BIOPROSPECTING OF MARINE HALOPHYTE SALICORNIA EUROPAEA L. AND EVALUATION OF ITS BIOLOGICAL POTENTIAL WITH SPECIAL REFERENCE TO ANTICANCER ACTIVITY

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (05) ◽  
pp. 47-56
Author(s):  
P Samuel ◽  
◽  
J. Vijaya Kumar ◽  
T Selvarathinam ◽  
R. Deena dhayalan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Soxhlet Extraction ◽  
Salicornia Europaea ◽  
Chemical Screening ◽  
Biological Potential ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Mcf7 Cell Line ◽  
The Individual ◽  
Promising Source

The study was caried out with the intention to bring out the biological prospective nature of the marine halophyte Salicornia europaea L. The bioactive phytochemicals of the halophyte were extracted. The chemicals in the crude extract were evaluated for cytotoxic activity against MCF7 cell line by MTT assay. The marine halophyte a was collected, washed and chopped into 5cm long parts and shade dried for 20 – 25 days in a dark room. The dehydrated and bleached plant material was subjected to Soxhlet extraction. Two solvents, viz methanol and ethyl acetate, were used to prepare decoction. The extracts were dried using rotary vacuum evaporator. chemical screening by GC-MS unveiled the presence of 32 compounds in ethyl acetate and 29 in methanol. The 3D structures of the phytochemicals were retrieved from Pub Chem. Cytotoxic study showed promising results. The IC50 values of the individual extracts were evaluated and ethyl acetate extract exhibit minimum IC50 value and it was estimated to be 97.9μg/mL wereas methanol extract exhibit a minimum IC50 value of 117.1μg/mL. Hence, the study concludes that S. europaea L. provides a promising source of lead compounds that could be exploited in near future to treat cancer.

scholarly journals Identification and Heme Polymerization Inhibition Activity (HPIA) Assay of Ethanolic Extract and Fraction of Temu Mangga (Curcuma mangga Val.) Rhizome

2020 ◽  
Vol 20 (1) ◽  
pp. 64-72
Author(s):  
Dhina Fitriastuti ◽  
Tatang Shabur Julianto ◽  
Annisa Wahyu Nur Iman
Keyword(s):  
Ethyl Acetate ◽  
Antimalarial Activity ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Inhibition Activity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Vacuum Liquid Chromatography ◽  
Active Substances ◽  
Better Than

Curcuma mangga Val. is one of Indonesian herbs from Zingiberaceae family that is under explored and could contain potentially active substances to serve as an antimalarial. This research intends not only to examine the antimalarial activity by means of heme polymerization inhibitor mechanism by using the ethanolic extract and fraction of Curcuma mangga Val. but also to identify its compound classification. The extract of temu manga was obtained by Soxhlet extraction method using ethanol solvent followed by fractionation using Vacuum Liquid Chromatography with solvent sequence n-hexane, n-hexane: ethyl acetate (2:1), ethyl acetate and ethanol. The extract and fraction were analyzed by using LC-MS and GC-MS. Activities of hem polymerization inhibition showed by IC50 values which were obtained from analysis of relationship between concentration sample and the percentage of inhibition using the PROBIT on statistical software. The result of HPIA assay shows that the IC50 value of ethanolic extract and ethanolic fraction of Curcuma mangga Val. rhizome are 2.273 and 1.479 mg/mL, respectively. It clearly shows that the heme polymerization inhibition activity of ethanolic fraction relatively better than that of ethanolic extract. Phytochemical screening determines the ethanolic extract contains saponin, terpenoid, and phenol while the ethanolic fraction contains terpenoid. Thus, terpenoid compound is presumed to be the inhibitor of heme polymerization. The results of analysis with LC-MS and GC-MS showed that the active compounds suspected to inhibit heme polymerization in ethanolic extract and fraction were (E) -labda-8 (17), 12-dien-15,16-dial and di-n-octyl phthalate, respectively. Keywords: antimalarial, Curcuma mangga Val., heme polymerization

GLORIOSA SUPERBA L. – AN ENDANGERED PLANT OF TAMILNADU, YET TO BE EXPLORED AND NEED TO BE CONSERVED

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (07) ◽  
pp. 60-62
Author(s):  
P. Rajeswari ◽  
◽  
P Samuel ◽  
D. N. P Sudarmani ◽  
K Amirtharaj ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Soxhlet Extraction ◽  
Ethyl Acetate Fraction ◽  
Extensive Literature ◽  
Gloriosa Superba ◽  
Methanol Fraction ◽  
Anticancer Properties ◽  
Ic50 Value ◽  
Extraction Unit ◽  
Mcf7 Cell Line

Gloriosa superba L. is commonly being called as glory lily. This perennial climber is designated as the State flower of Tamilnadu. It is extensively cultivated for colcichine, an alkaloid obtained from the seed being exploited for its medicinal properties. The present investigation on this climber was started with an intention to bring out the biomedical potentials with special reference to its anticancer properties. The plant was extracted with methanol and ethyl acetate using Soxhlet extraction unit and the extract was concentrated using vacuum rotavapor. The presence of 15 different compounds in methanol fraction and 1 compound in ethyl acetate fraction were determined by GC-MS. Extensive literature survey suggested that few records was available on anticancer properties of Gloriosa superba L. Hence, an attempt was made to evaluate its anticancer potentials The IC50 value of the compound against MCF7 cell line was found to be 84.23μg/mL.

scholarly journals Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC. leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. G. Toledo ◽  
J. G. de L. de Souza ◽  
C. B. Santana ◽  
A. P. Mallmann ◽  
C. V. dos Santos ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Biological Potential ◽  
Sequestration Potential ◽  
Ic50 Value ◽  
Organic Extracts ◽  
Phytochemical Profile ◽  
Promising Source

Abstract The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 34-38
Author(s):  
Siti Warnasih ◽  
Diana Widiastuti ◽  
Uswatun Hasanah ◽  
Laksmi Ambarsari ◽  
Purwantiningsih Sugita
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Palm Fruit ◽  
Ic50 Value ◽  
Dpph Method ◽  
Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

scholarly journals CYTOTOXIC EFFECT FROM ETHYL ACETATE-METHANOL SUBFRACTION OF CARRISA CARANDAS L TOWARD HELA CELLS BY IN VITRO TEST

2017 ◽  
Vol 10 (14) ◽  
pp. 21
Author(s):  
Mamik P. Rahayu ◽  
Reslely Harjanti ◽  
Mae S. H. Wahyuningsih ◽  
Supargiyono .
Keyword(s):  
Ethyl Acetate ◽  
Colorless Crystal ◽  
Cytotoxic Effect ◽  
Chloroform Fraction ◽  
In Vitro Test ◽  
Preventive Effect ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Vitro Test

Objective: Cervical cancer is a malignant type of cancer, often affects women, particularly in developing countries. Carrisa carandas leaves contained many secondary metabolites that had potency as an anticancer. The purpose of this study was to understand the cytotoxic effect of subfraction of Carrisa carandas leaves against HeLa cells.Methods: Chloroform fraction was separated by VLC gradually with n-hexane–chloroform–ethyl acetate and methanol. The same profiles from eluent chloroform–ethyl acetate composed fraction 18-26 were categorized as Fr4 and ethyl acetate-methanol composed fraction 27-30 as Fr5. The cytotoxic effect was evaluated by MTT assay on HeLa cellsResults: The result showed that the cytotoxic effect of subfraction Fr4 and Fr5 had IC50 values of 177 mg/ml and 98 mg/ml, respectively. Colorless crystal of Subfraction Fr 5-3 had IC50 value of 333 mg/ml. Subfraction Fr 5 showed effective cytotoxic activity than the others. Conclusion: It had chemo-preventive effect against cancer cellsConclusion: This study applied MTT (Microculture Tetrazolium) method by in vitro test. The advantages of this method are relatively rapid, sensitive and accurate

scholarly journals Olive Mill Wastewater Polyphenol-Enriched Fractions by Integrated Membrane Process: A Promising Source of Antioxidant, Hypolipidemic and Hypoglycaemic Compounds

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 602 ◽  
Author(s):  
Rosa Tundis ◽  
Carmela Conidi ◽  
Monica R. Loizzo ◽  
Vincenzo Sicari ◽  
Alfredo Cassano
Keyword(s):  
High Performance ◽  
Olive Mill Wastewater ◽  
In Vitro Tests ◽  
Ic50 Value ◽  
Bleaching Test ◽  
Ic50 Values ◽  
Olive Waste ◽  
Promising Source ◽  
Olive Mill

The valorisation of food wastes is a challenging opportunity for the green, sustainable, and competitive development of industry. The recovery of phenols contributes to the sustainability of olive waste sector, reducing its environmental impact and promoting the development of innovative formulations of interest for pharmaceutical, nutraceutical, and cosmeceutical applications. In this work, olive mill wastewater was treated through a combination of microfiltration (MF), nanofiltration (NF), and reverse osmosis (RO) in a sequential design to produce polyphenol-enriched fractions that have been investigated for their chemical profile using ultra-high-performance liquid chromatography (UHPLC), and their potential antioxidant, hypolipidemic, and hypoglycaemic activities. RO retentate exhibited the highest content of hydroxytyrosol, tyrosol, oleuropein, verbascoside, vanillic acid, and luteolin. In particular, a content of hydroxytyrosol of 1522.2 mg/L, about five times higher than the MF feed, was found. RO retentate was the most active extract in all in vitro tests. Interestingly, this fraction showed a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) radicals scavenging activity with an IC50 value of 6.9 μg/mL and a potential inhibition of lipid peroxidation evaluated by the β-carotene bleaching test with IC50 values of 25.1 μg/mL after 30 min of incubation. Moreover, RO retentate inhibited α-amylase and α-glucosidase with IC50 values of 65.3 and 66.2 μg/mL, respectively.

scholarly journals Antioxidant and antimicrobial activities of Penicillium sp. lectins

2019 ◽  
Vol 71 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Ram Singh ◽  
Amandeep Walia
Keyword(s):  
Biomedical Applications ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Peroxide Radical ◽  
Gram Positive Bacteria ◽  
Penicillium Sp ◽  
Biological Potential ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Antioxidant And Antimicrobial Activities

Lectins are a diverse group of proteins of non-immune origin that interact specifically with glycans. Owing to their specificity, they can mediate various cellular and molecular recognition processes. To explore information on biological activities of lectins from Penicillium duclauxii, P. proteolyticum and P. griseoroseum, they were investigated for their antioxidant and antimicrobial activities. Penicillium sp. lectins exhibited moderate antioxidant activity. P. duclauxii, P. proteolyticum and P. griseoroseum lectins inhibited DPPH with an IC50 value of 71.42, 75.04 and 82.11 ?g/mL, respectively. P. duclauxii, P. proteolyticum and P. griseoroseum lectins inhibited the hydrogen peroxide radical with IC50 values of 198.57, 209.76 and 215.31 ?g/mL, respectively. P. duclauxii and P. proteolyticum lectins exhibited potent antibacterial activity against Gram-negative bacteria. P. griseoroseum lectin inhibited only Gram-positive bacteria. Penicillium sp. lectins did not exhibit antifungal activity. The biological potential of Penicillium sp. lectins will help to understand their biomedical applications. This is the first report on the antioxidant and antimicrobial activities of purified lectins from Penicillium sp.

scholarly journals Antioxidant Potential of Madura Knife Scallop (Solen sp) Extract as a Prevention of Oxidative Stress

2020 ◽  
Vol 15 (3) ◽  
pp. 382-389
Author(s):  
Mirwa Adiprahara Anggarani ◽  
Roy Januardi Irawan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Cell Structure ◽  
Oxygen Species ◽  
Meat Extract ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Reactive Molecule

Reactive Oxygen Species (ROS) are produced by humans as a result of cellular metabolism and environmental factors such as pollutants or cigarette smoke. ROS is a very reactive molecule and has the ability to damage cell structure. Oxidative stress is a condition between oxidants and antioxidants that is not balance, pathophysiologically, oxidative stress can trigger the risk of various diseases including hypertension, atherosclerosis, diabetes, heart failure, stroke and other chronic diseases. Oxidative stress can be overcome by intake of antioxidants. The purpose of this study was to determine the antioxidant activity contained in the meat and shells of a knife scallop (Solen sp.) that lives in Bangkalan waters, Madura. The method used in this study is an analysis of antioxidant activity with the DPPH method. Knife scallop has antioxidant activity which is evident from the IC50 values obtained. IC50 values of the shell and meat extract with ethanol solvent were 489.56 ppm and 748.49 ppm. IC50 values of the shell and meat extract with ethyl acetate solvent were 916.43 ppm and 2045.93 ppm. While the IC50 value of the shell and meat extract with chloroform solvent was 119.37 ppm and 1692.80 ppm. Based on IC50 data of knife scallop shell and meat extracts on the 3 types of solvents, it can be concluded that knife scallop’s shell and meat extract with ethanol solvent has the greatest antioxidant activity compared to ethyl acetate and chloroform solvents.

scholarly journals Potensi Kulit Batang Bungur (Lagerstroemia loudonii Teijsm and Binn.) Sebagai Herbal Antidiabetes dengan Mekanisme Penghambat Alfa-glukosidase

2018 ◽  
Vol 1 (3) ◽  
pp. 117-120
Author(s):  
Soraya Riyanti ◽  
Julia Ratnawati ◽  
Muhammad Ibnu Shaleh ◽  
Asep Gana Suganda
Keyword(s):  
Ethyl Acetate ◽  
Colorimetric Method ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Water Fraction ◽  
Ic50 Value ◽  
The Family ◽  
Ic50 Values ◽  
Alpha Glucosidase

Tumbuhan bungur (Lagerstroemia loudonii T.B.) termasuk dalam famili Lytrharceae. Famili Lythraceae telah diketahui memiliki aktivitas farmakologi sebagai antidiabetes, antiinflamasi, antimikroba, serta antiobesitas. Daun dan buah bungur (Lagerstroemia loudonii T.B.) memiliki aktivitas dalam menghambat alfa-glukosidase. Berdasarkan teori khemotaksonomi didalam tumbuhan, kemungkinan bagian lain dari tumbuhan bungur memiliki aktivitas dan kandungan kimia yang sama, sehingga dilakukan pengujian aktivitas penghambatan terhadap alfa-glukosidase pada bagian kulit batang bungur. Ekstraksi simplisia dilakukan dengan cara maserasi selama 24 jam menggunakan pelarut etanol 96%. Proses fraksinasi menggunakan cara Ekstraksi Cair-Cair (ECC) dengan pelarut n-heksana, etil asetat dan air. Pengujian aktivitas penghambatan alfa-glukosidase secara in vitro menggunakan metode kolorimetri dengan alat spektrofotometer UV-VIS pada panjang gelombang 400,4 nm dengan substrat p-nitrofenil-α-D-glukopiranosid (PNPG). Akarbose digunakan sebagai pembanding. Hasil penelitian menunjukkan bahwa ekstrak, fraksi air, fraksi etil asetat dan fraksi n-heksana memiliki nilai IC50 berturut-turut sebesar 240,53±0,47 μg/ml, 186,111±1,02 μg/ml, 79,479±0,52 μg/ml dan 113,101±0,46 μg/ml. Nilai IC50 akarbose adalah sebesar 10,457±1,48 μg/ml. Ekstrak dan fraksi-fraksi (air,etil asetat dan n-heksana) kulit batang bungur mampu menghambat aktivitas enzim α-glukosidase. Aktivitas yang paling baik ditunjukan oleh fraksi etil asetat dengan nilai IC50 sebesar 79,479±0,52 μg/ml.   Bungur (Lagerstroemia loudonii T.B.) is included in the Family  Lytrharceae. The Lythraceae has been known to have pharmacological activity as antidiabetic, anti-inflammatory, antimicrobial, and antiobesity. Leaves and fruits of bungur (Lagerstroemia loudonii T.B.) have activities to inhibit alpha-glucosidase. Based on the chemotaxonomy theory in plants, it is possible that other parts of the bungur plant have the same chemical activity and content, Hence the study to evalute the  inhibitory activity against alpha-glucosidase was carried out on its bark stem.The extraction of dried powder material was carried out by maceration for 24 hours using 96% ethanol. The extract was fractionated by Liquid-Liquid Extraction (ECC) method with n-hexane, ethyl acetate and water. The In vitro study of alpha-glucosidase inhibition activity using a colorimetric method with a UV-VIS spectrophotometer at a wavelength of 400.4 nm with a p-nitrophenyl-α-D-glucopiranoside (PNPG) substrate was performed. Akarbose was used as a standard drug.The results showed that extract, water fraction, ethyl acetate fraction and n-hexane fraction showed IC50 values ​​of 240.53 ± 0.47 μg / ml, 186.111 ± 1.02 μg / ml, 79.497 ± 0.52 μg/ ml and 113.101 ± 0.46 μg / ml, respectively. The IC50value of bungur was 10.457 ± 1.48 μg / ml. Extracts and fractions (water, ethyl acetate and n-hexane) of bark stem were able to inhibit the activity of α-glucosidase. Theethyl acetate fraction showed the strongestactivity with IC50 value of 79,479 ± 0.52 μg / ml

scholarly journals STUDI IN VITRO POTENSI ANTIOKSIDAN DAN AKTIFITAS ANTIDIABETES FRAKSI ETIL ASETAT BUAH PARIJOTO (Medinilla speciosa B.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Rissa Vifta ◽  
Wilantika Wilantika ◽  
Yustisia Dian Advistasari
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Ethyl Acetate Fraction ◽  
In Vitro Assay ◽  
Antidiabetic Activity ◽  
Vitro Assay ◽  
Antioxidants Activity ◽  
Ic50 Value ◽  
Ic50 Values

ABSTRACT Parijoto fruit (Medinilla speciosa B) contains the flavonoid which is one of the phenolic groups compounnd. Flavonoids has biological activities as anti free radical and antionxidants. The aim of this research was to evaluate the potency of ethyl acetat fraction of M.speciosa B. Extract as an antioxidants and antidiabetic. Evaluation of antioxidants activity was carried out by in vitro assay using the ABTS method (2.2 azinobis (3-ethylbenzotiazolin) -6-sulfonic acid), while the antidiabetic assay was carried out using the Nelson-Somogyi method. Research begins with the process of determination, extraction, fravtionation and contiunued by examination of each variable. The parameters of antioxidants activity was determined by IC50 values, while antidiabetic activity was measured by percentage of decreasing of glucoce levels. The results of antioxidants activity showed that ethyl acetate fraction of M. Speciosa B. had antioxidants activity with an IC50 value of 4,246 ppm with a very strong category. In line with these results, ethyl acetate fraction of M. speciosa B. had reduced glucoce levels with an optimal decrease of 50.21% a concentration of 40 ppm.   ABSTRAK Buah Parijoto (Medinilla speciosa B.) mengandung senyawa aktif flavonoid yang merupakan salah satu golongan fenolik. Flavonoid memiliki aktifitas biologis sebagai antiradikal bebas dan antioksidan. Penelitian dilakukan dengan tujuan mengetahui kemampuan fraksi etil asetat M. speciosa B sebagai antioksidan dan antidiabetes. Pengujian aktifitas antioksidan dilakukan secara in vitro dengan metode ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat), sedangkan uji antidiabetes dilakukan menggunakan metode Nelson-Somogyi. Penelitian diawali dengan proses determinasi, ekstraksi, fraksinasi, dan dilanjutkan dengan pengujian pada masing-masing variabel. Parameter aktifitas antioksidan diwujudkan dengan nilai IC50, sedangkan aktiftas antidiabetes diukur dengan persen penurunan kadar glukosa. Hasil pengujian aktifitas antioksidan menunjukkan bahwa fraksi etil asetat memiliki aktifitas antioksidan dengan nilai IC50 sebesar 4.14±0.08 ppm dengan kategori sangat kuat. Sejalan dengan hasil tersebut, fraksi etil asetat Buah Parijoto (M. speciosa B.) memilili kemampuan dalam menurunkan kadar glukosa dengan penurunan secara optimal sebesar 50.21±0.47% pada konsentrasi 40 ppm.    

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