scholarly journals The Effect of the Different Artificial Insemination Time Periods on the Pregnancy Rate of Sapudi Ewes

2021 ◽  
Vol 11 (3) ◽  
pp. 469-473
Author(s):  
Cristy Dwi Putri ◽  
. Ismudiono ◽  
Emmanuel Djoko Poetranto

Brucellosis Artificial insemination is required to increase the reproduction rate in ruminant breeding. The artificial insemination success rate in sheep only reaches 47.6%, whereas the proposed ideal rate is 70%. One of the factors influencing the artificial insemination success rates in sheep is improper estrus detection, resulting in no fertilization. The present study aimed to determine the effect of different artificial insemination time periods on the pregnancy rates of Sapudi ewes. The research design was based on a completely randomized design. A total of 20 female Sapudi sheep were divided into four treatment groups with five repetitions (for each group). In addition, the observed variables were artificial insemination time in Sapudi ewes. Estrus synchronization in ewes was conducted by injecting PGF2α. The results of the research indicated that ewes subjected to artificial insemination 6, 12, 18, and 24 hours after estrus had a pregnancy rate of 20%, 100%, 60%, and, 60%, respectively. It can be concluded that the time differences in artificial insemination significantly influence the pregnancy rate in Sapudi ewes’.

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Mefrianti Efendi ◽  
Tongku Nizwan Siregar ◽  
Hamdan Hamdan ◽  
Dasrul Dasrul ◽  
Cut Nila Thasmi ◽  
...  

This study aims to know the effect of estrus synchronization using ovsynch method on local cow pregnancy rate. This study used 10 local cows without pregnant status and clinically healthy. All cows were divided into 2 treatment groups. Cows in group I (K1) were synchronized estrus using 5 mg/ml PgF2α intramuscularly with multiple injection patterns with intervals of 10 days. Group II (KII) was synchronized by ovsynch protocol, initiated with injection of 1ml GnRH on day-0. On the 7th day, the whole cows in K II were injected with 2 ml PgF2α then followed by re-injected with 1 ml of GnRH on the 9th day. Artificial insemination was performed 24 hours after GnRH last injection. The data were analyzed descriptively. The results showed that 3 cows in group I were diagnosed pregnant (60%), whereas in group II, 4 cows werediagnosed pregnant (80%). In conclusion, the estrus synchronization with ovsynch protocols provide a higher pregnancy rate than the conventional estrus synchronization.Key words: synchronization, ovsynch, GnRH, PgF2α


2020 ◽  
Vol 7 (2) ◽  
pp. 156
Author(s):  
Silvia Rani Andriyanti ◽  
Mas’ud Hariadi ◽  
Roesno Darsono ◽  
Pudji Srianto ◽  
Wurlina Wurlina ◽  
...  

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.


2017 ◽  
Vol 3 (6) ◽  
pp. 712
Author(s):  
Silvia Rani Andriyanti ◽  
Mas’ud Hariadi ◽  
Roesno Darsono ◽  
Pudji Srianto

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53.3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66.7%. Without treatment or control, sample with an average of (1.67±0.48) resulted in a  73.3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0.00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices. Keyword: estrus detection, artificial insemination, dairy cow, pregnancy rate


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 115-116
Author(s):  
Nicky Oosthuizen ◽  
Pedro Fontes ◽  
Ramiro Filho ◽  
Carl R Dahlen ◽  
David M Grieger ◽  
...  

Abstract To determine effects of presynchronization and delayed fixed-time artificial insemination (TAI) on pregnancy rates to TAI (PR/AI) with sex-sorted semen, 2,855 Bos taurus beef heifers were enrolled in a completely randomized design. Within location, heifers were randomly assigned to one of eight treatments: 1 and 2), heifers were exposed to the 7-d CO-Synch+CIDR protocol wherein they received gonadotropin-releasing hormone (GnRH) and a CIDR insert on d 0, prostaglandin F2α (PGF) upon CIDR removal on d 7, and were TAI 54 ± 2 h later with conventional (CTRL54-CNV; n = 359) or sex-sorted semen (CTRL54-SEX; n = 356); 3 and 4), same as CTRL54 but were TAI at 72 ± 2 h with conventional (CTRL72-CNV; n = 366) or sex-sorted semen (CTRL72-SEX; n = 360); 5 and 6), same as CTRL54 but also received PGF 7 d prior to initial injection of GnRH (d -7) and were TAI with conventional (PRE54-CNV; n = 355) or sex-sorted semen (PRE54-SEX; n = 353); 7 and 8), same as PRE54 but had TAI delayed to 72 ± 2 h and were inseminated with conventional (PRE72-CNV; n = 351) or sex-sorted semen (PRE72-SEX; n = 355). All heifers received estrus detection patches on d 7, which were evaluated for activation at TAI. Ultrasonography was performed 30–45 d after TAI to determine PR/AI. Estrus expression was greater (P < 0.01) in the CTRL72 heifers compared to CTRL54, PRE54, and PRE72 heifers (73.0 vs 55.4, 43.4, and 65.7%, respectively). Moreover, estrus expression was greater (P < 0.01) in PRE72 heifers compared to CTRL54 and PRE54 heifers. Within treatment pairs, PR/AI were greater (P ≤0.04) when conventional semen was utilized compared to sex-sorted semen. Furthermore, PR/AI were greater (P = 0.02) in PRE72-SEX heifers than CTRL54-SEX heifers (45.8 vs. 37.5%). No difference (P = 0.20) was determined in PR/AI between CTRL54-CNV and PRE72-SEX heifers (50.5 vs. 45.8%). In conclusion, presynchronization in combination with delayed TAI increased estrus expression and PR/AI with sex-sorted semen in replacement beef heifers.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 116-117
Author(s):  
Emily G Smith ◽  
Rachael C Bonacker ◽  
Carson M Andersen ◽  
Jordan M Thomas

Abstract Estrus detection patches were evaluated as a tool to classify postpartum cows as estrous cycling or anestrous at the start of the breeding season. EstrotectTM Breeding Indicators were applied to 257 postpartum beef cows in three locations 25 days prior to the start of estrus synchronization. Coincident with the start of estrus synchronization, patches were scored using a 1 to 4 scale (1 = 0–25%; 2 = 25–50%; 3 = 50–75%; 4 = 75–100%) with scores of 3 or 4 considered activated. Blood samples were collected from each cow 10 days prior to, and at the start of the estrus synchronization. Serum progesterone concentrations were determined via radioimmunoassay; cows were considered estrous cycling if one or both samples exceeded a concentration of 0.5 ng/ml. Based on progesterone, 54.9% of cows were estrous cycling at the start of estrus synchronization. When missing patches were considered inconclusive results, use of patches to infer estrous cyclicity resulted in 85.0% sensitivity and 79.2% specificity with a positive predictive value of 87.2%. However, 75.1% of all cows presented with missing patches at the start of synchronization. If missing patches were considered activated, sensitivity was 95.7%, but specificity (16.4%) and PPV (58.2%) were poor, as 47.6% of cows presenting with missing patches were anestrous based on progesterone. Across all cows, pregnancy rate to AI was 58.0% (149/257). Cows with activated patches that were classified as false positives based on serum progesterone concentrations achieved a 71.4% (5/7) pregnancy rate to AI, raising questions as to whether sensitivity was underestimated due to inherent Type II errors in classification of cyclicity via progesterone. In summary, estrus detection aids are a sensitive tool to identify estrous cycling postpartum cows prior to the start of the breeding season; however, poor specificity and patch retention are limitations for this application.


Author(s):  
Rinawati Rinawati ◽  
E Suharyanto ◽  
Nastiti Wijayanti

Tithonia diversifolia is a plant that has the potential to reduce blood glucose levels that is crucial in Diabetes Mellitus therapy. This study aims to determine the active compounds contained in the extract of T. diversifolia leaves decoction, the effect of decoction extract on the reduction of blood glucose, and the most effective decoction extract to reduce blood glucose levels as well. The study used a Completely Randomized Design (CRD) with the research treatment groups, namely: Kn = normal control (normal mice and not given leaves decoction extract), Ka = STZ control (DM mice, which are normal mice induced by 65mg/Kg of STZ), Kp = treatment control (normal mice given leaf decoction extract) , P1 = DM + mice were given young leaf decoction extract, P2 = DM + mice were given a mixture of young leaf and adult leaf decoction extract, P3 = DM + mice were given adult leaf decoction extract. The leaves used for decoction were 1-6 leaves from the shoots. The analysis of T. diversifolia leaves decoction compounds is using the visible spectrophotometers for tannins, phenols and flavonoids and GC-MS for terpenoid analysis. The result found that Tithonia diversifolia leaves decoction extracts contain tannins, flavonoids and phenols, while terpenoids are not detected. The leaves decoction extracts of T. diversifolia influenced the decreases in blood glucose levels of DM mice, especially the decoction extract of adult leaves which reduced blood glucose levels to 71.16%.


2020 ◽  
Vol 44 ◽  
Author(s):  
Neliane Galvão Porto ◽  
Felipe Barbosa Ribeiro ◽  
Jefferson Costa de Siqueira ◽  
Marcos Antonio Delmondes Bomfim ◽  
Rafael Silva Marchão ◽  
...  

ABSTRACT Methionine is an essential amino acid, and generally, the first limiting one in the diets of tambaqui, the main native fish produced in continental aquaculture in South America. However, there is a lack of information on their amino acid requirement, especially for maintenance and efficiency of utilization. The present study aimed to determine the requirement of [methionine plus cystine] for the maintenance and efficiency of utilization using tambaqui of different body weights. Two experiments were carried out using 250 tambaqui with weights of 100 and 300 g. They were distributed in a completely randomized design with four treatment groups (0.264, 0.396, 0.528, and 0.660% of digestible [Met + Cys]), five replications. The control treatment (CT) was performed with the addition of methionine at the first level (0.264%) until the second level was reached (0.396%). The maintenance requirement was determined by the regression analysis between the consumption of [Met + Cys] and protein retention equal to zero. The efficiency was determined using the value of the slope coefficient of the line obtained by the linear regression. The protein and methionine retention values of tambaqui weighing 100 to 300 g were compared by the parallelism test, and no difference was observed between the parameters of the equations indicating the need for only one equation to describe the responses to body weights. The requirement of digestible [Met + Cys] for the maintenance of tambaqui with weights from 100 to 300 g was 60.47 mg kg-0.7 day-1 and the efficiency of utilization of 42%.


2010 ◽  
Vol 10 (2) ◽  
pp. 21-26
Author(s):  
Sugito Sugito ◽  
Erdiansyah Rahmi ◽  
Azhari Azhari ◽  
M. Isa

The gain body weight and Spoilage of meat broiler which giving jaloh extract to combine with chromiumABSTRACT. A study was conducted on broiler chickens under heat stress to evaluate the effect of combined jaloh (Salix tetrasperma Roxb) extract with chromium mineral in the body weight, value of feed ratio conversion, and time to meat to become decomposed. Twenty four of 20-day old Cobb female broiler chickens were randomly assigned and divided to 4 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens given no heat stress and jaloh extract nor chromium (tCp); 2) chickens under heat stress without given jaloh extract nor chromium (Cp); 3) chickens under heat stress and given jaloh extract in 1.000 mg/lt drinking water (Cp+Ej); and 4) chickens under heat stress, given jaloh extract in 1.000 mg/lt drinking water, and chromium in 1.000 ug/lt drinking water (Cp+Ej+Cr). Heat stress given was 33 ± 1oC of cage temperature during 4 hours per day within 15 days. Jaloh extract and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. On the 16th day of the study chickens were measured before slaughtered. Samples obtained were chicken meat that taken from breast part (musculus pectoralis). The result suggested that either giving jaloh extract per se at the dose of 1.000 mg/lt in drinking water or combine it with chromium that given two hours before cage temperature reaching 33 ± 1oC, can prevent chickens from decreasing their body weight, decreasing value of feed ratio convertion, and extending time to meat to become decomposed.


2020 ◽  
Vol 47 (3) ◽  
pp. 124-131
Author(s):  
A. A. Imade ◽  
C. F. I. Onwuka ◽  
V. A. Jegede ◽  
O. Y. Ayo-Ajasa ◽  
B. B. Sarumi ◽  
...  

The need to seek for alternative feed materials with nutritional and medicinal values to support livestock growth necessitated this study. Forty-five unsexed rabbits crosses (Chinchilla × Newzealand white) 5- 6 weeks old with average weight of 720g were used in a 10- week feeding trial to evaluate the performance of growing rabbits fed on varying levels of dried okra leaves. The rabbits were assigned into five treatment groups containing dried okra leaves (DOL) at 0%, 2.5%, 5%, 7.5% and 10% inclusion levels for dietary treatments (T1, T2, T3, T4 and T5) respectively. Each treatment consisted of 9 rabbits and replicated thrice with each replicate consisting of 3 rabbits in a completely randomized design. Data collected were subjected to one-way analysis of variance (ANOVA). Results indicated that daily feed intake was significantly (P<0.05) largest in rabbits fed 5.00% inclusion level of dried okra leaves (84.57g) and least in rabbits fed 0.00% level of inclusion of dried okra leaves (72.12g). Feed efficiency (FE) was poorest (P<0.05) in rabbits fed 10.00% DOL (0.15%) and best (0.20%) in rabbits fed 2.50% dried okra leaves. It was concluded that okra leaves can be included in diets for growing rabbits and 2.50% level of dried okra leaves inclusion is recommended for adoption by farmers to maximize profit.


2018 ◽  
Vol 43 (4) ◽  
pp. 438
Author(s):  
S. Sutiyono ◽  
D. Samsudewa ◽  
A. Suryawijaya

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.


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