scholarly journals Expression of the aldehyde oxidase 3, ent-copalyl diphosphate synthase, and VIVIPAROUS 1 genes in wheat cultivars differing in their susceptibility to pre-harvest sprouting

2017 ◽  
Vol 15 (1) ◽  
pp. e0701 ◽  
Author(s):  
Magdalena Simlat ◽  
Michał Nowak ◽  
Kamil Brutkowski ◽  
Marcin Hydzik ◽  
Andrzej Zieliński ◽  
...  
Keyword(s):  
Genetic Background ◽  
Wheat Cultivar ◽  
Complex Trait ◽  
Aldehyde Oxidase ◽  
Acid Synthesis ◽  
Wheat Cultivars ◽  
Grain Development ◽  
Wheat Grains ◽  
Copalyl Diphosphate Synthase

The quality of wheat grains is often negatively affected by pre-harvest sprouting (PHS), a complex trait with a poorly understood genetic background. In this study two wheat cultivars differing in their susceptibility to PHS were used to investigate expression of three genes: AAO3, CPS3 and VP1. AAO3 is coding for aldehyde oxidase 3, an enzyme involved in the synthesis of abscisic acid. CPS3 codes for ent-copalyl diphosphate synthase which belongs to the pathway of gibberellic acid synthesis. The product of VP1 (VIVIPAROUS 1) is a transcription factor which controls expression of the former two genes. The study was carried out using both developing and sprouting-induced grains. In Piko, a wheat cultivar susceptible to PHS, accumulation of the AAO3 transcript was significantly decreased, during the last stages of grain development, in comparison to Sława, a cultivar tolerant to PHS. In case of the CPS3 and VP1 transcripts, the differences between cultivars were especially evident from 17th to 31st day after pollination. In turn, after induction of sprouting within spikes, accumulation of the AAO3 and VP1 mRNA in the Sława grains was lower in comparison to that observed in the Piko grains. Moreover, accumulation of the CPS3 transcript was significantly higher for Piko than for Sława, both in sprouting and non-sprouting grains. According to our knowledge this report provides the first description of the AAO3 and CPS3 expression in the context of PHS, and in the future it would be valuable to correlate this information with the data on the accumulation of ABA and GA3.

scholarly journals Programmed Degradation of Pericarp Cells in Wheat Grains Depends on Autophagy

2021 ◽  
Vol 12 ◽  
Author(s):  
Yong-Bo Li ◽  
Mei Yan ◽  
De-Zhou Cui ◽  
Chen Huang ◽  
Xin-Xia Sui ◽  
...  
Keyword(s):  
Regulatory Mechanisms ◽  
Grain Development ◽  
New Variety ◽  
Wheat Grains ◽  
Yield And Quality ◽  
Autophagy Gene ◽  
Cell Layers ◽  
Developmental Characteristics ◽  
Pericarp Thickness

Wheat is one of the most important food crops in the world, with development of the grains directly determining yield and quality. Understanding grain development and the underlying regulatory mechanisms is therefore essential in improving the yield and quality of wheat. In this study, the developmental characteristics of the pericarp was examined in developing wheat grains of the new variety Jimai 70. As a result, pericarp thickness was found to be thinnest in grains at the top of the spike, followed by those in the middle and thickest at the bottom. Moreover, this difference corresponded to the number of cell layers in the pericarp, which decreased as a result of programmed cell death (PCD). A number of autophagy-related genes (ATGs) are involved in the process of PCD in the pericarp, and in this study, an increase in ATG8-PE expression was observed followed by the appearance of autophagy structures. Meanwhile, following interference of the key autophagy gene ATG8, PCD was inhibited and the thickness of the pericarp increased, resulting in small premature grains. These findings suggest that autophagy and PCD coexist in the pericarp during early development of wheat grains, with both processes increasing from the bottom to the top of the spike. Moreover, PCD was also found to rely on ATG8-mediated autophagy. The results of this study therefore provide a theoretical basis for in-depth studies of the regulatory mechanisms of wheat grain development.

Abscisic Acid Synthesis and Metabolism in Wheat Ears

1979 ◽  
Vol 6 (1) ◽  
pp. 99 ◽  
Author(s):  
RW King
Keyword(s):  
Abscisic Acid ◽  
Grain Growth ◽  
Slow Rate ◽  
Acid Synthesis ◽  
Grain Development ◽  
Phaseic Acid ◽  
Wheat Grains ◽  
Physiological Importance ◽  
Dihydrophaseic Acid ◽  
Enhanced Degradation

During development of wheat grains their content of abscisic acid (ABA) increased, and this was also shown to occur in the grain of ears detached and grown in culture. Over a 10-day period of growth of ears in culture, their content of ABA increased from 0.31 to 2.56 ng/grain. Loss of ABA from the grain occurred on cessation of grain growth and drying of the grain. With loss of ABA, there was an associated but lesser accumulation of phaseic acid and dihydrophaseic acid, two known metabolites of ABA. Studies with [14C]abscisic acid indicated that premature drying of grain led to some conversion of ABA to compounds chromatographically similar to phaseic and dihydrophaseic acid, but the major products were more polar. Apparently, synthesis of ABA occurred in the developing grain (or at least in the ear) in association with a slow rate of degradation but, at maturity and drying of the grain, there was enhanced degradation of ABA. The physiological importance of these compounds in grain development and dormancy is discussed.

Transient changes in wheat endosperm polymeric proteins during the later stages of grain development

1995 ◽  
Vol 75 (1) ◽  
pp. 195-198 ◽  
Author(s):  
A. Hussain ◽  
O. M. Lukow
Keyword(s):  
Molecular Weight ◽  
Storage Proteins ◽  
Developmental Changes ◽  
Wheat Cultivars ◽  
Grain Development ◽  
Gluten Proteins ◽  
Distal Half ◽  
End Use ◽  
Qualitative Changes

End-use quality of wheat depends mainly on the composition of the storage proteins. These proteins undergo changes during grain development. This study was carried out to examine modifications in the polymeric protein fraction at different days post anthesis (DPA) and to determine whether these changes are cultivar dependent. Endosperm proteins of three hexaploid wheat cultivars harvested at different stages of maturity and extracted sequentially from distal half kernels showed quantitative and qualitative changes when separated in a 7.5–9.0% gradient polyacrylamide gel system. Sudden qualitative changes were observed at a later stage of seed development (35 DPA) in all tested cultivars. Most qualitative changes occurred in proteins of molecular weight 60–90 kDa and least in proteins above 90 kDa. An intense 60-kDa band appeared in the 35-DPA sample of Columbus; this band faded as the grain approached full maturity (40 DPA). Key words: Developmental changes, electrophoresis, gluten proteins, seed ripening

scholarly journals CORRELAÇÃO ENTRE O TEOR DE PROTEÍNAS EM GRÃOS DE TRIGO E A QUALIDADE INDUSTRIAL DAS FARINHAS

2002 ◽  
Vol 20 (1) ◽  
Author(s):  
LUIZ CARLOS GUTKOSKI ◽  
OTONI ROSA FILHO ◽  
CASSIANA TROMBETTA
Keyword(s):  
Protein Content ◽  
Wheat Flour ◽  
Chemical Constituents ◽  
Wheat Cultivars ◽  
Falling Number ◽  
Wheat Grain ◽  
Thousand Grain Weight ◽  
Dough Strength ◽  
Wheat Grains

Estudou-se o efeito da concentração de proteínas do grão de trigo na qualidade industrial das farinhas, bem como o estabelecimento de curvas de calibração mediante espectrofotometria de reflectância no infravermelho proximal (NIR). Amostras de grãos de trigo dos cultivares OR 1 e Rubi foram coletadas em regiões produtoras do sul do Brasil e realizadas análises de umidade, proteína bruta, peso de mil grãos, peso do hectolitro, grau de extração, número de queda, força do glúten (W), tenacidade (P), extensibilidade (L) e relação P/L. As farinhas das amostras de trigo do cultivar Rubi mostraram-se superiores quanto às características alveográficas avaliadas em comparação com as farinhas das amostras de trigo do cultivar OR 1. O teor de proteína bruta das amostras de grãos de trigo do cultivar Rubi apresentou correlação com a força geral do glúten (0,75) e relação P/L (-0,71). Os coeficientes de determinação das equações de proteína bruta (0,99) e umidade (0,95), obtidos nas curvas de calibração pelo NIR foram adequados para predizer a concentração dos constituintes químicos avaliados. CORRELATION BETWEEN PROTEIN CONTENT IN WHEAT GRAINS AND THE INDUSTRIAL QUALITY OF FLOURS Abstract The protein content of wheat grain in the industrial quality of flours was studied, as well as the development of calibration curves by NIR. Samples of wheat cultivars OR-1 and Rubi were collected in several regions and the analysis of moisture, protein content, thousand grain weight, hectoliter weight, flour extraction grade, falling number and alveography were undertaken. The wheat flour samples of the Rubi cultivar were superior for the alveography characteristics evaluated in comparison with the wheat samples of cultivar OR1. The protein content of wheat samples cultivar Rubi showed correlation with dough strength (0,75) and P/L ratio (-0,71). The determination coefficients for protein (0,99) and moisture (0,95) equations were adequate to predict the concentration of the chemical constituents evaluated.

Quality of Major Wheat Cultivars Grown in Sichuan Province in Recent Decade

2016 ◽  
Vol 42 (6) ◽  
pp. 803 ◽  
Author(s):  
Chao-Su LI ◽  
Xiao-Li WU ◽  
Yong-Lu TANG ◽  
Wu-Yun YANG ◽  
Yuan-Qi WU ◽  
...  
Keyword(s):  
Recent Decade ◽  
Sichuan Province ◽  
Wheat Cultivars

scholarly journals The Impact of Harvesting Time on Fusarium Mycotoxins in Spring Wheat Grain and Their Interaction with Grain Quality

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 642
Author(s):  
Yuliia Kochiieru ◽  
Audronė Mankevičienė ◽  
Jurgita Cesevičienė ◽  
Roma Semaškienė ◽  
Jūratė Ramanauskienė ◽  
...  
Keyword(s):  
Spring Wheat ◽  
Grain Quality ◽  
Fusarium Species ◽  
Triticum Aestivum L ◽  
Falling Number ◽  
Harvesting Time ◽  
Wheat Grains ◽  
The Impact ◽  
Full Maturity

In this work, we studied the impact of harvesting time on Fusarium mycotoxin occurrence in spring wheat and the effect of mycotoxin contamination on the quality of these grains. The spring wheat grains (Triticum aestivum L.) were collected in 2016–2018 when the crop had reached full maturity, 10 ± 2 days and 17 ± 3 days after full maturity. The grain samples were analyzed for Fusarium infection and co-contamination with mycotoxins deoxynivalenol (DON), zearalenone (ZEA), and T-2 toxin (T-2), as well as the quality of the wheat grains (mass per hectolitre, contents of protein, starch, ash and fat, particle size index (PSI), falling number, sedimentation, wet gluten content, and gluten index). The occurrence of Fusarium spp. fungi and the mycotoxins produced by them in the grains was mostly influenced by the harvesting time and meteorological conditions. The correlations between Fusarium species and the mycotoxins produced by them in the grains of spring wheat showed F. graminearum to be a dominant species, and as a result, higher concentrations of DON and ZEA were determined. The co-occurrence of all the three mycotoxins analyzed (deoxynivalenol, zearalenone, and T-2 toxin) was identified in wheat. In rainy years, a delay in harvesting resulted in diminished grain quality of spring wheat, as indicated by grain mass per hectolitre and falling number. Negative correlations were found in highly contaminated grains between mycotoxins (DON, ZEA, and T-2) and falling number and grain mass per hectolitre values.

scholarly journals Image data of crumb structure of bread from flour of czech spring wheat cultivars

2011 ◽  
Vol 22 (No. 4) ◽  
pp. 133-142 ◽  
Author(s):  
I. Švec ◽  
M. Hrušková
Keyword(s):  
Image Analysis ◽  
Image Data ◽  
Analysis Data ◽  
Wheat Cultivars ◽  
Rheological Measurements ◽  
Class A ◽  
Bread Volume ◽  
Class B ◽  
Crumb Structure

Abstract: Baking quality of flour from six wheat cultivars (harvest 2002 and 2003), belonging to the quality classes A and B, was evaluated using the fermented dough test. Analytical traits of kernel and flour showed differences between the classes which were confirmed by the baking test with the full-bread-formula according to Czech method. In addition to standard methods of the bread parameters description (specific bread volume and bread shape measurements) rheological measurements of penetrometer and image analysis were used in effort to differentiate wheat samples into the quality classes. The results of the baking test proved significant differences in specific bread volumes – the highest volume in class A was obtained with the cultivar Vinjet and in class B with SG-S1098 – approx. 410 and 420 ml/100 g. Although significant correlations among image analysis data and specific bread volume having been proved, any image analysis parameter did not distinguish the quality classes. Only the penetronetric measurements made with bread crumb were suitable for such purpose (r = 0.9083; for  = 0.01). Among image analysis data the total cell area of the crumb had the strongest correlation with specific bread volume (r = 0.7840; for α = 0.01).    

scholarly journals Compatibility of Azospirillum brasilense with fungicide and insecticide and its effects on the physiological quality of wheat seeds

2018 ◽  
Vol 39 (2) ◽  
pp. 855 ◽  
Author(s):  
Janete Denardi Munareto ◽  
Thomas Newton Martin ◽  
Tania Maria Müller ◽  
Ubirajara Russi Nunes ◽  
Guilherme Bergeijer da Rosa ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Seed Treatment ◽  
Accelerated Aging ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Pests And Diseases ◽  
Physiological Quality ◽  
Growth Promoting ◽  
Germination Vigor

Seed treatment is a practice that helps the initial establishment of the crop without the effects caused by pests and diseases. The association of diazotrophic bacteria with grasses has been used in the supply of nitrogen to plants; however, these microorganisms produce growth-promoting substances, which promote benefits in the growth and development of the crops. Thus, the objective of this study was to evaluate the compatibility of Azospirillum brasilense associated with the fungicide difenoconazole and the insecticide thiamethoxam by observing the effects on the quality of seed emergence of three wheat cultivars (Triticum aestivum L.). Three wheat cultivars, arranged in a 4 x 2 factorial system with four replicates, were tested. The treatments were the control; difenoconazole (Spectro® at a dose of 150 mL per 100 kg of seed); thiamethoxam (Cruiser® FS 350 at a dose of 200 mL per 100 kg seed) and difenoconazole + thiamethoxam, applied on wheat cultivars TBIO Mestre, TBIO Itaipu and TBIO Sinuelo. Bacteria from the Azospirillum genus were used in the inoculation. The wheat seed retains its quality when it checks the germination, vigor and independent accelerated aging, whether or not fungicide, insecticide and A. brasilense were used. The insecticide thiamethoxam increased the length of shoots and roots and provided compatibility with A. brasilense, and fungicide inhibited the length of shoots and roots and was antagonistic to the bacterium A. brasilense.

The relationship among freezing tolerance, vernalization requirement, Ppd alleles and winter hardiness in European wheat cultivars

2017 ◽  
Vol 155 (9) ◽  
pp. 1353-1370 ◽  
Author(s):  
A. GORASH ◽  
R. ARMONIENĖ ◽  
Ž. LIATUKAS ◽  
G. BRAZAUSKAS
Keyword(s):  
Molecular Markers ◽  
Western Europe ◽  
Complex Trait ◽  
Winter Hardiness ◽  
Photoperiod Sensitivity ◽  
The Other ◽  
Wheat Cultivars ◽  
Crucial Component ◽  
Per Se ◽  
The Relationship

SUMMARYWinter hardiness of wheat is a complex trait involving a system of structural, regulatory and developmental genes, which interact in a complex pathway. The objective of the present work was to study the relationship among the main traits determining the level of adaptation and the possibility for target manipulation of breeding material by using molecular markers and phenological parameters. Wheat cultivars from different ecoclimatic environments of Europe were included for analysis. Gene-specific assay showed that photoperiod sensitivity of the studied cultivars was determined by polymorphism in the Ppd-D1 allele. The study established the relationship among winter hardiness, LT50 (the temperature at which 50% of plants are killed), photoperiod sensitivity, vernalization duration and earliness per se genes in the environment of Lithuania. The cultivars from Northern and Western Europe exhibited stronger requirement for vernalization and photoperiod. Although the group of cultivars from the southern latitudes were characterized by earliness, they possessed a stronger level of LT50. The level of LT50 was found to be the most crucial component of winter hardiness, the other traits served as supplementary components.

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