scholarly journals Polyphenols composition, antioxidant and antimicrobial properties of Pinus sylvestris L. shoots extracts depending on different drying methods

2020 ◽  
pp. 229 ◽  
Author(s):  
Marcin Dziedziński ◽  
Joanna Kobus-Cisowska ◽  
Daria Szymanowska- Powałowska ◽  
Kinga Stuper- Szablewska ◽  
Marlena Baranowska
Keyword(s):  
Pinus Sylvestris ◽  
Bioactive Compounds ◽  
Raw Materials ◽  
Antimicrobial Properties ◽  
Raw Material ◽  
Drying Methods ◽  
Active Components ◽  
Antioxidant Power ◽  
Freeze Dried ◽  
Ferric Reducing Antioxidant Power

Scots pine (Pinus sylvestris L.) shoots have been increasingly commonly used as functional food or its component, the shoots contain various active components, unknown from other raw materials. The objective of the study was to assess the influence of the drying method on the content of bioactive compounds and antioxidative and antimicrobial activity of pine shoots. It was demonstrated that freeze drying (PSL), vacuum drying (PSP) and natural drying (PSN) have significant impact on the physicochemical properties, content of bioactive compounds and antioxidative activity of the prepared ethanol-water extracts. The content of the studied compounds varied significantly in the tested shoots. In spectrophotometric testing the highest total flavonoid content was demonstrated in the PSP sample, at 5.51 mg quercetin/g dw. On the other hand, the reducing capacity was as follows: PSN>PSP>PSL in the range from 13.4 to 5.73 mg gallic acid/g dw. However in assay conducted using HPLC methods the highest content of polyphenols characterized extract from freeze-dried raw material (9151.15 µg/g), followed by vacuum-dried (8264.57 µg/g), and the lowest content of phenolic compounds was found in convection-dried shoots (7621.76 µg/g). The studied extracts demonstrated antioxidative properties, both in ferric reducing antioxidant power assay (FRAP) as well as in free radical quenching measurement (DPPH). All of the studied extracts demonstrated antimicrobial and fungicidal properties, and they were particularly efficient in the case of gram-negative bacteria.

scholarly journals Comparative Examination of Antioxidant Capacity and Fingerprinting of Unfractionated Extracts from Different Plant Parts of Quinoa (Chenopodium quinoa) Grown under Greenhouse Conditions

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 238
Author(s):  
Dayana Buitrago ◽  
Ivon Buitrago-Villanueva ◽  
Ricardo Barbosa-Cornelio ◽  
Ericsson Coy-Barrera
Keyword(s):  
Antioxidant Capacity ◽  
Plant Material ◽  
Radical Scavenging ◽  
Chenopodium Quinoa ◽  
Total Content ◽  
Drying Methods ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Freeze Dried ◽  
Ferric Reducing Antioxidant Power

Integrated surveys of metabolic profiles and antioxidant capacity from Chenopodium quinoa have been limited and have particularly focused on an examination of seeds and leaves. According to this, the main aim of the present study was to address an evaluation of the antioxidant activity of crude ethanolic extracts from different plant parts (leaves, stems, roots, flowers, and seeds) harvested at different times during growth and processed by two distinct drying methods: Air-drying and freeze-drying. In order to characterize the resulting extracts, the total content of phenolics (TPC) and flavonoids (TFC) was then measured through the Folin–Ciocalteu method, while antioxidant capacity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical scavenging and ferric-reducing antioxidant power (FRAP) methods. Parallel to this evaluation, extracts were profiled by LC-DAD-ESI-MS. Data analysis was supported by statistics. Most of the extracts obtained from freeze-dried samples showed higher TPC values ranging from 6.02 to 43.47 milligram of gallic acid equivalents per gram of plant material and a TFC between 1.30 and 12.26 milligram of quercetin equivalents per gram of plant material. After statistical analysis, a low correlation between TPC and TFC values was observed regarding antioxidant capacity from DPPH and FRAP measurements of both drying methods. A multivariate analysis showed that antioxidant components and antioxidant capacity in C. quinoa changed during growth and between plant parts and drying methods. These changes need to be taken into consideration when comparing the production/accumulation of beneficial bioactive compounds in this pseudocereal.

scholarly journals Characteristics and consumer acceptance level of Sargassum hystrix nori

2021 ◽  
Vol 919 (1) ◽  
pp. 012030
Author(s):  
S T Firmansyah ◽  
A Husni
Keyword(s):  
Initial Conditions ◽  
Raw Materials ◽  
Proximate Analysis ◽  
Consumer Acceptance ◽  
Raw Material ◽  
Antidiabetic Activity ◽  
Total Phenol Content ◽  
Antioxidant Power ◽  
Acceptance Level ◽  
Ferric Reducing Antioxidant Power

Abstract Nori is processed seaweed that is quite popular, including in Indonesia. The purpose of this study was to determine the characteristics and consumer acceptance level of nori from Sargassum hystrix seaweed. S. hystrix (in wet and/or dry form) was soaked in 0.01% NaOH, then blended into a slurry. S. hystrix slurry was molded in size 15x15 cm and dried in an oven (70°C, 3 hours). The analysis included thickness, hardness, proximate analysis, antioxidant activity with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing antioxidant power (FRAP), antidiabetic activity, and hedonic tests. The results of this study indicate that the composition of the initial conditions of raw materials has a significant effect (P<0.05) on the characteristics and consumer acceptance level of S. hystrix nori. The best treatment was obtained on nori made from the initial raw material in wet form with the characteristics of a thickness of 0.34 mm, hardness of 335.21 gf, water content of 14.47%, ash content of 21.22%, protein content of 7.51%, fat content of 9.64%, total phenol content of 21.01 mg GAE/g, DPPH inhibition activity 62.77%, FRAP value 133.50 M/g, (-glucosidase inhibitory activity 43.81%, and consumer acceptance level of appearance 2.66, aroma 2.41, texture 3.06, flavour 3.26, and color 2.98.

scholarly journals Identifying bioactive phytochemicals in spent coffee grounds for cosmetic application through global metabolite analysis

2017 ◽  
Author(s):  
◽  
Jihyun Park
Keyword(s):  
Bioactive Compounds ◽  
Raw Materials ◽  
Costa Rican ◽  
Raw Material ◽  
Spent Coffee Grounds ◽  
Metabolite Analysis ◽  
Coffee Grounds ◽  
Chemical Profiles ◽  
Anti Oxidant ◽  
Anti Inflammatory

Annually, more than 6 million tons of spent coffee grounds (SCG) are generated worldwide. The present study explores the possible use of spent coffee grounds as the raw materials for cosmetics industry. The main objective of this project are to investigate the chemical profiles and identify the bioactive compounds for cosmetics application through global metabolite analysis. The compounds extracted from SCG of Ethiopia coffee (Yirgacheffe), Costa Rican coffee (Tarrazu) and Hawaiian coffee (Kona) were analyzed by ultra-high pressure liquid chromatography coupled with mass spectrometry (UPLC-MS). The ion chromatograms were submitted to XCMS platform operated by Center for Metabolomics at the Scripps Research Institute. The peak detection, peak grouping, spectra extraction, and retention alignment were processed by XCMS. The spectra were annotated and the compounds were identified and categorized by integration with METLIN, the world's largest metabolite database. Multivariate and univariate statistical analysis including PCA and cloud-plot were performed by XCMS to compare the chemical profiles between the three coffee cultivars. These analyses indicated that each cultivar showed a specific cluster. Over 200 compounds related to anti-oxidant, anti-inflammatory, anti-tyrosinase and anti-tumor for skin care application were identified by XCMS. Therefore, the presence of bioactive compounds in SCG makes it a potential source of raw material for cosmetic application (e.g., anti-oxidant, anti-inflammatory, skin-whiting, and anti-aging).

scholarly journals Viability of lactic acid bacteria in different components of Ogi with anti diarrhoeagenic E. coli activities

2019 ◽  
Vol 3 (6) ◽  
pp. 206-213
Author(s):  
Roseline Eleojo Kwasi ◽  
Iyanuoluwa Gladys Aremu ◽  
Qudus Olamide Dosunmu ◽  
Funmilola A. Ayeni
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antimicrobial Properties ◽  
Culture Method ◽  
Antimicrobial Activities ◽  
Drying Methods ◽  
Bacterial Strains ◽  
E Coli ◽  
Log Reduction ◽  
Freeze Dried

Background: Ogi constitutes a rich source of lactic acid bacteria (LAB) with associated health benefits to humans through antimicrobial activities. However, the high viability of LAB in Ogi and its supernatant (Omidun) is essential. Aims: This study was carried out to assess the viability of LAB in various forms of modified and natural Ogi and the antimicrobial properties of Omidun against diarrhoeagenic E coli. Methods and Material: The viability of LAB was assessed in fermented Ogi slurry and Omidun for one month and also freeze-dried Ogi with and without added bacterial strains for two months. A further 10 days viability study of modified Omidun, refrigerated Omidun, and normal Ogi was performed. The antimicrobial effects of modified Omidun against five selected strains of diarrhoeagenic E. coli (DEC) were evaluated by the co-culture method. Results: Both drying methods significantly affected carotenoids and phenolic compounds. The Ogi slurry had viable LAB only for 10 days after which, there was a succession of fungi and yeast. Omidun showed 2 log10cfu/ml reduction of LAB count each week and the freeze-dried Ogi showed progressive reduction in viability. Refrigerated Omidun has little viable LAB, while higher viability was seen in modified Omidun (≥2 log cfu/ml) than normal Omidun. Modified Omidun intervention led to 2-4 log reduction in diarrhoeagenic E. coli strains and total inactivation of shigella-toxin producing E. coli H66D strain in co-culture. Conclusions: The consumption of Ogi should be within 10 days of milling using modified Omidun. There are practical potentials of consumption of Omidun in destroying E. coli strains implicated in diarrhea. Keywords: Ogi, Omidun, lactic acid bacteria, diarrhoeagenic Escherichia coli strains, Viability.

scholarly journals Evaluation of the Biocompatibility of Cleaned Bovine Tendon in a Heterotopic Implantation Model

2021 ◽  
pp. 327-334
Author(s):  
Maksim Gurin ◽  
◽  
Aleksey Venediktov ◽  
Yuliya Glumskova ◽  
Kristina Korneeva ◽  
...  
Keyword(s):  
Raw Materials ◽  
Tween 80 ◽  
Biologically Active ◽  
Raw Material ◽  
Optimal Size ◽  
Active Components ◽  
Physical Treatment ◽  
Urgent Task ◽  
Ligament Prosthesis ◽  
Implantation Model

Damage to the tendon-ligamentous apparatus places serious limitations on a personʼs physical activity. Injuries are especially common in physically healthy people leading an active lifestyle, such as athletes. To treat such injuries in orthopaedics and traumatology, autoplastic operations are performed or prostheses made of synthetic or biological materials are installed. The known treatment methods, in spite of their effectiveness, have a number of serious drawbacks, which often limit their use. Therefore, the search for new approaches and materials for plastic ligaments is an urgent task. Today, biotissue prostheses are accumulating advantages over their synthetic counterparts. The most promising raw material for biological ligament prostheses, due to its availability in the required quantity and optimal size, is the flexor and extensor calf tendons. This paper aimed to develop a method for treating xenogenic tendon to manufacture ligament prostheses and assessing its biocompatibility in a heterotopic implantation model. To manufacture a ligament prosthesis, the raw material was subjected to mechanical cleaning and chemical-physical treatment, as well as treatment with supercritical carbon dioxide fluid with the addition of the nonionic surfactant Tween 80, which together contributed to effective decellularization and removal of other biologically active components, while maintaining the physical and mechanical parameters and natural fiberarchitectonics of native raw materials. The biocompatible properties of ligament prosthesis specimens made from the flexor and extensor calf tendons using this method were evaluated in a model of heterotopic implantation into the subcutaneous adipose tissue of rats. The results obtained confirm the promising use of this material, treated according to the proposed method, in clinical practice.

Effect of Drying on the Bioactive Compounds and Antioxidant Activity of Rubus lambertianus

2018 ◽  
Vol 14 (2) ◽  
Author(s):  
Jun Yu ◽  
Zijian Shangguan ◽  
Xingju Yang ◽  
Dan Sun ◽  
Baoqing Zhu ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Drying Methods ◽  
Protective Effects ◽  
Red Raspberry ◽  
Hot Air Drying ◽  
Hot Air ◽  
Freeze Dried ◽  
Total Anthocyanins

AbstractDifferent drying methods, including hot air drying (HAD), freeze drying (FD), spray drying and vacuum drying (VD), were investigated to determine their influence on the chromatic coordinates, phenolics, anthocyanins and antioxidant activities of dried red raspberry (Rubus lambertianus). Pelargonidin-3-O-glucoside and catechin were found to be the main anthocyanin and non-anthocyanin phenolics, respectively, in fresh red raspberry. The most effective method for controlling browning was FD. The highest protective effects against bioactive compounds were observed in freeze-dried powders, when measuring the total anthocyanins, the scavenging of DPPH (1,1-diphenyl-2-picrylhydrazyl) and hydroxyl radicals and the inhibition effects on lard oxidation. HAD was effective for the preservation of total phenolics, while VD was useful for protecting catechin and procyanidin B1.

scholarly journals Studies on Proximate Composition, Mineral and Total Phenolic Content of Yogurt Bites Enriched with Different Plant Raw Material

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 301
Author(s):  
Jurgita Kulaitienė ◽  
Nijolė Vaitkevičienė ◽  
Dovilė Levickienė
Keyword(s):  
Proximate Composition ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Raw Materials ◽  
Sugar Content ◽  
Raw Material ◽  
Total Phenolic ◽  
Mulberry Leaves ◽  
Freeze Dried ◽  
Plant Raw Material

Yogurt products are consumed by millions of people every day. Consumers’ priority for ready-to-eat yogurt snacks enriched with various plant raw materials have increased each year. Therefore, the aim of this study was to prepare freeze-dried yoghurt bites with the addition of powders of beetroot, mulberry leaves, nettle leaves and rosehip fruit and to investigate these raw materials’ influence on the proximate composition, mineral and total phenolic content. The moisture, protein, fat, carbohydrate and sugar content of the yogurt bites were established using standard methods: mineral composition—using an inductively coupled plasma mass spectrometer (ICP-MS); total phenolic content—by the spectrophotometric method. The results demonstrated that the addition of different raw material powders to the formulation of yogurt bites had no significant influence on carbohydrate, protein and total fat amounts. However, the incorporation of powders of beetroot, mulberry leaves, nettle leaves and rosehip fruit in yogurt bites allowed a significant increase of the amounts of all investigated minerals and total phenolic content of the manufactured bites. Among all investigated yogurt bites, the highest amounts of K, P, Mg, Fe and Zn were determined for yogurt bites enriched with nettle leaves. In conclusion, the enrichment of yogurt bites with freeze-dried plant raw material powders can increase amounts of selected minerals and total phenolic content.

scholarly journals Bioactive Compounds from Norway Spruce Bark: Comparison Among Sustainable Extraction Techniques for Potential Food Applications

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 524 ◽  
Author(s):  
Spinelli ◽  
Costa ◽  
Conte ◽  
Porta ◽  
Padalino ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Norway Spruce ◽  
Bioactive Compounds ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Industry Waste ◽  
Spruce Bark ◽  
Assisted Extraction ◽  
Ferric Reducing Antioxidant Power ◽  
Food Applications

Picea abies (L.) Karst, (Norway spruce) bark, generally considered as wood industry waste, could potentially be used as a valuable source of antioxidants for food applications. In this study, supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), and ultrasound-assisted extraction (UAE) were carried out in order to recover bioactive compounds from bark of Norway spruce. Obtained results show that PLE with ethanol as solvent was the most effective method for extracting total flavonoid compounds (21.14 ± 1.42 mg quercetin g−1 sample) and consequently exerted the highest antioxidant activity measured by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (257.11 ± 13.31 mg Trolox g−1 sample). On the other hand, UAE extract contained the maximum phenolic concentration (54.97 ± 2.00 mg gallic acid g−1 sample) and the most interesting antioxidant activity measured by the ferric-reducing antioxidant power (580.25 ± 25.18 µmol FeSO4 g−1 sample). Additionally, PLE and UAE have demonstrated great efficiency in the extraction of trans-resveratrol, quantified by HPLC (0.19 and 0.29 mg trans-RSV g−1 sample, respectively).

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Annona muricata By-Products and Pulp

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 904 ◽  
Author(s):  
Gabriela Aguilar-Hernández ◽  
María García-Magaña ◽  
María Vivar-Vera ◽  
Sonia Sáyago-Ayerdi ◽  
Jorge Sánchez-Burgos ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Raw Materials ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Total Polyphenols ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
By Products

Ultrasound-assisted extraction (UAE) is widely used; however, the efficiency of extraction depends on the raw materials. Therefore, optimization of UAE must be investigated for each type of plant material. By-products from soursop fruit have not been studied as a source of bioactive compounds. In this work, the optimization of UAE conditions (extraction time (5, 10, and 15 min), pulse cycle (0.4, 0.7, and 1 s), and sonication amplitude (40%, 70%, and 100%)) for the extraction of phenolic compounds (soluble, hydrolyzable, condensed tannins, and total polyphenols) from soursop by-products (seed, peel, and columella) and pulp was evaluated using response surface methodology. The optimal conditions for UAE to obtain the highest total polyphenol content from by-products and pulp was dependent on the raw material. Peel resulted in the highest content of total polyphenols (187.32 mg/g dry matter [DM]) followed by columella (164.14 mg/g DM), seed (36.15 mg/g DM), and pulp (33.24 mg/g DM). The yield of polyphenolic content from peel and columella obtained with UAE was higher (32–37%) than conventional extraction for 2 h under stirring (14–16%). The contents of gallic acid (0.36–15.86 µg/g DM), coumaric acid (0.07–1.37 µg/g DM), and chlorogenic acid (9.18–32.67 µg/g DM) in the different parts of the fruit were higher in the extracts obtained by UAE compared with a conventional extraction method (0.08–0.61, 0.05–0.08, 3.15–13.08 µg/g DM, respectively), although it was dependent on the raw materials. Soursop by-products can be functionally important if they are used to extract bioactive compounds by UAE; a technology with high potential for commercial extraction on a large scale.

scholarly journals Antioxidant and Hepatoprotective Effect of Aqueous Extract of Germinated and Fermented Mung Bean on Ethanol-Mediated Liver Damage

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Norlaily Mohd Ali ◽  
Hamidah Mohd Yusof ◽  
Kamariah Long ◽  
Swee Keong Yeap ◽  
Wan Yong Ho ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mung Bean ◽  
Serum Markers ◽  
Hepatoprotective Effect ◽  
Aqueous Extracts ◽  
Antioxidant Power ◽  
Freeze Dried ◽  
Ferric Reducing Antioxidant Power ◽  
Hepatoprotective Effects

Mung bean is a hepatoprotective agent in dietary supplements. Fermentation and germination processes are well recognized to enhance the nutritional values especially the concentration of active compounds such as amino acids and GABA of various foods. In this study, antioxidant and hepatoprotective effects of freeze-dried mung bean and amino-acid- and GABA-enriched germinated and fermented mung bean aqueous extracts were compared. Liver superoxide dismutase (SOD), malondialdehyde (MDA), ferric reducing antioxidant power (FRAP), nitric oxide (NO) levels, and serum biochemical profile such as aspartate transaminase (AST), alanine transaminase (ALT), triglycerides (TG), and cholesterol and histopathological changes were examined for the antioxidant and hepatoprotective effects of these treatments. Germinated and fermented mung bean have recorded an increase of 27.9 and 7.3 times of GABA and 8.7 and 13.2 times of amino acid improvement, respectively, as compared to normal mung bean. Besides, improvement of antioxidant levels, serum markers, and NO level associated with better histopathological evaluation indicated that these extracts could promote effective recovery from hepatocyte damage. These results suggested that freeze-dried, germinated, and fermented mung bean aqueous extracts enriched with amino acids and GABA possessed better hepatoprotective effect as compared to normal mung bean.

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