scholarly journals Fermentative losses and chemical composition and in vitro digestibility of corn grain silage rehydrated with water or acid whey combined with bacterial-enzymatic inoculant

2021 ◽  
Vol 42 (6) ◽  
pp. 3497-3514
Author(s):  
Fernanda Naiara Fogaça da Cruz ◽  
◽  
Flávio Pinto Monção ◽  
Vicente Ribeiro Rocha Júnior ◽  
Amanda Maria Silva Alencar ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Free Water ◽  
Mean Value ◽  
In Vitro Digestibility ◽  
Lactobacillus Curvatus ◽  
Randomized Design ◽  
Aerobic Stability ◽  
Acid Whey ◽  
Corn Grain

This study aimed to evaluate the effects of rehydration with water or acid whey, as well as the effects of bacterial-enzymatic inoculant on fermentation losses, aerobic stability, and chemical composition and in vitro digestibility of corn grain silages. The treatments consisted of corn kernels (Zea mays) ground through a 3-mm sieve and rehydrated with chlorine-free water or unsalted whey (NaCl) combined or not with bacterial-enzymatic inoculant. This was a 2 x 2 factorial completely randomized design with sources of rehydration (water or whey) and absence and presence of bacterial-enzymatic inoculant with eight replications. Water and acid whey were added to ground corn with 12% moisture in order to increase it to 35%. Rehydrated corn was ensiled in Polyvinyl chloride (PVC) minisilos and stored for 60 d. The bacterial-enzymatic inoculant used was composed of Lactobacillus curvatus, L. acidophilus, L. plantarum, L. buchneri, L. lactis, Pediococcus acidilactici, Propionibacterium acidipropionici and Enterococcus faecium, in concentrations of 1010 CFU g-1 and 5% cellulose-based enzyme complex. The aerobic exposure of silages was evaluated for 120 hours. There was interaction (P = 0.02) between the sources of rehydration and the use or not of the inoculant on the values of silage temperature during aerobic exposure. There was a difference for dry matter (DM) losses (P=0.38) between rehydrated corn silages with and without inoculant. DM losses were 37.51% higher in silage without inoculant compared to silage with inoculant (3.84% DM). There was no difference between the sources of rehydration on DM losses (P = 0.39), with a mean value of 4.99%. Corn rehydration using acid whey (P < 0.01) increased 2.19% and 31.36% DM and ash content compared to water, an average of 66.14% and 1.28%, respectively. There was no interaction (P = 0.30) between the sources of rehydration and the use of inoculants on the in vitro digestibility of DM (mean of 79.26%). The use of the bacterial-enzymatic inoculant improved the fermentation characteristics, aerobic stability and nutritional value of corn grain silage rehydrated with water or whey.

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

scholarly journals Influence of the period of year on the chemical composition and digestibility of pasture and fodder selected by goats in caatinga

2017 ◽  
Vol 18 (3) ◽  
pp. 402-416 ◽  
Author(s):  
Messias José dos Santos SILVA ◽  
Dulciene Karla de Andrade SILVA ◽  
André Luiz Rodrigues MAGALHÃES ◽  
Kedes Paulo PEREIRA ◽  
Érica Carla Lopes da SILVA ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Semiarid Region ◽  
Mineral Matter ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Total Carbohydrates ◽  
Completely Randomized Design

SUMMARY This study aimed to verify the influence of months on chemical composition and in vitro digestibility of pasture and forage selected by goats in the semiarid region of Brazil’s Pernambuco state. Six male goats fistulated in the rumen were used in a completely randomized design. Forage collection was conducted in herbaceous and shrub/tree layers, in order to simulate goat grazing, and materials were separated into leaves and branches. Extrusa and pasture collections were held in November, January and February/March. There was collection month (P <0.05) effect for pasture on dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), with average values of 458.27g/kg NM and 155.46, 521.30 and 390.13g/kg DM, respectively. For the stem (P <0.05) for DM, Organic matter (OM), mineral matter (MM), ethereal stratum (EE), ADF, acid detergent unavailable protein (AIP), total carbohydrates (TCHO) and dry matter in vitro digestibility (DMIVD), with average values of 487.47g/kg NM and 930.80, 69.07, 20.67, 440.30 and 63.78g/kg DM, respectively. About the extrusa, there was collection time effect (P <0.05) on the DM, OM, MM, CP, EE, AIP, TCHO, Non-fibrous carbohydrates (NFC) and DMIVD, with average values of 84.53g/kg DM, 863.03, 136.97, 152.90, 33.60, 14.07, 676.37 and 55.35g/kg DM, respectively. Rainfall changes in the rainy season in the caatinga altered pasture quality, causing changes in the diet selected by goats, mainly in DM, CP, CNF and DMIVD.

scholarly journals Effect of particle size on storage time of hydrated corn grain

2021 ◽  
Vol 42 (3) ◽  
pp. 1189-1208
Author(s):  
Wilson Nei Zacaron ◽  
◽  
Clovisnei Basi ◽  
Cintiamara Baggio ◽  
Gabriela Solivo ◽  
...  
Keyword(s):  
Particle Size ◽  
Protein A ◽  
Maximum Temperature ◽  
Randomized Design ◽  
Aerobic Stability ◽  
Effect Of Particle Size ◽  
Corn Kernels ◽  
Storage Times ◽  
Corn Grain

Dry ground corn kernels are widely used as sources of energy readily available for microbial synthesis in the rumen. The objective of the present study was to measure the effect of particle size on the storage length of rehydrated and ensiled corn grain. Commercial corn was milled in 2-, 4-, 6-, 8-, and 10-mm sieves, rehydrated until reaching 65% DM, and ensiled for 14, 28, and 56 days, in a completely randomized design with a 5 x 3 factorial arrangement, with six replications per treatment. PVC mini-silos were used, with dimensions of 25 cm height x 10 cm of diameter. The variables DM, MM, CP, NDF, ADF, pH, molds and yeasts count, fermentative losses, in vitro degradability and aerobic stability were subjected to analysis of variance. The Tukey test was used to compare means. LAB counts was subjected to Wilcoxon's non-parametric test, considering significance at p < 0.05. Rehydrated corn silages with smaller particles (2 and 4 mm) showed higher LAB and mold counts, higher levels of CP and EE and higher density at opening silos than that of coarser particles. However, they showed lower percentages of DM, and were susceptible to microbial attack and faster temperature increases than were coarse particles during the chosen storage times. Silage subjected to 56 days of fermentation showed lower contents of NDF, ADF and CP, lower gas losses and lower pH values in aerobiosis, and increased levels of soluble protein (A + B1 fractions), losses by effluents, number of hours to reach maximum temperature, and time to break aerobic stability. Coarse-grind silages subjected to 56 days of storage showed better chemical composition, lower LAB and mold counts, and less susceptibility to microbial degradation when exposed to air.

scholarly journals Temporal Variations in Chemical Composition, In Vitro Digestibility, and Metabolizable Energy of Plant Species Browsed by Goats in Southern Mediterranean Forest Rangeland

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1441
Author(s):  
Youssef Chebli ◽  
Samira El Otmani ◽  
Mouad Chentouf ◽  
Jean-Luc Hornick ◽  
Jean-François Cabaraux
Keyword(s):  
Chemical Composition ◽  
Plant Species ◽  
Nutritive Value ◽  
Condensed Tannin ◽  
Metabolizable Energy ◽  
Mediterranean Forest ◽  
In Vitro Digestibility ◽  
Large Variability ◽  
Southern Mediterranean

Forest rangelands contribute largely to goat diets in the Mediterranean area. Information about browsed plant quality is essential for adequate feeding management. The purpose of this study was to evaluate the temporal changes in chemical composition and in vitro digestibility of the main plant species selected by goats in the Southern Mediterranean forest rangeland during two consecutive years; these were very contrasted (dry and wet). The browsed species were composed of herbaceous, eleven shrubs, and four tree species. Overall, large variability in chemical composition, in vitro organic matter digestibility (IVOMD), and metabolizable energy (ME) was observed among species, grazing season (spring, summer, and autumn), and years within each species. Crude protein (CP) content varied from 60 to 240 g/kg dry matter (DM). The fiber fractions, except for Quercus suber, increased significantly by advancing maturity. Due to the water stress, the lignin level presented a higher value during the spring of the dry year. Condensed tannin (CT) content varied from 2 to 184 g/kg DM. CP, IVOMD, and ME showed a negative correlation with lignin and CT. Based on the results presented herein, it is concluded that the nutritive value of the browsed plant species was highest in the spring and lowest during the summer and autumn of both studied years. With a good grazing management strategy, the selected plant species by goats could guarantee high-quality feeding resources throughout the year.

scholarly journals Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chatchai Kaewpila ◽  
Pongsatorn Gunun ◽  
Piyawit Kesorn ◽  
Sayan Subepang ◽  
Suwit Thip-uten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Acid Fermentation ◽  
Design Factor ◽  
Forage Sorghum ◽  
Randomized Design

AbstractImproving the nutrition of livestock is an important aspect of global food production sustainability. This study verified whether lactic acid bacteria (LAB) inoculant could promote ensiling characteristics, nutritive value, and in vitro enteric methane (CH4) mitigation of forage sorghum (FS) mixture silage in attacking malnutrition in Zebu beef cattle. The FS at the soft dough stage, Cavalcade hay (CH), and cassava chip (CC) were obtained. The treatments were designed as a 4 × 2 factorial arrangement in a completely randomized design. Factor A was FS prepared without or with CH, CC, and CH + CC. Factor B was untreated or treated with Lactobacillus casei TH14. The results showed that all FS mixture silages preserved well with lower pH values below 4.0 and higher lactic acid contents above 56.4 g/kg dry matter (DM). Adding LAB boosted the lactic acid content of silages. After 24 h and 48 h of in vitro rumen incubation, the CC-treated silage increased in vitro DM digestibility (IVDMD) with increased total gas production and CH4 production. The LAB-treated silage increased IVDMD but decreased CH4 production. Thus, the addition of L. casei TH14 inoculant could improve lactic acid fermentation, in vitro digestibility, and CH4 mitigation in the FS mixture silages.

PSXIV-14 Supplementation of high-grain diet with red osier dogwood affects in vitro digestibility and fermentation characteristics

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 477-477
Author(s):  
Wenzhu Yang
Keyword(s):  
Control Diet ◽  
Antimicrobial Properties ◽  
Positive Control ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Rumen Acidosis ◽  
Barley Silage ◽  
And Control

Abstract Red osier dogwood (ROD) is a native shrub plant rich in phenolic compounds with antimicrobial properties. The objective of this study was to evaluate the effects of substituting barley silage with either raw ROD or ROD extract (RODE) in high-grain (HG) diet under a low media pH (5.8) on gas production (GP), dry matter (DM) disappearance (DMD) and fermentation characteristics in batch cultures. The study was a completely randomized design with 4 treatments: 1) control diet (10% barley silage and 90% barley concentrate, DM basis), control diet supplemented with 2) monensin (30 mg/kg diet DM; positive control), 3) substitution of 3% ROD or 4) 3% RODE for an equal portion of silage. Inoculum was obtained from 2 ruminally fistulated beef heifers offered the HG diet. Substrate ground (1 mm) was incubated for 24 h and the experiment repeated twice. The GP did not differ among treatments (147 ml/g DM), but the DMD differed (P &lt; 0.02) at highest for control (69.4%), lowest for ROD (58.4%) and intermediate for other treatments (64.1%). Total volatile fatty acid (VFA) concentration (mM) tended (P&lt; 0.08) to be lower with ROD (80.5) and monensin (80.1) than control (83.9). Acetate proportion was greater (P = 0.02) with ROD (46.2%) and RODE (46.9%) than control (42.4%) and monensin (42.3%). However, the propionate proportion was greater (P = 0.05) with monensin (32.1%) than other treatments (averaged 30.1%). Consequently, acetate to propionate ratio (A:P) of ROD (1.52) and RODE (1.56) was higher than monensin (1.32; P &lt; 0.01) and control (1.44; P &lt; 0.08). Differences in variables measured between ROD and RODE were minimal. These results indicated that the decreased DMD along with increased A:P with addition of ROD or RODE suggests that both ROD and RODE may be beneficial to HG fed cattle for reducing risk of rumen acidosis without negatively impacting fibre digestion.

scholarly journals Nutrient Quality and in Vitro Digestibility of Bio-Ammoniated Rice Straw Bales

2020 ◽  
Vol 44 (3) ◽  
Author(s):  
Cuk Tri Noviandi ◽  
Dibya Ratnopama ◽  
Ali Agus ◽  
Ristianto Utomo
Keyword(s):  
Rice Straw ◽  
Crude Protein ◽  
Production Method ◽  
Proximate Analysis ◽  
Gas Production ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Nutrient Quality ◽  
Randomized Design

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.

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