Studies on the nature and properties of the perienteric fluid of Ascaris lumbricoides

Parasitology ◽  
1945 ◽  
Vol 36 (3-4) ◽  
pp. 211-218 ◽  
Author(s):  
W. P. Rogers
Keyword(s):  
Ascorbic Acid ◽  
Fatty Acid ◽  
Ascaris Lumbricoides ◽  
Body Fluid ◽  
Uronic Acid ◽  
Amino Sugar ◽  
The Body ◽  
Chief Factors ◽  
Tissue Fluids

1. The composition of the perienteric fluid of Ascaris lumbricoides of the pig, immediately after removal from the host and after varying periods of in vitro starvation, is recorded.2. Apart from the more frequently observed constituents of invertebrate tissue fluids, the body fluid of Ascaris was found to contain ascorbic acid, amino sugar and small amounts of uronic acid.3. Large amounts of anion other than chloride, probably fatty acid, must have been present in the body fluid, though chloride was probably the predominant anion in fluids of parasites which had passed several days of in vitro life.4. The chief factors (starvation, osmotic pressure and the nature of the medium, etc.) affecting the composition of the body fluid are briefly discussed.

Studies on the Physiology of Ascaris Lumbricoides

1952 ◽  
Vol 29 (1) ◽  
pp. 22-29
Author(s):  
A. D. HOBSON ◽  
W. STEPHENSON ◽  
A. EDEN
Keyword(s):  
Ascaris Lumbricoides ◽  
Body Fluid ◽  
External Medium ◽  
Chloride Concentration ◽  
The Body ◽  
Considerable Proportion ◽  
Limiting Factor ◽  
Inorganic Cations ◽  
Sodium And Potassium ◽  
Total Concentrations

The results obtained in this investigation are admittedly not as extensive as is desirable but they allow certain conclusions to be drawn. 1. The sodium and potassium contents of the body fluid of Ascaris lumbricoides are somewhat variable, but these variations do not seem to be dependent upon those of the external medium. 2. The calcium and magnesium contents of the body fluid are relatively constant and are not affected by those of the external medium. 3. The chloride concentration of the body fluid is closely related to and always remains lower than that of the external medium. 4. As shown in Table 2, there is a large gap between the total concentrations of inorganic cations and anions in the intestinal fluid of the pig. Presumably a considerable proportion of the inorganic cations are combined with organic anions, at present undetermined. Exposing the worms to saline media composed of chloride caused a large rise in the internal chloride concentration. This may well be a limiting factor in the life of the animals in such media, and the next step forward would seem to be the fuller analysis of the environment to which they are normally exposed.

Effect of Acid Stress on the Behavior of Soil Nematodes

2014 ◽  
Vol 522-524 ◽  
pp. 341-345
Author(s):  
Dan Dan Liu ◽  
Yan Ling Cui ◽  
Chang Feng Liu ◽  
Xin Lin Gao ◽  
Yin Yao Xia
Keyword(s):  
Body Fluid ◽  
Acid Stress ◽  
Optical Microscope ◽  
The Body ◽  
Individual Development ◽  
Movement Behavior ◽  
Soil Nematode ◽  
In Vitro Test ◽  
Stylet Length

In vitro test was used to determine the effect of acid stress on soil nematode (Meloidogyne incognita) J2 survival, behavior, individual development and fluid extravasations. The effects of acid stress on J2 survival is C2H2O4>C6H8O7>C4H6O5. Inhibition on J2 movement behavior increased with time prolonged. Effect on nematode body length, stylet length, tail transparent area length, body fluid extravasations: C2H2O4>C6H8O7>C4H6O5. At high magnification optical microscope can be clearly observed symptoms of poisoning J2. Compared with the control, acid inhibited the nematode survival, movement and individual development, promote the body fluid extravasations, destroyed the normal physiological metabolism of nematodes, even lead to death.

Accuracy and Precision Study: Body Fluid White Blood Cell (WBC) Analysis (Peritoneal, Pleural and Peritoneal Dialysate) Using a Light Scatter Technology (ADVIA®2120/2120i) Versus Hemocytometer Manual Counts

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4730-4730 ◽  
Author(s):  
Donna D Castellone ◽  
Ellinor I.B. Peerschke ◽  
Nenita Francisco ◽  
William Canfield ◽  
Gail Kling
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Body Fluid ◽  
The Body ◽  
Light Scatter ◽  
Laboratory Assessment ◽  
Peritoneal Dialysate ◽  
Manual Method ◽  
Reproducibility Study

Abstract Abstract 4730 Background: The ADVIA® 2120/2120i body fluid application is an in vitro diagnostic test for the enumeration of the total nucleated cell (TNC) or white blood cell (WBC) count and RBC (red blood cell) count for pleural, peritoneal and peritoneal dialysis (PD) specimens collected in K2 EDTA. Total nucleated cell (TNC) and RBC counts are used in the laboratory assessment of these fluids. The TNC count is an absolute count of the nucleated blood cells, mesothelial cells, and other non-hematopoietic cells in these fluids. Objective: The objective is to demonstrate concordance between the manual (hemocytometer) method versus the light scatter principle used on the ADIVA® 2120i analyzer. Methods: The ADVIA® 2120/2120i Body Fluid Application uses the Basophil/Lobularity and RBC/PLT channels to enumerate the WBC and RBC counts. WBC counts are derived from the Basophil/Lobularity channel by a process in which the cytoplasmic membrane is stripped and the suspension of cells is intercepted by light from the laser diode where the low-angle light scatter (2° to 3°) and high-angle light scatter (5° to 15°) signals of each cell are counted. The manual method uses a hemocytometer in which a chamber is charged with fluid and both areas of the chamber are counted and the result averaged. De-identified remnant peritoneal, pleural and peritoneal dialysate samples were processed within 2 hours in the Hematology Laboratory according to their standard operating procedure. Samples with TNC counts that fell in the range of 0.030 to 400 × 103 cells/μ L (30 to 400,000) for each type of fluid were included. Ten consecutive runs were performed for each type of fluid to determine precision. Results: The WBC reproducibility study (n=10) demonstrates that within the run, precision of the body fluid counts demonstrates a coefficient of variation (CV) ≤ 10 CV. Regression analysis (n=34) demonstrates an r=.93 using samples across the reportable range. Conclusion: These results suggest that the light scatter technology used on the ADVIA® 2120/2120i gives both accurate and precise WBC determinations and compares very well to the manual method for determination of WBC analysis in peritoneal, peritoneal, dialysate, and pleural fluids. Utilizing this method will decrease turn-around time for this process, resulting in a faster time to result and ultimately better patient outcomes. Disclosures: Castellone: Siemens Healthcare: Employment. Canfield:Siemens: Employment. Kling:Siemens: Employment.

scholarly journals Potential Antioxidant Activity of New Tetracyclic and Pentacyclic Nonlinear Phenothiazine Derivatives

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Godwill Azeh Engwa ◽  
Eugene Lekem Ayuk ◽  
Benardeth Ujunwa Igbojekwe ◽  
Marcellus Unaegbu
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Membrane Damage ◽  
The Body ◽  
Phenothiazine Derivatives ◽  
Antioxidant Agents ◽  
Group 3

The global increase in oxidative stress related diseases such as cancer, cardiovascular, and inflammatory diseases caused by overwhelming level of free radicals in the body has encouraged the search for new antioxidant agents. Based on the ability of newly synthesized phenothiazine derivatives (6-chloro-11-azabenzo[a]phenothiazine-5-one and 6-[4-bromophenyl]-10-methyl-11-azabenzo[a]phenothiazine-5-one) to oxidize H2O2, a known free radical to sulfoxide, this study assessed the in vitro and in vivo antioxidant activity. The synthesized phenothiazine derivatives exhibited reducing power potential to convert Fe3+to Fe2+and high ability to scavenge H2O2free radical in vitro. These activities were comparable to ascorbic acid, a standard antioxidant. The catalase activity significantly increased (p<0.05) in groups 1 and 2 animals that received the phenothiazine derivatives compared to the controls (groups 3 and 4) suggesting the ability of the phenothiazine derivatives to scavenge H2O2in vivo. The malondialdehyde level in groups 1 and 2 animals was lower than that in group 3 that received the reference compound (ascorbic acid) and group 4 that received the solvent suggesting the ability of the phenothiazine derivatives to prevent lipid membrane damage. AST and bilirubin levels were higher in group 2 animals which received 6-[4-bromophenyl]-10-methyl-11-azabenzo[a]phenothiazine-5-one compared to group 3, the positive control. The results suggest that phenothiazine derivatives, especially 6-chloro-11-azabenzo[a]phenothiazine-5-one, possess antioxidant activity though 6-[4-bromophenyl]-10-methyl-11-azabenzo[a]phenothiazine-5-one was slightly toxic. This activity may be due to the presence of electron donors such as sulfur as well as the richness of hydrogen in the additional benzene rings for substitution. Further study is needed to identify tolerable doses for possible therapeutic purposes.

scholarly journals Vitamin E and stress

1967 ◽  
Vol 21 (1) ◽  
pp. 69-101 ◽  
Author(s):  
J. Green ◽  
A. T. Diplock ◽  
J. Bunyan ◽  
D. Mchale ◽  
I. R. Muthy
Keyword(s):  
Fatty Acids ◽  
Ascorbic Acid ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Vitamin E ◽  
Dietary Fat ◽  
Methyl Esters ◽  
Cod Liver Oil ◽  
Deficient Diet

1. A critical analysis of the biological antioxidant theory of vitamin E function has been made and the implications of the theory have been tested.2. When small amounts of [5-Me-14C]α-tocopherol were present in lipid systems subject to autoxidation in vitro, it was found that, whether the tocopherol was the sole antioxidant or was in synergistic combination with a secondary antioxidant (ascorbic acid), peroxidation did not occur without concomitant destruction of the tocopherol. This was so, whether a simple fat substrate or a liver homogenate (subject to catalysis) was used. The decomposition of tocopherol took place even when the secondary antioxidant was in large excess, as would occur under physiological conditions in the vitamin E-deficient animal, and accelerated as the induction period neared its end.3. When [5-Me-14C,3H]α-tocopherol and ascorbic acid were used as a synergistic antioxidant couple in vitro, tocopherol recovered from the peroxidizing system always had the same isotopic ratio as the starting material. This means that regeneration of tocopherol by the secondary antioxidant cannot involve, as an intermediate, a tocopherol carbon radical formed by loss of hydrogen from the 5-methyl group. Such radicals probably dimerize before they can be regenerated. The same result was found when doubly labelled α-tocopherol was given to the rat and recovered later from its tissues.4. In a series of experiments, rats were rigorously depleted of vitamin E for periods up to 7 months and then given as little as 50 μg [14C]D-α-tocopherol. They were then given, either by stomach tube daily or by dietary addition, large amounts of methyl linoleate or vitamin E-free polyunsaturated fatty acid methyl esters prepared from cod-liver oil and compared with controls given methyl oleate for up to 31 days. When the possibility of interaction between the lipid and tocopherol in the gut was eliminated, analyses of liver, kidney, testis, adrenal, adipose tissue, whole carcass and faeces showed that there was no effect of the polyunsaturated fatty acids on either the metabolism or recovery of [14C]α-tocopherol in any of the animals.5. When interaction between the administered fatty acid esters and tocopherol in the gut was allowed to take place, a marked destruction of [14C]α-tocopherol in the tissues was observed in animals given the polyunsaturated esters. The importance of oxidative destruction of tocopherol in the gut before absorption was demonstrated in a nutritional trial, in which cod-liver oil and lard were compared and the degrees of resistance of rats' erythrocytes to dialuric acid-induced haemolysis was used as an index of vitamin E depletion.6. Similar experiments with [14Cα-tocopherol in weanling rats given large amounts of cod-liver oil methyl esters also showed little effect. Although there was a suggestion that prolonged feeding of partly peroxidized polyunsaturated esters could lead to a slight depression of tissue tocopherol concentrations, no significant differences were usually obtained.7. Fourteen-day-old rats were given a vitamin E-deficient diet and received three weekly doses of 0.5 mg α-tocophcryl acetate. The dosage was stopped, the rats were then given a deficient diet containing 4% of either vitamin E-free linseed oil fatty acids or oleic acid, and the rate of their tocopherol depletion was measured by the erythrocyte haemolysis test. No effect of the polyunsaturated fatty acids was found. Nor was there any effect on the concentrations of ‘secondary antioxidants’ (glutathione and ascorbic acid) in liver, kidney, testis, muscle or adipose tissue.8. The results of the experiments in vivo contrast strongly with those in vitro. They lead to the conclusion that lipid peroxidation, if it occurs in the living animal, is irrelevant to the problem of vitamin E function. This conclusion has been substantiated by a critical review of the literature on the quantitative aspects of the vitamin E-dietary fat relationship.9. The effects of dietary fat stress in vitamin E-deficient animals are, we believe, due to two causes: (1) destruction of tocopherol in the diet or in the gastro-intestinal tract of the animal, and (2) the existence of an increased requirement for vitamin E for the metabolism of certain long-chain fatty acids. The specific effects of certain of these substances in producing or accelerating some vitamin E deficiency diseases may be related to the toxic states known to be induced in vitamin E-deficient animals by other stress factors.

scholarly journals Metabolism and pharmacokinetics of a novel polyphenol fatty acid ester phloridzin docosahexaenoate in Balb/c female mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wasundara Fernando ◽  
Kerry B. Goralski ◽  
David W. Hoskin ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Fatty Acid ◽  
Cellular Uptake ◽  
Fatty Acid Ester ◽  
Acid Ester ◽  
Biological Effects ◽  
The Body ◽  
Pharmacokinetic Parameters ◽  
Female Mice ◽  
Organ Systems

AbstractFlavonoids are known to undergo phase II metabolism and produce metabolites with similar or stronger biological effects compared to the parent flavonoids. However, the limited cellular uptake and bioavailability restrict their clinical use. We synthesized phloridzin docosahexaenoate (PZ-DHA), a novel fatty acid ester of polyphenol, through an acylation reaction with the aim of increasing the cellular availability and stability of the parent biomolecules, phloridzin (PZ) and docosahexaenoic acid (DHA). Here, we report metabolites and pharmacokinetic parameters of PZ-DHA, determined using ultra-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. PZ-DHA was taken-up by human (MDA-MB-231, MDA-MB-468, and MCF-7) and mouse (4T1) mammary carcinoma and human non-malignant mammary epithelial cells (MCF-10A) in cellular uptake assays. Our results suggested that the acylation improves the cellular uptake of PZ and stability of DHA within cells. In mouse hepatic microsomal assays, two major glucuronides of PZ-DHA, PZ-DHA-4-O-glucuronide and PZ-DHA-4′-O-glucuronide (MW = 923.02 g/mol), were detected. One tri-methylated- (4,4′,6′-O-trimethyl-PZ-DHA) (MW = 788.88 g/mol) and one di-sulphated- (PZ-DHA-4,4′-O-disulphide) PZ-DHA metabolite (MW = 906.20 g/mol) were also identified. Intraperitoneal injections of PZ-DHA (100 mg/kg) into Balb/c female mice was rapidly absorbed with a serum Cmax and Tmax of 23.7 µM and 60 min, respectively, and rapidly eliminated (t1/2 = 28.7 min). PZ-DHA and its metabolites are readily distributed throughout the body (Vd = 57 mL) into many organs. We identified in vitro and in vivo metabolites of PZ-DHA, which could be tested for potential use to treat diseases such as cancer in multiple organ systems.

scholarly journals Anti-Obesity Effects of Macroalgae

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2378 ◽  
Author(s):  
Saioa Gómez-Zorita ◽  
Maitane González-Arceo ◽  
Jenifer Trepiana ◽  
Itziar Eseberri ◽  
Alfredo Fernández-Quintela ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Body Fat ◽  
Overweight And Obesity ◽  
The Body ◽  
De Novo Lipogenesis ◽  
Acid Oxidation ◽  
Food Ingredients ◽  
Pharmaceutical Industries

Macroalgae have attracted great interest for their potential applications in nutraceutical and pharmaceutical industries as source of bioactive medicinal products and food ingredients. This review gathers data from in vitro and in vivo studies addressing the anti-obesity effects of macroalgae. Great consensus exists in all reported in vitro studies concerning the reduction induced by seaweed extracts in the expression of transcriptional factors controlling adipogenesis. In animals, macroalgae reduced body fat accumulation and prevented other obesity features, such as dyslipidemia, insulin resistance and fatty liver. These effects are not due to food intake reduction, since few studies have reported such event. Indeed, the effects on metabolic pathways in target tissues/organs seem to play a more relevant role. Macroalgae can reduce de novo lipogenesis, limiting fatty acid availability for triglyceride synthesis in white adipose tissue. This effect has been observed in both cell cultures and adipose tissue from animals treated with macroalgae extracts. In addition, increased fatty acid oxidation and thermogenic capacity, as well as a shift towards healthier gut microbiota composition may contribute to the body fat-lowering effect of macroalgae. Studies in humans are needed to determine whether macroalgae can represent a feasible tool to prevent and/or manage overweight and obesity.

Ketone Body Metabolism in Ascorbic Acid Deficiency

1956 ◽  
Vol 185 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Albert F. Debons ◽  
John W. Wallace ◽  
Habeeb Bacchus
Keyword(s):  
Ascorbic Acid ◽  
Fatty Acid ◽  
Guinea Pig ◽  
Ketone Bodies ◽  
Control Pair ◽  
Fatty Acid Substrate ◽  
Ascorbic Acid Deficiency ◽  
Spontaneous Production ◽  
Acid Deficiency

The in vitro formation of ketone bodies by liver tissues of ascorbic acid-deficient, and control (pair-fed) guinea pigs was studied. The data revealed that the spontaneous production of ketone bodies by the liver of the ascorbic acid-deficient guinea pig is less than that of the control animal. The capacity of the liver of the deficient guinea pig to form ketone bodies from octanoate exceeds that of control animals. The total fat content and the liver weight are not significantly altered in ascorbic acid-deficiency. The data suggest that the diminished spontaneous production of ketone bodies by the ascorbic acid-deficient liver, in spite of its normal content of fat, might be secondary to a diminished fatty acid formation from endogenous fat. In the presence of fatty acid substrate the formation of ketone bodies by the ascorbic acid-deficient liver is not appreciably disturbed.

An Isolated Nerve-Muscle Preparation from Ascaris Lumbricoides

1947 ◽  
Vol 23 (3-4) ◽  
pp. 277-291
Author(s):  
ERNEST BALDWIN ◽  
VIVIEN MOYLE
Keyword(s):  
Ascaris Lumbricoides ◽  
Body Fluid ◽  
Body Wall ◽  
Physiological Activity ◽  
Surrounding Medium ◽  
The Body ◽  
Muscle Preparation ◽  
Nerve Muscle

1. A technique is described for the preparation from the body wall of Ascaris of semi-isolated strips of muscle. These strips are exposed on one side to the surrounding medium and are suitable for studies of the action of anthelminthic and other drugs upon the exposed musculature. 2. A medium suitable for use in such experiments has been devised and its preparation is described. 3. Media made up to represent the body fluid of Ascaris fail to support physiological activity in the exposed muscle strips, and it seems that this perienteric fluid does not correspond to the true milieu intérieur of this nematode. 4. Some new observations on the nature and composition of the perienteric fluid are presented incidentally in the text.

scholarly journals Identification of Differentially Expressed Genes for Lipid Metabolism in Dedifferentiated Preadipocytes from Different Tissues of Chicken

2020 ◽  
Author(s):  
zheng ma ◽  
Na Luo ◽  
Lu Liu ◽  
Huanxian Cui ◽  
Jing Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
The Body ◽  
Fatty Acid Transport ◽  
Lipid Deposition ◽  
Tgf Beta ◽  
Body Distribution ◽  
Ppar Signaling

Abstract Background: The body distribution with high intramuscular fat and low abdominal fat is ideal goal for broiler breeding. Preadipocytes with different origins have differences in metabolism and gene expression. This transcriptome analysis of intramuscular preadipocytes (DIMPs) and adipose tissue-derived preadipocytes (DAFPs) is aim to explore the characteristics in lipid deposition of different chicken preadipocytes by dedifferentiation in vitro. Results: Compared to DIMFPs, the lipid content was increased (P <0.05) in DAFPs after two days with 100% confluence. Moreover, 66 DEGs of lipid metabolism were screened, which are involved in the adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among them, the representative CEBPA, DGKH, DGKQ, DGKD, FADS1L1, SCD, SCD5, and PPARG were down-regulated, but CIDEC, ELOVL1, ELOVL6, FABP3, FABP4, FADS6, LPL, MOGAT1, PLIN3, PLIN4, RBP7, and RXRG genes were up-regulated (P < 0.05 or P < 0.01) in the DAFPs, showing the same pattern with the lipid content. Based on the known DEGs, the well-known pathways affecting lipid metabolism (MAPK-, TGF beta-, Calcium-, PPAR signaling pathway) were enriched, which may also contribute to the regulation of lipid deposition. Conclusions: Our data suggest that the difference of lipid deposition between DIMPs and DAFPs of chicken in vitro. The lipid content was significantly increased in DAFPs by the up-regulation of genes on cellular uptake of fatty acids through medication of MAPK-, TGF beta-, Calcium-, and PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and optimizing body fat distribution in broilers.

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