Effect of the total fraction of Bacillus subtilis GM5 lipopeptides on the growth parameters and formation of the bacterial microbiota of broiler chickens

Intestinal microbes play a key role in the energy metabolism of broiler chickens, participate in the development of the gastrointestinal tract, including the regulation of intestinal epithelial proliferation, vitamin synthesis and ion absorption, fermentation of carbohydrates and proteins, biotransformation of bile acids, protection from pathogens and modulation of the immune system. Metagenomic analysis of the gastrointestinal microbiota allows to find approaches to improve the growth and productivity of chickens by introducing a diet based on beneficial bacterial strains or their secondary metabolites. In this paper, we studied the effect of the total fraction of Bacillus subtilis GM5 lipopeptides on the growth parameters and formation of bacterial communities in the caecum of cross Cobb 500 broiler chickens. It was found that the addition of bacillary lipopeptides to the feed resulted in an increase in chicken weight by 12.7% and a decrease in feed conversion by 6.36% compared to the control (P < 0.05). It was also shown that the introduction of a feed additive in the form of a lipopeptide fraction modulates the structure of the bacterial microbiota of the caecum of chickens. Thus, the proportion of classes Bacteroidia, Negativicutes, Betaproteobacteria, Epsilonproteobacteria, Deltaproteobacteria, Synergistia in the caecal microbiota of chickens of the experimental group increases, and the proportion of Clostridia, Methanobacteria decreases in comparison with the control.

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The Effect of Dietary Halloysite Supplementation on the Performance of Broiler Chickens and Broiler House Environmental Parameters

Animals ◽

10.3390/ani11072040 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2040

Author(s):

Małgorzata Nadziakiewicz ◽

Marcin Wojciech Lis ◽

Piotr Micek

Keyword(s):

Body Weight ◽

Broiler Chickens ◽

Body Weight Gain ◽

Environmental Parameters ◽

Feed Consumption ◽

Feed Additive ◽

Feed Conversion ◽

Daily Body Weight ◽

Daily Water ◽

Broiler House

The aim of the study was to determine the effect of supplementing broiler chickens’ diets with halloysite on daily body weight gain (BWG), feed conversion ratio (FCR), daily water consumption (DWC), and some broiler house hygiene parameters. The trial was conducted on 18,000 broiler chickens divided into two groups throughout the 42-day (D) rearing period. The birds were fed complete diets without (group C) or with halloysite addition (1%, group E) from D8 of rearing. No difference in the mortality rate was observed between groups C and E. Birds from group E had a tendency (0.05 < p < 0.10) towards a higher body weight at D32 and D42, a higher BWG, and a lower FCR compared to group C during the entire rearing period. Average DWC differed only in the finisher period, with a tendency towards lower overall DWC in group E. The concentration of ammonia in the air from D21 to D35 was increased more than 5-fold in group C but only 1.5-fold in group E. In conclusion, the use of halloysite as a feed additive in the diet of broiler chickens resulted in a reduction in feed consumption per unit of BWG and higher utilisation of crude protein, which led to improved environmental conditions.

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Dietary Grape (Vitis vinifera) Seed Powder and Zn–Gly Chelate Complex for Mitigating Heat Stress in Broiler Chickens: Growth Parameters, Malondialdehyde, Paraoxonase-1, and Antibody Titer

Agriculture ◽

10.3390/agriculture11111087 ◽

2021 ◽

Vol 11 (11) ◽

pp. 1087

Author(s):

Muhammad Israr ◽

Naila Chand ◽

Rifat Ullah Khan ◽

Ibrahim A. Alhidary ◽

Mutassim M. Abdelrahman ◽

...

Keyword(s):

Heat Stress ◽

Antibody Titer ◽

Broiler Chickens ◽

Growth Traits ◽

Growth Parameters ◽

Control Diet ◽

Paraoxonase 1 ◽

Control Treatment ◽

Broiler Chicks ◽

Feed Conversion

A total of 300 day old broiler chicks (Hubbard) were assigned to 30 floor pens (10 birds per pen) under cyclic heat stress. Three diets including a control, as well as two levels of grape seed powder (GSP) and zinc (OZ) at the rates of 2.5 g/kg GSP + 50 mg/kg OZ and 5 g/kg GSP + 50 mg/kg OZ, were supplied to the broilers for 35 days. According to the results, broiler feed intake improved (p < 0.05) in GSP + OZ groups from 3–5 weeks and on an overall basis compared to the control diet. Body weight increased (p < 0.05) in GSP-5 + OZ-50 during weeks 2–5 and on an overall basis. The findings indicated that feed conversion ratio (FCR) decreased (p < 0.05) during week 5 in broilers supplemented with GSP-5 + OZ-50. The antibody titer (HI) against Newcastle disease (ND) was higher (p < 0.05) in GSP + OZ groups compared to control treatment. The value of malondialdehyde (MDA) decreased (p < 0.05) under GSP + OZ diets compared to control. Moreover, paraoxonase (PON1) was higher (p < 0.05) in GSP + OZ groups compared to untreated broilers. In conclusion, GSP + OZ positively supported growth traits, reduced MDA, and augmented PON1 and HI titer against ND in broilers exposed to heat stress.

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Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

Microorganisms ◽

10.3390/microorganisms7030071 ◽

2019 ◽

Vol 7 (3) ◽

pp. 71 ◽

Cited By ~ 9

Author(s):

Cristiano Bortoluzzi ◽

Bruno Serpa Vieira ◽

Juliano Cesar de Paula Dorigam ◽

Anita Menconi ◽

Adebayo Sokale ◽

...

Keyword(s):

Bacillus Subtilis ◽

Clostridium Perfringens ◽

Intestinal Microbiota ◽

Broiler Chickens ◽

Body Weight Gain ◽

Dietary Supplementation ◽

Necrotic Enteritis ◽

Feed Conversion ◽

Negative Effects ◽

Infected Control

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

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The effect of using fermented lime (Citrus aurantifolia) waste powder (FLWP) on performance, lymphoid organs, and heterophil/lymphocyte ratio of broiler chickens

Livestock and Animal Research ◽

10.20961/lar.v19i3.50932 ◽

2021 ◽

Vol 19 (3) ◽

pp. 282

Author(s):

Hanna Dzawish Shihah ◽

Dwi Sunarti ◽

Sri Sumarsih

Keyword(s):

Broiler Chickens ◽

Relative Weight ◽

Abdominal Fat ◽

Bursa Of Fabricius ◽

Feed Additive ◽

Feed Conversion ◽

Fat Percentage ◽

Final Body Weight ◽

Lymphocyte Ratio ◽

Body Resistance

Objective: Fermented lime waste had potential value as a feed additive for poultry. A Bioactive compound such as citric acid in the lime waste can improve the broiler's performance and body resistance. The study purposed to evaluate the effect of using fermented lime waste powder (FLWP) on the broiler performance and body resistance.Methods: This study used 200 female broiler strain Lohman MB 202 chickens. The research design used a completely randomized design with four treatment levels of fermented lime waste powder (FLWP) by 0%, 1%, 2%, and 3% in every rations with each treatment replicated five times. The parameters observed are feed intake, feed conversion ratio, final body weight, carcass percentage, abdominal fat percentage, lymphoid organ’s relative weight, and heterophil/lymphocyte ratio (H/L ratio). Data were calculated using the analysis of variance and difference test with Duncan's Multiple Range Test. Data analysis were calculated using the SPSS 19.0 program.Results: The result indicates that using FLWP improved (p<0.05) the relative weight of the Bursa of fabricius at T0 0.11%; T1 0.19%; T2 0.19%; and T3 0.17% respectively. The FLWP at 3% level improved (p<0.05) broiler chicken’s H/L ratio. Performance, carcass percentage, and abdominal fat percentage among treatments were not influenced (p>0.05) by FLWP addition.Conclusions: It concludes that adding FLWP at a 1% level increases the relative weight of Bursa of fabricius (0.17-0.19%) without depressed performance of broiler.

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Effect of probiotic strains of Bacillus subtilis on the growth parameters of broiler chickens and caecal microbiota

E3S Web of Conferences ◽

10.1051/e3sconf/202022202054 ◽

2020 ◽

Vol 222 ◽

pp. 02054

Author(s):

Ayslu Mardanova ◽

Anastasia Nikolaeva ◽

Guzel Lutfullina ◽

Aygul Ganeeva ◽

Margarita Sharipova

Keyword(s):

Bacillus Subtilis ◽

Broiler Chickens ◽

Pathogenic Bacteria ◽

Growth Parameters ◽

Live Weight ◽

Control Group ◽

Probiotic Properties ◽

Probiotic Strains ◽

Opportunistic Pathogenic ◽

Caecal Microbiota

In this paper, the probiotic properties of Bacillus subtilis GM2 and GM5 strains were studied. It is shown that the use of probiotic additives based on the spores of these bacteria leads to an increase in the live weight gain of broiler chickens by 4.16% and 10.76% relative to the control. Metagenomic analysis showed that representatives of the phylum Firmicutes (54.55%) and Bacteroidetes (30.45%), mainly represented by the families Ruminococcacea and Bacteroidaceae, predominate in the caecal microbiota of broiler chickens on day 42. It was found that a probiotic based on the B. subtilis GM5 strain leads to an increase in the proportion of Firmicutes in caecum by 27% and a decrease in Bacteroidetes by 19%. There was also a significant decrease in the number of representatives of opportunistic pathogenic bacteria of the Enterobacteriaceae family relative to the control group.

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Effect of dietary garlic and thyme seed supplementation on the production performance, carcass yield and gut microbial population of broiler chickens

Journal of Scientific Agriculture ◽

10.25081/jsa.2017.v1.872 ◽

1970 ◽

Vol 1 ◽

pp. 269

Author(s):

Phyo Htet Htet Kyaw, Kyaw San Win, Khin Khin Lay ◽

Kyaw Kyaw Moe ◽

Aye Aye Maw, Khin Hnin Swe

Keyword(s):

Broiler Chickens ◽

Microbial Population ◽

Control Diet ◽

Production Performance ◽

Feed Additive ◽

Feed Conversion ◽

Carcass Yield ◽

Garlic Powder ◽

Significant Difference ◽

Dietary Treatments

A total of 200 Cobb 500 male broilers were randomly allocated to 4 treatments consisting of 5 replications with 10 chicks each in order to evaluate the effect of dietary garlic and thyme seed supplementation on the production performance, carcass yield and gut microbial population. Treatments were control diet (T1), control diet with 1% thyme seed powder (T2), control diet with 1% garlic powder (T3) and control diet with 0.5% thyme seed and 0.5% garlic powder (T4). Feed intake, body weight, weight gain, feed conversion ratio (FCR), carcass yield was not significantly (p>0.05) improved by dietary treatments compared to control. Escherichia coli (E. coli) count in the gut of broilers did not show significant difference among dietary treatments. However, Lactobacilli count in the gut of broilers significantly (p<0.05) increased in T2 compared to that of T1. Lactobacilli count in the gut of broilers received T1, T3 and T4 did not differ significantly (p>0.05) with each other. It was concluded that thyme seed was reliable as feed additive in the broiler diet and could provide positive advantages to the colonization and proliferation of Lactobacilli.

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Dietary Resin Acid Concentrate Improved Performance of Broiler Chickens and Litter Quality in Three Experiments

Animals ◽

10.3390/ani11113045 ◽

2021 ◽

Vol 11 (11) ◽

pp. 3045

Author(s):

Krzysztof Lipiński ◽

Juhani Vuorenmaa ◽

Magdalena Mazur-Kuśnirek ◽

Katarzyna Sartowska-Żygowska ◽

Hannele Kettunen

Keyword(s):

Broiler Chickens ◽

Litter Quality ◽

Resin Acid ◽

Efficiency Index ◽

Resin Acids ◽

Feed Consumption ◽

Feed Additive ◽

Feed Conversion ◽

Broiler Performance ◽

Improved Performance

Dietary coniferous resin acids have previously been suggested to support the intestinal integrity of broiler chickens by reducing mucosal collagen degradation. The present study examined the effects of resin acid concentrate (RAC) on broiler performance and litter quality. In trial 1, RAC was added to diets at 0, 125, 250, or 1250 g/ton, while in trials 2 and 3, RAC dosing was 0 or 175 g/ton. Bird weight, feed consumption, mortality, feed conversion ratio (FCR), European Efficiency Index (EEI), litter moisture, and footpad dermatitis (FPD) lesions were measured. In trial 1, RAC at 125 and 250 g/ton improved weight gain and EEI, while RAC at 1250 g/ton group did not differ from control. Feed consumption, FCR, FPD scores and mortality were similar in all treatments, but litter quality was improved by all doses of RAC. In trials 2 and 3, RAC increased the final weight of birds, improved FCR, EEI, and litter quality, but had no effects in other parameters. In summary, RAC at 125–250 g/ton improved bird performance and thus shows promise as a feed additive. The dryer litter in RAC treatments may suggest improved intestinal condition as a response to in-feed resin acids.

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Preliminary Study on the Effect of Bacillus amyloliquefaciens TL on Cecal Bacterial Community Structure of Broiler Chickens

BioMed Research International ◽

10.1155/2019/5431354 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

Yuxuan Hong ◽

Yingxian Cheng ◽

Yanjuan Li ◽

Xiaowen Li ◽

Zutao Zhou ◽

...

Keyword(s):

Growth Performance ◽

Bacillus Amyloliquefaciens ◽

Broiler Chickens ◽

High Throughput Sequencing ◽

Control Diet ◽

Probiotic Strain ◽

Feed Additive ◽

Control Group ◽

Feed Conversion ◽

Cecal Microbiota

Probiotics can promote the health and growth performance of animals through modulation of intestinal microbiota. When used as a feed additive, they have the potential to minimize or abolish the use of antibiotics. In this study, we investigated the effect of the probiotic strain Bacillus amyloliquefaciens TL on the growth performance and cecum microflora composition in Cobb 500 broiler chickens. In total, 180 broilers were randomly divided into three groups—each group comprised 4 pens, and each pen contained 15 chickens. The three groups were fed either a control diet, or a diet supplemented with either the antibiotic chlortetracycline or B. amyloliquefaciens TL. Broilers were weighed, and cecum contents were collected on days 7, 14, 21, and 35, respectively. The broilers in both the antibiotic and probiotic groups exhibited significant weight gain compared with controls, exhibiting increases of 16.02% and 13.40%, respectively, after 35 days (P<0.01). Similarly, the feed conversion ratio (FCR, 1–35 days) of broilers in the chlortetracycline and B. amyloliquefaciens TL groups was lower than that of the controls. HiSeq high-throughput sequencing of 16S rRNA of the cecal microbiota was performed on days 7, 14, 21, and 35, respectively. The Firmicutes/Bacteroidetes ratio was higher in the chlortetracycline and B. amyloliquefaciens TL groups than in the control group on days 14, 21, and 35, and especially on day 21. The prevalence of genera Oscillospira, Ruminococcus, Butyricicoccus, and Faecalibacterium (Firmicutes) was higher in the antibiotic and probiotic groups, while that of Bacteroides, Parabacteroides (Bacteroidetes), and Lactobacillus was higher in the control group. In this study, the changes in the microbiota of the probiotic group were similar to those in the antibiotic group. These results suggest that the probiotic strain B. amyloliquefaciens TL can modulate the cecal microbiota of broilers similar to chlortetracycline.

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Evaluation of the supplementation of a feed additive as a potential protector against the adverse effects of 2.5 ppm T-2 toxin on growing broiler chickens

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-8416 ◽

2016 ◽

Vol 68 (3) ◽

pp. 709-715 ◽

Cited By ~ 4

Author(s):

G.J. Diaz ◽

M.L. Vargas ◽

A. Cortés

Keyword(s):

Adverse Effects ◽

Broiler Chickens ◽

Digestive System ◽

Body Weight Gain ◽

Relative Weight ◽

Experimental Treatment ◽

Additive Group ◽

Negative Control ◽

Feed Additive ◽

Feed Conversion

ABSTRACT A trial was conducted to evaluate a feed additive containing epoxidase activity from a bacterium (Mycofix-S) as a potential protection against the adverse effects of 2.5 ppm dietary T-2 toxin in male growing broiler chickens. A total of 144 one-day-old Ross 308 male chicks were individually wing-banded and allotted into each of the four experimental groups. Group 1: negative control, no T-2 toxin or additive; group 2: Mycofix-S, 2.5 g/kg; group 3: positive control, 2.5 ppm T-2 toxin; group 4: 2.5 ppm T-2 toxin + 2.5 g/kg Mycofix-S. Feed and water were provided ad libitum for 28 days (days 1 to 28 of age). Each experimental treatment was replicated 6 times, with 6 birds per replicate pen. Response variables included performance parameters, serum activity of alkaline phosphatase (ALP) and amylase, relative weight of selected organs and histology of the upper digestive system. T-2 toxin at 2.5 ppm significantly (P = 0.016) decreased the 28-day body weight gain and cumulative feed intake without affecting feed conversion. The feed additive counteracted these adverse effects. Serum enzyme activities were not significantly (P>0.05) affected for the four experimental groups but when data from the groups receiving T-2 toxin was pooled and compared against the pooled data from groups without the toxin a significant decrease in amylase activity was observed in chickens receiving T-2 toxin. The histological examination of the upper digestive system revealed lesions in mouth, esophagus, proventriculus, gizzard and duodenum in the chickens fed T-2 toxin without the additive. Chickens fed T-2 toxin plus the additive showed lesions in the same tissues except in the duodenum. The results of the present study show that the addition of 2.5 g/kg of the feed additive tested protects against adverse effects on performance and also the integrity of the duodenal mucosa.

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Phytogenic additive as an alternative to growth promoters in broiler chickens

Ciência Rural ◽

10.1590/s0103-84782009000200032 ◽

2009 ◽

Vol 39 (2) ◽

pp. 522-527 ◽

Cited By ~ 10

Author(s):

Gerson Neudí Scheuermann ◽

Anildo Cunha Junior ◽

Lucas Cypriano ◽

Alexandre Mossate Gabbi

Keyword(s):

Body Weight ◽

Broiler Chickens ◽

Positive Control ◽

Negative Control ◽

Feed Additive ◽

Broiler Chicks ◽

Growth Promoter ◽

Feed Conversion ◽

Growth Promoters ◽

The Cost

This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler chicks were distributed into four treatments: negative control (without growth promoter); positive control (avilamycine, 10ppm + colistin, 15ppm); and two alternative treatments with 150ppm of phytogenic additive, one with a reduced Ca and P levels diet (PA-R1) and the other with lower energy, and amino acids, besides Ca and P (PA-R2). The trial was conducted with 12 replicates, each consisted of a pen with 34 birds. The alternative diets showed body weight intermediate to the two controls at 42 days, with no significant (P>0.05) treatment effect on feed conversion ratio. No treatment differences (P>0.05) on carcass yield and composition was observed. There was a tendency of abdominal fat lipids saturation, when the phytogenic additive was used, as possible consequence of a decreased level of soybean oil in the diets. A difference (P<0.001) on ingredient consumption profile was observed between the treatments. All together, this study showed a possibility to reduce the cost of total feed used to produce a broilers or a ton of body weight by the utilization of the tested phytogenic additive.

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