scholarly journals Phytochemical Screening and Quantitative Analysis of Coleus arthropurpureus Ethyl Acetate Fraction and Antibacterial Activity Against Staphylococcus aureus

2020 ◽  
Vol 4 (1) ◽  
pp. 17-23
Author(s):  
Indra Lasmana Tarigan ◽  
Ayu Kumala Sari ◽  
Choirul Huda ◽  
Czellicya Jovanncha ◽  
Afidatul Muadifah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Quantitative Analysis ◽  
Bioactive Compounds ◽  
Retention Time ◽  
High Performance ◽  
Bioactive Compound ◽  
Cell System ◽  
Diffusion Method ◽  
Qualitative And Quantitative

Staphylococcus aureus (S. aureus) is a pathogenic microbe that is caused by various diseases in humans and animals. Infectious diseases caused by S. aureus in Asia reached 70% in 2007, while in Indonesia reached 23.5%. The plant provided several bioactive compounds that might function as an antibacterial which inhibits both bacterial growth and damaging the cell system and protein synthesis. Coleus arthropurpureus known contains alkaloids and tannins that supposed to be an antibacterial compound. Tannins have antibacterial activity, in general, the mechanism is to damage the bacterial cell membrane and induce the formation of complex compound bonds to enzymes or microbial substrates. This study aims to analyze the bioactive compounds contained in C. arthropurpureus in qualitative and quantitative which have an antimicrobial function using high-performance liquid chromatography (HPLC) in the reverse phase C-18 column and screening of antibacterial activity was carried out by disc diffusion method. The results of both qualitative and quantitative analysis by HPLC has obtained the presence of tannin bioactive compounds (1.48 ppm at a retention time of 2.806 minutes) and alkaloids (1.11 ppm at a retention time of 7.015). Moreover, we verified the diameter of inhibition of growth zone against S. aureus at a concentration of 15% extract was 12.80 mm. It was found that the highest percentage of the bioactive compound in C. arthropurpureus is tannin, and that is might an antibacterial agent.

scholarly journals The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 56-63
Author(s):  
Julia Nanda Puspita ◽  
Rikhsan Kurniatuhadi ◽  
Rahmawati Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

scholarly journals ISOLASI DAN UJI ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN SPONS Phyllospongia lamellose SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 252
Author(s):  
Sela S Lempoy ◽  
Widya A Lolo ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Isolation And Purification ◽  
Esherichia Coli ◽  
Identification Stage

ABSTRACT Sponges are one source of producing bioactive components from the sea. Bacteria Symbiosis with sponges are thought to have the potential to produce bioactive compounds that have been isolated from sponges. One of the potential of bioactive compounds which have been found and developed from sponges was antibacterial. The aim of this study was to determine the antibacterial activity of bacteria associated with Phyllospongia lamellose sponges against pathogenic bacteria namely Staphylococcus aureus and Esherichia coli and then proceed with identification of biochemistry in isolates which showed the greatest inhibitory activity. Three isolates of sponges symbiont bacteria were obtained through the isolation and purification stage which were then followed by testing of antibacterial activity with paper disk diffusion method. The antibacterial test results showed the diameter of the inhibitory zone against Staphylococcus aureus, were: SL1(8.67 mm), SL2 (9.33 mm) and SL3(9.00 mm) categorized as medium. Whereas the inhibiotion zone shown on Esherichia coli bacteria is also classified as medium, were: SL1(9.67 mm), SL2 (9.00 mm) and SL3 (9.33 mm). The three isolates continued to the identification stage biochemically. Each isolation was assumed as follows: Desulfotomaculum (SL1), Brochothrix (SL2) and Sulfidobacillus (SL3). Keyword         :Phyllospongia lamellose, isolation, biochemically identification ABSTRAK Spons merupakan salah satu sumber penghasil komponen bioaktif yang berasal dari laut. Bakteri yang bersimbiosis dengan spons diduga memiliki potensi dalam menghasilkan senyawa-senyawa bioaktif yang telah diisolasi dari spons. Potensi senyawa bioaktif yang telah ditemukan dan dikembangkan dari spons salah satunya ialah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari bakteri yang berasosiasi dengan spons Phyllospongia lamellose terhadap bakteri patogen yakni Staphylococcus aureus dan Escherichia coli dan kemudian dilanjutkan dengan identifikasi secara biokimia pada isolat yang menunjukkan daya hambat terbesar. Diperoleh 3 isolat bakteri simbion spons melalui tahap isolasi dan purifikasi yang kemudian dilanjutkan dengan pengujian aktivitas antibakteri melalui metode difusi kertas cakram. Hasil uji antibakteri menunjukkan diameter zona hambat terhadap bakteri Staphylococcus aureus yaitu: SL1(8.67 mm), SL2(9.33 mm) dan SL3 (9.00 mm) termasuk dalam kategori sedang. Sedangkan zona hambat yang ditunjukkan terhadap bakteri Escherichia coli juga tergolong kategori sedang yaitu: SL1(9.67 mm), SL2(9.00 mm) dan SL3 (9.33 mm). Ketiga isolat dilanjutkan ke tahap identifikasi secara biokimia. Masing-masing isolat diduga sebagai berikut: Desulfotomaculum (SL1), Brochothrix (SL2) dan Sulfidobacillus (SL3). Kata kunci          :Phyllospongia lamellose, isolasi, identifikasi biokimia.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals Validating Anti-Infective Activity of Pleurotus Opuntiae via Standardization of Its Bioactive Mycoconstituents through Multimodal Biochemical Approach

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 484
Author(s):  
Aprajita Tiwari Pandey ◽  
Ishan Pandey ◽  
Anurag Kanase ◽  
Amita Verma ◽  
Beatriz Garcia-Canibano ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Colorimetric Assay ◽  
Diffusion Method ◽  
Therapeutic Effects ◽  
Medication Regimen ◽  
Methanol Extracts ◽  
Retention Factors ◽  
Before And After ◽  
Solvent Systems

Mushrooms produce a variety of bioactive compounds that are known to have anti-pathogenic properties with safer and effective therapeutic effects in human disease prognosis. The antibacterial activity of ethanol and methanol extracts of Pleurotus opuntiae were checked against pathogenic microorganisms viz. Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis NCIM 2300, Proteus vulgaris NCIM 5266, Serratia marcescens NCIM 2078, Shigella flexeneri NCIM 5265, Moraxella sp. NCIM 2795, Staphylococcus aureus ATCC 25923 by agar well diffusion method at different concentrations of the extracts. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts was determined by INT (Iodonitrotetrazolium chloride) colorimetric assay. Extracts were standardized by thin layer chromatography (TLC) in different solvent systems. The Retention factors (Rf) of different compounds were calculated by high performance TLC (HPTLC) fingerprinting at UV 254, 366, and 540 nm before and after derivatization. The ethanol and methanol extracts of P. opuntiae showed bactericidal activity against all the test pathogens at MIC values of 15.6 to 52.08 mg/mL and 20.81 to 52.08 mg/mL respectively. Whereas the MBC values for ethanol and methanol extract of P. opuntiae against all pathogens were recorded as 26.03 to 62.5 mg/mL and 125 mg/mL respectively. Preliminary mycochemical screening of both the extracts revealed high contents of bioactive compounds. Amongst all the solvent systems used in TLC, the best result was given by chloroform + hexane (8:2) which eluted out 5 different compounds (spots). HPTLC results revealed spots with different Rf values for all the 24 compounds present. Thus, it can be inferred from the present investigation that the mycoconstituents could be an alternative medication regimen and could play a role in new drug discoveries against different infections. Further, the antimicrobial components of these mushrooms can be transformed to bioengineered antimicrobial coatings for surfaces, drug and other hybrid systems for public health implications in combating persistent infections.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals In VitroAntibacterial Potential of Extracts ofSterculia africana,Acacia sieberiana, andCassia abbreviatassp.abbreviataUsed by Yellow Baboons(Papio cynocephalus)for Possible Self-Medication in Mikumi National Park, Tanzania

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Irene Kirabo ◽  
Faith P. Mabiki ◽  
Robinson H. Mdegela ◽  
Christopher J. D. Obbo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Plant Extracts ◽  
National Park ◽  
Nonhuman Primates ◽  
Diffusion Method ◽  
Root Bark ◽  
Self Medication ◽  
Gram Positive ◽  
Acacia Sieberiana

In the animals in general and nonhuman primates in particular self-medication has been widely reported; however, little is still known about the pharmacological activity of the extracts present in their daily diet. Thein vitroantibacterial activity of the stem, root bark, and leaf extracts of three selected plants on which yellow baboons feed in an unusual manner in Mikumi National Park, Tanzania, was evaluated. Crude plant extracts were tested against Gram positive and Gram negative bacteria of medical and veterinary importance employing a modified agar well diffusion method and Minimum Inhibitory Concentration (MIC) technique. The lowest MIC value for Gram positive strains was 0.31 mg/ml demonstrated byCassia abbreviatassp.abbreviataagainstStaphylococcus aureus(ATCC 25923). The highest susceptibility to the ethanol plant extracts was exhibited byPseudomonas aeruginosa,Escherichia coli,andStaphylococcus aureus,examples of microbes that affect both human and nonhuman primates. These findings demonstrate that the plant extracts fromSterculia africana,Acacia sieberiana,andCassia abbreviatassp.abbreviatahave antibacterial activity and may be used as feed for their prophylactic benefits. Remarkably, the lowest MIC of 0.16 mg/ml was only 16-fold weaker than Gentamicin, a standard drug.

scholarly journals Optical characterization and concentration-dependent antibacterial activity of silver nanoparticles (AgNPs) using aqueous Melastoma malabathricum extract against Staphylococcus aureus

2020 ◽  
Vol 11 (4) ◽  
pp. 5382-5387
Author(s):  
Irshad Ul Haq Bhat ◽  
Maisarah Binti Alias
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Functional Groups ◽  
Diffusion Method ◽  
Synthetic Approach ◽  
Potential Candidate ◽  
X Ray ◽  
Melastoma Malabathricum ◽  
Resonance Band

The approach towards green synthetic methods has been enormously encouraged to synthesise nanoparticles for various uses. In this study, the one-pot synthetic method was adapted to synthesise silver nanoparticles (AgNPs) using Melastoma malabathricum (M. malabathricum) aqueous extract. The formation of AgNPs was confirmed by observing the results obtained by optical characterisation methods. The plasma resonance band along with shoulder at 375 nm and 595 nm, respectively, in Uv-Visible spectra supported the conversion of silver (Ag) to AgNPs reduced by functional groups present in the plant extract. The size of AgNPs was 31 nm and cubic in shape as confirmed by X-ray diffractometry (XRD) using Scherer equation. X-Ray Fluorescence (XRF) results also confirmed the presence of silver. The FTIR characterisation confirmed the presence of reducing functional groups. The antibacterial activity of AgNPs against Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration of AgNPs, and enhanced inhibition zone was observed. The AgNPs obtained can be further explored against different bacterial strains and can a potential candidate as an antibacterial agent using the green synthetic approach.

scholarly journals Aktivitas Antibakteri dan Antioksidan Asam Galat dari Kulit Buah Lokal yang Diproduksi dengan Tanase

2018 ◽  
Vol 14 (1) ◽  
pp. 148
Author(s):  
Eka Junaidi ◽  
Yunita Arian Sani Anwar
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Gallic Acid ◽  
Diffusion Method ◽  
Syzygium Cumini ◽  
Garcinia Mangostana ◽  
Dpph Method ◽  
Fruit Waste

<p>Penelitian ini merupakan lanjutan dari penelitian sebelumnya tentang produksi asam galat dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (<em>Baccaurea racemosa</em> Muell.Arg), kulit buah juwet (<em>Syzygium cumini</em>), dan kulit buah manggis (<em>Garcinia mangostana</em>). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri <em>Escherichia coli</em> dan <em>Staphylococcus aureus</em>. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC<sub>50</sub> masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri <em>Escherichia coli</em> dan <em>Staphylococcus aureus </em>yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.</p><p><strong>Antibacterial and antioxidant activity of gallic acid from peel local fruit which produced by tannase. </strong>This research is a continuation of the previous research about the gallic acid production from peel fruit local in Lombok. The local fruit waste<em> </em>used<em> </em>are<em> kepundung rind (Baccaurea racemosa</em> Muell.Arg)<em>, juwet rind </em>(<em>Syzygium cumini</em>) <em>and mangosteen rind </em>(<em>Garcinia mangostana</em>)<em>. </em>In this research, the produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method to bacteria <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. The results show that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC<sub>50</sub> values of 5,95; 5,96; and 5,92,  respectively. Gallic acid not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result show that gallic acid from local fruit waste did not provide an antibacterial activity.</p>

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

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