scholarly journals The effect of blue and red light on physiological and biochemical characteristics of wheat plants

2020 ◽  
Vol 27 ◽  
pp. 19-22
Author(s):  
I. V. Azizov ◽  
F. I. Gasymova ◽  
U. F. Ibragimova ◽  
K. R. Tagiyeva ◽  
A. B. Abdullayeva
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Chloride ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Blue Light ◽  
Ascorbate Peroxidase ◽  
Red Light ◽  
Wheat Variety ◽  
Soluble Carbohydrates ◽  
Physiological And Biochemical

Aim. The effect of blue and red light on the activity of catalase and ascorbate peroxidase, the content of proteins and soluble carbohydrates in the leaves of wheat varieties under the action of sodium chloride were investigated. Methods. The objects of research were bread wheat Saratovskaya-29 (Triticum aestivum L.) and durum wheat Barakatli-95 (Triticum durum Desf.). Plants were grown in laboratory conditions in an aqueous medium using a Knoop nutrient medium. Experimental plants were coated with transparent films transmitting light at wavelengths of 420-480 nm (blue light) and 640-680 nm (red light). During the growing season, samples of fully formed leaves were taken for physiological and biochemical studies every week at 11 a.m. Results. Under the action of sodium chloride, the accumulation of hydrogen peroxide in white light was higher than in blue and red light (table). Low catalase activity was also observed in blue and red light under the influence of sodium chloride in both varieties. In bread wheat variety Saratovskaya -29 level of hydrogen peroxide, the activity of catalase and ascorbate peroxidase enzymes was lower than that of the Barakatli-95 durum wheat variety. Conclusions. Blue light stimulated the synthesis of proteins, while red light stimulated the synthesis of carbohydrates. Blue and red light prevented the formation of H2O2 under the influence of NaCl. Keywords: blue light, red light, catalase, ascorbate peroxidase, proteins, carbohydrates.

scholarly journals Physiological and Biochemical Response to Fusarium culmorum Infection in Three Durum Wheat Genotypes at Seedling and Full Anthesis Stage

2021 ◽  
Vol 22 (14) ◽  
pp. 7433
Author(s):  
Jakub Pastuszak ◽  
Anna Szczerba ◽  
Michał Dziurka ◽  
Marta Hornyák ◽  
Przemysław Kopeć ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Fusarium Culmorum ◽  
Soluble Carbohydrates ◽  
Head Blight ◽  
Wheat Genotypes ◽  
Modern Breeding ◽  
Bound Phenolics ◽  
Physiological And Biochemical ◽  
Yield Decrease ◽  
Selection Of

Fusarium culmorum is a worldwide, soil-borne plant pathogen. It causes diseases of cereals, reduces their yield, and fills the grain with toxins. The main direction of modern breeding is to select wheat genotypes the most resistant to Fusarium diseases. This study uses seedlings and plants at the anthesis stage to analyze total soluble carbohydrates, total and cell-wall bound phenolics, chlorophyll content, antioxidant activity, hydrogen peroxide content, mycotoxin accumulation, visual symptoms of the disease, and Fusarium head blight index (FHBi). These results determine the resistance of three durum wheat accessions. We identify physiological or biochemical markers of durum wheat resistance to F. culmorum. Our results confirm correlations between FHBi and mycotoxin accumulation in the grain, which results in grain yield decrease. The degree of spike infection (FHBi) may indicate accumulation mainly of deoxynivalenol and nivalenol in the grain. High catalase activity in the infected leaves could be considered a biochemical marker of durum sensitivity to this fungus. These findings allowed us to formulate a strategy for rapid evaluation of the disease severity and the selection of plants with higher level, or resistance to F. culmorum infection.

Effect of previous crops on crown rot and yield of durum and bread wheat in northern NSW

2004 ◽  
Vol 55 (3) ◽  
pp. 321 ◽  
Author(s):  
J. A. Kirkegaard ◽  
S. Simpfendorfer ◽  
J. Holland ◽  
R. Bambach ◽  
K. J. Moore ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Bread Wheat ◽  
Triticum Turgidum ◽  
Wheat Variety ◽  
Bipolaris Sorokiniana ◽  
Triticum Aestivum L ◽  
Crown Rot ◽  
Residual Water ◽  
Brassica Napus L ◽  
Trichoderma Spp

The effect of previous crops (oilseed, legume, and cereal) on the incidence and severity of crown rot (Fusarium pseudograminearum, Fp) and yield of wheat was investigated in 3 field studies in northern New South Wales. The experiments were designed to compare the effectiveness of the Brassica break crops canola (Brassica napus L.) and mustard (B. juncea L.) with chickpea (Cicer arietinum L.) on reduction of Fp in subsequent wheat crops. Responses to previous broadleaf and cereal crops were investigated in Fp-tolerant bread wheat (Triticum aestivum L.) and Fp-susceptible durum wheat [Triticum turgidum L. ssp. durum (Dest.)]. In all experiments, broadleaf break crops increased the yield of Fp-susceptible durum wheat compared with durum after cereals (by 0.24–0.89 t/ha). The same response was observed for the Fp-tolerant wheat at 2 of the 3 sites (0.71 and 0.78 t/ha), with a lower yield (0.13 t/ha) after break crops than after cereals at one site during a drought. The yield of the Fp-susceptible durum wheat was generally higher after brassicas than after chickpea (yield advantage 0.27–0.58�t/ha), whereas there was no such difference in the tolerant wheat variety. In most cases, these yield responses to the previous crops were closely related to the severity of Fp infection. Overall yield of susceptible durum wheat was reduced by 1% for each 1% increase in Fp severity at harvest. Residual water and nitrogen (N) did not explain responses to previous crops, although common root rot (Bipolaris sorokiniana) may have contributed to some of the responses at the sites. There was little evidence that the lower disease and higher yield following brassicas compared with chickpea was related to suppression of Fp by biofumigation. More plausible explanations are that residual cereal residues decomposed more rapidly under dense Brassica canopies thus reducing Fp inoculum, that Fp severity was increased following chickpea due to higher soil N status, or that brassicas resulted in soil/residue biology that was less conducive to Fp inoculum survival. Evidence for the latter was provided by consistently higher levels of Trichoderma spp. isolated from wheat following brassicas compared with chickpea or cereals. Irrespective of the mechanisms involved, the results demonstrate that Brassica oilseeds provide an effective break crop for crown rot in northern NSW. Furthermore, brassicas may provide an excellent alternative rotation crop to chickpea for high value durum wheat due to an apparent capacity to more effectively reduce the severity of crown rot infection in subsequent crops.

scholarly journals Exogenous abscisic acid impacts the development of isolated immature endosperm in bread wheat

2021 ◽  
Author(s):  
Iwona Chłosta ◽  
Małgorzata Kozieradzka-Kiszkurno ◽  
Dagmara Kwolek ◽  
Izabela Marcińska ◽  
Apolonia Sieprawska ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Bread Wheat ◽  
Positive Impact ◽  
Wheat Variety ◽  
Culture Conditions ◽  
Soluble Carbohydrates ◽  
In Planta ◽  
Specific Storage ◽  
The Media

AbstractEndosperm in cereals such as wheat, is a part of the mature seeds and a valuable source of key substances for humans and animals. For this reason, the development of immature endosperm tissues in planta was the focus of this research. However, it is commonly known that tissue culture conditions can alter the developmental pathway of plant cells and can expose their potency. There is scarce information about research on isolated endosperm in wheat. The development of isolated immature endosperm in the winter bread wheat variety ‘Kobra’, depending on the media composition, is presented in this study. Abscisic acid (ABA) is a key plant growth regulator for proper seed development. The addition of exogenous ABA had a positive impact on the size and ultrastructural features in isolated endosperm, especially of the outer aleurone-like cells. Furthermore, the content of starch in the endosperm cultured on a medium with ABA did not significantly differ from that of caryopsis at the same age, in contrast to soluble carbohydrates. Fluorescein diacetate (FDA) staining and confocal microscopy observation confirmed the viability of the cells from the outer layers. The analysis of internucleosomal fragmentation of DNA in the explants suggests the induction of programmed cell death (PCD) and DNA degradation typical of necrosis. We concluded that the development of isolated immature endosperm in bread wheat depends on the composition of the media. Thus, it could be a model for in vitro studies of this specific storage tissue and its response to culture conditions in bread wheat.

scholarly journals The effect of varietal traits of durum wheat on the technological and baking qualities of flour when mixed with bread wheat

2021 ◽  
pp. 67-72
Author(s):  
E. N. Shabolkina ◽  
N. V. Anisimkina
Keyword(s):  
Durum Wheat ◽  
Bread Wheat ◽  
Wheat Flour ◽  
Wheat Variety ◽  
Rheological Parameters ◽  
Wheat Bread ◽  
Physical Parameters ◽  
Wheat Varieties ◽  
Mutual Compensation ◽  
Improvement Effect

The development of bakery industry is possible due to the use of such non-traditional raw materials as durum wheat. The purpose of the current study was to estimate the effect of varietal traits of durum wheat when mixed with bread wheat according to the results of rheological parameters of dough, technological and bakery estimation of flour. There have been studied technological indicators of grain, rheological and physical parameters of dough, general bakery estimation. There has been established that the high gas-forming ability of durum wheat allows it to be used (30%) as bread wheat improver during baking. However, the positive effect was present not in all years of the study. There has been estimated an improvement effect due to mutual compensation of the missing components and complementarity of the bread and durum wheat varieties. There was found that in 2008, 2010 there was practically no improvement effect when durum wheat flour was added to the mixture in a ratio of 30:70%. There was established that in 2015 the maximum bread volume of 930 cm3 and a good bakery estimation (flat surface, oval shape, golden brown crust, as well as fine thin-walled porosity with elastic light crumb) were obtained by adding bread wheat varieties to durum wheat varieties, which during the year of the study there was formed weak grain (dilute of dough was 110 u.f.; valorigraphic number was 46 u.v.). In 2020, the varieties used in the mixtures of both spring bread and durum wheat were of high quality, and bakery estimation gave excellent indicators both in the control (the variety ‘Tulaykovskaya 108’ with 1300 cm3) and in the mixtures with 1140–1255 cm3; the appearance of bread and crumb in almost all variants had an excellent mark. The largest volume of bread, 1255 cm3, was obtained when the durum wheat variety ‘Bezenchukskaya Niva’ was added to the mixture. Adding durum wheat flour to the mixture in an amount of 30:70% when baking bread reduced its staleness by 6.5% relative to the control (bread wheat); bread remains fresh for a long time with an elastic, quickly regenerated crumb.

Confirmation of chloride deficiency as the cause of leaf spotting in durum wheat grown in the Australian northern grains region

2015 ◽  
Vol 66 (2) ◽  
pp. 122 ◽  
Author(s):  
G. D. Schwenke ◽  
S. R. Simpfendorfer ◽  
B. C. Y. Collard
Keyword(s):  
North America ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Field Experiments ◽  
Wheat Variety ◽  
Yield Response ◽  
Wheat Varieties ◽  
Breeding Lines ◽  
Whole Plant ◽  
Delayed Flowering

During the 2007 winter cropping season in Australia, severe leaf-spotting (necrosis) symptoms resembling chloride (Cl–) deficiency found in North America were reported in the newly released durum wheat variety Jandaroi. Testing for bacterial, fungal and viral pathogens all proved negative. Four Australian durum and four Australian bread wheat varieties were grown, along with a North American variety of each, in a glasshouse experiment using a sterile sand–vermiculite mix and nutrient solutions containing 0 (nil), 0.5, 1.0 or 2.0 mm Cl–. When grown in the nil Cl– solution, all durum and some bread wheat varieties produced leaf-spotting symptoms the same as observed in the field. Nil Cl– also delayed flowering, reduced biomass, decreased grain size, and depressed grain yield in most durum and bread wheat varieties. In field experiments, additions of Cl– fertiliser as KCl at sowing provided no biomass or yield response from a range of wheat varieties, probably because the plants accessed sufficient Cl– from below 0.9 m depth in the soil. Chloride concentrations in whole-plant tissue sampled at head emergence suggested that unfertilised plants were borderline deficient in Cl– according to critical values established in North America. An in-crop foliar Cl– application experiment showed linear uptake of applied Cl–, as MgCl2, until the end of tillering. However, because leaf-spotting symptoms typically appear only after tillering, it is not possible to correct Cl– deficiency by adding Cl– fertiliser to the affected crop after symptoms appear. Managing Cl– in susceptible crops therefore needs to be preventative rather than curative. Among commercial varieties, Jandaroi was highly sensitive to low Cl–, Caparoi was moderately sensitive, and EGA Bellaroi was tolerant. Several elite durum breeding lines grown in 2010 showed considerably reduced leaf spotting compared with Jandaroi under low Cl– conditions, indicating potential for conventional breeding to reduce the potential impact of low Cl– soils on durum production in northern Australia.

Physicochemical and Dough-handling Characteristics of Indian Wheat and Triticale Cultivars

2010 ◽  
Vol 16 (5) ◽  
pp. 371-379 ◽  
Author(s):  
H.R. Naik ◽  
K.S. Sekhon ◽  
A. Abbas Wani
Keyword(s):  
Particle Size ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Wheat Variety ◽  
Compositional Analysis ◽  
Quality Parameter ◽  
Strong Correlations ◽  
Handling Characteristics ◽  
End Use ◽  
Wheat Flours

Four bread wheat (PBW-138, PBW-299, PBW-343 and PBW-373), two durum wheat (PDW-215 and PDW-233) and two triticale cultivars (TL-419 and TL-1210) were investigated for physicochemical, milling and dough-handling properties for predicting end-use quality. Physical properties of durum wheat (PDW-215) and bread wheat (PBW-138, PBW-299 and PBW-343) cultivars were better than other wheat, durum and triticale cultivars. The compositional analysis revealed nonsignificant differences between the different cultivars; however, starch observed significant variation for different varieties. Particle size distribution indicated that triticale flours showed lower particle size than wheat and durum wheat. Dough-handling studies revealed triticale flours to be the weakest, while bread wheat flours were observed to be intermediate between durum and triticale. Among all the varieties, the bread wheat (PBW-138) variety was observed to be best, followed by PDW-215 durum wheat variety. Strong correlations were observed between physicochemical and dough-handling parameters, which can be used as quality parameter for suitable end-use.

scholarly journals Genetic diversity of high and low molecular weight glutenin subunits in Saharan bread and durum wheats from Algerian oases

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 23-32 ◽  
Author(s):  
I. Bellil ◽  
M. Chekara Bouziani ◽  
D. Khelifi
Keyword(s):  
Genetic Diversity ◽  
Molecular Weight ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Allelic Variation ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Lmw Glutenin Subunits ◽  
Genotypic Identification ◽  
Diversity Studies

Saharan wheats have been studied particularly from a botanical viewpoint. Genotypic identification, classification and genetic diversity studies to date were essentially based on the morphology of the spike and grain. For this, the allelic variation at the glutenin loci was studied in a set of Saharan bread and durum wheats from Algerian oases where this crop has been traditionally cultivated. The high molecular weight and low molecular weight glutenin subunit composition of 40 Saharan bread and 30 durum wheats was determined by SDS-PAGE. In Saharan bread wheats 32 alleles at the six glutenin loci were detected, which in combination resulted in 36 different patterns including 17 for HMW and 23 for LMW glutenin subunits. For the Saharan durum wheats, 29 different alleles were identified for the five glutenin loci studied. Altogether, 29 glutenin patterns were detected, including 13 for HMW-GS and 20 for LMW-GS. Three new alleles were found in Saharan wheats, two in durum wheat at the Glu-B1 and Glu-B3 loci, and one in bread wheat at the Glu-B1 locus. The mean indices of genetic variation at the six loci in bread wheat and at the five loci in durum wheat were 0.59 and 0.63, respectively, showing that Saharan wheats were more diverse. This information could be useful to select Saharan varieties with improved quality and also as a source of genes to develop new lines when breeding for quality.

scholarly journals Phototropin 1 and dim-blue light modulate the red light de-etiolation response

2014 ◽  
Vol 9 (11) ◽  
pp. e976158
Author(s):  
Yihai Wang ◽  
Kevin M Folta
Keyword(s):  
Blue Light ◽  
Red Light

scholarly journals Development and validation of a method for quantification of common wheat, durum wheat, rye and barley by droplet digital PCR

2021 ◽  
Author(s):  
Christian Schulze ◽  
Anne-Catrin Geuthner ◽  
Dietrich Mäde
Keyword(s):  
Durum Wheat ◽  
Real Time ◽  
Bread Wheat ◽  
Common Wheat ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Food Fraud ◽  
Single Genome ◽  
Cereal Species

AbstractFood fraud is becoming a prominent topic in the food industry. Thus, valid methods for detecting potential adulterations are necessary to identify instances of food fraud in cereal products, a significant component of human diet. In this work, primer–probe systems for real-time PCR and droplet digital PCR (ddPCR) for the detection of these cereal species: bread wheat (together with spelt), durum wheat, rye and barley for real-time PCR and ddPCR were established, optimized and validated. In addition, it was projected to validate a molecular system for differentiation of bread wheat and spelt; however, attempts for molecular differentiation between common wheat and spelt based on the gene GAG56D failed because of the genetic variability of the molecular target. Primer–probe systems were further developed and optimized on the basis of alignments of DNA sequences, as well as already developed PCR systems. The specificity of each system was demonstrated on 10 (spelt), 11 (durum wheat and rye) and 12 (bread wheat) reference samples. Specificity of the barley system was already proved in previous work. The calculated limits of detection (LOD95%) were between 2.43 and 4.07 single genome copies in real-time PCR. Based on the “three droplet rule”, the LOD95% in ddPCR was calculated to be 9.07–13.26 single genome copies. The systems were tested in mixtures of flours (rye and common wheat) and of semolina (durum and common wheat). The methods proved to be robust with regard to the tested conditions in the ddPCR. The developed primer–probe systems for ddPCR proved to be effective in quantitatively detecting the investigated cereal species rye and common wheat in mixtures by taking into account the haploid genome weight and the degree of milling of a flour. This method can correctly detect proportions of 50%, 60% and 90% wholemeal rye flour in a mixture of wholemeal common wheat flour. Quantitative results depend on the DNA content, on ploidy of cereal species and are also influenced by comminution. Hence, the proportion of less processed rye is overestimated in higher processed bread wheat and adulteration of durum wheat by common wheat by 1–5% resulted in underestimation of common wheat.

scholarly journals Assessment of Morpho-Physiological and Biochemical Responses of Mercury-Stressed Trigonella foenum-gracum L. to Silver Nanoparticles and Sphingobacterium ginsenosidiumtans Applications

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1349
Author(s):  
Ahlam Khalofah ◽  
Mona Kilany ◽  
Hussein Migdadi
Keyword(s):  
Heavy Metals ◽  
Hydrogen Peroxide ◽  
Photosynthetic Pigments ◽  
Ag Nanoparticles ◽  
Thymus Vulgaris ◽  
Total Phenols ◽  
Biochemical Responses ◽  
Mercury Stress ◽  
Relative Water ◽  
Physiological And Biochemical

Heavy metals are primarily generated and deposited in the environment, causing phytotoxicity. This work evaluated fenugreek plants’ morpho-physiological and biochemical responses under mercury stress conditions toward Ag nanoparticles and Sphingobacterium ginsenosidiumtans applications. The fabrication of Ag nanoparticles by Thymus vulgaris was monitored and described by UV/Vis analysis, FTIR, and SEM. The effect of mercury on vegetative growth was determined by measuring the root and shoots length, the number and area of leaves, the relative water content, and the weight of the green and dried plants; appraisal of photosynthetic pigments, proline, hydrogen peroxide, and total phenols content were also performed. In addition, the manipulation of Ag nanoparticles, S. ginsenosidiumtans, and their combination were tested for mercury stress. Here, Ag nanoparticles were formed at 420 nm with a uniform cuboid form and size of 85 nm. Interestingly, the gradual suppression of vegetal growth and photosynthetic pigments by mercury, Ag nanoparticles, and S. ginsenosidiumtans were detected; however, carotenoids and anthocyanins were significantly increased. In addition, proline, hydrogen peroxide, and total phenols content were significantly increased because mercury and S. ginsenosidiumtans enhance this increase. Ag nanoparticles achieve higher levels by the combination. Thus, S. ginsenosidiumtans and Ag nanoparticles could have the plausible ability to relieve and combat mercury’s dangerous effects in fenugreek.

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