Phage strategies facilitate bacterial coexistence under environmental variability

Bacterial communities are often exposed to temporal variations in resource availability, which exceed bacterial generation times and thereby affect bacterial coexistence. Bacterial population dynamics are also shaped by bacteriophages, which are a main cause of bacterial mortality. Several strategies are proposed in the literature to describe infections by phages, such as “Killing the Winner”, “Piggyback the loser” (PtL) or “Piggyback the Winner” (PtW). The two temperate phage strategies PtL and PtW are defined by a change from lytic to lysogenic infection when the host density changes, from high to low or from low to high, respectively. To date, the occurrence of different phage strategies and their response to environmental variability is poorly understood. In our study, we developed a microbial trophic network model using ordinary differential equations (ODEs) and performed ‘in silico’ experiments. To model the switch from the lysogenic to the lytic cycle, we modified the lysis rate of infected bacteria and their growth was turned on or off using a density-dependent switching point. We addressed whether and how the different phage strategies facilitate bacteria coexistence competing for limiting resources. We also studied the impact of a fluctuating resource inflow to evaluate the response of the different phage strategies to environmental variability. Our results show that the viral shunt (i.e. nutrient release after bacterial lysis) leads to an enrichment of the system. This enrichment enables bacterial coexistence at lower resource concentrations. We were able to show that an established, purely lytic model leads to stable bacterial coexistence despite fluctuating resources. Both temperate phage models differ in their coexistence patterns. The model of PtW yields stable bacterial coexistence at a limited range of resource supply and is most sensitive to resource fluctuations. Interestingly, the purely lytic phage strategy and PtW both result in stable bacteria coexistence at oligotrophic conditions. The PtL model facilitates stable bacterial coexistence over a large range of stable and fluctuating resource inflow. An increase in bacterial growth rate results in a higher resilience to resource variability for the PtL and the lytic infection model. We propose that both temperate phage strategies represent different mechanisms of phages coping with environmental variability. Our study demonstrates how phage strategies can maintain bacterial coexistence in constant and fluctuating environments.

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Baicalin Represses Type Three Secretion System of Pseudomonas aeruginosa through PQS System

Molecules ◽

10.3390/molecules26061497 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1497

Author(s):

Pansong Zhang ◽

Qiao Guo ◽

Zhihua Wei ◽

Qin Yang ◽

Zisheng Guo ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Virulence Factors ◽

Mammalian Cells ◽

Secretion System ◽

Chinese Herbs ◽

Infection Model ◽

Lung Pathology ◽

Promising Alternative ◽

The Impact

Therapeutics that target the virulence of pathogens rather than their viability offer a promising alternative for treating infectious diseases and circumventing antibiotic resistance. In this study, we searched for anti-virulence compounds against Pseudomonas aeruginosa from Chinese herbs and investigated baicalin from Scutellariae radix as such an active anti-virulence compound. The effect of baicalin on a range of important virulence factors in P. aeruginosa was assessed using luxCDABE-based reporters and by phenotypical assays. The molecular mechanism of the virulence inhibition by baicalin was investigated using genetic approaches. The impact of baicalin on P. aeruginosa pathogenicity was evaluated by both in vitro assays and in vivo animal models. The results show that baicalin diminished a plenty of important virulence factors in P. aeruginosa, including the Type III secretion system (T3SS). Baicalin treatment reduced the cellular toxicity of P. aeruginosa on the mammalian cells and attenuated in vivo pathogenicity in a Drosophila melanogaster infection model. In a rat pulmonary infection model, baicalin significantly reduced the severity of lung pathology and accelerated lung bacterial clearance. The PqsR of the Pseudomonas quinolone signal (PQS) system was found to be required for baicalin’s impact on T3SS. These findings indicate that baicalin is a promising therapeutic candidate for treating P. aeruginosa infections.

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The impact of quarantine on Covid-19 infections

Epidemiologic Methods ◽

10.1515/em-2020-0038 ◽

2021 ◽

Vol 10 (s1) ◽

Author(s):

Pablo Marshall

Keyword(s):

Health System ◽

Infection Rate ◽

District Level ◽

Infection Model ◽

Social Distancing ◽

Proposed Model ◽

Lower Infection ◽

The Impact ◽

Lower Infection Rate ◽

Over Time

Abstract Objectives: Coronavirushas had profound effects on people’s lives and the economy of many countries, generating controversy between the need to establish quarantines and other social distancing measures to protect people’s health and the need to reactivate the economy. This study proposes and applies a modification of the SIR infection model to describe the evolution of coronavirus infections and to measure the effect of quarantine on the number of people infected. Methods: Two hypotheses, not necessarily mutually exclusive, are proposed for the impact of quarantines. According to the first hypothesis, quarantine reduces the infection rate, delaying new infections over time without modifying the total number of people infected at the end of the wave. The second hypothesis establishes that quarantine reduces the population infected in the wave. The two hypotheses are tested with data for a sample of 10 districts in Santiago, Chile. Results: The results of applying the methodology show that the proposed model describes well the evolution of infections at the district level. The data shows evidence in favor of the first hypothesis, quarantine reduces the infection rate; and not in favor of the second hypothesis, that quarantine reduces the population infected. Districts of higher socio-economic levels have a lower infection rate, and quarantine is more effective. Conclusions: Quarantine, in most districts, does not reduce the total number of people infected in the wave; it only reduces the rate at which they are infected. The reduction in the infection rate avoids peaks that may collapse the health system.

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Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

Microorganisms ◽

10.3390/microorganisms9040762 ◽

2021 ◽

Vol 9 (4) ◽

pp. 762

Author(s):

Lucia Henrici De Angelis ◽

Noemi Poerio ◽

Vincenzo Di Pilato ◽

Federica De Santis ◽

Alberto Antonelli ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Parental Strain ◽

Bacterial Infections ◽

Capsular Polysaccharide ◽

Galleria Mellonella ◽

Bacterial Pathogen ◽

Phage Resistance ◽

Infection Model ◽

Lytic Phage ◽

Susceptible Host

Phage therapy is now reconsidered with interest in the treatment of bacterial infections. A major piece of information for this application is the definition of the molecular targets exploited by phages to infect bacteria. Here, the genetic basis of resistance to the lytic phage φBO1E by its susceptible host Klebsiella pneumoniae KKBO-1 has been investigated. KKBO-1 phage-resistant mutants were obtained by infection at high multiplicity. One mutant, designated BO-FR-1, was selected for subsequent experiments, including virulence assessment in a Galleria mellonella infection model and characterization by whole-genome sequencing. Infection with BO-FR-1 was associated with a significantly lower mortality when compared to that of the parental strain. The BO-FR-1 genome differed from KKBO-1 by a single nonsense mutation into the wbaP gene, which encodes a glycosyltransferase involved in the first step of the biosynthesis of the capsular polysaccharide (CPS). Phage susceptibility was restored when BO-FR-1 was complemented with the constitutive wbaP gene. Our results demonstrated that φBO1E infects KKBO-1 targeting the bacterial CPS. Interestingly, BO-FR-1 was less virulent than the parental strain, suggesting that in the context of the interplay among phage, bacterial pathogen and host, the emergence of phage resistance may be beneficial for the host.

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Friend or Foe? Impacts of Dietary Xylans, Xylooligosaccharides, and Xylanases on Intestinal Health and Growth Performance of Monogastric Animals

Animals ◽

10.3390/ani11030609 ◽

2021 ◽

Vol 11 (3) ◽

pp. 609

Author(s):

Jonathan T. Baker ◽

Marcos E. Duarte ◽

Debora M. Holanda ◽

Sung Woo Kim

Keyword(s):

Crop Residues ◽

Structural Difference ◽

Nutrient Release ◽

Food Product ◽

Degree Of Polymerization ◽

Poultry Production ◽

Intestinal Health ◽

Sustainable Food ◽

And Performance ◽

The Impact

This paper discusses the structural difference and role of xylan, procedures involved in the production of xylooligosaccharides (XOS), and their implementation into animal feeds. Xylan is non-starch polysaccharides that share a β-(1-4)-linked xylopyranose backbone as a common feature. Due to the myriad of residues that can be substituted on the polymers within the xylan family, more anti-nutritional factors are associated with certain types of xylan than others. XOS are sugar oligomers extracted from xylan-containing lignocellulosic materials, such as crop residues, wood, and herbaceous biomass, that possess prebiotic effects. XOS can also be produced in the intestine of monogastric animals to some extent when exogenous enzymes, such as xylanase, are added to the feed. Xylanase supplementation is a common practice within both swine and poultry production to reduce intestinal viscosity and improve digestive utilization of nutrients. The efficacy of xylanase supplementation varies widely due a number of factors, one of which being the presence of xylanase inhibitors present in common feedstuffs. The use of prebiotics in animal feeding is gaining popularity as producers look to accelerate growth rate, enhance intestinal health, and improve other production parameters in an attempt to provide a safe and sustainable food product. Available research on the impact of xylan, XOS, as well as xylanase on the growth and health of swine and poultry, is also summarized. The response to xylanase supplementation in swine and poultry feeds is highly variable and whether the benefits are a result of nutrient release from NSP, reduction in digesta viscosity, production of short chain xylooligosaccharides or a combination of these is still in question. XOS supplementation seems to benefit both swine and poultry at various stages of production, as well as varying levels of XOS purity and degree of polymerization; however, further research is needed to elucidate the ideal dosage, purity, and degree of polymerization needed to confer benefits on intestinal health and performance in each respective species.

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Spatial and Temporal Distribution of Cattle Dung and Nutrient Cycling in Integrated Crop–Livestock Systems

Agronomy ◽

10.3390/agronomy10050672 ◽

2020 ◽

Vol 10 (5) ◽

pp. 672

Author(s):

Sandoval Carpinelli ◽

Adriel Ferreira da Fonseca ◽

Pedro Henrique Weirich Neto ◽

Santos Henrique Brant Dias ◽

Laíse da Silveira Pontes

Keyword(s):

Nutrient Cycling ◽

Temporal Distribution ◽

Nutrient Release ◽

Cattle Dung ◽

Total N ◽

Light Reduction ◽

Residue Decomposition ◽

Thiessen Polygon ◽

The Impact ◽

Livestock Systems

Residue decomposition from cattle dung is crucial in the nutrient cycling process in Integrated Crop–Livestock Systems (ICLS). It also involves the impact of the presence of trees exerted on excreta distribution, as well as nutrient cycling. The objectives of this research included (i) mapping the distribution of cattle dung in two ICLS, i.e., with and without trees, CLT and CL, respectively, and (ii) quantification of dry matter decomposition and nutrient release (nitrogen—N, phosphorus—P, potassium—K, and sulphur—S) from cattle dung in both systems. The cattle dung excluded boxes were set out from July 2018 to October 2018 (pasture phase), and retrieved after 1, 7, 14, 21, 28, 56 and 84 days (during the grazing period). The initial concentrations of N (~19 g kg−1), P (~9 g kg−1), K (~16 g kg−1), and S (~8 g kg−1) in the cattle dung showed no differences. The total N, P, K and S released from the cattle dung residues were less in the CLT system (2.2 kg ha−1 of N; 0.7 kg ha−1 of P; 2.2 kg ha−1 of K and 0.6 kg ha−1 of S), compared to the CL (4.2 kg ha−1 of N; 1.4 kg ha−1 of P; 3.6 kg ha−1 of K and 1.1 kg ha−1 of S). Lesser quantities of cattle dung were observed in the CLT (1810) compared to the CL (2652), caused by the lower stocking rate, on average, in this system (721 in the CL vs. 393 kg ha−1 in the CLT) because of the reduced amount of pasture in the CLT systems (−41%), probably due to light reduction (−42%). The density of the excreta was determined using the Thiessen polygon area. The CL system revealed a higher concentration of faeces at locations near the water points, gate and fences. The CLT affects the spatial distribution of the dung, causing uniformity. Therefore, these results strengthen the need to understand the nutrient release patterns from cattle dung to progress fertilisation management.

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The Effects of Aβ1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2

International Journal of Molecular Sciences ◽

10.3390/ijms22158226 ◽

2021 ◽

Vol 22 (15) ◽

pp. 8226

Author(s):

John Tsu-An Hsu ◽

Chih-Feng Tien ◽

Guann-Yi Yu ◽

Santai Shen ◽

Yi-Hsuan Lee ◽

...

Keyword(s):

Angiotensin Converting Enzyme ◽

Viral Entry ◽

Negative Impact ◽

Spike Protein ◽

Infection Model ◽

Converting Enzyme ◽

Viral Receptor ◽

Angiotensin Converting Enzyme 2 ◽

People With Dementia ◽

The Impact

Increasing evidence suggests that elderly people with dementia are vulnerable to the development of severe coronavirus disease 2019 (COVID-19). In Alzheimer’s disease (AD), the major form of dementia, β-amyloid (Aβ) levels in the blood are increased; however, the impact of elevated Aβ levels on the progression of COVID-19 remains largely unknown. Here, our findings demonstrate that Aβ1-42, but not Aβ1-40, bound to various viral proteins with a preferentially high affinity for the spike protein S1 subunit (S1) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the viral receptor, angiotensin-converting enzyme 2 (ACE2). These bindings were mainly through the C-terminal residues of Aβ1-42. Furthermore, Aβ1-42 strengthened the binding of the S1 of SARS-CoV-2 to ACE2 and increased the viral entry and production of IL-6 in a SARS-CoV-2 pseudovirus infection model. Intriguingly, data from a surrogate mouse model with intravenous inoculation of Aβ1-42 show that the clearance of Aβ1-42 in the blood was dampened in the presence of the extracellular domain of the spike protein trimers of SARS-CoV-2, whose effects can be prevented by a novel anti-Aβ antibody. In conclusion, these findings suggest that the binding of Aβ1-42 to the S1 of SARS-CoV-2 and ACE2 may have a negative impact on the course and severity of SARS-CoV-2 infection. Further investigations are warranted to elucidate the underlying mechanisms and examine whether reducing the level of Aβ1-42 in the blood is beneficial to the fight against COVID-19 and AD.

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Parasite infection and host dynamics in a naturally fluctuating rodent population

Canadian Journal of Zoology ◽

10.1139/z2012-083 ◽

2012 ◽

Vol 90 (9) ◽

pp. 1149-1160 ◽

Cited By ~ 17

Author(s):

J.C. Winternitz ◽

M.J. Yabsley ◽

S.M. Altizer

Keyword(s):

Population Density ◽

Experimental Studies ◽

Host Density ◽

Positive Association ◽

Population Cycles ◽

Host Population ◽

Reproductive Status ◽

Vole Cycles ◽

Host Infection ◽

The Impact

Parasites can both influence and be affected by host population dynamics, and a growing number of case studies support a role for parasites in causing or amplifying host population cycles. In this study, we examined individual and population predictors of gastrointestinal parasitism on wild cyclic montane voles ( Microtus montanus (Peale, 1848)) to determine if evidence was consistent with theory implicating parasites in population cycles. We sampled three sites in central Colorado for the duration of a multiannual cycle and recorded the prevalence and intensity of directly transmitted Eimeria Schneider, 1875 and indirectly transmitted cestodes from a total of 267 voles. We found significant associations between host infection status, individual traits (sex, age, and reproductive status) and population variables (site, trapping period, and population density), including a positive association between host density and cestode prevalence, and a negative association between host density and Eimeria prevalence. Both cestode and Eimeria intensity correlated positively with host age, reproductive status, and population density, but neither parasite was associated with poorer host condition. Our findings suggest that parasites are common in this natural host, but determining their potential to influence montane vole cycles requires future experimental studies and long-term monitoring to determine the fitness consequences of infection and the impact of parasite removal on host dynamics.

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In VivoEfficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.04324-14 ◽

2015 ◽

Vol 59 (4) ◽

pp. 2113-2121 ◽

Cited By ~ 9

Author(s):

U. Malik ◽

O. N. Silva ◽

I. C. M. Fensterseifer ◽

L. Y. Chan ◽

R. J. Clark ◽

...

Keyword(s):

Antimicrobial Agents ◽

Cyclic Peptide ◽

Sequence Similarity ◽

Infection Model ◽

Content Type ◽

Wide Range ◽

Inhibitory Activities ◽

The Impact

ABSTRACTStaphylococcus aureusis a virulent pathogen that is responsible for a wide range of superficial and invasive infections. Its resistance to existing antimicrobial drugs is a global problem, and the development of novel antimicrobial agents is crucial. Antimicrobial peptides from natural resources offer potential as new treatments against staphylococcal infections. In the current study, we have examined the antimicrobial properties of peptides isolated from anuran skin secretions and cyclized synthetic analogues of these peptides. The structures of the peptides were elucidated by nuclear magnetic resonance (NMR) spectroscopy, revealing high structural and sequence similarity with each other and with sunflower trypsin inhibitor 1 (SFTI-1). SFTI-1 is an ultrastable cyclic peptide isolated from sunflower seeds that has subnanomolar trypsin inhibitory activity, and this scaffold offers pharmaceutically relevant characteristics. The five anuran peptides were nonhemolytic and noncytotoxic and had trypsin inhibitory activities similar to that of SFTI-1. They demonstrated weakin vitroinhibitory activities againstS. aureus, but several had strong antibacterial activities againstS. aureusin anin vivomurine wound infection model. pYR, an immunomodulatory peptide fromRana sevosa, was the most potent, with complete bacterial clearance at 3 mg · kg−1. Cyclization of the peptides improved their stability but was associated with a concomitant decrease in antimicrobial activity. In summary, these anuran peptides are promising as novel therapeutic agents for treating infections from a clinically resistant pathogen.

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Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Scientific Reports ◽

10.1038/s41598-021-90208-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Helal F. Hetta ◽

Israa M. S. Al-Kadmy ◽

Saba Saadoon Khazaal ◽

Suhad Abbas ◽

Ahmed Suhail ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Acinetobacter Baumannii ◽

Biofilm Formation ◽

Microtiter Plate ◽

Diffusion Method ◽

Resistance Pattern ◽

Infection Model ◽

The Impact

AbstractWe aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

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Absorption coefficient of urban aerosol in Nanjing, west Yangtze River Delta, China

Atmospheric Chemistry and Physics ◽

10.5194/acp-15-13633-2015 ◽

2015 ◽

Vol 15 (23) ◽

pp. 13633-13646 ◽

Cited By ~ 15

Author(s):

B. L. Zhuang ◽

T. J. Wang ◽

J. Liu ◽

Y. Ma ◽

C. Q. Yin ◽

...

Keyword(s):

Absorption Coefficient ◽

Urban Area ◽

Yangtze River ◽

Temporal Variations ◽

Yangtze River Delta ◽

River Delta ◽

Near Surface ◽

Absorbing Aerosols ◽

The Impact ◽

532 Nm

Abstract. Absorbing aerosols can significantly modulate short-wave solar radiation in the atmosphere, affecting regional and global climate. The aerosol absorption coefficient (AAC) is an indicator that assesses the impact of absorbing aerosols on radiative forcing. In this study, the near-surface AAC and absorption Ångström exponent (AAE) in the urban area of Nanjing, China, are characterized on the basis of measurements in 2012 and 2013 using the seven-channel Aethalometer (model AE-31, Magee Scientific, USA). The AAC is estimated with direct and indirect corrections, which result in consistent temporal variations and magnitudes of AAC at 532 nm. The mean AAC at 532 nm is about 43.23 ± 28.13 M m−1 in the urban area of Nanjing, which is much lower than that in Pearl River Delta and the same as in rural areas (Lin'an) in Yangtze River Delta. The AAC in the urban area of Nanjing shows strong seasonality (diurnal variations); it is high in cold seasons (at rush hour) and low in summer (in the afternoon). It also shows synoptic and quasi-2-week cycles in response to weather systems. Its frequency distribution follows a typical log-normal pattern. The 532 nm AAC ranging from 15 to 65 M m−1 dominates, accounting for more than 72 % of the total data samples in the entire study period. Frequent high pollution episodes, such as those observed in June 2012 and in winter 2013, greatly enhanced AAC and altered its temporal variations and frequency distributions. These episodes are mostly due to local emissions and regional pollution. Air masses flowing from northern China to Nanjing can sometimes be highly polluted and lead to high AAC at the site. AAE at 660/470 nm from the Schmid correction (Schmid et al., 2006) is about 1.56, which might be more reasonable than from the Weingartner correction (Weingartner et al., 2003). Low AAEs mainly occur in summer, likely due to high relative humidity (RH) in the season. AAC increases with increasing AAE at a fixed aerosol loading. The RH–AAC relationship is more complex. Overall, AAC peaks at RH values of around 40 % (1.3 < AAE < 1.6), 65 % (AAE < 1.3 and AAE > 1.6), and 80 % (1.3 < AAE < 1.6).

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