scholarly journals The effectiveness of Rutin for prevention of surgical induced endometriosis development in a rat model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hatef Talebi ◽  
Mohammad Reza Farahpour ◽  
Hamed Hamishehkar
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Rat Model ◽  
Tunel Staining ◽  
Caspase 9 ◽  
Super Oxide Dismutase ◽  
Total Antioxidant ◽  
Blotting Technique ◽  
First Time ◽  
Antioxidant Mechanisms

AbstractApoptosis and antioxidant mechanisms are pathways for the treatment of endometriosis (Endo). Rutin (Rtn) is an antioxidant flavonol that induces apoptosis. This study, for first time, was conducted to evaluate the effects of rutin on Endo through apoptosis and antioxidant mechanisms. The experimental Endo was induced in 24 rats and then the animals were subdivided into Endo-sole, 3000 and 6000 µg/kg rutin (Rtn-3000 and Rtn-6000) and vitamin C groups. After 4 weeks, the expression of Bcl2, Bax, anti Pro Caspase-9, cleaved Caspase-9, pro PARP, pro Cleaved PARP, Pro PARP, pro mTOR and mTOR were assessed by western blotting technique. The protein concentrations of malondialdehyde (MDA), total antioxidant capacity, and super oxide dismutase and gutathione peroxidase were also evaluated. TUNEL staining was also used for the detection of apoptosis. Caspase-9 and concentration of antioxidants were higher in the treated groups compared to Endo-sole group (P < 0.05). The results also showed that rutin decreased the expression of Bcl2 and MDA concentration (P < 0.05). The results for TUNEL staining showed that the animals treated with Rtn-6000 and vitamin C showed higher apoptosis. Rutin induces apoptosis by the expression of Bcl-2, Bax and caspase and also antioxidant activity by increasing antioxidants concentrations.

Changes in the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of their fattening

2020 ◽  
Vol 1 (12) ◽  
pp. 112-118
Author(s):  
O. A. Voronina ◽  
◽  
A. A. Savina ◽  
N. S. Kolesnik ◽  
N. V. Bogolyubova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Water Soluble ◽  
Laboratory Studies ◽  
Blood Samples ◽  
Amperometric Method ◽  
Significant Difference ◽  
Total Antioxidant ◽  
First Time

The influence of fattening conditions on the digestive and metabolic processes in animals is actively studied to increase the production of animal food. The aim of the study was to estimate the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of fattening. Experiments were conducted on 102 animals to confirm the results of laboratory studies, as well as determination of the total antioxidant activity of pig blood serum by amperometric method. For the first time, a significant difference in antioxidant activity was found in blood samples of Duroc boars with the duration of their fattening from 81 to 101 days.

scholarly journals Effect of mineral fertilizers on the variability of phytochemicals in kale

2021 ◽  
Vol 1 (43) ◽  
pp. 8-8
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Antioxidant Activity ◽  
Trace Elements ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Mineral Nutrients ◽  
Mineral Fertilizers ◽  
Maturity Stage ◽  
Biochemical Compounds ◽  
Brassica Vegetables ◽  
Total Antioxidant

The effect of five mineral nutrients on the variability of the content of biochemical compounds, such as flavonoids, phenolic compounds, vitamin C, carotenoids and the antioxidant activity of kale (Brassica Oleracea var. sabellica) collected at the edible maturity stage. Manipulation of the phytochemical composition of the kale cabbage using mineral fertilizers was evident in the results achieved. The various mineral nutrients used had different effects on the content of the studied phytochemicals. The content of flavonoids and carotenoids did not decrease with any of the various mineral nutrients used. Contrary to recent research suggesting that rare earth elements are good stimulators of secondary metabolite accumulation, our results showed a decrease in the content of phenolic compounds in plants fertilized with neodymium. The total antioxidant activity increased under the influence of NPK and humate, which correlated with the highest content of soluble phenolic compounds and flavonoids in kale leaves. Low doses of N have a positive effect on these indicators. Mineral supplements of trace elements also had a stimulating effect on the accumulation of the studied substances. However, in addition to trace elements, a significant role is played by the presence of humic acids (in humate), which together with trace elements have the maximum stimulating effect on the content of the substances under study. Keywords: MINERAL FERTILIZERS, ANTIOXIDANT PHYTOCHEMICALS, PHENOLIC COMPOUNDS, CAROTENOID, ANTIOXIDATIVE ACTIVITY, VITAMIN C, FLAVONOIDS, BRASSICA VEGETABLES

scholarly journals Nutrition and bioactive potential of the noni fruit cultivated from the Mato Grosso State

2021 ◽  
Vol 51 (2) ◽  
Author(s):  
Ilmara Pereira de Vasconcelos ◽  
Rodrigo Esaú Vassoler Silva ◽  
Patrícia Monique Crivelari Costa ◽  
Luiz José Rodrigues
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Protein Content ◽  
Morinda Citrifolia ◽  
Chemical Characteristics ◽  
Total Antioxidant Activity ◽  
Mato Grosso ◽  
Total Antioxidant ◽  
Bioactive Potential ◽  
Physical And Chemical

ABSTRACT: The objective was to evaluate the physical and chemical characteristics of noni (Morinda citrifolia) grown in the Mato Grosso State. Ripe fruits from a field located in Cuiabá-MT, had the peel, pulp and seeds separated and subjected to different evaluations. The fruit parts were characterized physically and chemically. Determinations of vitamin C, carotenoids, phenolics and the total antioxidant activity of the fruit mesocarp (pulp) were made. Noni cultivated in the Mato Grosso State presents high levels of potassium, calcium and sodium, with predominance of potassium. The protein content was higher in the seed, and the ash, in the peel, respectively. Noni pulp showed high levels of vitamin C, carotenoids and phenolics and can be considered a potential antioxidant.

scholarly journals Phytochemical Diversity in Rhizomes of Three Reynoutria Species and their Antioxidant Activity Correlations Elucidated by LC-ESI-MS/MS Analysis

2019 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sylwester Ślusarczyk ◽  
Sebastian Granica ◽  
Jakub Hadzik ◽  
Adam Matkowski
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Therapeutic Effects ◽  
Similar Species ◽  
Reynoutria Japonica ◽  
Esi Ms ◽  
Total Antioxidant ◽  
First Time

One of the richest natural sources of resveratrol - the rhizome of Reynoutria japonica in East Asia is a well-known traditional herb (Hu zhang) used in various inflammatory diseases, infections, skin diseases, scald, hyperlipidemia. Although, it has been recently included in the European Pharmacopoeia, still in Europe is an untapped resource. Some of the therapeutic effects are likely to be influenced by its antioxidant properties and this in turn is frequently associated with a high stilbene content. However, some literatures suggested that other compounds than stilbenes may add to the total antioxidant capacity. Hence, the aim of this research was to examine rhizomes of R. japonica and less studied, morphologically similar species, R. sachalinensis and R. x bohemica for their phytochemical composition and antioxidant activity and to clarify the relationship between the antioxidant activity and compounds by statistical methods. HPLC/UV/ESI-MS studies of three Reynoutria species revealed 171 compounds comprising stilbenes, carbohydrates, procyanidins, flavan-3-ols, anthraquinones, phenylpropanoids, lignin oligomers, hydroxycinnamic acids, naphthalenes and their derivatives. Our studies confirmed the presence of procyanidins with high degree of polymerization, up to decamers in the rhizomes of R. japonica and brings new data on the presence of these compounds in other Reynoutria species. A procyanidin trimer digallate was described for the first time in the studied plants. Moreover, we suggested a presence of new for these species, dianthrone glycosides and previously unrecorded phenylpropanoid disaccharide esters and hydroxycinnamic acid derivatives, mainly in R. sachalinensis. Furthemore, compounds tentatively annotated as lignin oligomers were observed for the first time in studied species. The rhizomes of all Reynoutria species exhibited strong antioxidant activity. Statistical analysis demonstrated that proanthocyanidins should be considered as important contributors to the total antioxidant capacity.

scholarly journals Aktivitas Antioksidan Fraksi Etil Asetat Dari Ekstrak Metanol Sargassum sp.

2019 ◽  
Vol 8 (1) ◽  
pp. 41-46
Author(s):  
Hafida Salma ◽  
Sri Sedjati Sedjati ◽  
Ali Ridlo
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Rotary Evaporator ◽  
Total Antioxidant

Sargassum sp. adalah salah satu jenis rumput laut paling banyak di Indonesia dan memiliki manfaat sebagai antioksidan, obat penyakit jantung, stroke, dll. Penelitian ini bertujuan untuk menentukan kandungan antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. Sampel diambil dari Pantai Sundak, Gunung Kidul, Yogyakarta. Metode yang digunakan adalah eksploratif diskriptif. Sampel dikeringkan dalam suhu ruangan selama 7 hari dan dimaserasi dengan pelarut metanol, lalu dievaporasi dengan rotary evaporator. Ekstrak metanol di fraksinasi menggunakan etil asetat. Pengujian aktivitas antioksidan dilakukan dengan menggunakan 2 metode, yaitu metode penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Vitamin C digunakan digunakan sebagai standar penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Kadar total fenolat diuji menggunakan larutan Folin-Ciocalteu dengan standar asam galat. Hasil penelitian menunjukkan bahwa kadar total fenolat fraksi etil asetat Sargassum sp. sebesar 64,42 mg GAE/g sampel. Aktivitas antioksidan dengan metode penangkapan radikal bebas DPPH memiliki nilai IC50 1.289 ppm, vitamin C memiliki IC50 sebesar 122,71 ppm, sedangkan total kapasitas antioksidan adalah 39,52 mg AAE/g sampel. Kesimpulannya, yaitu kandungan aktivitas antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. dengan metode penangkapan radikal bebas DPPH diduga sangat lemah dan kandungan aktivitas antioksidan dengan menggunakan metode fosfomolibdat diduga tinggi.  Sargassum sp. is one of the many types of seaweed in Indonesia and has benefits as an antioxidant, a drug for heart disease, stroke, etc. This study aims to determine the antioxidant content of ethyl acetate fraction of methanol extract of Sargassum sp. Samples were taken from Sundak, Gunung Kidul, Yogyakarta. The method used is descriptive explorative. Samples were dried at room temperature for 7 days and macerated with methanol, then evaporated at a rotary evaporator. The methanol extract fractionation using ethyl acetate. Testing the antioxidant activity using two methods, the method of catching free radicals DPPH and total antioxidant capacity fosfomolibdat. Vitamin C is used as a standard arrest DPPH free radical and total antioxidant capacity fosfomolibdat. Levels of total phenolics were tested using the Folin-Ciocalteu solution with gallic acid standard. The results showed that levels of total phenolic fraction of ethyl acetate Sargassum sp. amounting to 64,42 mg GAE/g sample. The antioxidant activity with catching free radicals DPPH methods have IC50 values 1.289 ppm, vitamin C has an IC50 of 122,71 ppm, while the total antioxidant capacity was 39,52 mg AAE/g sample. In conclusion, the content of the antioxidant activity of ethyl acetate fraction of methanol extract of Sargassum sp. with catching free radicals DPPH methods allegedly extremely weak and the content of the antioxidant activity using methods fosfomolibdat predictably high.

scholarly journals Evaluation of oxidative stress and antioxidant activity in pre and post hemodialysis in chronic renal failure patients from Western region of Nepal

2013 ◽  
Vol 13 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Takkella Nagamma ◽  
S Ahmed ◽  
Akshatha Pai ◽  
Sheethal Mohan ◽  
Abhishek Chathuvedi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Renal Failure ◽  
Chronic Renal Failure ◽  
Vitamin C ◽  
Oxidative Stress Marker ◽  
Cellulose Membrane ◽  
Total Antioxidant Activity ◽  
Plasma Urea ◽  
Total Antioxidant

Objective: To evaluate the oxidative stress and antioxidant activity in pre and post hemodialysis in chronic renal failure patients using a diacetate cellulose membrane. Background: Chronic Renal Failure is a gradual, progressive and irreversible loss of normal functioning of kidneys. This is associated with increased formation of reactive oxygen species and increased oxidative stress. No studies have been undertaken in this area in Nepal. Method: The study groups were devided into chronic renal failure patients undergoing hemodialysis (n=52) and healthy controls (n=52). Diacetate cellulose was used as dialyzer membrane for chronic renal failure patients. Blood samples were collected in EDTAbottles and plasma was used for the estimation of oxidative stress marker, total antioxidant activity, vitamin C, ?-tocopherol, urea and creatinine. Results: There was significant reduction in the levels of vitamin C,? -tocopherol and total antioxidant activity in post dialysis patients compared to predialysis and control groups (p<0.001). Plasma urea and creatinine levels were high in predialysis patients compared to controls and was significantly reduced after dialysis. Significantly elevated malondialdehyde levels were found in chronic renal failure patients before and after dialysis when compared with controls. Conclusion: The results of our study indicate that there are reduced levels antioxidants in chronic renal patients in pre and post dialysis condition and also more loss of antioxidants after dialysis. All these condition leads to increased oxidative stress in these patients. DOI: http://dx.doi.org/10.3329/bjms.v13i1.14741 Bangladesh Journal of Medical Science Vol. 13 No. 01 January2014: 40-44

scholarly journals Acetylcholinesterase Inhibitory and Antioxidant Activity of the Compounds Isolated from Vanda roxburghii

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Salim Ahammed ◽  
Rejina Afrin ◽  
Nasim Uddin ◽  
Yusuf Al-Amin ◽  
Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nervous System ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Fraction ◽  
Total Antioxidant Activity ◽  
Traditional Use ◽  
Phytochemical Investigation ◽  
Total Antioxidant ◽  
First Time

Vanda roxburghii has been used in traditional medicine to treat nervous system disorders including Alzheimer’s disease (AD). We reported earlier a high acetylcholinesterase inhibitory and antioxidant activity in the chloroform fraction of this plant. Therefore, this study was designed to explore the compounds with acetylcholinesterase inhibitory and antioxidant activities from the chloroform fraction of Vanda roxburghii. Phytochemical investigation led to the isolation for the first time of a fatty acid ester: methyl linoleate (1), and three phenolics: syringaldehyde (2), vanillin (3), and dihydroconiferyl dihydro-p-coumarate (4) along with the previously reported compound gigantol (5). Among the isolates, vanillin (3) and dihydroconiferyl dihydro-p-coumarate (4) were found to significantly inhibit the activity of acetylcholinesterase, scavenge the free radicals, exhibit the reducing power and total antioxidant activity, and effectively reduce the peroxidation of lipid. Gigantol (5) and syringaldehyde (2), despite lacking the activity against acetylcholinesterase, exhibited antioxidant activity. Among the compounds, gigantol (5) appeared to be the most potent antioxidant. These findings revealed that V. roxburghii contained compounds with potential acetylcholinesterase inhibitory and antioxidant activity, which support its traditional use in the treatment of AD.

scholarly journals Physicochemical and Bioactive Characterisation of Edible and Waste Parts of “Piel de Sapo” Melon

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 60 ◽  
Author(s):  
Fátima Alves Miller ◽  
Joana Freitas Fundo ◽  
Ester Garcia ◽  
João Rodrigo Santos ◽  
Cristina Luisa Miranda Silva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Total Phenolics ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Soluble Solids ◽  
Total Carotenoids ◽  
Total Antioxidant ◽  
Solids Content

Several scientific studies point fruits as rich sources of antioxidants but mainly focus on their edible part. However, fruits wastes are abundant sources of bioactive compounds and nutrients, which are considered to be health beneficial. The main purpose was to characterise juice, pulp, peel and seeds of Piel de Sapo melon, in terms of several physicochemical characteristics (soluble solids content, titratable acidity, pH, potassium, colour and water activity), some bioactive compounds (total phenolics, vitamin C, chlorophylls and total carotenoids) and total antioxidant activity. Juice, pulp, peel and seeds represent 47, 19, 27 and 5% of melon total weight, respectively. Peel and seeds stood out by their higher concentration of total phenolics compounds and antioxidant activity when compared to edible parts. The highest potassium concentration was found in seeds. Chlorophylls were only detected in peel, while carotenoids were not detected in any part of the melon analysed. Juice and pulp contributed to 69% of vitamin C amount of the whole fruit. However, its concentration in peel was equivalent to the ones observed in juice and pulp. These results pointed out the importance of fruit wastes valorisation and the development of strategies for their re-utilisation.

scholarly journals Fruit of raspberry Rubus Idaeus L. as a source of functional ingredients (review)

2019 ◽  
Vol 48 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Екатерина Жбанова ◽  
Ekaterina Zhbanova
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Properties ◽  
Rubus Idaeus ◽  
Anthocyanin Content ◽  
Total Antioxidant Activity ◽  
Red Raspberry ◽  
Total Anthocyanin ◽  
Wide Range ◽  
Total Antioxidant

The article gives a comparative analysis of the data obtained by Russian and foreign scientists considering the content of vitamins and other valuable substances in raspberry fruit. That determines high antioxidant properties of raspberry. The goal of the study was to generalize information which shows specific nature of that plant in relation to the complex of vitamins and polyphenol compounds which it contains. The author shows that fruit of raspberry R. idaeus L. consists of 5–40 mg/100 g of vitamin C, folic acid - 26-44 mg/100 g. Consumption of only 100 g of raspberries satisfies daily requirement of vitamin C (60 mg) by 8.3 - 66.7%, in folates (200 mg) by 13–22%. Total anthocyanin content in red raspberry varies within wide range (20–100 mg/100 g), in black raspberry – form 200 to 600 mg/100 g. Raspberry accumulates from 38 to 270 mg/100 g of ellagic acid. In small amounts the cultivated red raspberry consists of the following substances: carotenoids (β-carotene – 9.3 mg/100 g, zeaxanthin – 11 mg/100 g, lutein – 320 mg /100 g), vitamin E – 0.15–0.44 mg/100 g tocopherol equivalents, thiamine – 0,020 mg/100 g, riboflavin – 0.034 mg/100 g, niacin – 0.036 mg/100 g, pyridoxin – 0.05 mg/100 g, biotin – 5.7 mg/100 g. Total content of antioxidants in the cultivated raspberry fruit is 1.71 mg/g (standard quercetin). As a result of the analysis of different literary sources the author determined that raspberry antioxidant properties are mainly connected with high polyphenol content. Contribution of vitamin C into total antioxidant activity is relatively low. Though raspberry fruit chemical composition has already been studied quite well it is necessary to perform further more detailed research of different raspberry cultivars considering their total antioxidant activity as well as certain biochemical components which comprise antioxidant complex of raspberry fruit. The obtained results will form the base for further research aimed at investigating phytochemical compounds of fruit crops which have become an essential part of healthy human diet and developing nutraceutical products.

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