Nitric oxide mitigates salt stress effects of pepper seedlings by altering nutrient uptake, enzyme activity and osmolyte accumulation

Background: Salinity is one of the environmental stress factors that restrict the crop production by endangering agricultural areas. Nitric oxide (NO) protects plants from damage caused by oxidative stress conditions in various biological ways. Methods: In this greenhouse investigation during 2018, pea plants were irrigated with three levels of NaCl (0, 50 and 100 mM) solutions. NO solutions were prepared with three different doses (0, 75 and 100 µM SNP). These solutions were applied to the seeds before sowing and then to the leaves of the pea cultivars. The study was conducted to analyze the impact of NO on growth, malondialdehyde (MDA), hydrogen peroxide (H2O2), antioxidant enzyme activity and nutrient uptake in two pea cultivars under salinity conditions. Result: Salinity reduced fresh-dry weight, relative water content (RWC), and chlorophyll a and b content of pea. However, NO enhanced these parameters under salt stress. Salinity increased tissue electrical conductance (TEC), H2O2 and MDA content, which were decreased by combined application of NaCl and NO. Salinity caused an increase in antioxidant enzyme activity in pea and NO made a significant improvement in their activities under salinity conditions. Salinity treatments decreased the ratio of K+/Na+ and Ca2+/Na+ in both cultivars, and application of NO elevated them as compared to the control under salt stress. In conclude, exogenous NO treatment could help pea to tolerate salinity stress by increasing the chlorophyll content and regulating antioxidant enzyme activity and nutrient uptake.

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Nitric oxide improved salt stress tolerance by osmolyte accumulation and activation of antioxidant defense system in seedling of B. juncea (L.) Czern

Vegetos- An International Journal of Plant Research ◽

10.1007/s42535-019-00071-y ◽

2019 ◽

Vol 32 (4) ◽

pp. 583-592 ◽

Cited By ~ 4

Author(s):

Khushboo Khator ◽

Gyan Singh Shekhawat

Keyword(s):

Nitric Oxide ◽

Salt Stress ◽

Stress Tolerance ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System ◽

Salt Stress Tolerance ◽

Osmolyte Accumulation

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Effect of nitric oxide on Sertoli cell microtubule of piglets

Chinese Journal of Agricultural Biotechnology ◽

10.1017/s1479236209990064 ◽

2009 ◽

Vol 6 (3) ◽

pp. 257-263 ◽

Cited By ~ 1

Author(s):

Yang Li ◽

Wang Xian-zhong ◽

Yang Meng-bo ◽

Zhang Jia-hua

Keyword(s):

Nitric Oxide ◽

Enzyme Activity ◽

Protein Kinase ◽

Cell Viability ◽

Sodium Nitroprusside ◽

Sertoli Cell ◽

Sertoli Cells ◽

High Concentration ◽

Treatment Results ◽

Anti Oxidant

AbstractTo illustrate the effect of nitric oxide (NO) on the microtubules of Sertoli cells (SC), SCs of piglets were treated with sodium nitroprusside (SNP). Changes in cell viability, anti-oxidant activity, enzyme activity and p38 mutagen-activated protein kinase (p38MAPK) activation were detected. The results were as follows. A low concentration of NO can keep SC microtubule and cell viability normal, and a high concentration of NO could increase p38MAPK activation, decrease anti-oxidant activity and transferrin secretion, and destroy the structure and distribution of the microtubules. The results suggest that SNP treatment results in an increase in NO in SCs and decreased cell anti-oxidant activity. The high concentration of NO destroys cell microtubules by activating p38MAPK.

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Ethylene Supplementation Combined with Split Application of Nitrogen and Sulfur Protects Salt-Inhibited Photosynthesis through Optimization of Proline Metabolism and Antioxidant System in Mustard (Brassica juncea L.)

Plants ◽

10.3390/plants10071303 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1303

Author(s):

Badar Jahan ◽

Noushina Iqbal ◽

Mehar Fatma ◽

Zebus Sehar ◽

Asim Masood ◽

...

Keyword(s):

Salt Stress ◽

Brassica Juncea ◽

Antioxidant System ◽

Photosynthetic Efficiency ◽

Growth Potential ◽

Proline Metabolism ◽

Sowing Time ◽

Stress Effects ◽

Nutrient Assimilation ◽

S Application

In the present study, the potential of ethylene as ethephon (an ethylene source) was investigated individually and in combination with split doses of nitrogen (N) and sulfur (S) soil treatments for removal of the damaging effects of salt stress (100 mM NaCl) in mustard (Brassica juncea L.). Plants were grown with 50 mg N plus 50 mg S kg−1 soil at sowing time and an equivalent dose at 20 days after sowing [N50 + S50]0d and 20d. Ethephon at 200 μL L‒1 was applied to combined split doses of N and S with or without NaCl. Plants subjected to NaCl showed a decrease in growth and photosynthetic characteristics as well as N and S assimilation, whereas proline metabolism and antioxidants increased. The application of ethephon to plants grown with split N and S doses significantly enhanced photosynthetic efficiency by increasing the assimilation of N and S, improving the concentration of proline and induction of the antioxidant system with or without NaCl. The regulation of ethylene and/or split forms of N and S application may be potential tools for not just overcoming salt stress effects in this species and in related Brassicaceae but also enhancing their photosynthesis and growth potential through increased nutrient assimilation.

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Impact of Exogenous Application of Salicylic Acid on Growth, Water Status And Antioxidant Enzyme Activity of Strawberry Plants (Fragaria vesca L.) Under Salt Stress Conditions

Gesunde Pflanzen ◽

10.1007/s10343-021-00567-1 ◽

2021 ◽

Author(s):

Kamal Lamnai ◽

Fatima Anaya ◽

Rachid Fghire ◽

Hamza Zine ◽

Said Wahbi ◽

...

Keyword(s):

Enzyme Activity ◽

Salicylic Acid ◽

Salt Stress ◽

Antioxidant Enzyme ◽

Water Status ◽

Antioxidant Enzyme Activity ◽

Stress Conditions ◽

Fragaria Vesca ◽

Exogenous Application

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Effect of co-inoculation with arbuscular mycorrhizal fungi and phosphate solubilizing fungi on nutrient uptake and photosynthesis of beach palm under salt stress environment

Scientific Reports ◽

10.1038/s41598-021-84284-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Xue-Ming Zai ◽

Jun-Jun Fan ◽

Zhen-Ping Hao ◽

Xing-Man Liu ◽

Wang-Xiang Zhang

Keyword(s):

Growth Rate ◽

Salt Stress ◽

Nutrient Uptake ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Maximum Efficiency ◽

Poor Growth ◽

Stress Environment ◽

Beach Plum ◽

Phosphate Solubilizing

AbstractBeach plum (Prunus maritima) is an ornamental plant, famous for its strong salt and drought stress tolerance. However, the poor growth rate of transplanted seedlings has seriously restricted its application in salinized soil. This study investigated the effects of inoculation with arbuscular mycorrhizal fungus (AMF), Funneliformis mosseae, and phosphate-solubilizing fungus (PSF), Apophysomyces spartima, on the growth, nutrient (N, P, and K) uptake, and photosynthesis of beach plum under saline (170 mM NaCl) and non-saline (0 mM NaCl) conditions. We aimed to find measures to increase the growth rate of beach plum in saline-alkali land and to understand the reasons for this increase. The results showed that salinization adversely affected colonization by AMF but positively increased PSF populations (increased by 33.9–93.3% over non-NaCl treatment). The dual application of AMF and PSF mitigated the effects of salt stress on all growth parameters and nutrient uptake, significantly for roots (dry weight and P and N contents increased by 91.0%, 68.9%, and 40%, respectively, over non-NaCl treatment). Salinization caused significant reductions in net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and intercellular CO2 concentration (Ci) value, while inoculation with AMF and PSF inoculations significantly abated such reductions. The maximum efficiency of photosystem II (PSII) (Fv/Fm), the photochemical quenching coefficient (qP), and the nonphotochemical quenching (NPQ) values were affected little by inoculation with AMF, PSF, or both under non-NaCl treatments. However, plants inoculated with AMF and/or PSF had higher Fv/Fm, qP, and ФPSII values (increased by 72.5–188.1%) than the control under NaCl treatment, but not a higher NPQ value. We concluded that inoculation with AMF or PSF increased nutrient uptake and improved the gas-exchange and Chl fluorescence parameters of beach plum under salt stress environment. These effects could be strengthened by the combination of AMF and PSF, especially for nutrient uptake, root growth, and Pn, thereby alleviating the deleterious effects of NaCl stress on beach plum growth.

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