scholarly journals BLOOD MORPHOLOGICAL STATUS OF BLACK PIED SEED BULLSWHEN USING MINERAL SUPPLEMENT BASED ON MARAL ANTLER PRESS-CAKE

2021 ◽  
pp. 64-70
Author(s):  
A.I. Afanasyeva ◽  
◽  
V.A. Sarychev ◽  
Keyword(s):  
Press Cake ◽  
Individual Characteristics ◽  
Biologically Active ◽  
Control Group ◽  
Growth Promoters ◽  
Mineral Supplement ◽  
Animal Body ◽  
Altai Region ◽  
Morphological Status ◽  
Mean Cell Hemoglobin

The usage period of seed-bulls, their sexual activity, the amount and quality of semen obtained from them depend both on the individual characteristics of the animal organ-ism and numerous environmental factors. The diet should contain all the necessary organic substances: easily digest-ible carbohydrates (starch and sugar), lipids, minerals and vitamins. The lack or deficiency of macro-and trace ele-ments in the diet and inadequate ratio of individual mineral elements disrupts the processes of nutrient intake into the animal body, thereby contributing to the disruption of meta-bolic processes. However, the mineral composition of plant forages depends largely on soil and climatic conditions, production and storage techniques. The average trace element deficiency in energy-balanced diets is 30-50%, and that necessitates using mineral supplements in animal diets. The nature of changes occurring in an animal body when feeding new forages, feed supplements, biological growth promoters and other products should and may be controlled through the main blood values. The research goal was to study the blood morphological composition of Black Pied seed bulls when using a mineral supplement based on maral antler press-cake. The experimental stud-ies were carried out in the breeding company AO “Plempredpriyatiye Barnaulskoye”, the Altai Region, in 2021, in the control and trial groups of comparable Black Pied seed bulls used on the farms of the Altai Region. Along with the basic diet, the seed bulls of the trial group received the mineral supplement based on maral antler press-cake; the daily dose -15.0 g per head, for 30 days with 10 days’ interval aftereach application. The supple-ment was mixed with compound feed before feeding. The mineral supplement was made at the Federal Altai Scien-tific Center of Agro-Biotechnologiesafter fermentation and autoclaving of maral antler press-cake; a significant amount of biologically active substances was found in ant-ler press-cake. The morphological status of the blood of seed bulls when using the mineral supplement made from maral antlers was characterized by a higher counts of erythrocytes, hemoglobin and leukocytes by 8.8%, 8.31% and 3.6%, higher values of hematocrit volume, mean cell hemoglobin (MCH), mean corpuscular volume (MCV), and mean cell hemoglobin concentration (MCHC) by 9.78%, 0.3%, 1.8% and 1.4%, respectively, as compared to those blood indices of the control group animals; that was indica-tive of its hematopoietic effect.

scholarly journals BLOOD BIOCHEMICAL INDICES OF SEED BULLS WHEN USING MINERAL SUPPLEMENT BASED ON MARAL ANTLER PRESS-CAKE

2021 ◽  
pp. 44-51
Author(s):  
A.I. Afanasyeva ◽  
◽  
V.A. Sarychev ◽  
S.N. Chebakov ◽  
◽  
...  
Keyword(s):  
Press Cake ◽  
Good Health ◽  
Biologically Active ◽  
Control Group ◽  
Trial Group ◽  
Mineral Supplement ◽  
Genetic Characteristics ◽  
Blood Biochemical ◽  
Biochemical Indices ◽  
Blood Biochemical Indices

The seed bulls used for artificial insemination should be in good health, have outstanding genetic characteristics, have high sexual activity, and the biological products ob-tained from them should have high quality indices. One of the possible methods of increasing semen production is the introduction of dietary supplements into the diet. The intro-duction of new feed and biologically active supplements into the diet may cause both positive and negative changes in metabolic processes. Monitoring the biochemical status of blood allows detecting not only metabolic disorders in highly productive animals but also a lack of vitamins, mac-ro-and microelements. The research goal was to study the effect of a natural biostimulant (mineral supplement) made from maral antler press-cake on the blood biochemical indices ofBlack Pied seed bulls in the breeding company AO “Plempredpriyatiye Barnaulskoye”. Along with the basic diet, the seed bulls of the trial group received the natural biostimulant (mineral supplement) based on maral antler press-cake; the daily dose -15.0 g per head, for 30 days with 10 days’ interval after each application. The supple-ment was thoroughly mixed with compound feed before feeding. The mineral supplement is of powder form and contains a complex of vitamins, minerals, amino acids and other biologically active substances. The use of a mineral supplement increased the amount of total protein and al-bumin by 7.15% and 18.3%, respectively. The blood glu-cose level in the seed bulls of the trial group was higher by 12% and cholesterol by 17.1% than in the blood of the con-trol group animals. Cholesterol of the seed bulls may be used as a structural material or a precursor in the synthesis of sex steroid hormones. The level of total calcium, alkaline reserve at the end of the experiment increased in the seed bulls when using the mineral supplement made from maral antler press-cake in the diet by 4.8% and 8.1%, respective-ly, as compared to the control group.

scholarly journals Resistance of Dairy Cows During the Use of New Production Fodder

2021 ◽  
Author(s):  
Roman Kolesnikov ◽  
Vitaly Morozov ◽  
Anna Matserushka ◽  
Galina Talalay ◽  
Margarita Kolesnokova
Keyword(s):  
Dry Matter ◽  
Biologically Active ◽  
Control Group ◽  
Purchase Price ◽  
New Production ◽  
Mineral Supplement ◽  
Hydroponic System ◽  
Green Fodder ◽  
Experimental Group ◽  
Active Substances

This paper examines the development of a fundamentally new automated hydroponic system for growing green fodder, which creates a solid fodder base capable to satisfy the need of livestock for nutrients throughout the year with low material costs. The new hydroponic green fodder produced according to the developed technology contained the required nutrients that contribute to the digestion of proteins, fats and carbohydrates into easily digestible organic components (amino acids, fatty acids and simple sugars) which are simpler in their structure. Moreover, it completely replaced compound feed, a mineral supplement in the diet of milking cows, and reduced the amount of fodder needed. In dry matter, compared to fodder barley, the content of protein (128.93%) and fat (196.73%) were higher, and biologically active substances were also formed. The new fodder used the nutrients of the diet more efficiently, increased milk production and reduced fodder costs. When hydroponic green fodder from barley was introduced into the diet of cows with 100% replacement of the compound fodder and 50% replacement of grain mass, milk yield in the experimental group exceeded the yield in the control group by 6%. An additional 549.5 kg was obtained from each cow for lactation, and 549.5 × 20 = 10990 kg was obtained from the entire experimental group. The annual income from feeding green fodder was 329710 rubles at the purchase price of milk of 30 rubles / kg (10990 × 30), and the daily efficiency of compound fodder was (80.6 × 20) = 1612 rubles; for the period of lactation it was 491660 rubles. Keywords: technology, new fodder, productivity, milk quality, resistance

scholarly journals Productivity of pigs when feeding celluloseamylolytic additive

2020 ◽  
Vol 22 (92) ◽  
pp. 76-80 ◽  
Author(s):  
V. Y. Novakovska
Keyword(s):  
Live Weight ◽  
Barley Grain ◽  
Feed Additives ◽  
Neutral Detergent Fiber ◽  
Biologically Active ◽  
Feed Additive ◽  
Control Group ◽  
Mineral Supplement ◽  
Amylolytic Enzyme ◽  
Experimental Group

The use of different feed processing methods using exogenous feed additives to improve the digestibility of plant feed ingredients in pig feeding has been studied for decades. In the research laboratory of the Institute of Forage and Agriculture of Podillya NAAS, compositions of enzymes with cellulase and amylase activity were studied. They are able to effectively break down non-starch polysaccharides in cereals, increase digestibility and absorption of feed nutrients. The purpose of this work was to study the zootechnical efficiency of the use of biologically active in the composition of feed pigs for fattening. In the presented material, the influence of feeding cellulose amylolytic enzyme additive on the productive qualities of pigs for fattening is highlighted. The cellulose amylolytic additive contained α-amylase with an activity of 9342 IU/h and cellulase with an activity of 540 IU/h. Studies in pigs were performed in two stages: the equalization period (15 days) and the main (71 days). For feeding pigs, a feed mixture was used, consisting of feed grown directly on the farm – barley, wheat, soybeans. The diet of the control group consisted of 63 % barley grain, 27.5 % wheat grain, 9 % soybean meal 0.5 % mineral supplement. Pigs of the experimental group were fed cellulose amylolytic feed additive at the rate of 5 g of cellulase and 1 g of amylase per 1 kg of feed. Feeding supplements determines the intensity of the process of digestion of proteins, fats and carbohydrates. Cellulose-amylolytic additive affects the absorption of carbohydrates in the diet, providing an increase in the digestibility of the control and experimental groups, respectively, neutral-detergent fiber (38.2 and 46.7 %), acid-detergent fiber (18.7 and 36.5 %), the amount easily hydrolyzed carbohydrates (85.4 and 90.2 %), hemicellulose (65.7 and 61.5 %), cellulose (13.2 and 21.4 %). The decision on the feasibility of including cellulose-amylolytic additive in the diet can be confirmed on the basis of its use during the scientific and economic experiment. In the control group, the average daily increase in live weight was 725.07 g, and in the experimental group by 19.7 % more at the level of 902.53 g. It was also noted a positive effect on the formation of body biomass, development of individual organs and their systems.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

scholarly journals Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Fariba Heshmati Afshar ◽  
Masumeh Zadehkamand ◽  
Zahra Rezaei ◽  
Abbas Delazar ◽  
Vahideh Tarhriz ◽  
...  
Keyword(s):  
Cell Line ◽  
Proliferative Activity ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Flavonoid Glycosides ◽  
Control Group ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Cytotoxicity Activity ◽  
Methanolic Extracts

Abstract Background Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by 1H and 13C NMR. Results The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. Conclusion Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.

scholarly journals Effect of dietary copper sources on performance, gastric ghrelin-RNA expression, and growth hormone concentrations in serum in piglets

2019 ◽  
Vol 97 (10) ◽  
pp. 4242-4247 ◽  
Author(s):  
Ricardo Gonzalez-Esquerra ◽  
Raquel B Araujo ◽  
Douglas Haese ◽  
Joao L Kill ◽  
Anderson F Cunha ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Copper Chloride ◽  
Block Design ◽  
Control Diet ◽  
Phase 1 ◽  
Serum Levels ◽  
Control Group ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Serum Growth Hormone

Abstract Two performance studies were conducted to investigate the effects of 3 different sources of Cu on production parameters of piglets. A total of 256 piglets weaned at 24 ± 2 d were randomly allocated into 4 treatments with 10 or 8 replicates per treatment of 4 or 3 piglets per pen in Exp. 1 and 2, respectively. The experimental period was divided into 3 feeding phases: Phase 1 (24 to 35 d), Phase 2 (36 to 49 d), and Phase 3 (50 to 70 d). Treatments included a Control group (fed 10 mg/kg of Cu from CuSO4), a group fed 160 mg/kg of either CuSO4 (CuSO4-160) or tri-basic copper chloride (TBCC), and a group fed Cu methionine hydroxy analogue chelated (Cu-MHAC) at 150, 80, and 50 mg/kg in Phases 1, 2, and 3, respectively. The methionine value of Cu-MHAC was accounted during diet formulation to achieve the same levels of methionine across treatments. Phases 1 and 2 diets contained 2,200 and 1,500 ppm of ZnO, respectively; and antibiotics were used as growth promoters. Performance parameters were analyzed as completely randomized block design, in which each experiment was considered as a block. In trial 2, blood serum and mucosal samples, from the fundic region of the stomach, were collected from 1 piglet per replicate at day 70 and tested for serum growth hormone levels (GH) and ghrelin mRNA expression, respectively. The contrast between Cu-MHAC vs. CuSO4-160 + TBCC showed that piglets fed Cu-MHAC exhibited better feed conversion ratio (FCR) in all feeding phases compared with feeding inorganic Cu (P < 0.05). Overall, feeding Cu-MHAC improved body weight (BW), BW gain, feed intake (FI), and FCR vs. Control diet fed piglets; yet, it improved BW and FCR vs. TBCC fed piglets, and improved BW, BW gain, and FI vs. CuSO4-160 fed piglets (P < 0.05). Feeding TBCC promoted similar performance than feeding CuSO4-160, regardless of age (P > 0.05). Both ghrelin expression and growth hormone serum levels were significantly increased by feeding Cu-MHAC vs. Control diet fed animals (P < 0.01). Feeding CuSO4-160 upregulated ghrelin expression vs. Control (P < 0.01) while GH serum levels and ghrelin expression did no change by feeding TBCC compared with Control diet fed animals (P > 0.05). It was concluded that feeding Cu-MHAC at the levels tested herein can improve growth performance of piglets beyond feeding 160 ppm of either CuSO4 or TBCC, which may be partially explained by the increased expression of ghrelin and GH serum levels.

A NEW APPROACH FOR INVESTIGATING HYPOTHALAMO-PITUITARY-GONADAL AND ADRENAL FEEDBACK CONTROL MECHANISMS: ACTIVE IMMUNIZATION WITH STEROIDS

1974 ◽  
Vol 76 (3) ◽  
pp. 556-569 ◽  
Author(s):  
E. Nieschlag ◽  
K. H. Usadel ◽  
H. K. Kley ◽  
U. Schwedes ◽  
K. Schöffling ◽  
...  
Keyword(s):  
Feedback Control ◽  
Leydig Cells ◽  
Positive Response ◽  
Active Immunization ◽  
Biologically Active ◽  
Control Group ◽  
Control Mechanisms ◽  
New Approach ◽  
Biological Neutralization ◽  
Group Serum

ABSTRACT A new method for the investigation of hypothalamo-pituitary-gonadal and adrenal feedback control mechanisms based on the biological neutralization of gonadal and adrenal steroids by active immunization is proposed. The regulatory influence of a given steroid in the feedback control is proved when reduction of the free, biologically active fraction of this steroid caused by antibody binding induces a positive response of the pituitary, thus effecting gonadal or adrenal hypertrophy and hyperfunction. The advantages and limitations of the new model are demonstrated by the effects of active immunization of rabbits with cortisol (F), aldosterone (Aldo), dehydroepiandrosterone (DHA), androstenedione (Δ4-A), testosterone (T), 5α-dihydrotestosterone (5α-DHT), 5β-DHT and oestradiol (E2). In the immunized animals and in a control group serum concentrations of total corticosteroids (TC), DHA, T, Δ4-A, E1, E2, LH and FSH, the percentage of binding of steroids in serum and the specificity of the antisera are determined. The testes are evaluated by histometry and the nuclear volume of the adrenocortical and Leydig cells is measured.

scholarly journals Variations in situational risk factors for fractures of the distal forearm, hip, and vertebrae in older women

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wen-Yu Yu ◽  
Hei-Fen Hwang ◽  
Mau-Roung Lin
Keyword(s):  
Risk Factors ◽  
Soft Tissue ◽  
Older Women ◽  
Individual Characteristics ◽  
Situational Factors ◽  
Control Group ◽  
Distal Forearm ◽  
Forearm Fractures ◽  
Protective Response ◽  
Distal Forearm Fractures

Abstract Background Situational factors during a fall among three common types of fractures of the distal forearm, hip, and vertebrae among older women in Taiwan were investigated. Methods In 2016 ~ 2017, study participants were identified from those aged ≥65 years who visited emergency departments due to a fall in two university-affiliated hospitals in Taipei. In addition to individual characteristics, situational factors during the fall (location, activity, change of center of mass, fall mode, fall direction, initiating a protective response, and being hit) were collected. A sample of 203 distal-forearm fractures, 189 vertebral fractures, and 375 hip fractures was recruited, while 717 women with a soft-tissue injury were used as a control group. The identification of situational risk factors for each type of fracture was validated by using those who sustained one of the other two types of fracture as a control group. Results After adjusting for age and other individual characteristics, compared to soft-tissue injuries, distal-forearm fractures were significantly more likely to occur with slips (odds ratio [OR] = 11.0; 95% confidence interval [CI] = 4.76 ~ 25.4), trips (OR = 3.40; 95% CI = 1.42 ~ 8.17), step-downs (OR = 4.95; 95% CI = 2.15 ~ 11.4), and from sideways falls (OR = 1.73; 95% CI = 1.12 ~ 2.67) and significantly less likely to occur indoors (OR = 0.62; 95% CI = 0.42 ~ 0.90) or from backwards falls (OR = 0.62; 95% CI = 0.41 ~ 0.95). Hip fractures were significantly more likely to occur with step-downs (OR = 1.76; 95% CI = 1.13 ~ 2.75) and from backwards (OR = 3.16; 95% CI = 2.15 ~ 4.64) or sideways falls (OR = 5.56; 95% CI = 3.67 ~ 8.41) and significantly less likely when hitting an object (OR = 0.26; 95% CI = 0.13 ~ 0.52) or initiating a protective response (OR = 0.58; 95% CI = 0.36 ~ 0.93). Vertebral fractures were significantly more likely to occur with slips (OR = 2.42; 95% CI = 1.30 ~ 4.50), step-downs (OR = 2.53; 95% CI = 1.43 ~ 4.48), and backwards falls (OR = 2.15; 95% CI = 1.39 ~ 3.32). Similar results were found in the validation analyses. Conclusions Large variations in situational risk factors for the three types of fracture in older women existed. A combination of individual and situational risk factors may display a more-comprehensive risk profile for the three types of fracture, and an intervention that adds training programs on safe landing strategies and effective compensatory reactions may be valuable in preventing serious injuries due to a fall.

THE INFLUENCE OF SORPTION-DETOXIFYING MEANS ON CUMULATION OF 90SR IN BONE TISSUE OF WHITE RATES WITH COMBINED INTAKE OF 137CS, CD AND PB

2018 ◽  
Vol 1 (3) ◽  
pp. 129-133
Author(s):  
L. L. Zakharova ◽  
◽  
G. A. Zhorov ◽  
V. N. Obryvin ◽  
◽  
...  
Keyword(s):  
Bone Tissue ◽  
Chemical Nature ◽  
Mechanisms Of Action ◽  
The Body ◽  
Biologically Active ◽  
Laboratory Studies ◽  
Animal Body ◽  
White Rats ◽  
Combined Use ◽  
Active Substances

The article presents the results of laboratory studies of the effect of sorption-detoxifying means on the accumulation of 90Sr in the body of white rats. The efficiency of a number of selective and polyfunctional sorbents, detoxicants and other biologically active substances and the developed on their basis sorption-detoxifying complexes as means of efferent therapy and detoxification of the animal body at the combined intake of xenobiotics of radiation and chemical nature was evaluated. It was found that 90Sr cumulation in bones (at the level of 53,3–60,8%) was reduced to the greatest extent with the combined use of substances of different mechanisms of action and origin. The effectiveness of separate use of detoxifying drugs did not exceed 29,1%.

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