with psoriasis vulgaris

Dermatology Reports ◽

10.4081/dr.2019.8006 ◽

2019 ◽

Vol 11 (1) ◽

Cited By ~ 3

Author(s):

Oki Suwarsa ◽

Hartati Purbo Dharmadji ◽

Endang Sutedja ◽

Lengga Herlina ◽

Putri Reno Sori ◽

...

Keyword(s):

Psoriasis Vulgaris ◽

Allergic Diseases ◽

Serum Levels ◽

Tissue Expression ◽

Thymic Stromal Lymphopoietin ◽

Enzyme Linked Immunosorbent Assay ◽

Lesional Skin ◽

Healthy Control ◽

Polymerase Chain ◽

The Difference

Thymic stromal lymphopoietin (TSLP) is known to be associated with allergic diseases. It is also suggested that TSLP has a role in autoimmune diseases such as psoriasis; however, the associated pathways remain unknown. There is currently little information on TSLP in psoriasis vulgaris. We investigated TSLP expressions on lesional and non-lesional skin of psoriasis vulgaris patients using reverse transcription- polymerase chain reaction. TSLP level was also investigated in serum from psoriasis vulgaris patients compared to healthy control using enzyme-linked immunosorbent assay. TSLP expression was higher in lesional skin (1.90) compared to non-lesional skin (1.76); however, the difference was not statistically significant (P>0.05). TSLP serum levels were significantly higher in psoriasis patients (287.40 pg/dL) as compared to controls (114.70 pg/dL) (P<0.05). This study concluded that TSLP levels in the serum of psoriasis vulgaris patients are higher than controls. TSLP was also found in keratinocyte of psoriasis patients, the expression was higher in the lesional compared to non-lesional skin; however, this difference is statistically insignificant. These findings suggest that TSLP may play a role in the pathogenesis of psoriasis vulgaris, but its exact role remains unclear.

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Interleukin-34 is associated with hepatocellular carcinoma in chronic hepatitis B patients

10.21203/rs.3.rs-68883/v1 ◽

2020 ◽

Author(s):

Kehui Liu ◽

Yezhou Ding ◽

Yun Wang ◽

Qingqing Zhao ◽

Lei Yan ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Hepatitis B ◽

Tumor Size ◽

Hbv Dna ◽

The Other ◽

Tumor Differentiation ◽

Tumor Number ◽

Control Serum ◽

B Virus ◽

Healthy Control

Abstract Backgrounds: IL‑34 is involved in a number of auto-immunities and cancers. We explored the relationship between serum/hepatic IL‑34 and hepatitis B virus (HBV) related hepatocellular carcinoma (HBV‑HCC) patients.Methods: Serum was obtained from the HBV patients or healthy control with written consents. Liver tissue was obtained from liver biopsy in CHB, HBV related cirrhosis patients or curative resection in HBV‑HCC patients. Serum IL‑34 and MCSF were measured, using ELISA. HepaticIL‑34, MCSF and CD68 were determined, using immunohistochemistry.Results: Serum IL‑34 was 1.7, 1.6 or 1.7 fold higher in HBV‑HCC than that of the other three groups (CHB, HBV related cirrhosis, and healthy control). Serum IL‑34 was significantly reduced after trans‑hepatic arterial chemoembolization (TACE) in HBV‑HCC patients. There was significant correlation between the incidence of HBV‑HCC and IL‑34 (rs=0.160, p<0.05) as well as AFP (rs=0.442, p<0.01). Furthermore, intra-hepatic IL‑34 was higher in HBV‑HCC than that of the other three groups. Intra-hepatic IL‑34 was associated with high HBV‑DNA, HBeAg-, low tumor differentiation and small tumor size of HBV‑HCC patients. Intra-hepaticCD68+ TAMs were increased 1.6 or 1.3 fold in HBV‑HCC compared to that from CHB or HBV-cirrhosis. Intra-hepatic CD68+ TAMs were associated with high HBV‑DNA, high tumor differentiation, big tumor size, abnormal AFP and more tumor number.Conclusions: IL‑34, correlated with HBV‑HCC and IL‑34, may be used as a therapeutic target in precise medicine for management of HBV‑HCC.

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Serum C-reactive protein, fibrinogen and D-dimer in patients with progressive cerebral infarction

Translational Neuroscience ◽

10.1515/tnsci-2016-0013 ◽

2016 ◽

Vol 7 (1) ◽

Cited By ~ 7

Author(s):

Ruo-shi Zang ◽

Hong Zhang ◽

Yan Xu ◽

Sheng-ming Zhang ◽

Xi Liu ◽

...

Keyword(s):

Cerebral Infarction ◽

Functional Disability ◽

C Reactive Protein ◽

Reactive Protein ◽

Progressive Group ◽

Biochemical Analyzer ◽

Automatic Biochemical Analyzer ◽

The Difference ◽

Serum Crp ◽

D Dimer

AbstractObjectiveProgressive cerebral infarctions increase mortality and functional disability through mechanisms which have yet to be completely understood. The goal of this study was to explore the dynamic changes of serum C-reactive protein (CRP), fibrinogen (FIB) and D-dimer (D-D) in order to better characterize progressive cerebral infarction.MethodsThe amount of serum CRP, FIB and D-D was measured in 82 patients with progressive cerebral infarction by taking samples from the internal carotid artery (progressive group), and in 186 patients with non-progressive cerebral infarction (non-progressive group) by using an automatic biochemical analyzer during the next day (day 1), day 3, day 7, and day 14 after being admitted to hospital. Carotid vascular ultrasound and neurological deficit score (National Institutes of Health Stroke Scale, NIHSS) were also recorded.ResultsCarotid stenosis ratio was significantly higher in the progressive group than in the non-progressive group (P < 0.01) on admission. In the progressive group, CRP increased significantly on day 3, followed by a decline on day 7 and day 14, but was significantly higher than those in the non-progressive group (P < 0.01). The levels of FIB and D-D increased in the progressive group more than those in the non-progressive group on day 3, day 7, and day 14 (P < 0.01). The progressive group patients’ NIHSS score gradually increased after admission, which was opposite to the non-progressive group patients whom followed a downward trend. The difference between these two groups was significant (P < 0.01).ConclusionObserving changes of CRP, FIB and D-D may contribute to early identification and timely treatment of progressing ischemic strokes.

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Hepatitis A Seroprevalence Among Patients with Chronic Hepatitis B Infection: A Cross-Sectional Study in Iran During 2016-2017

Infectious Disorders - Drug Targets ◽

10.2174/1871526519666191009144848 ◽

2020 ◽

Vol 20 (5) ◽

pp. 748-751

Author(s):

Seyed Ali Dehghan Manshadi ◽

Neda Alijani ◽

Mohammadreza Salehi ◽

Omid Dadras ◽

SeyedAhmad SeyedAlinaghi ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis B ◽

Chronic Hepatitis B ◽

Hepatitis A ◽

Cross Sectional Study ◽

University Hospital ◽

Cross Sectional ◽

Reasonable Approach ◽

The Difference ◽

Prevention Method

Introduction: The aim of this study was to determine the prevalence of exposure to hepatitis A by means of serologic markers in chronic hepatitis B patients, with the secondary aim of finding the best prevention method for hepatitis A infection in susceptible groups of our setting. Methods: During the period between 2016 and 2017, we recruited 403 hepatitis B patients aged more than 14 years and regularly attending the infectious diseases clinic at a referral university hospital, Tehran, Iran. A blood sample was collected from all the patients and tested for hepatitis A IgG. The data was analyzed by SPSS v.19. Results: Although none of the patients had previously received hepatitis A vaccine, the results for serologic level of hepatitis A IgG, demonstrated positive results in 379 (94%) cases. The mean age of patients with negative and positive IgG was 29.17 and 42.46 years, respectively; the difference was statistically significant (P≤0.001). The majority of seronegative patients were young adults aged < 25 years and 25 to 35 years (P <0.001). Conclusion: Seroprevalence of hepatitis A in chronic HBV patients in Iran is high. As HBV infected patients younger than 35 years could be seronagative for HAV infection, evaluation of these patients for HAV infection and vaccination of seronegative patients would be a reasonable approach.

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Efficacy and Safety of a 3-Antigen (Pre-S1/Pre-S2/S) Hepatitis B Vaccine: Results of a Phase 3 Randomized Clinical Trial in the Russian Federation

Clinical Infectious Diseases ◽

10.1093/cid/ciaa1649 ◽

2020 ◽

Author(s):

Elena V Esaulenko ◽

Aleksey A Yakovlev ◽

Genady A Volkov ◽

Anastasia A Sukhoruk ◽

Kirill G Surkov ◽

...

Keyword(s):

Hepatitis B ◽

Geometric Mean ◽

Serious Adverse Events ◽

Double Blind ◽

Antigen Vaccine ◽

Single Antigen ◽

Antibody Titers ◽

The Russian Federation ◽

The Difference ◽

Mean Concentration

Abstract Background This study compares the immunogenicity and safety of a 3-antigen (S/pre-S1/pre-S2) hepatitis B (HepB) vaccine (3AV), to a single antigen vaccine (1AV) in adults to support the registration of 3AV in Russia. Methods We conducted a randomized, double-blind, comparative study of 3-dose regimens of 3AV (10 μg) and 1AV (20 µg) in adults aged 18–45 years. We evaluated immunogenicity based on hepatitis B surface (HBs) antibody titers at days 1, 28, 90, 180, and 210, adverse and serious adverse events (SAEs) to study day 210. The primary outcome was based on the difference in rates of seroconversion at day 210 (lower bound 95% confidence interval [CI]: > − 4%). Secondary outcomes were seroprotection rates (SPR), defined as anti-HBs ≥10 mIU/mL and anti-HBs geometric mean concentration (GMC). Results Rate of seroconversion in 3AV (100%) was noninferior to 1AV (97.9%) at study day 210 (difference: 2.1%, 95% CI: −2.0, 6.3%]) but significantly higher at study day 28. SPR at study day 210 was >97% in both arms. Anti-HBs titers were significantly higher at study days 90 (P = .001) and 180 (P = .0001) with 3AV. Sex, age, and body mass index (BMI) had no impact on anti-HBs titers. The rates of local reactions related to vaccination were similar between vaccine arms (3AV vs 1AV) after the first (30% vs 18.8%, P = .15), second (20.0% vs 14.6%, P = .33), and third vaccination (14.9% vs 23.4%, P = .22). No SAEs were reported. Conclusions 3AV was noninferior to 1AV. 3AV induced high SPR, and there were no safety concerns. Clinical Trials Registration. NCT04209400.

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Low serum vitamin B12 levels are associated with degenerative rotator cuff tear

BMC Musculoskeletal Disorders ◽

10.1186/s12891-021-04231-7 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Jae Hwa Kim ◽

Go-Tak Kim ◽

Siyeoung Yoon ◽

Hyun Il Lee ◽

Kyung Rae Ko ◽

...

Keyword(s):

Logistic Regression ◽

Rotator Cuff ◽

Univariate Analysis ◽

Serum Vitamin ◽

Serum Levels ◽

Control Group ◽

Mineral Density ◽

Calcium Phosphorus ◽

Healthy Control ◽

Low Serum

Abstract Background Vitamin B12 (Vit B12) deficiency results in elevated homocysteine levels and interference with collagen cross-linking, which may affect tendon integrity. The purpose of this study was to investigate whether serum Vit B12 levels were correlated with degenerative rotator cuff (RC) tear. Methods Eighty-seven consecutive patients with or without degenerative RC tear were enrolled as study participants. Possible risk factors (age, sex, medical history, bone mineral density, and serum chemistries including glucose, magnesium, calcium, phosphorus, zinc, homocysteine, Vitamin D, Vit B12, homocysteine, and folate) were assessed. Significant variables were selected based on the results of univariate analyses, and a logistic regression model (backward elimination) was constructed to predict the presence of degenerative RC tear. Results In the univariate analysis, the group of patients with degenerative RC tear had a mean concentration of 528.4 pg/mL Vit B12, which was significantly lower than the healthy control group (627.1 pg/mL). Logistic regression analysis using Vit B12 as an independent variable revealed that Vit B12 concentrations were significantly correlated with degenerative RC tear (p = 0.044). However, Vit B12 levels were not associated with tear size. Conclusion Low serum levels of Vit B12 were independently related to degenerative RC tear. Further investigations are warranted to determine if Vit B12 supplementation can decrease the risk of this condition.

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Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma

Cancers ◽

10.3390/cancers13030529 ◽

2021 ◽

Vol 13 (3) ◽

pp. 529

Author(s):

Silvia Selene Moreno-Guerrero ◽

Arturo Ramírez-Pacheco ◽

Luz María Rocha-Ramírez ◽

Gabriela Hernández-Pliego ◽

Pilar Eguía-Aguilar ◽

...

Keyword(s):

Overall Survival ◽

Genetic Polymorphisms ◽

Poor Prognosis ◽

Serum Levels ◽

Control Group ◽

Mexican Children ◽

Genotype Frequencies ◽

Pcr Rflp ◽

The Impact ◽

Circulating Levels

There is evidence that high circulating levels of IL-6 and IL-8 are markers of a poor prognosis in various types of cancer, including NB. The participation of these cytokines in the tumor microenvironment has been described to promote progression and metastasis. Our objective was to evaluate the prognostic role of genetic polymorphisms and serum levels of IL-6 and IL-8 in a cohort of Mexican pediatric patients with NB. The detection of the SNPs rs1800795 IL-6 and rs4073 and rs2227306 IL-8 was carried out by PCR-RFLP and the levels of cytokines were determined by the ELISA method. We found elevated circulating levels of IL-8 and IL-6 in NB patients compared to the control group. The genotype frequencies of the rs1800795 IL-6 and rs4073 IL-8 variants were different between the patients with NB and the control group. Likewise, the survival analysis showed that the GG genotypes of rs1800795 IL-6 (p = 0.014) and AA genotypes of rs4073 IL-8 (p = 0.002), as well as high levels of IL-6 (p = 0.009) and IL-8 (p = 0.046), were associated with lower overall survival. We confirmed the impact on an adverse prognosis in a multivariate model. This study suggests that the SNPs rs1800795 IL-6 and rs4073 IL-8 and their serum levels could be promising biomarkers of a poor prognosis, associated with overall survival, metastasis, and a high risk in Mexican children with NB.

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PSVII-17 Program Chair Poster Pick: Reproductive function of cows depending on lipid metabolism

Journal of Animal Science ◽

10.1093/jas/skaa278.529 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 293-294

Author(s):

Murat Baymishev ◽

Sergey Eremin ◽

Kirill Plemyashov ◽

Khamidulla Baymishev ◽

Igor Konopel’tsev ◽

...

Keyword(s):

Lipid Peroxidation ◽

Live Weight ◽

Reproductive Function ◽

Serum Levels ◽

Total Serum ◽

Dry Period ◽

Lipid Peroxidation Products ◽

Synthetic Function ◽

The Difference ◽

Oxidized Products

Abstract The aim of the study was to determine the etiopathogenesis of reproductive dysfunction in highly productive cows. Studies were conducted on cows of black-motley breed, aged 3–5 lactations, with a live weight of 480–520 kg. Cows were divided into two groups. The first group included 20 cows effective inseminated after the first insemination. The second group included 17 cows that did not fertilize after the first insemination. Blood samples (n = 253) were taken from 4–7; 14–17; 24–34; 35–50; 88–95; 195–210 days of pregnancy; during the beginning of dry period (DP); 1–4 days before calving and on the first day after calving. The content of total serum lipids in pregnant animals was 15.8% higher compared to unfertilized animals. In non-pregnant cows, a decrease in free cholesterol (by 28.65%), especially in its ether-bound fraction (14.91%), indicates a lower synthetic function of the liver, which is confirmed by the low serum levels of vitamins A and E (a difference of 11.3 and 23.65%). The DP was characterized by a significant increase in the products of lipid peroxidation - malondialdehyde by 12–17%, diene and triene conjugates - by 10–15 and 8–15%, respectively, and 1–4 days before calving in cows, which subsequently had a pathological calving, in particular - retention of placenta (5 cows), were characterized by an increased content in the blood plasma of lipid peroxidation products. Thus, the difference in the number of dienes, trienes, and malondialdehyde products was 14.0, 12.0, and 23.5%, compared with animals without obstetric pathology. On the first day after calving, this difference increased even more. Cows with retention of placenta had a significantly higher level of lipid peroxidation products and were characterized by a predominance of oxidized products over the antiradical thiol disulfide and ascorbate systems restored in the antioxidant balance.

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Serum otolin-1 as a biomarker for benign paroxysmal positional vertigo: a case-control study

The Journal of Laryngology & Otology ◽

10.1017/s0022215121001274 ◽

2021 ◽

pp. 1-4

Author(s):

D V K Irugu ◽

A Singh ◽

H Yadav ◽

H Verma ◽

R Kumar ◽

...

Keyword(s):

Serum Level ◽

Benign Paroxysmal Positional Vertigo ◽

Case Control Study ◽

Serum Levels ◽

Case Control ◽

Control Group ◽

Positional Vertigo ◽

The Difference ◽

Control Study ◽

Paroxysmal Positional Vertigo

Abstract Objectives This study aimed to evaluate serum otolin-1 levels in patients with benign paroxysmal positional vertigo and to compare these levels with healthy individuals. Method This was a case-control study. After obtaining institutional ethical committee clearance, the serum level of otolin-1 was calculated in adult individuals (18–75 years old) who were divided into group 1 (patients presenting with benign paroxysmal positional vertigo) and group 2 (healthy patients without benign paroxysmal positional vertigo as the control group). Data analysis was carried out to compare the serum levels in the cases and controls. A p-value less than 0.05 was considered significant. Results A total of 70 age-matched individuals (cases, n = 40; controls, n = 30) were included in the study. The mean serum level of otolin-1 was 636.8 pg/ml (range, 259–981 pg/ml) in the group of patients with benign paroxysmal positional vertigo and 236.2 pg/ml (range, 189–370 pg/ml) in the control group. The difference was statistically significant (p = 0.0000). Conclusion The serum levels of otolin-1 in patients with benign paroxysmal positional vertigo are significantly higher compared with individuals without benign paroxysmal positional vertigo.

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