Inhibition of some aquatic and aromatic plants extracts on fungal infection on common carp (Cyprinus carpio L.) and its eggs

Present study was carried out to investigate the inhibition effect of extracts of some aquatic and aromatic plants on three fungi genera (Aspergillus, Penicillium and Saprolegnia) infecting common carp and its eggs. The inhibition test was done for 15 species of plants represented: Hornwort Ceratophyllum demersum L.; Water hyssop Bacopamonnieria (L.) Hayata et Mastum; Eurasian watermilfoil Myriophyllum spicatumL.; curled pondweed Potamogeton crispus L.; Widgeon-grass Ruppia maritimaL.; Mint Mentha spicata L.; Ginger Zingiber officinale Roscoe; Onion Allium cepa L.; Leek Allium ampeloprasum L. var. porrum (L.) ;Damas Conocarpus lancifolius Engl. & Diels; Garden cress Lepidium sativum L.; Brant's oak Quercus brantii Lindl. and Eggplant Solanum melongena L. using the manner of food poisoning in Petri dishes. The results showed a difference in the inhibitory effect of the extracts according to the species of plants and fungi. The highest percentage of inhibition on Aspergillus was by using Q. brantii (79%) and the lowest was the R. maritime (18%). The highest percentage of inhibition on Penicillium was obtained by using the water extract of Z. officinale (81%) and lowest was the S. melongena (2%). M. spicatum extract has the highest inhibitory effect on Saprolegnia (100%) while the lowest was A. sativum (38.8%).

2019 ◽  
Vol 31 (1) ◽  
pp. 35-46
Author(s):  
Seyedmohammadreza Ojaghian ◽  
Meisam Saremi ◽  
Saeid Pashaei

The objective of this study was to evaluate antifungal activity and resistance inducing potential of crude extracts derived from neem (Azadirachta indica) and ginger (Zingiber officinale) against three isolates of Sclerotinia sclerotiorum, the causal agent of mustard white mold under in vitro and in vivo conditions. In addition, enzymatic tests were carried out to assess the effect of crude extracts on activities of resistance-inducing enzymes in mustard leaves. The results showed that ethanol extracts of neem and ginger at concentration 2 g/l were able to reduce mycelial growth of the pathogen (isolate 3) by 61.5 and 44.3%, respectively. The ethanol extracts of neem and ginger at concentration 2 g/l reduced infection radius on plant leaves from 9.7 in control to 3.1 and 3.4, respectively, due to antifungal efficacy. In addition, ethanol extracts of neem and ginger at concentration 2 g/l decreased infection radius (isolate 1) on plant leaves from 9.5 in control to 2.1 and 2.3, respectively, seven days after application. Enzymatic analyses showed significant increase in level of chitinases, β-1,3-glucanase, Phenylalanine ammonia lyase and Peroxidase due to application of ethanol extracts of neem and ginger.


2017 ◽  
Vol 9 (13) ◽  
pp. 112 ◽  
Author(s):  
Zamirah Zainal-Abidin ◽  
Nor Akmal Abdul-Wahab ◽  
Muhamad Kamil Ghazi-Ahmad ◽  
Shahida Mohd-Said

This study evaluates the antibacterial effects of Zingiber officinale essential oil and Orthosiphon stamineus water extract against Enterococcus faecalis. The herbs were prepared in various concentrations to determine their minimum inhibitory concentrations (MIC) and growth inhibitory effect. Anti-adhesion activities of the herbs were determined by co-incubation with E. faecalis cultures for 6 and 24 h. Biofilm disruption activities were determined by adding the studied herbs into preformed E. faecalis biofilm. The effects on the morphology of E. faecalis grown as biofilm were studied using scanning electron microscopy (SEM). The MICs of ginger oil and O. stamineus extract were 0.31 and 25 mg/mL, respectively. Between the tested herbs, ginger exhibited greater inhibitory effects on the growth of E. faecalis grown in suspension mode. Both herbs generally showed anti-adhesion activities in inverse concentration-dependent manner. No significant biofilm disruption activities by both herbs were observed. SEM analyses showed E. faecalis cell surface changes in the treated biofilm. The studied herbs may have compromised the integrity of the bacterial cell membrane. These findings suggest that the studied herbs may have better antibacterial activities against E. faecalis in suspension mode compared to biofilm mode, with ginger oil showed greater antibacterial activity compared to O. stamineus extract.


2017 ◽  
pp. 201-207 ◽  
Author(s):  
Dragana Plavsic ◽  
Gordana Dimic ◽  
Djordje Psodorov ◽  
Dragan Psodorov ◽  
Ljubisa Saric ◽  
...  

Aromatic plants are one of the most important sources of biologically active secondary metabolites, which possess various antimicrobial characteristics. The aim of this work was to examine the effect of antifungal activities of mint and caraway essential oils against the selected fungi. Eight species of molds were selected for antifungal testing: Alternaria alternata, Aspegillus flavus, A. niger, A. versicolor, Eurotium herbariorum, Penicillium aurantiogriseum, P. chrysogenum and P. expansum. Testing of essential oils antifungal activity against the selected species was conducted using the disc diffusion method by adding mint and caraway essential oils (0.5, 1, 5, and 10 ?l per disc). Antifungal activity of essential oils was expressed by the diameter of inhibition zone (mm). The most powerful effect of mint essential oil was recorded against E. herbariorum, as its growth was completely inhibited by the quantity of 5 ?l. The weakest inhibitory effect was observed against P. chrysogenum (inhibition zone 13.67 mm) by the quantity of 10 ?l. The most powerful antifungal activity of caraway was observed against E. herbariorum as growth was completely inhibited by the quantity of 10 ?l. The weakest inhibitory effect was observed against A. niger (inhibition zone 28 mm) by the quantity of 10 ?l.


2021 ◽  
Author(s):  
Ashraf fawzy mosa ◽  
Mostafa abo Elhoda Mohamed

Abstract Background: Covid-19 Virus infection poses significant global health challenges and considered a global epidemic sweeping all countries of the world Which prompted scientists around the world to search for a quick or safe treatment to preserve people's lives .So far, options for controlling and treating the disease have not been revealed. The current study was conducted to evaluate the effectiveness of pomegranate peels extract against the Covid-19 virus in the laboratory. Methods: In this research, tow methods of extraction are carried out ethyl alcohol and distal water extract of pomegranate peels . activity of the extract assessed using 50% Tissue Culture Infectious Doses (TCID50) method in Vero E6 cells. Results: Pomegranate peels extract had the highest inhibitory effect against Covid -19 virus with IC50 value of 0.125, 0.0625 and 0.031256 μl in Vero E6 cells. Conclusion: Based on our results, the aqueous extract of pomegranate peels can inhibit Covid-19 virus replication in vitro.


2019 ◽  
Vol 31 (1) ◽  
pp. 01-05
Author(s):  
Ahed A.H. Matloob ◽  
Hamid A A. Khafaji

The study aimed to evaluate the efficacy of the water extract of Propolis, Wild mustard and dates vinegar in the inhibition of Penicillium italicum causal agent of Blue mold on a lemon fruits. The results showed that all tested extracts with concentrations 5, 10 and 15% had a high inhibitory effect against P. italicum. Dates vinegar is highly effective and showed 100% inhibition of P. italicum and protected lemon fruit from infection. First time this type of natural inhibitory water extracts are used in Iraq which resulted that the dates vinegar is the best suitable option for the management of lemon blue mold disease in post-harvest.


2008 ◽  
Vol 5 (3) ◽  
pp. 330-333
Author(s):  
Baghdad Science Journal

The mutagenic effect of some crucifers widely consumed Lepiduim sativum (Garden cress) and Arugula (Eruca sativa) was studied in comparison to carrot (Daucus carota), using bacterial mutagenic system composed of three bacterial isolates; (Bacillus spp)G3 (Arthrobacter spp)G12, and (Brevibacterium spp)G27 ,. Treatment of isolates with plant extracts led to reduction in survival fraction (Sx)at different levels except that Aurgula extract did not show any inhibitory effect in isolates G12 and G27. Crucifers extracts induced streptomycin resistant mutants in G12 at higher level than G3 , but not in G27. No resistance to rifimpacin was detected in all isolates.


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