Effect of storage on retinol content and total antioxidant capacity of human milk

2019 ◽  
Vol 122 (2) ◽  
pp. 606-616 ◽  
Author(s):  
Jaísa Oliveira Chaves ◽  
Angelica Maria de Freitas Fernandes ◽  
Paola Machado Parreiras ◽  
Gustavo Silveira Breguez ◽  
Maria Cristina Passos ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Human Milk ◽  
Aluminum Foil ◽  
Storage Conditions ◽  
Content Type ◽  
Total Antioxidant ◽  
Effects Of Temperature ◽  
Freezing Temperatures ◽  
Milk Banks ◽  
The Impact

Purpose The purpose of this paper is to evaluate the effect of different times and freezing temperatures on the antioxidant activity of raw human milk (HM) and the impact of light by different packaging on retinol level and the antioxidant activity of pasteurized HM. Design/methodology/approach Donor milks were homogenized to form the pool of the experimental study characterized by the evaluation of the effects of time (0, 2, 4, 8 and 15 days) freezing temperatures (−3°C, −8°C and −18°C) and the interference of the type of packaging on the antioxidant activity and retinol levels of HM. Findings The existing studies do not reveal the real impact of HM storage conditions adopted by human milk banks (HMB) in Brazil on their compounds, mainly in relation to the effects of temperature and freezing time and the incidence of light on retinol levels and antioxidant activity. In view of the already documented importance of these compounds for the growth, development and health of children, it is extremely important to assess their stability according to the procedures adopted by the banks. It has been observed in this study that lower freezing temperatures (−18°C) further preserve the antioxidant activity. It was found that the amber and transparent vials wrapped with aluminum foil allowed for greater retinol stability of HM, with values of 2.501±0.757 µmol/L and 4.991±0.825 µmol/L, respectively. On the contrary, there was no significant influence on antioxidant activity. Originality/value It is suggested that HMB store milk at lower temperatures and use glass jars that block the passage of light.

The Impact of Storage Conditions on the Stability of Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis Bb12 in Human Milk

2017 ◽  
Vol 12 (9) ◽  
pp. 566-569 ◽  
Author(s):  
Anastasia Mantziari ◽  
Juhani Aakko ◽  
Himanshu Kumar ◽  
Satu Tölkkö ◽  
Elloise du Toit ◽  
...  
Keyword(s):  
Human Milk ◽  
Lactobacillus Rhamnosus ◽  
Storage Conditions ◽  
Lactobacillus Rhamnosus Gg ◽  
Bifidobacterium Animalis ◽  
The Stability ◽  
The Impact

scholarly journals Storage study of cereal bars formulated with banana peel flour

2018 ◽  
Vol 48 (3) ◽  
pp. 386-396 ◽  
Author(s):  
Vania Silva Carvalho ◽  
Ana Carolina Conti-Silva
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Storage Period ◽  
Banana Peel ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Total Phenolic Compounds ◽  
Content Type ◽  
Total Antioxidant ◽  
Cereal Bars

Purpose This paper aims to examine the bioactive compounds and texture properties of cereal bars formulated with banana peel flour during storage. Design/methodology/approach Seven cereal bars were produced and stored during 11 months, under vacuum and protected from the light. The total phenolic compounds, the activity antioxidant by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method, the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) method expressed as EC50 (concentration required to reduce the original amount of free radicals by 50 per cent) and texture properties were evaluated over the storage period. Findings In general, total phenolic compounds decreased during storage (from 4.19 to 1.11 mg GAE. g-1f.w.). Although the total antioxidant activity (ABTS method) increased during the fifth month, it reduced during storage (from 3.41 to 0.30 µmol TE.g-1f.w.); and the EC50 was not modified in many formulations, though it decreased in other formulations during storage period (from 3913 to 19221 g fruit.g-1 DPPH). The force of rupture began to increase in the fourth month (reaching 62.4 N), and hardness began to increase in the ninth month (reaching 444 N). The formulation and time factors influenced the total phenolic compounds, total antioxidant activity (ABTS method), force of rupture and hardness, while EC50 was only influenced by the formulation (p-value = 0.001). A principal component analysis showed that time had little effect on the most important characteristics considered in description of the cereal bars. Originality/value Cereal bars can be consumed up to the third month of storage, considering the texture of the products. Moreover, the presence of bioactive compounds in cereal bars depends on the addition of banana peel flour, which it contributes to the insertion of total phenolic compounds and total antioxidant activity in cereal bars, aggregating functional properties in these products.

scholarly journals Effect of Different Cooking Methods on Polyphenols, Carotenoids and Antioxidant Activities of Selected Edible Leaves

Antioxidants ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 117 ◽  
Author(s):  
K. Gunathilake ◽  
K. Ranaweera ◽  
H. Rupasinghe
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Leafy Vegetables ◽  
Cooking Methods ◽  
Oxygen Scavenging ◽  
Total Antioxidant ◽  
The Impact ◽  
Dpph Radical Scavenging

This study aimed to evaluate the effect of cooking (boiling, steaming, and frying) on polyphenols, flavonoids, carotenoids and antioxidant activity of six edible leaves. The total antioxidant capacity of the fresh and cooked leaves was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and singlet oxygen scavenging assays. The results revealed that frying caused a reduction in major bioactives and antioxidant activities in all leafy vegetables tested. However, steamed and boiled leaves of C. auriculata and C. asiatica have shown greater levels of polyphenols, flavonoids, and antioxidant capacity compared with fresh leaves. Polyphenol and flavonoid contents of boiled S. grandiflora and G. lactiferum were higher than that of their fresh form. Boiled and steamed O. zeylanica and S. grandiflora have shown higher carotenoids. Boiled and steamed leaves of P. edulis have shown higher antioxidant activity. The impact of cooking on the changes in bioactive concentrations and antioxidant capacities are dependent on the species and the method of cooking.

scholarly journals Consumption of Lysozyme-Rich Milk Can Alter Microbial Fecal Populations

2012 ◽  
Vol 78 (17) ◽  
pp. 6153-6160 ◽  
Author(s):  
Elizabeth A. Maga ◽  
Prerak T. Desai ◽  
Bart C. Weimer ◽  
Nguyet Dao ◽  
Dietmar Kültz ◽  
...  
Keyword(s):  
Human Milk ◽  
Gut Microbiome ◽  
16S Rrna Gene Sequencing ◽  
Fecal Microbiota ◽  
Rrna Gene ◽  
Gut Health ◽  
Content Type ◽  
Microbiome Composition ◽  
The Impact ◽  
Over Time

ABSTRACTHuman milk contains antimicrobial factors such as lysozyme and lactoferrin that are thought to contribute to the development of an intestinal microbiota beneficial to host health. However, these factors are lacking in the milk of dairy animals. Here we report the establishment of an animal model to allow the dissection of the role of milk components in gut microbiota modulation and subsequent changes in overall and intestinal health. Using milk from transgenic goats expressing human lysozyme at 68%, the level found in human milk and young pigs as feeding subjects, the fecal microbiota was analyzed over time using 16S rRNA gene sequencing and the G2 Phylochip. The two methods yielded similar results, with the G2 Phylochip giving more comprehensive information by detecting more OTUs. Total community populations remained similar within the feeding groups, and community member diversity was changed significantly upon consumption of lysozyme milk. Levels ofFirmicutes(Clostridia) declined whereas those ofBacteroidetesincreased over time in response to the consumption of lysozyme-rich milk. The proportions of these major phyla were significantly different (P< 0.05) from the proportions seen with control-fed animals after 14 days of feeding. Within phyla, the abundance of bacteria associated with gut health (BifidobacteriaceaeandLactobacillaceae) increased and the abundance of those associated with disease (Mycobacteriaceae,Streptococcaceae,Campylobacterales) decreased with consumption of lysozyme milk. This study demonstrated that a single component of the diet with bioactivity changed the gut microbiome composition. Additionally, this model enabled the direct examination of the impact of lysozyme on beneficial microbe enrichment versus detrimental microbe reduction in the gut microbiome community.

scholarly journals Antioxidant Activity and Volatile Composition of Red Araçá Pulp Under Different Drying Conditions

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Michele C. M. Bombardelli ◽  
Gracielle Johann ◽  
Christiane S. Machado ◽  
Yohandra R. Torres ◽  
Fernando Palú ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Moisture Diffusivity ◽  
Total Antioxidant Activity ◽  
Volatile Composition ◽  
Fruit Extracts ◽  
Total Antioxidant ◽  
Drying Conditions ◽  
The Impact

Araçá fruit extracts were dried at different air conditions, and an investigation of the impact of drying on the volatile composition and antioxidant activity of araçá extracts was conducted. The effective moisture diffusivity varied between 8.542 × 10−8 and 13.34 × 10−8 m2/min. Fruit extracts dried at 50°C and 2.0 m/s had the highest total antioxidant activity (1916.10 mgascorbic acid/100 garaçá). The highest phenolic content (556.28 mgGAE/100 garaçá) was obtained when fruits were dried at 40°C and 1.5 m/s, but the resulting extract contained high amounts of 5-hydroxymethylfurfural (HMF), a contaminant formed in sugar-rich foods as a result of heating. Araçá extracts had similar qualitative profiles of volatile compounds by GC-MS, with caryophyllene being the most abundant terpene, followed by 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, selina-3,7(11)-diene, γ-terpinene, γ-cadinene, and α-salinene. HMF corresponded to the major peak in all chromatograms, proving that thermal drying affected the quality of the extracts.

scholarly journals Total antioxidant activity and trace elements in human milk: the first 4 months of breast-feeding

2009 ◽  
Vol 230 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Carla Matos ◽  
Carla Moutinho ◽  
Victor Balcão ◽  
Cristina Almeida ◽  
Marta Ribeiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Trace Elements ◽  
Human Milk ◽  
Breast Feeding ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

scholarly journals Effect of Different Forms of Sulfur Fertilization on Bioactive Components and Antioxidant Activity of White Cabbage (Brassica Oleracea L.)

2021 ◽  
Vol 11 (18) ◽  
pp. 8784
Author(s):  
Agnieszka Nawirska-Olszańska ◽  
Anita Biesiada ◽  
Agnieszka Kita
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Storage Conditions ◽  
Potassium Sulfate ◽  
Bioactive Components ◽  
White Cabbage ◽  
The Impact ◽  
Sulfur Fertilization

Cruciferous vegetables are very popular in latitudes corresponding to central and eastern Europe. They are rich in bioactive compounds such as chlorophylls, carotenoids, and polyphenols. The type and quality of fertilization has a significant impact on their chemical composition. The aim of this study was to determine the impact of specific forms of sulfur fertilization on the chemical properties of white cabbage, and to explore the effect of storage conditions on its bioactive components and antioxidant activity. The research material was the late cultivar of white cabbage ‘Stonehead’, fertilized with a dose of 30 kg S·ha−1, administered in the form of elemental sulfur, ammonium sulfate, and potassium sulfate. Sulfur fertilization had a significant impact on the parameters selected for this study, and the form in which it was applied resulted in different effects on these individual parameters. For all parameters investigated, the effect of sulfur fertilization was detectable. Antioxidant properties, determined as both ABTS and DPPH radical-scavenging activities, were the highest in cabbage harvested from the plot treated with potassium sulfate

scholarly journals The Impact of Human Milk on Necrotizing Enterocolitis: A Systematic Review and Meta-Analysis

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1322 ◽  
Author(s):  
Emma Altobelli ◽  
Paolo Matteo Angeletti ◽  
Alberto Verrotti ◽  
Reimondo Petrocelli
Keyword(s):  
Systematic Review ◽  
Human Milk ◽  
Risk Reduction ◽  
Necrotizing Enterocolitis ◽  
Premature Infants ◽  
Meta Analysis ◽  
Preterm Formula ◽  
The World ◽  
Milk Banks ◽  
The Impact

Background. Premature infants receiving breastfeed have a lower incidence of NEC than those fed preterm formula. This study aimed: (1) to update a systematic review and meta-analyses to evaluate the relationship between feeding and necrotizing enterocolitis (NEC) in low weight premature infants; (2) to conduct meta-regression analyses by subgroups; (3) to describe geographical distribution of milk banks in the world. Methods. Papers included in the meta-analysis were updated as of June 2019. Relative risks were used as a measure of effect size. Random effect models were used to account for different sources of variation among studies. For milk banks, the data reviewed by the literature were integrated with the information collected from countries’ institutional sites and milk bank networks. Results. Thirty-two papers were included in meta-analysis: six randomized controlled trials (RCTs) and 26 observational studies (OS). The census has found 572 milk banks around in the world. Brazil has the most active milk banks. RCTs meta-analysis indicates a risk reduction of NEC using human milk respect to formula: Relative risk (RR) = 0.62 (0.42–0.93). Seven OS compared quantities lower than human milk or higher than the 50th quantile showing a risk reduction of NEC:RR = 0.51 (0.31–0.85); 3 OS that evaluated human milk versus mixed feeding showing that human milk has a protective role on the development of NEC:RR = 0.74 (0.63–0.91). Results of subgroups analysis show that the risk reduction is statistically significant only for studies in which premature infants are given both their own and donated breastmilk. Conclusions. The possibility of preserving human milk and promoting donations guarantees an improvement in the health of newborns.

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