scholarly journals Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF-α-Induced Insulin-Resistant Mouse FL83B Hepatocytes

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Jian-Hua Xu ◽  
Yangming Martin Lo ◽  
Wen-Chang Chang ◽  
Da-Wei Huang ◽  
James Swi-Bea Wu ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Bioactive Compounds ◽  
High Performance ◽  
Liver Steatosis ◽  
Bioactive Compound ◽  
Coumaric Acid ◽  
Hplc Assay ◽  
Resistant Mouse ◽  
Chromatography Column ◽  
Tnf Α

Ruellia tuberosa L. (RTL) has been used as a folk medicine to cure diabetes in Asia. RTL was previously reported to alleviate hyperglycemia, insulin resistance (IR), abnormal hepatic detoxification, and liver steatosis. However, the potential bioactive compounds of RTL have still not been identified. The aim of this study was to investigate the bioactive compounds in RTL ethyl acetate (EA) fractions by using a glucose uptake assay in TNF-α-treated mouse FL83B hepatocytes to discover a mechanism by which to improve IR. The bioactive compounds were identified by the high-performance liquid chromatography (HPLC) assay. Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at the concentration of 25 μg/ml. Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF-α-treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p-coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p-coumaric acid, but its effect alone was not as good as the combined effect of all three compounds of the EAF5-5 fraction. Thus, we speculate that the antidiabetic effect of RTL may be the result of multiple active ingredients.

scholarly journals Screening of Bioactive Compounds from Moutan Cortex and Their Anti-Inflammatory Activities in Rat Synoviocytes

2009 ◽  
Vol 6 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Mengjie Wu ◽  
Zhiyuan Gu
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Ethanol Extract ◽  
Root Bark ◽  
Tnf Α ◽  
Dependent Inhibition ◽  
Moutan Cortex ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Inflammatory Effect

Moutan Cortex, a widely used traditional Chinese medicine for the treatment of various diseases, is the root bark ofPaeonia suffruticosa Andrews(Paeoniaceae). Most of the pharmacological investigations of Moutan Cortex have been addressed to its central nervous system activities, anti-oxidative and sedative actions. Otherwise, there are few reports about the active compounds with anti-inflammatory activity of Moutan Cortex. The aim of the present study was to screen and identify bioactive compounds with anti-inflammatory effect from Moutan Cortex. With the aid of preparative high performance liquid chromatography (HPLC) technique, ethyl acetate and ethanol extract of Moutan Cortex were isolated into twenty-two fractions. Bioactivities of these fractions were evaluated by measuring expression of tumor necrosis factor-α (TNF-α) in rat synoviocytes subjected to interleukin-1β (IL-1β). Eight compounds were isolated from six active fractions and identified by HPLC/MSn. Purified compounds, paeoniflorin, paeonol and pentagalloylglucose resulted in dose-dependent inhibition of TNF-α synthesis and IL-6 production in synoviocytes treated with proinflammatory mediator. These results suggested that paeonol, paeoniflorin, glycosides and pentagalloylglucose contribute to the anti-inflammatory effect of Moutan Cortex.

scholarly journals Comparative Evaluation of Bioactive Compounds and Volatile Profile of White Cabbages

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3696 ◽  
Author(s):  
Ante Lončarić ◽  
Tihana Marček ◽  
Domagoj Šubarić ◽  
Antun Jozinović ◽  
Jurislav Babić ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
High Performance ◽  
Solid Phase ◽  
Sinapic Acid ◽  
Allyl Isothiocyanate ◽  
Bioactive Compound ◽  
Physicochemical Composition ◽  
Dominant Component

Cabbage is an important source of bioactive compound, which is available throughout the year. However, a lot of different traditional, and hybrid varieties with different levels and composition of bioactive compounds can be found on the market. The aim of the study was to obtain quantitative results showing comparative differences between different white cabbages (“Čepinski”, “Varaždinski”, “Bravo”, “Ogulinski”) from Croatia. Morphometric parameters and physicochemical composition were determined while using standard procedures. Phenolic acids were determined using high-performance liquid chromatography and volatile compounds were analysed by the solid-phase micro-extraction gas chromatography with mass spectrometry (SPME-GC-MS) smethod. The results showed that studied cabbage cultivars differed in physicochemical composition and morphological traits. Six phenolic acids were identified and quantified, whereas a sinapic acid was the most dominant component (65.9–78.15 mg/kg). Aldehydes, esters, alcohols, and terpenes were the major classes of organic volatile compounds present in the studied cabbages. “Čepinski”, which has never been analysed before, showed to contain the highest amount of d-limone (40.75 µg/L) and allyl isothiocyanate (1090.26 µg/L), the most important volatile compounds responsible for the fresh cabbage flavour. The presented results mark off “Čepinski” cultivar as valuable for larger production and further examination.

scholarly journals Phytochemical Screening and Quantitative Analysis of Coleus arthropurpureus Ethyl Acetate Fraction and Antibacterial Activity Against Staphylococcus aureus

2020 ◽  
Vol 4 (1) ◽  
pp. 17-23
Author(s):  
Indra Lasmana Tarigan ◽  
Ayu Kumala Sari ◽  
Choirul Huda ◽  
Czellicya Jovanncha ◽  
Afidatul Muadifah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Quantitative Analysis ◽  
Bioactive Compounds ◽  
Retention Time ◽  
High Performance ◽  
Bioactive Compound ◽  
Cell System ◽  
Diffusion Method ◽  
Qualitative And Quantitative

Staphylococcus aureus (S. aureus) is a pathogenic microbe that is caused by various diseases in humans and animals. Infectious diseases caused by S. aureus in Asia reached 70% in 2007, while in Indonesia reached 23.5%. The plant provided several bioactive compounds that might function as an antibacterial which inhibits both bacterial growth and damaging the cell system and protein synthesis. Coleus arthropurpureus known contains alkaloids and tannins that supposed to be an antibacterial compound. Tannins have antibacterial activity, in general, the mechanism is to damage the bacterial cell membrane and induce the formation of complex compound bonds to enzymes or microbial substrates. This study aims to analyze the bioactive compounds contained in C. arthropurpureus in qualitative and quantitative which have an antimicrobial function using high-performance liquid chromatography (HPLC) in the reverse phase C-18 column and screening of antibacterial activity was carried out by disc diffusion method. The results of both qualitative and quantitative analysis by HPLC has obtained the presence of tannin bioactive compounds (1.48 ppm at a retention time of 2.806 minutes) and alkaloids (1.11 ppm at a retention time of 7.015). Moreover, we verified the diameter of inhibition of growth zone against S. aureus at a concentration of 15% extract was 12.80 mm. It was found that the highest percentage of the bioactive compound in C. arthropurpureus is tannin, and that is might an antibacterial agent.

scholarly journals Characterization of Bioactive Compounds in Colored Potato (Solanum Tuberosum L.) Cultivars Grown with Conventional, Organic, and Biodynamic Methods

2020 ◽  
Vol 12 (7) ◽  
pp. 2701 ◽  
Author(s):  
Nijolė Vaitkevičienė ◽  
Jurgita Kulaitienė ◽  
Elvyra Jarienė ◽  
Dovilė Levickienė ◽  
Honorata Danillčenko ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
High Performance ◽  
Solanum Tuberosum L ◽  
Principal Component ◽  
Coumaric Acid ◽  
Chromatography Method ◽  
Organic Cultivation ◽  
Liquid Chromatography Method

The purpose of this research is to evaluate the effect of conventional, biodynamic, and organic cultivation methods on the contents of polyphenols and carotenoids in tubers of potato cultivars with different colored flesh: “Red Emmalie”, “Salad Blue”, “Violetta”, “Tornado”, and “Laura”. These bioactive compounds were determined by the high-performance liquid chromatography method. The data received were analyzed with ANOVA and further explored and visualized by principal component analysis. Higher contents of polyphenols (sum), phenolic acids (sum), chlorogenic acid, p-coumaric acid, and caffeic acid were found in biodynamic and organic samples compared to the conventional tubers. Moreover, organically and biodynamically produced potatoes (except “Salad Blue” cultivar) were significantly richer in flavonoids and anthocyanins. The highest contents of carotenoids (sum), lutein, and β-carotene were found in biodynamic potatoes. Among the tested cultivars, “Tornado”, with white flesh, was richest in polyphenols (sum), phenolic acids (sum), chlorogenic acid, and p-coumaric acid. “Violetta”, with dark purple flesh, accumulated the highest contents of flavonoids (sum), anthocyanins (sum), petunidin-3,5-di-O-glucoside, pelargonidin-3,5-di-O-glucoside, and peonidin-3,5-di-O-glucoside. Carotenoids were only found in “Laura” tubers, and the dominating carotenoid was lutein.

scholarly journals LC-MS and Spectrophotometric Approaches for Evaluation of Bioactive Compounds from Peru Cocoa By-Products for Commercial Applications

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3177
Author(s):  
María de la Luz Cádiz-Gurrea ◽  
Álvaro Fernández-Ochoa ◽  
Francisco Javier Leyva-Jiménez ◽  
Noelia Guerrero-Muñoz ◽  
María del Carmen Villegas-Aguilar ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Negative Impact ◽  
Bioactive Compound ◽  
Added Value ◽  
Industrial Processing ◽  
Pharmaceutical Industries ◽  
High Utility ◽  
Commercial Applications ◽  
By Products

Peru is one of the main areas where there are large cocoa crops with special relevance to the economy of this country. In fact, cocoa is a major, economically important, international crop which has been linked to several benefits, such as anti-allergenic, anti-atherogenic, anti-inflammatory, anti-microbial, anti-oxidant, anti-thrombotic, cardioprotective and vasodilatory properties, relating to its bioactive compound content. However, in cocoa industrial processing, several residues or wastes, which are commonly discarded generating a negative impact on the environment, are produced in large amounts. Some of the cocoa by-products, which go underutilized, could be a good source of bioactive compounds with high utility for the development of innovative products in nutraceutical, medical or pharmaceutical industries. For this reason, the aim of this study is to qualitatively determine the phytochemical composition of husk and bean extracts from different cocoa-growing areas and processes from Peru by high performance liquid chromatography coupled to mass spectrometry. Furthermore, we aim to evaluate their phenolic and flavan-3-ol contents and antioxidant capacities for the purpose of highlighting the potential of cocoa by-products from these cultivars as functional ingredients. In total, 49 chemical compounds were detected in the analyzed extracts. Comparing both husks and beans, bean extracts were characterized by high content in flavonoids whereas husk extracts had a higher content of phenolic acids. The presence of these compounds together with the bioactivity results suggest that these matrices may be further studied for their revaluation in the development of high added-value products in nutraceutical, medical, and pharmaceutical industries.

scholarly journals Microencapsulation of Horseradish (Armoracia rusticana L.) Juice Using Spray-Drying

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1332
Author(s):  
Lolita Tomsone ◽  
Ruta Galoburda ◽  
Zanda Kruma ◽  
Vanessa Durrieu ◽  
Ingmars Cinkmanis
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
High Performance ◽  
Soy Protein Isolate ◽  
Gum Arabic ◽  
Bioactive Compound ◽  
Protein Isolate ◽  
Wall Material ◽  
Total Phenolic ◽  
Wall Materials

Horseradish contains many bioactive compounds with antioxidant activity. The current study aimed to evaluate the effect of various wall materials and their ratios on the physical properties and bioactive-compound retention and stability in microencapsulated horseradish leaf and root juices. Horseradish juice was microencapsulated using maltodextrin, maltodextrin/gum Arabic, soy protein isolate, and starch with three different core-to-wall ratios. The total phenolic, total flavonoid, total flavan-3-ol, and total phenolic-acid contents, as well as antioxidant activity, were determined using spectrophotometric methods, whereas individual phenol profiles were determined by high-performance liquid chromatography (HPLC). Multivariate analysis of variance showed that plant material, wall material, and core-to-wall ratio had a significant effect on the bioactive-compound retention and antioxidant-activity preservation. Microcapsules produced from horseradish leaf juice had a significantly higher content of phenolic compounds and antioxidant activity compared to root-juice microcapsules. However, better retention was observed for microencapsulated horseradish root juice. Maltodextrin and maltodextrin/gum Arabic were the most effective wall materials for the retention of bioactive compounds, while they also had a smaller particle size and better solubility. The horseradish-juice microcapsules possess a high content of rutin. The highest stability of bioactive compounds after storage was found at a core-to-wall ratio of 20:80.

Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

2020 ◽  
Vol 16 ◽  
Author(s):  
Natasa P. Kalogiouri ◽  
Natalia Manousi ◽  
Erwin Rosenberg ◽  
George A. Zachariadis ◽  
Victoria F. Samanidou
Keyword(s):  
Fatty Acids ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
Nutritional Deficiencies ◽  
Analytical Technique ◽  
High Volatility ◽  
Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

scholarly journals Validating Anti-Infective Activity of Pleurotus Opuntiae via Standardization of Its Bioactive Mycoconstituents through Multimodal Biochemical Approach

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 484
Author(s):  
Aprajita Tiwari Pandey ◽  
Ishan Pandey ◽  
Anurag Kanase ◽  
Amita Verma ◽  
Beatriz Garcia-Canibano ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Colorimetric Assay ◽  
Diffusion Method ◽  
Therapeutic Effects ◽  
Medication Regimen ◽  
Methanol Extracts ◽  
Retention Factors ◽  
Before And After ◽  
Solvent Systems

Mushrooms produce a variety of bioactive compounds that are known to have anti-pathogenic properties with safer and effective therapeutic effects in human disease prognosis. The antibacterial activity of ethanol and methanol extracts of Pleurotus opuntiae were checked against pathogenic microorganisms viz. Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis NCIM 2300, Proteus vulgaris NCIM 5266, Serratia marcescens NCIM 2078, Shigella flexeneri NCIM 5265, Moraxella sp. NCIM 2795, Staphylococcus aureus ATCC 25923 by agar well diffusion method at different concentrations of the extracts. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts was determined by INT (Iodonitrotetrazolium chloride) colorimetric assay. Extracts were standardized by thin layer chromatography (TLC) in different solvent systems. The Retention factors (Rf) of different compounds were calculated by high performance TLC (HPTLC) fingerprinting at UV 254, 366, and 540 nm before and after derivatization. The ethanol and methanol extracts of P. opuntiae showed bactericidal activity against all the test pathogens at MIC values of 15.6 to 52.08 mg/mL and 20.81 to 52.08 mg/mL respectively. Whereas the MBC values for ethanol and methanol extract of P. opuntiae against all pathogens were recorded as 26.03 to 62.5 mg/mL and 125 mg/mL respectively. Preliminary mycochemical screening of both the extracts revealed high contents of bioactive compounds. Amongst all the solvent systems used in TLC, the best result was given by chloroform + hexane (8:2) which eluted out 5 different compounds (spots). HPTLC results revealed spots with different Rf values for all the 24 compounds present. Thus, it can be inferred from the present investigation that the mycoconstituents could be an alternative medication regimen and could play a role in new drug discoveries against different infections. Further, the antimicrobial components of these mushrooms can be transformed to bioengineered antimicrobial coatings for surfaces, drug and other hybrid systems for public health implications in combating persistent infections.

scholarly journals Olive Fruit and Leaf Wastes as Bioactive Ingredients for Cosmetics—A Preliminary Study

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 245
Author(s):  
María de la Luz Cádiz-Gurrea ◽  
Diana Pinto ◽  
Cristina Delerue-Matos ◽  
Francisca Rodrigues
Keyword(s):  
Olive Oil ◽  
High Performance ◽  
Value Added ◽  
Bioactive Compound ◽  
In Vitro Assays ◽  
Oil Consumption ◽  
Cosmetic Ingredients ◽  
Bioactive Ingredients ◽  
Olive Industry

Olea europaea cultivar, native in the Mediterranean basin, has expanded worldwide, mainly due to the olive oil industry. This expansion is attributed to the benefits of olive oil consumption, since this product is rich in nutritional and bioactive compounds. However, the olive industry generates high amounts of wastes, which could be related to polluting effects on soil and water. To minimize the environmental impact, different strategies of revalorization have been proposed. In this sense, the aim of this work was to develop high cosmetic value added oleuropein-enriched extracts (O20 and O30), a bioactive compound from olive byproducts, performing a comprehensive characterization using high performance liquid chromatography coupled to mass spectrometry and evaluate their bioactivity by in vitro assays. A total of 49 compounds were detected, with oleuropein and its derivatives widely found in O30 extract, whereas iridoids were mainly detected in O20 extract. Moreover, 10 compounds were detected for the first time in olive leaves. Both extracts demonstrated strong antioxidant and antiradical activities, although O30 showed higher values. In addition, radical oxygen and nitrogen species scavenging and enzyme inhibition values were higher in O30, with the exception of HOCl and hyaluronidase inhibition assays. Regarding cell viability, olive byproduct extracts did not lead to a decrease in keratinocytes viability until 100 µg/mL. All data reported by the present study reflect the potential of industrial byproducts as cosmetic ingredients.

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