scholarly journals Antibacterial Activity of Rosmarinus officinalis, Zingiber officinale, Citrus aurantium bergamia, and Copaifera officinalis Alone and in Combination with Calcium Hydroxide against Enterococcus faecalis

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Silmara Silva ◽  
Nayane Alves ◽  
Priscila Silva ◽  
Thalita Vieira ◽  
Panmella Maciel ◽  
...  
Keyword(s):  
Essential Oils ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Rosmarinus Officinalis ◽  
Citrus Aurantium

This study aimed to evaluate the efficacy of different concentrations of essential oils combined with calcium hydroxide against Enterococcus faecalis. Thirteen experimental groups were formed: NC (negative control); PC (positive control); GC (growth control); SC (sterilization control); RO (Rosmarinus officinalis); ROH (calcium hydroxide + RO); ZO (Zingiber officinale); ZOH (calcium hydroxide + ZO); AB (Citrus aurantium bergamia); ABH (calcium hydroxide + AB); CO (Copaifera officinalis); COH (calcium hydroxide + CO); DWH (calcium hydroxide and distilled water). After reconstitution of the E. faecalis strain, microdilution testing was performed to define the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The data were tabulated in an Excel spreadsheet, and the MIC and MBC were calculated in accordance with the Bacteriological Analytical Handbook. MICs in the range of 0 to 100 mg/ml were only observed in the ROH group. The RO, ROH, AB, ZO, and ZOH presented absolute data for MBC. Bacterial growth was detected in the DWH group at all concentrations tested. The combination of the essential oils tested here with calcium hydroxide appears promising as an intracanal medication in endodontic treatment because of its effectiveness against Enterococcus faecalis. Essential oils are important in endodontic therapy since calcium hydroxide, the gold standard intracanal medication, is not effective against E. faecalis.

scholarly journals Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

2013 ◽  
Vol 24 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Roberta Vieira Farac ◽  
Antonio Carlos Pizzolitto ◽  
Juliane Maria Guerreiro Tanomaru ◽  
Renata Dornelles Morgental ◽  
Regina Karla de Pontes Lima ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Ex Vivo ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Human Teeth ◽  
Root Canals ◽  
Colony Forming Units

This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion, among the evaluated medications OZ-PG and CH/CPMC were the most effective against E. faecalis.

scholarly journals Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

2018 ◽  
Vol 36 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nattriya Likitpongpipat ◽  
Somboon Sangmaneedet ◽  
Poramaporn Klanrit ◽  
Rajda Noisombut ◽  
Suttichai Krisanaprakornkit ◽  
...  
Keyword(s):  
Wound Healing ◽  
New Zealand ◽  
Calcium Hydroxide ◽  
Median Number ◽  
Positive Control ◽  
Negative Control ◽  
Histological Evaluation ◽  
Dentinal Tubules ◽  
Pulp Capping ◽  
New Zealand White Rabbits

This study examined and compared wound healing between Thai propolis product and calcium hydroxide paste as pulp-capping agents after partial pulpotomy in New Zealand white rabbits. Forty incisor teeth from 10 rabbits were treated. Thirty-six teeth received class V cavity preparations with partial pulpotomy and application of either propolis or calcium hydroxide paste. Similar cavity preparations were performed in 2 teeth without any capping material as a positive control, whereas 2 teeth without the cavity preparation served as a negative control. Histological evaluation showed that both groups had dentin bridge formation. Dentinal tubules in the dentin bridge were more orderly arranged in the Thai propolis group than in the calcium hydroxide group. Wound healing and the median number of hyperemic blood vessels were not statistically significant different between the 2 groups. Thai propolis product may be used as a pulp-capping agent.

scholarly journals Essential oils as tick repellents on clothing

2019 ◽  
Vol 79 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Oliver Soutar ◽  
Freya Cohen ◽  
Richard Wall
Keyword(s):  
Essential Oils ◽  
No Reduction ◽  
Positive Control ◽  
Negative Control ◽  
Tick Abundance ◽  
Spearmint Oil ◽  
Oregano Oil ◽  
Negative Controls ◽  
Tick Repellents

Abstract Essential oils show promise as natural alternatives to synthetic tick repellents, but few studies have investigated their repellent efficacy in vivo or under field conditions. Here, blanket-drags and standardised walks were employed to evaluate tick acquisition by 1 m2 cotton blankets or cotton trousers, respectively, in woodland edge habitats of known high tick abundance. Blankets and trousers had been treated with one of 5% oregano, rosemary, spearmint or thyme oils, 20% DEET (N,N-diethyl-3-methylbenzamide) (positive control) or ethanol excipient-only (negative control). The number of ticks present on the blankets or trousers differed significantly between treatments: spearmint oil treatments resulted in significantly fewer ticks than the negative controls for both blankets and trousers and significantly fewer ticks were present on the oregano oil treated blankets. For ticks that did attach to the trousers, the rate of drop off within 3 min was significantly higher for trousers treated with spearmint oil or thyme oil than ethanol, oregano oil and rosemary oil. No reduction in repellence was detected over a 24 h period between treatment and testing. The results suggest that 5% oregano and spearmint oils exhibit potential as natural clothing repellents, with an effective equivalence to 20% DEET.

scholarly journals Complex Transcriptional Regulatory Mechanisms Control Expression of the E2F3 Locus

2000 ◽  
Vol 20 (10) ◽  
pp. 3633-3639 ◽  
Author(s):  
Monique R. Adams ◽  
Rosalie Sears ◽  
Faison Nuckolls ◽  
Gustavo Leone ◽  
Joseph R. Nevins
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Binding Sites ◽  
Critical Role ◽  
Growth Control ◽  
Constitutive Expression ◽  
Growth Stimulation ◽  
Positive Control ◽  
Negative Control ◽  
Transcriptional Regulatory Mechanisms

ABSTRACT E2F transcription activity has been shown to play a critical role in cell growth control, regulating the expression of a variety of genes that encode proteins important for the initiation of DNA replication and cell cycle regulation. We have shown that the E2F3 locus encodes two protein products: the E2F3a product, which is tightly regulated by cell growth, and the E2F3b product, which is constitutively expressed throughout the cell cycle. To further explore the mechanism controlling the expression of the two E2F3 gene products, we analyzed the genomic sequences flanking the 5′ region of E2F3a and E2F3b. We find that a series of E2F binding sites confer negative control on the E2F3a promoter in quiescent cells, similar to the control of the E2F1 and E2F2 promoters. In addition, a group of E-box elements, which are Myc binding sites, confer responsiveness to Myc and are necessary for full activation of the E2F3a promoter in response to growth stimulation. Based on these results and past experiments, it appears that the E2F1, E2F2, and E2F3a genes are similarly regulated by growth stimulation, involving a combination of E2F-dependent negative control and Myc-mediated positive control. In contrast, the constitutive expression of the E2F3b gene more closely reflects the control of expression of the E2F4 and E2F5 genes.

scholarly journals Effects of avilamycin and essential oils on mRNA expression of apoptotic and inflammatory markers and gut morphology of piglets

2008 ◽  
Vol 53 (No. 9) ◽  
pp. 377-387 ◽  
Author(s):  
A. Kroismayr ◽  
J. Sehm ◽  
M.W. Pfaffl ◽  
K. Schedle ◽  
C. Plitzner ◽  
...  
Keyword(s):  
Essential Oils ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Feed Additives ◽  
Feed Additive ◽  
Pcr Analysis ◽  
Control Group ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples

In this study 120 piglets were allotted to 3 dietary treatments, negative control group, one group receiving a blend of essential oils (EO) derived from oregano, anise and citrus peels (40 mg/kg diet), and a positive control group treated with avilamycin as growth promoting feed additive (40 mg/kg diet). On day 22 of the experiment, 12 representative animals from each treatment group were sacrificed and tissue samples were collected for quantitative real time-PCR analysis and gut tissue morphology. EO and avilamycin decreased the gene expression of the transcriptional factor NFκB and the apoptotic marker TNFα significantly in the ileum and jejunum, respectively. The expression of the proliferation marker Cyclin D1 was also significantly decreased by both substances in the colon, mesenteric lymph nodes and spleen. The colonic crypt depth was reduced by avilamycin, and also numerically by the essential oils. These changes correlated with the up-regulation of the apoptosis factor Caspase 3. Histomorphometry revealed a smaller size of ileal Peyer’s patches through the use of both feed additives, which correlated significantly with lower expression rates of NFκB. In conclusion, the results suggest that EO and avilamycin relieved weaning piglets from an immune defence stress in a similar way.

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS

2020 ◽  
pp. 148-151
Author(s):  
N. Shubhashini ◽  
Vinaya Kumar R ◽  
Annapoorna Kini ◽  
Swetha H.B. ◽  
Srikanth Choudhary B.S. ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Toluidine Blue ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Root Canals

AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue.

scholarly journals Effects of coronal leakage on concentration of hydrogen ions and calcium release of several calcium hydroxide pastes over different periods of time

2013 ◽  
Vol 1 (3) ◽  
pp. 230
Author(s):  
Mariana Pires Crespo ◽  
Milton Carlos Kuga ◽  
Marcus Vinicius Reis Só ◽  
Katia Cristina Keine ◽  
André Luis Shinohara
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Release ◽  
Water Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Filling Material ◽  
Hydrogen Ions ◽  
Ion Release ◽  
Temporary Filling

PURPOSE: To evaluate the effects of coronal leakage on concentration of hydrogen ions (pH) and calcium release of several calcium hydroxide pastes, over different periods of time. MATERIAL AND METHODS:  Fifty extracted human mandibular central incisors (n=10) were instrumented up to the F2 instrument and assigned to the following intracanal dressing: G1- Calen, G2- Calen with 0.4% chlorhexidine (CHX), G3- Calcium hydroxide with camphorated paramonochlorophenol (CPMC) and glycerin, G4- Calen, but temporary filling material maintained during all test (positive control) and G5- Root canal without intracanal dressing (negative control). All groups were immersed in distilled water for 7 days. In sequence, the temporary filling materials were removed, except in controls groups. All specimens were individually mounted on a specific device and only its root again immersed in distilled water. Concentration of hydrogen ions and calcium release by calcium hydroxide pastes in distilled water were evaluated in 24h, 7, 14 and 28 days. The results were submitted to ANOVA test (p = 0.05). After 28 days, root canals from experimental groups were examined in SEM. RESULTS: G1, G2, G3 and G4 presented similar pH values and calcium release and did not differ from each other (p>0.05), up to 7 days. After this time G1, G2 and G3 presented values lower values than G4 (p<0.05). In SEM analysis, calcium hydroxide residues were observed in all experimental groups. CONCLUSIONS: After 7 days, coronal leakage decreased the concentration of hydrogen ions and calcium ion release provided by all calcium hydroxide pastes.

scholarly journals In vitro inhibitory and biofilm disruptive activities of ginger oil against Enterococcus faecalis

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1859
Author(s):  
Shahida Mohd-Said ◽  
Wee Wee Kweh ◽  
Chong Yi Than ◽  
Zamirah Zainal-Abidin ◽  
Siti Noor Adnalizawati Adnan ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Brain Heart Infusion ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Ginger Rhizome ◽  
Culture Suspension ◽  
Dose Dependent ◽  
Concentration Series

Background: This study investigated the antibacterial effect of ginger (Zingiber officinale) oil against a common resistant root canal pathogen known as Enterococcus faecalis. The aim of the study was to determine the inhibition of E. faecalis growth in culture suspension and ability to inhibit growth of bacteria through disruption of pre-formed monospecies biofilm. Methods: Ginger rhizome oil was prepared in two-fold concentration series from 0.04 to 5.00 mg/mL and mixed with brain heart infusion broth inoculated with E. faecalis in anaerobic condition. Among the antibacterial tests performed were the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations using microdilution assays, and anti-biofilm assay on 3-day old pre-form monospecies biofilm on a 94 well-plate. Ampicillin was used as a positive control. Results: The result showed an in vitro dose-dependent bacteriostatic activity towards E. faecalis in suspension broth (MIC 0.04mg/mL) but no bactericidal activity within the tested concentration range. It was also found that the ginger oil inhibitory activity against E. faecalis was comparably less in anti-biofilm activity than against bacteria cultured in suspension solution. Conclusion: The study suggests that at determined concentrations, ginger oil has the potential to be used as an antibacterial agent in the management of root canal infections particularly where newly formed E. faecalis is involved.

scholarly journals Aktivitas Ekstrak Daun Bangle (zingiber purpureum roxb.) Terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa

2020 ◽  
Vol 2 (1) ◽  
pp. 31-38
Author(s):  
Fajrin Noviyanto ◽  
Siti Hodijah ◽  
Yusransyah Yusransyah
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibitory Concentration ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
High Morbidity ◽  
Leaf Extracts ◽  
Control Solution

The bacteria that cause infections that can lead to high morbidity and mortality, the bacterium Pseudomonas aeruginosa. Bangle has a pharmacological activity as antibacterial, laxative, pancreatic lipase inhibitor, and protect cells from damage caused by oxidative stress. The purpose of this study are: to know the chemical constituents present in the extract of leaves bangle (Zingiber purpureum Roxb.) Can be efficacious as an antibacterial and knowing Minimal Inhibitory concentration (MIC) of the extracts of leaves bangle against Pseudomonas aeruginosa. Tests on the leaf extracts for antibacterial activity against Pseudomonas aeruginosa bangle made by the method of Kirby Bauer and solvents used are DMSO. Test solution with a concentration of leaf extract bangle 200, 400, 600, 800 and 1,000 ppm, the positive control solution (ciprofoxacin) and the solution negative control (DMSO). The results showed that the chemical constituents present in the extract of leaves bangle (Zingiber purpureum Roxb.) Are flavonoids, saponins, tannins, alkaloids and steroids. Value Minimum Inhibitory Concentration (MIC) of ethanol extract of the leaf bangle S bacteria Pseudomonas aeruginosa is a concentration of 40 % with an average diameter of 5.44 mm inhibitory. MIC extract ethanol extract of leaf bangle belonging to the bacterial activity that is strong enough..

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