scholarly journals In vivo anti-diabetic activity test of ethanol extract of the leaves of Cassia Siamea Lamk

2018 ◽  
Vol 135 ◽  
pp. 632-642
Author(s):  
Heruna Tanty ◽  
Syarifah Diana Permai ◽  
Herena Pudjihastuti
Keyword(s):  
Ethanol Extract ◽  
Cassia Siamea ◽  
Activity Test

scholarly journals Phytochemical Screening and Anti-Hyperuricemia Activity Test In Vivo of Ethanolic Extract of Shallot (Allium cepa L.) Skin

2020 ◽  
Vol 3 (3) ◽  
pp. 146-151
Author(s):  
Syahrina Syahrina ◽  
Vivi Asfianti ◽  
Kasta Gurning ◽  
Iksen Iksen
Keyword(s):  
Uric Acid ◽  
Allium Cepa ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Purine Metabolism ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Allium Cepa L ◽  
Potassium Oxonate

Uric acid is the final product of purine metabolism that will be excreted through urine, feces, and sweat. Excessive production of uric acid can cause hyperuricemia, known as gout. The skin of shallots (Allium cepa L.) is one of the household wastes that are very rarely used by the community. Ethanol extract of shallot skin (EESS) was tested for phytochemical screening and anti-hyperuricemia activity using potassium oxonate. Mice were divided into five groups (Allopurinol, Na-CMC, EESS 200 mg/kg BW, 300 mg/kg BW, and 400 mg/kg BW) and uric acid levels were observed at 2-hour intervals for six hours. Phytochemical screening shows that EESS has potential compounds in the treatment of gout. Tests to reduce uric acid levels showed that EESS has better potential than allopurinol at concentrations of 300 mg/kg BW and 400 mg/kg BW after six hours of induction in reducing uric acid levels.

scholarly journals Efektivitas Kombinasi Ekstrak Etanol Daun Bunga Matahari dan Tanaman Anting-Anting sebagai Antimalaria Secara In Vivo

2019 ◽  
Vol 5 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Khoirul Ngibad
Keyword(s):  
Medicinal Plants ◽  
Human Body ◽  
Antimalarial Activity ◽  
Combination Index ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Activity Test ◽  
Acalypha Indica

Sunflower (Helianthus annuus) and anting-anting (Acalypha indica Linn), can be studied as efficacious malaria medicinal plants for preventing the development of malaria plasmodium resistance. This study aims to determine the effectiveness of the combination of 80% ethanol extract of sunflower leaves and anting-anting as antimalarials. This study included extraction of sunflower leaves and anting-anting plant separately using the maceration method for 24 hours with 80% ethanol. Stirring is aided by a 3 hour shaker and then the obtained extracts were mixed and tested in vivo antimalarial activity against mice test animals. Data of the parasitemia degree was analyzed for the determination of Effective Dose (ED50) using probit analysis then determination of Combination Index (CI) value. The results of the antimalarial activity test on tested mice were ED50 of 1.23 mg/kg human body with a Combination Index (CI) of 0.46 (CI <1) which means synergistic potency.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

Ethanol Extract Of Centella Asiatica (L.) Urban Leaves Effectively Inhibit Streptococcus pyogenes and Pseudomonas aeruginosa by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 69-76
Author(s):  
Dini Aulia Azmi ◽  
Nurlailah Nurlailah ◽  
Ratih Dewi Dwiyanti
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Bacterial Infections ◽  
Herbal Medicines ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Initial Stage ◽  
Independent Variable

Streptococcus pyogenes and Pseudomonas aeruginosa are some of the causes of infectious diseases. Centella asiatica (L.) Urban has many benefits for humans, including overcoming fever, anti-bacterial, and anti-inflammatory. This study aims to determine the inhibition of Centella asiatica (L.) Urban leaves ethanol extract on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. This research is the initial stage of the development of herbal medicines to treat Streptococcus pyogenes and Pseudomonas aeruginosa infections. The independent variable was the concentration of ethanol extract of Centella asiatica (L.) Urban leaves and the dependent variable was the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. The anti-bacterial activity test was carried out by the liquid dilution method. The concentrations used are 20%, 40%, 60%, 80%. 100% The results showed that the minimum inhibitory concentration (MIC) against Streptococcus pyogenes: 40% and Pseudomonas aeruginosa: 40%. Minimum bactericidal concentration (MBC) results for Streptococcus pyogenes: 60% and Pseudomonas aeruginosa: 60%. So it can be concluded that there is inhibition of the ethanol extract of Centella asiatica (L.) Urban leaves on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. Centella Asiatica (L.) Urban extract has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production

2020 ◽  
Vol 21 ◽  
Author(s):  
Hana M. Hammad ◽  
Amer Imraish ◽  
Maysa Al-Hussaini ◽  
Malek Zihlif ◽  
Amani A. Harb ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rural Communities ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Aortic Ring ◽  
Treated Group ◽  
Control Group ◽  
Dependent Manner ◽  
Achillea Fragrantissima

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

scholarly journals Dipterocarpus tuberculatus Roxb. Ethanol Extract Has Anti-Inflammatory and Hepatoprotective Effects In Vitro and In Vivo by Targeting the IRAK1/AP-1 Pathway

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2529
Author(s):  
Haeyeop Kim ◽  
Woo Seok Yang ◽  
Khin Myo Htwe ◽  
Mi-Nam Lee ◽  
Young-Dong Kim ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Related Proteins ◽  
Pro Inflammatory Cytokines ◽  
Inflammatory Effect

Dipterocarpus tuberculatus Roxb. has been used traditionally as a remedy for many diseases, especially inflammation. Therefore, we analyzed and explored the mechanism of the anti-inflammatory effect of a Dipterocarpus tuberculatus Roxb. ethanol extract (Dt-EE). Dt-EE clearly and dose-dependently inhibited the expression of pro-inflammatory cytokines such as IL-6, TNF-α, and IL-1β in lipopolysaccharide (LPS)-treated RAW264.7 cells. Also, Dt-EE suppressed the activation of the MyD88/TRIF-mediated AP-1 pathway and the AP-1 pathway related proteins JNK2, MKK4/7, and TAK1, which occurred as a result of inhibiting the kinase activity of IRAK1 and IRAK4, the most upstream factors of the AP-1 pathway. Finally, Dt-EE displayed hepatoprotective activity in a mouse model of hepatitis induced with LPS/D-galactosamine (D-GalN) through decreasing the serum levels of alanine aminotransferase and suppressing the activation of JNK and IRAK1. Therefore, our results strongly suggest that Dt-EE could be a candidate anti-inflammatory herbal medicine with IRAK1/AP-1 inhibitory and hepatoprotective properties.

scholarly journals High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3977
Author(s):  
Shaoyun Wang ◽  
Xiaozhu Sun ◽  
Shuo An ◽  
Fang Sang ◽  
Yunli Zhao ◽  
...  
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Water Extract ◽  
Ethanol Extract ◽  
In Vivo Studies ◽  
Polygonum Multiflorum ◽  
Q Exactive ◽  
Orbitrap Ms

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.

Identification of Secondary Metabolite Compounds and Cytotoxic Activity Test of Papuan Grape (Sararanga Sinuosa Hemsley) Extract using Brine Shrimp Lethality Test (BSLT) Method

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Elsye Gunawan ◽  
Enrick Kharo Etmond ◽  
Linus Yhani Chrystomo
Keyword(s):  
Cytotoxic Activity ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Organ System ◽  
Activity Test ◽  
Anti Cancer ◽  
Extraction Stage ◽  
Cancer Agent ◽  
Concentration Series

Papua has a diversity of flora species, one of which is the Papuan Grape (Sararanga sinuosa Hemsley). It is commonly used by the Depapre community, Jayapura, as a stamina booster. This research aims to identify the secondary metabolite compounds, to test the cytotoxic activity of Papuan Grape (Sararanga sinuosa Hemsley) extract, and to determine the best concentration that inhabits the growth of Artemia salina larvae using the BSLT method. This study was conducted with the extraction stage using the maceration method by making use of 96% ethanol solvent. Subsequently, the concentration series 0, 50, 100, 150, 200, 250, 300 ppm of Papuan Grape (Sararanga sinuosa Hemsley) extract were made to test the cytotoxic activity on the mortality of Artemia salina shrimp larvae. The results showed that Alkaloids, Flavonoids, Saponins, and Tannins were compounded as secondary metabolite. An antioxidant research that had been carried out previously had LC50 of green-white fruit (12,49 ± 0,35 mg/ml), orange-red fruit (17,62 ± 3,49 mg/ml) and red fruit (12,23 ± 0,46 mg/ml). The community process one stalk of it into juice and used or consumed it two times a day. An inappropriate dose of traditional medicine usage can affect the organ system and had adverse effects in the future The result of cytotoxic research obtained the value of LC50 in ethanol extract of Papuan Grape was 140,863 ppm, and concentration of 250 ppm was the best concentration to inhibit the growth of shrimp larvae (Artemia salina L). The conclusion of this study was the ethanol extract of Papuan Grape (Sararanga sinuosa Hemsley) showed the highest cytotoxic activity and potentially become an anti-cancer agent.

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