The effectiveness of clove oil, its fractions, and clove oil-based fumigant tablet formulations, against Tribolium castaneum (Herbst)

2021 ◽  
Vol 18 (3) ◽  
pp. 191-198
Author(s):  
Trijanti Anughrahiyaharti Widinni Asnan ◽  
◽  
Sri Widayanti Widayanti ◽  
Idham Sakti Harahap ◽  
Herni Widhisatuti ◽  
...  

Indonesia has seen an increase and widespread reports of resistance among stored-product insect pests to phosphine. The use of Syzigium aromaticum (clove oil) as an alternative fumigant may be a useful strategy to control infestation by phosphine-resistant varieties of stored-product insects. The objective of this study was to examine the effectiveness of whole (unfractionated) clove oil as well as its component fractions as a fumigant and repellent against the red flour beetle (Tribolium castaneum (Herbst)), and to develop a simple fumigant formulation for this purpose. The experimental design used to test the effectiveness of clove oil and its fractionation was a completely randomized design (CRD). Meanwhile, testing the effectiveness of tablet formulations was carried out by factorial CRD. Fumigation test results gave LD50 and LD95 values of 0.234 and 1.142 ml/l respectively, for crude clove oil used in a fumigation chamber against T. castaneum. An n-hexane fraction of clove oil tested under the same conditions was more lethal, causing 95% mortality of T. castaneum at the dose of 0.801 ml/l during fumigation. Finally, tablets containing a set proportion of clove oil and naphthalene (1:1) reached LD91 against T. castaneum after 7 days’ fumigation.

2021 ◽  
Vol 10 (1) ◽  
pp. 46-53
Author(s):  
Yulia Pujiastuti ◽  
Erni Indriani ◽  
A Muslim ◽  
Chandra Irsan ◽  
Arsi Arsi

Bacillus thuringiensis is an entomopathogenic bacterium isolated from the soil and  has been widely used as an active ingredient in the manufacture of bioinsecticides. The target insects are very specific and depend on the type of protein content. Epilachna sp. are important insect pests because both larvae and adults as plant-eating pests. The research aimed was to investigate the effectivity  of  B. thuringiensis against the larvae of Epilachna sp.. B. thuringiensis- bio-insecticide was prepared using isolates originally from freshwater swamp soil of South Sumatra (SMR04). Epilachna sp larvae were mass-reared with Solanum torvum leaf feed in the laboratory. The design used was a completely randomized design, with 6 treatments and 5 replications. Treatments were spore concentration contained in the bioinsecticide solution included: 1 x 108, 1 x 107, 1 x 106, 1 x 105 spores/mL, commercial bio-insecticide and without treatment as a control. Each replication used 10 individual of the 2nd larvae. Statistical test results showed larval mortality in B. thuringiensis treatment was significantly different from commercial bio-insecticide treatment. The highest mortality of bio-insecticide treatment occurred at a concentration of 108 spores/ml (40.00 %) and the lowest was at a concentration of 105 spores/mL (18.01 %). The lowest LT50 value of bio-insecticide treatment was at a concentration of 108 spores/mL, namely 79.37 hours. The control of larvae included in the Coleoptera order was still not satisfactory yet, considering the presence of protein content in B. thuringiensis strain SMR04 which did not match the type of protein required.


Author(s):  
Ayi Yustiati ◽  
Syakirah Imtinan Zurwana ◽  
Achmad Rizal ◽  
Yuli Andriani

The purpose of this research is to determine the optimal dose of the addition of red spinach powder to artificial feed on the brightness of clown loach. This research was conducted at Aquaculture Laboratory Building 4 Faculty of Fisheries and Marine Sciences of Universitas Padjadjaran from April to May 2020. This research method is experimental with a Completely Randomized Design consisting of four treatments and three replications. The red spinach powder addition treatment used 0%, 2%, 4%, and 6% of the feed amount. The parameters observed are color value as primary data by using Toca Color Finder, while the growth, survival rate, and water quality as support data. Color assessment results were analyzed using the Kruskal-Wallis test, if there were significant differences, Z test would be performed. Growth data and survival rates were analyzed using Analysis of Variance (ANOVA). Because the F test results were not significantly different, the Duncan test was not carried out. The results showed that the addition of  6% red spinach powder is the best treatment with an increased color brightness value of 5,63.


2021 ◽  
Vol 20 (3) ◽  
pp. 556
Author(s):  
Muhammad Mustarsidin ◽  
Syachruddin AR ◽  
Ahmad Raksun

Plants that can be used as pesticides are Gadung tubers. Gadung tubers contain cyanide acid to kill armyworm pests because they contain dioscorin toxins which can cause nervous disorders. The purpose of this study was to determine the effect of Gadung tuber extract (Dioscorea hispida) on Caterpillar mortality (Spodoptera exigua) in Shallot plants. This type of research is experimental research. This study used a completely randomized design (CRD). The sample used was 5 caterpillars (Spodoptera exigua) in each polybag with 5 repetitions for each concentration of Gadung tuber extract, namely 0%, 0.5%, 1.0%, 1.5%, 2.0% and 2.5%, so the total Caterpillar used was 150 Caterpillars. Gadung tuber extraction was carried out by maceration method with methanol solvent. Data were analyzed using a one-way analysis of variance at the 95% significance level, followed by DMRT (Duncans Multiple Range Test). The results showed that the Gadung tuber extract had a significant effect on the mortality of caterpillars (Spodoptera exigua) because the F count was greater than the F table (45.40>2.62), and the DMRT test results showed that the optimal concentration of Gadung tuber extract was 2.5%. Therefore, it can be concluded that the extract of Gadung tuber (Dioscorea hispida) affects the mortality of Caterpillars (Spodoptera exigua) in Shallots (Allium cepa).


2019 ◽  
Vol 5 ◽  
pp. 115
Author(s):  
Syahri Fadhila Al-Farid ◽  
Jamaluddin Jamaluddin ◽  
Andi Sukainah

This study aims to determine the quality of jackfruit seed juice drinks with the addition of red ginger and to determine the level of acceptance of panelists on jackfruit seed juice drinks. Parameters observed included water content, protein content, carbohydrate content and organoleptic including color, taste, aroma and texture. This study used a Completely Randomized Design (CRD) consisting of one factor, namely the concentration of red ginger juice with four treatments, namely: 0%, 6%, 12%, 18%, each repeated three times. The results showed that jackfruit seed juice drink with the addition of 18% red ginger was the best treatment from the results of organoleptic tests. The proximate test results showed that the best treatment was 3.13% water content in 12% ginger treatment, 2.17% protein in 18% red ginger treatment and 95.61 carbohydrate in 18% red ginger treatment.


SIMBIOSA ◽  
2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Fenny Agustina ◽  
Agam Akhmad Syaukani ◽  
Fatimah Sahara

The study was conducted over two months, from March to April 2012. This study aims to analyze the influence of type of packaging materials for durability of chilli (Capsicum annum L.) during cold storage. The method used in this study is an experimental method using a completely randomized design (CRD), which consists of four types of packaging materials, namely of chilli are packed in plastic PE, PP, PS, PS + control film with a red pepper that is stored without packaging. The parameters analyzed are severe shrinkage and the organoleptic (texture, color, aroma and overall). From the test results obtained ANAVA Fhitung value of 203,869 is greater than the value Ftabel, both at the level of 5% for 3,480 and 5,990 at 1% level. For the organoleptic value of PS + plastic film gives the highest yield in each test the texture attributes of 6,678. 6,500 color, aroma and overall 6,122 and 6,722. PP plastic gives the lowest result in each test the texture attributes of 3,156, 3,556 color, aroma and overall 3,333 and 3,300, this indicates that there is a very significant effect between the type of packaging materials for durability chili during cold storage.


2021 ◽  
Vol 3 (3) ◽  
pp. 119-127
Author(s):  
Runa Matias Ruku ◽  
Alfred G.O Kase ◽  
Hartini R.L Solle

ABSTRACTEucheuma cottonii (E. cottonii) seaweed is a low level plant that has a high nutritional value. One of the ingredients that play a role in the formation of texture is carrageenan.Carrageenan is a type of hydrocolloid extracted from the seaweed of the red algae group (Rhodophyceae). The purpose of this study was to determine the quality of yields of Seaweed Carrageenan Extract (E. cottonii) obtained from Tablolong beach in the form of yield, ash content and fat content. The method used in this study is an experimental method with a completely randomized design (CRD) and for fat testing using quantitative descriptive methods. This study uses various concentrations of 0.1 N KOH, 0.2 N KOH, 0.3 N KOH and 0.4 N KOH. The results of the carrageenan yield test show that the addition of 0.1 N KOH concentration has a value of 56.10, KOH 0 , 2 N 62.16, KOH 0.3 N 72.73 and the best treatment was at P4 KOH 0.4 N 80.90. Carrageenan ash content test results showed that the addition of KOH concentration value obtained P1 KOH 0.1 N was 60.50, P2 KOH 0.2 N was 70.70, P3 KOH 0.3 N was 80.50, and P4 KOH 0.4 with a value of 90.50. The results of the carrageenan fat content of 0.1 KOH treatment has a fat content of 0, 2 while the treatment of KOH 0,2, KOH 0,3, KOH 0,4 have no fat content by showing the value (0). The results showed that the highest yield in the treatment of KOH concentration of 0.4 N with a value 80.95% and carrageenan ash content has the highest value in the treatment concentration of 0.4 N with a value of 90.50% while the fat content has a value of 0.2 in the treatment of KOH concentration of 0.1 N Keywords: Carrageenan, extract of E. cottonii seaweed.


AGRICA ◽  
2020 ◽  
Vol 5 (2) ◽  
pp. 142-152
Author(s):  
Jenny E. R. Markus ◽  
Yuliana Tandi Rubak ◽  
Handy Tomasoei

This research was conducted in the Seed Technology laboratory and Laboratory of Microbiology, Faculty of Agriculture, University of Nusa Cendana, Kupang, which lasts from September to December 2013 with aims to investigate the influence of substitution of wheat flour with flour suweg the physicochemical properties and organoleptic cake. This study used a completely randomized design (CRD) with 5 treatments, namely substitution suweg flour to wheat flour: 0%, 10%, 20%, 30% and 40%. The parameters analyzed were proximate, softness, porosity, degree of development, test organoleptic colour, flavour, aroma, and texture. Organoleptic data obtained from the 20 panellists and subsequently analyzed by Friedman test, while ANOVA test if there is a real effect then tested further by using DMRT. The results showed that the substitution of different flour suweg provides a very significant effect on water content, fat, ash content, protein, carbohydrate esandtenderness cake. Based on the results of the organoleptic test, cake with flour substitution suweg the colour, aroma and taste of a real significant show, panellists liked the cake substitution suweg 40%, while the texture, the panellists still like to substitution of 40% flour suweg, an assessment of the texture with the highest scores on substitution treatment 10% flour suweg. Organoleptic test and Friedman test results it can be concluded that the cake treatment received a positive response from the panellists was a cake with 40% flour suweg treatment with a total of 66 rankings for colour, aroma as a total ranking of 68, and for a sense of the total ranking of 74. Similarly, some a score on the cake substitution acceptance panellists ranged from 3,1 to 3,4 and the obtained cake was elected with 40% substitution treatment suweg flour.


2020 ◽  
Vol 5 (1) ◽  
pp. 30
Author(s):  
Rima Sulastri ◽  
Made Darawati ◽  
Ketut Swirya Jaya

Background : Utilization of Rice Snails (Pila ampullacea) in the Making of Nugget with the Addition of Vitamins and Minerals (Carrots and Beans) which Contain High Protein to Overcome Nutritional Problems of PEM (Protein Energy Lack) in Toddler Children. Objectives : To determine the effect of carrot and bean addition on organoleptic properties and acceptability of toddlers on the SITELCIS nugget (snail carrot snail). Research Methods: This study used an experimental method in the laboratory with a Completely Randomized Design (CRD) of 1 factor, namely the addition of Carrots and Beans. Performed 5 levels of treatment and repetition 3 times with the addition of different Carrots and Beans. Organoleptic Properties Test Using the hedonic test method. Research Results : Anova Statistical Test Results on Panelists (Tukey) Effect of Carrots and Beans on Flavor (p <0.05). While the parameters of color, aroma and texture have no effect or (p> 0.05). The most preferred treatment for the highest average score is the taste parameter in treatment t4 with the addition of carrots and beans 20%. Conclusion : Based on the results of the test results of the acceptance of the SITELCIS Nugget in toddlers aged 4-5 years at the level of treatment t4 with the addition of carrots and beans (20%). From the results of the acceptance of children under five with given per serving of nuggets, the intake of good intake (≥ 80%) as many as 22 people, while the receiving power is less (<80%), which is as many as 3 people.


2018 ◽  
Vol 3 (2) ◽  
pp. 65
Author(s):  
Dwiki Tri Satya Wahyan ◽  
Ristika Handarini ◽  
Dede Kardaya

Breast and thigh are the parts of duck carcass which are most commonly utilized and consumed by people.  These carcass parts consist of meat, skin, and bones.  This study was aimed at assessing the effects of the inclusion of torch ginger flower solution in commercial ration on live weight and percentages of carcass and its parts of male local ducks.  The study was conducted from June to August 2016 at Poultry Laboratory of Animal Science Study Program, Faculty of Agriculture, Djuanda University, Bogor.  Twenty-four male local ducks aged 2 weeks with average initial body weight of 450 ± 53.04 g were used.  A completely randomized design with 4 treatments and 3 replicates was used.  Treatments consisted of commercial ration + 0% torch ginger flower solution (R0), commercial ration + 2.5% torch ginger flower solution (R1), commercial ration + 5.0% torch ginger flower solution (R2), and commercial ration + 7.5% torch ginger flower solution (R3).  Measurements were taken on the percentages of whole breast, breast meat, breast bones, whole thigh, thigh meat, and thigh bones.  Data were subjected to an analysis of variance and a Duncan test.  Results showed that treatments gave significant effect (P<0.05) on the percentage of thigh meat.  The inclusion of torch ginger flower solution in ration did not negatively affect carcass parts of male local ducks.  The inclusion of 7.5% torch ginger flower solution in commercial ration increased the percentage of whole breast.  It was also found that the inclusion of 5.0 and 7.5% torch ginger flower solution in commercial ration increased the percentage of thigh meat.Key words: torch ginger flower solution, male local duck, carcass parts


2019 ◽  
Vol 2 (1) ◽  
pp. 18
Author(s):  
Yani Yani ◽  
Moh Nuh Ibrahim ◽  
Suwarjoyowirayatno Suwarjoyowirayatno

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh penambahan daging gurita terhadap nilai organoleptik, proksimat stik gurita. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari tiga perlakuan yaitu P1 (Daging gurita 35%), P2 (Daging gurita 45%), P3 (Daging gurita 55%). Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (p>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncam Multiple Range Test) pada taraf nyata 95%. Kandungan protein dan lemak diuji menggunakan metode AOAC dan kandungan karbohidrat di uji menggunakan metode nelson-smogiy. Hasil uji kandungan kadar lemak dan karbohidrat tertinggi terdapat pada perlakuan P1 dengan nilai 34,27% dan 7,81% dan Kandungan protein tertinggi pada perlakuan P3 dengan nilai 32,04%. Hasil penelitian ini menunjukan bahwa terdapat pengaruh nyata terhadap kandungan lemak dan karbohidrat serta tidak terdapat pengaruh nyata pada kandungan air dan protein pada stik gurita. Kata kunci: Daging gurita (Octopus cyanea), stik gurita, kandungan proksimat, uji organoleptik  AbstractThis study aims to determine the effect Octopus cyanea addition on organoleptic values and proximate content of octopus sticks. This research method used a completely randomized design (CRD) consisting of three treatments P1 (35% octopus meat), P2 (45% octopus meat), P3 (55% octopus meat). Observation data were analyzed using ANOVA (Analysis of Variance) at the level 95%, if there were significant differences (p>0,05) then further testing was carried out with DMRT (Duncan Multiple Range Test) at 95%. The content of protein and fat was tested by the AOAC method and the carbohydrate content was tested by the nelson-smogiy method. The highest test results for the content of  fat and carbohydrate were found in treatment P1 with a value of 34.27% and 7.81% and the highest protein content found in treatment P3 with a value of 32.04%. The results of this study indicated that there is a real effect on the content of fat and carbohydrates and there is no significant effect on the water content and protein of octopus sticks. Key words: Octopus cyanea, octopus stick, proximate content, organoleptic value


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