scholarly journals Synthesis, characterization and evaluation of antibacterial activity of copper(II)-curcumin complex against staphylococcus aureus

2021 ◽  
pp. 52-57
Author(s):  
Le Dang Khoa ◽  
Hoang Minh Hao
Keyword(s):  
Staphylococcus Aureus ◽  
Ground State ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Methods ◽  
Chelating Ligands ◽  
Step Procedure ◽  
Inhibition Zones ◽  
Low Levels ◽  
Synthetic Approaches

Curcumin, a phytochemical from turmeric, and its derivatives have been extensively investigated from both chemical and biological strategies. However, the main problem encountered while using curcumin in clinical trials is its poor solubility and rapid degradation, resulting in its low levels in tissues, thus decreasing the medicinal effect of curcumin. To overcome these problems several synthetic approaches have been carried out to prepare new derivatives possessing better properties. Curcumin as a β-diketone ligand can act as chelating ligands toward a variety of metals to form stable complexes. Some studies showed that a metal-curcumin complex displayed potential medicinal activities. In this work, a copper(II)-curcumin complex was synthesized in a two-step procedure: (i) curcumin was separated from commercial turmeric powder using chromatography techniques and (ii) the copper(II) chloride (1 eq.) and pure curcumin (2 eq.) were mixed together in ethanol. The mixture was stirred at 60 oC for 3 hours to afford a stoichiometric copper(II)-curcumin complex.  The curcumin ligand and its copper(II) complex were characterized by UV-Vis, IR, NMR spectroscopic methods, from which it was found that copper atoms are coordinated through keto-enol groups of two curcumin molecules. The ground state spectral features of the copper(II)-curcumin complex were consistent with that of the 1:2 copper(II)-curcumin complex. The antibacterial activities of curcumin ligand and its complex were evaluated against Staphylococcus aureus (ATCC 6538) using a well diffusion method on nutrient agar. The results showed that the inhibitory activity was not observed for free curcumin at any concentrations while the copper(II)-curcumin complex exhibited the inhibition zones (mm) of 7.8, 11.6 and 14.9 at various concentrations (mg/mL) of 1.0, 5.0 and 15.0, respectively.  

scholarly journals ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES

2017 ◽  
Vol 9 (10) ◽  
pp. 276 ◽  
Author(s):  
Gouse Basha Sheik ◽  
Muazzam Sheriff Maqbul ◽  
Gokul Shankar S. ◽  
Ranjith M S
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Streptomyces Sp ◽  
Crude Extracts ◽  
Test Organisms ◽  
Inhibition Zones

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteriaMethods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods.Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm).Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

scholarly journals Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties

2020 ◽  
pp. 10-22
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Nano Particles ◽  
Diffusion Method ◽  
Adansonia Digitata ◽  
Inhibition Zones

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.

scholarly journals Chemical composition, antibacterial activity and related mechanism of valonia and shell from Quercus variabilis Blume (Fagaceae) against Salmonella paratyphi a and Staphylococcus aureus

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Dan Zhou ◽  
Ze-Hua Liu ◽  
Dong-Mei Wang ◽  
Deng-Wu Li ◽  
Li-Na Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antibacterial Mechanism ◽  
Natural Antimicrobials ◽  
Butanol Fraction ◽  
Quercus Variabilis ◽  
Crude Extracts

Abstract Background Plant secondary metabolites and phytochemicals that exhibit strong bioactivities have potential to be developed as safe and efficient natural antimicrobials against food contamination and addressing antimicrobial resistance caused by the overuse of chemical synthetic preservative. In this study, the chemical composition, antibacterial activities and related mechanism of the extracts of the valonia and the shell of Quercus variabilis Blume were studied to determine its potential as a safe and efficient natural antimicrobial. Methods The phenolic compositions of valonia and shell extracts were determined by folin-ciocalteau colourimetric method, sodium borohydride/chloranil-based assay and the aluminium chloride method and then further identified by the reverse-phase HPLC analysis. The antibacterial activities of valonia and shell extracts were evaluated by the agar disk diffusion method and agar dilution method. The related antibacterial mechanism was explored successively by the membrane of pathogens effect, phosphorous metabolism, whole-cell proteins and the microbial morphology under scanning electron microscopy. Results The n-butanol fraction and water fraction of valonia along with n-butanol fraction of the shell contains enrich phenolics including ellagic acid, theophylline, caffeic acid and tannin acid. The n-butanol fraction and ethanol crude extracts of valonia exhibited strong antibacterial activities against Salmonella paratyphi A (S. paratyphi A) and Staphylococcus aureus (S. aureus) with the DIZ values ranged from 10.89 ± 0.12 to 15.92 ± 0.44, which were greater than that of the Punica granatum (DIZ: 10.22 ± 0.18 and 10.30 ± 0.21). The MIC values of the n-butanol fraction and ethanol crude extracts of valonia against S. paratyphi A and S. aureus were 1.25 mg/ml and 0.625 mg/ml. The related antibacterial mechanism of n-butanol fraction and ethanol crude extracts of valonia may be attributed to their strong impact on membrane permeability and cellular metabolism. Those extracts exhibited strong antibacterial activity according to inhibit the synthesis of bacterial proteins and seriously change morphological structure of bacterial cells. Conclusions The n-butanol fraction and ethanol crude extracts of valonia had reasonably good antibacterial activities against S. paratyphi A and S. aureus. This study suggests possible application of valonia and shell as natural antimicrobials or preservatives for food and medical application.

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

2017 ◽  
Vol 901 ◽  
pp. 124-132
Author(s):  
Artania Adnin Tri Suma ◽  
Tutik Dwi Wahyuningsih ◽  
Deni Pranowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Shigella Flexneri ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

2003 ◽  
Vol 9 (1) ◽  
pp. 49-51
Author(s):  
Farida Lanawati Darsono ◽  
Stephanie Devi Artemisia
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Traditional Medicine ◽  
Tween 80 ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Gram Positive Bacteria ◽  
Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

scholarly journals Antibacterial activities of combination of ethanol extract of Delima fruit (Punica granatum L.) and Sereh stem (Cymbopogon citratus) on Staphylococcus aureus

2019 ◽  
Vol 6 (12) ◽  
pp. 5037
Author(s):  
Melia Pebrina ◽  
Indah Komala Sari ◽  
Eliza Arman ◽  
Honesty Diana Morika
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Pomegranate Peel Extract

ABSTRACT Background: Pomegranates contain chemical saponins and flavonoids while lemongrass stems have flavonoid chemical compounds. Substances that can inhibit the linking of bacteria are saponins and flavonoids. The most common microorganism found in ulcus diabetikum (ulcer diabetes) is Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of a combination of pomegranate ethanol extract and ethanol extract of lemongrass stems against S. aureus bacteria.Methods: This study used an experimental design with a complete randomized design study divided into 5 groups: groups 1 (75:25), 2 (50:50), 3 (25:75), positive control (tetracycline) and negative control. Manufacture of pomegranate peel extract and lemongrass stems was done by maceration method for further rotary, after the extract was obtained tested for antibacterial activity by diffusion method using a cylinder. With the test used analysis of variance one way.Results: The results of this study showed that the combination of pomegranate ethanol extract and citronella stem ethanol extract showed activity against S. aureus with a ratio of 15 ul:5 ul, 15 ul:15 ul, 5 ul:15 ul with inhibitory diameter of  respectively 13 mm, 11 mm, 8 mm. Whereas for positive control with chloramphenicol, the inhibition area is 9 mm.Conclusions: Statistically the combination of pomegranate ethanol extract and lemongrass stems has antibacterial power which uses p=0.005 and p<0.05. The combination of pomegranate ethanol extract and lemongrass stems has very strong antibacterial activity against S. aureus due to inhibition zones of 10-20 mm.

scholarly journals Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus

2021 ◽  
Vol 4 (1) ◽  
pp. 56
Author(s):  
Shinta Levea Ni'matul Fadlilah ◽  
Mustofa Helmi Effendi ◽  
Wiwiek Tyasningsih ◽  
Lucia Tri Suwanti ◽  
Jola Rahmahani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Cinnamon Bark ◽  
Cinnamomum Burmannii

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on  MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.

scholarly journals Antibacterial Screening of some Heterocyclic Compounds Containing Quinoline Nucleus

2017 ◽  
Vol 76 ◽  
pp. 27-40
Author(s):  
Shipra Baluja ◽  
Nilesh Godvani ◽  
Sumitra Chanda
Keyword(s):  
Heterocyclic Compounds ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Methods ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
H Nmr ◽  
Antibacterial Screening ◽  
Derivatives Of ◽  
Agar Well Diffusion Method

In this work, some novel derivatives of Cyanopyridines and Isoxazoles were synthesized using Vilsmeier-Haack reagent and their structures were confirmed by FTIR, 1H NMR and mass spectroscopic methods. The antibacterial activities of these synthesized compounds were studied in DMSO and DMF by agar well diffusion method against some Gram positive and Gram negative bacteria. It is observed that activity depends upon three S: solvent, strain and structure.

scholarly journals The Effect of Temperature and Heating Time of Roselle Extract (Hibiscus Sabdariffa L) Powder Solution on Growth Inhibition of Staphylococcus aureus ATCC 25923

2021 ◽  
Vol 8 (2) ◽  
pp. 55
Author(s):  
Hana Sofiana Maghfira ◽  
Isnaeni Isnaeni ◽  
Asri Darmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Block Design ◽  
Heating Time ◽  
Bacterial Activity ◽  
Effect Of Temperature ◽  
Diffusion Method ◽  
Hibiscus Sabdariffa ◽  
Control Block ◽  
Significant Difference ◽  
Inhibition Zones

The main ingredients of roselle (Hibiscus sabdariffa L.) which have antioxidant and antibacterial effect were anthocyanins, and flavonoids. Flavonoid compounds were unstable at high temperatures. Meanwhile, the processing of roselle extract powder into ready-to drink or food preparations generally requires the thermal processes. The aims of this study were to determine the effect of temperature and heating time of roselle extract powder solution on the inhibition of microbial growth of Staphylococcus aureus ATCC 25923. The method of this study uses a ready-to- process roselle powder extract sample. The roselle extract powder solution in a screw cap tube was heated at a temperature of 40°C, 50°C, 60°C, 70°C, 80°C, and 90°C on a waterbath, with heating times at each temperature were 15 minutes and 30 minutes. Anti bacterial activity of the sample solution was measured based on the ability to inhibit the growth of Staphylococcus aureus ATCC 25923 by the agar-well diffusion method. The inhibition zone of the sample was measured by caliper. The result of this study showed that inhibition zones caused by the sample that had been heated at those temperature for 15 minutes were 9.55±0.70 mm, 9.53 ±0.04 mm, 9.70±0.14 mm, 9.68±0.24 mm, 10.10±0.14 mm, and 10.25±0.21 mm. Meanwhile, after heating for 30 minutes the inhibition zones were 10.08±0.25 mm, 10.20±0.28 mm, 10.43±0.18 mm, 10.08±0.18 mm, 10.78±0.04 mm, 9.70±0.14 mm. Two ways statistical test ANOVA Randomized Control Block Design with 95% confidence level was used to prove that there was a significant difference berween the mean inhibition zones of each sample. The conclution of this research was, the temperature in the range of 40 -90 oC has no effect on the anti-bacterial activity of the sample. However, the heating time of the sample affected the antibacterial activity of the roselle extract powder solution against Staphylococcus aureus ATCC 25923.

scholarly journals Antibacterial effect of nettle (Urtica dioica)

2014 ◽  
Vol 13 (1) ◽  
pp. 1 ◽  
Author(s):  
N.A. Salih
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Salmonella Spp ◽  
Gram Positive Bacteria ◽  
Ethanol Extracts ◽  
Klebsiella Spp

The antibacterial activity of aqueous and 95% ethanol extracts of nettle leaf was tested against some Gram-negative and Gram-positive bacteria isolated from hospitalized patients by the agar well diffusion method. Staphylococcus aureus, Escherichia coli, Klebsiella spp., Bacillus Subtillus, Proteus spp. Salmonella spp. and Pseudomonas spp. were used. The results indicate that both extracts showed different antibacterial activities which were in favor of ethanolic extract because of more solubility of active ingredient in ethanol than in water. Staphylococcus aureus, Bacillus Subtillus, and Salmonella spp. showed the highest susceptibility to nettle extracts antibacterial effect, while E coli, Pseudomonas and Proteus were less susceptible. The only clear resistant bacteria isolate was Klebsiella spp.

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