scholarly journals Enterococcus faecalis causes osteitis deformans in a Golden Lancehead snake (Bothrops insularis): a case report

2020 ◽  
Vol 57 (4) ◽  
pp. e163926
Author(s):  
Viviane Campos Garcia ◽  
Pedro Enrique Navas-Suárez ◽  
Ana Carolina Brandão de Campos Fonseca-Pinto ◽  
Silvana Maria Unruh ◽  
Terezinha Knöbl ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Blood Cells ◽  
Bacterial Culture ◽  
New Bone Formation ◽  
Osteitis Deformans ◽  
Locomotor Disability ◽  
Ultrasound Computed Tomography ◽  
Bone Changes ◽  
First Time ◽  
Bone Proliferation

Osteitis deformans (Paget’s disease) is a chronic bone disorder characterized by excessive osteoclast-mediated bone resorption followed by new bone formation. The present paper reports this condition in an 18-year-old captive golden lancehead (Bothrops insularis) from Brazil. This patient initially exhibited anorexia and swelling in the middle third of the spine associated with locomotor disability. For diagnosis, radiography, ultrasound, computed tomography, cytology, and microbiological culture were performed. Diagnostic imaging showed bone changes, vertebral fusion, and bone proliferation. Cytology revealed blood cells how toxic heterophiles, reactive monocytes, young red blood cells, and polychromasia compatible with an infectious process. A bacterial culture identified an ampicillin-susceptible strain of Enterococcus faecalis. Antibiotic treatment was promptly started, but the snake died 25 days later. Histopathologically, the bone tissue showed a generalized thickening of the vertebral trabeculae. For the first time, the presence of E. faecalis associated with the development of osteitis deformans in snakes was presented.

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

scholarly journals Effect of Antibacterial Root Canal Sealer on Persistent Apical Periodontitis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 741
Author(s):  
Zheng Wang ◽  
Ge Yang ◽  
Biao Ren ◽  
Yuan Gao ◽  
Xian Peng ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Therapeutic Effect ◽  
Root Canal ◽  
Apical Periodontitis ◽  
Control Group ◽  
Root Canal Sealer ◽  
Root Canal Filling ◽  
First Time ◽  
Cone Beam Computer Tomography ◽  
Better Than

The infection of Enterococcus faecalis and its interacting microorganisms in the root canal could cause persistent apical periodontitis (AP). Antibacterial root canal sealer has favorable prospects to inhibit biofilms. The purpose of this study was to investigated the antibacterial effect of root canal sealer containing dimethylaminododecyl methacrylate (DMADDM) on persistent AP in beagle dogs for the first time. Persistent AP was established by a two-step infection with Enterococcus faecalis and multi-bacteria (Enterococcus faecalis, Lactobacillus acidophilus, Actinomycesnaeslundii, Streptococcus gordonii). Root canal sealer containing DMADDM (0%, 1.25%, 2.5%) was used to complete root canal filling. The volume of lesions and inflammatory grade in the apical area were evaluated by cone beam computer tomography (CBCT) and hematoxylin-eosin staining. Both Enterococcus-faecalis- and multi-bacteria-induced persistent AP caused severe apical destruction, and there were no significant differences in pathogenicity between them. DMADDM-modified sealer significantly reduced the volume of periapical lesion and inflammatory grade compared with the control group, among them, the therapeutic effect of the 2.5% group was better than the 1.25% group. In addition, E.faecalis-induced reinfection was more sensitive to the 2.5% group than multi-bacteria reinfection. This study shows that root canal sealer containing DMADDM had a remarkable therapeutic effect on persistent AP, especially on E. faecalis-induced reinfection.

scholarly journals Effect of Pegbovigrastim on Hematological Profile of Simmental Dairy Cows during the Transition Period

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 841 ◽  
Author(s):  
Francesca Trimboli ◽  
Valeria Maria Morittu ◽  
Antonio Di Loria ◽  
Andrea Minuti ◽  
Antonella Anna Spina ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Blood Cells ◽  
Transition Period ◽  
Lower Percentage ◽  
Control Group ◽  
Volume Distribution ◽  
Polymorphonuclear Cells ◽  
Distribution Width ◽  
Hematological Profile ◽  
First Time

Pegbovigrastim is a long-acting analog of recombinant bovine granulocyte colony-stimulating factor, that promotes and increases the count and functionality of polymorphonuclear cells in dairy cows. The present study aimed to explore, for the first time in Simmental cows, the clinical and hematological effect of pegbovigrastim during the transition period (TP). Cows were randomly assigned into two groups: treated group (PEG; n = 16) received pegbovigrastim at approximately 7 days before expected parturition and within 6 h after calving, and control group (CTR; n = 16) received saline solution. Blood samples were obtained at −7, 0, 1, 3, 7, 14, 21, and 30 days relative to calving. PEG group showed white blood cells (WBC) count consistently higher compared with CTR group (p < 0.001) until to 3 weeks after calving. Neutrophils remained higher in PEG group (p < 0.001) up to three weeks after calving, compared with CTR group, with slight increment of band cells. Moreover, PEG group displayed a lower index of myeloperoxidase at 1, 3, and 7 days after calving (p < 0.01) compared with CTR. Basophils and lymphocytes showed a similar trend to those observed for neutrophils at 1 day after calving in PEG group. Finally, monocytes remained markedly elevated until 3 days after calving in PEG compared to CTR group (p < 0.001), whereas in PEG group, eosinophils population showed lower percentage values at 1 and 3 days after calving but higher values at 30 days compared with CTR group. PEG group was characterized by lower red blood cells (RBCs) count compared with CTR group (p < 0.05) and higher % of red cell volume distribution width (RDW) from week 2 and mean corpuscular volume (MCV) at 30 days after calving. In addition, the mean platelet volume (MPV) was significantly higher in PEG group at calving, 1, 3, and 7 days after calving compared with CTR group (p < 0.05). For the first time, we described the effect of pegbovigrastim in a breed not specialized exclusively in milk production as Holstein, but with dual purpose (meat and milk), evaluating the complete hematological profile in cows during the transition period. These results provide evidence on the proliferative effect of pegbovigrastim on WBC in Simmental breed highlighting its possible side effect on RBCs.

scholarly journals PERPHERAL BLOOD CELL MITOCHONDRIAL DYSFUNCTION IN MYELODYSPLASTIC SYNDROMECAN BE IMPROVED BY A COMBINATION OF COENZYME Q10 AND CARNITINE

2020 ◽  
Vol 12 (1) ◽  
pp. e2020072
Author(s):  
Kalman Filanovsky ◽  
Michal Haran ◽  
Vita Mirkin ◽  
Andrei Braester ◽  
Olga Shevetz ◽  
...  
Keyword(s):  
Myelodysplastic Syndrome ◽  
Coenzyme Q10 ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Food Supplements ◽  
Peripheral Blood Cells ◽  
Significant Impairment ◽  
Effect Of Food ◽  
Metabolic Properties ◽  
First Time

Structural mitochondrial abnormalities as well as genetic aberrations in mitochondrial proteins have been known in Myelodysplastic syndrome (MDS) , yet there is currently little data regarding the metabolic properties and energy production of MDS cells. In the current study we used state-of-the-art methods to assess OXPHOS in peripheral blood cells obtained from MDS patients and healthy controls We then assessed the effect of food supplements- Coenzyme Q10 and carnitine on mitochondrial function and hematological response .We show here for the first time that in low risk MDS there is a significant impairment of mitochondrial respiration in peripheral blood cells and this can be improved with food supplements. We also show that such myelodysplastic syndrome, mitochondria, oxidative phosphorylation, coenzyme Q10, seahorse XF analyzer. supplements lead to improvement in cytopenia's and quality of life.

scholarly journals Livestock-associated meticillin-resistant Staphylococcus aureus in a young harbour seal (Phoca vitulina) with endocarditis

2019 ◽  
Vol 7 (3) ◽  
pp. e000886 ◽  
Author(s):  
Ana Rubio-Garcia ◽  
John W A Rossen ◽  
Jaap A Wagenaar ◽  
Alex W Friedrich ◽  
Jan H van Zeijl
Keyword(s):  
Staphylococcus Aureus ◽  
Marine Mammals ◽  
Aortic Wall ◽  
Bacterial Culture ◽  
Histopathological Examination ◽  
Phoca Vitulina ◽  
Antimicrobial Treatment ◽  
Harbour Seal ◽  
First Time ◽  
Secondary Bacterial Pneumonia

A five-month-old male harbour seal was admitted for rehabilitation to the Sealcentre Pieterburen on November 16, 2015. During initial veterinary examination parasitic pneumonia and secondary bacterial pneumonia were suspected. Therefore, the seal received antiparasitic and antimicrobial treatment and appeared to recover but died unexpectedly after several weeks. Postmortem examination revealed a perforation in the aortic wall and histopathological examination of the aorta revealed mural necrosis with haemorrhage and suppurative to mixed inflammation. Bacterial culture resulted in isolation of a meticillin-resistant Staphylococcus aureus (MRSA) from the pericardial effusion. Subsequent culture of rectal swabs collected at arrival and during rehabilitation showed that the animal was already colonised with MRSA when admitted to the Sealcentre. MRSA has been isolated from marine mammals before, however, to our knowledge this is the first report of MRSA-associated endocarditis in seals and the first time that livestock-associated MRSA is reported in seals.

EFFECT OF STENOSIS SEVERITY ON SHEAR-INDUCED DIFFUSION OF RED BLOOD CELLS IN CORONARY ARTERIES

2019 ◽  
Vol 19 (05) ◽  
pp. 1950034 ◽  
Author(s):  
ABDULRAJAK BURADI ◽  
SUMANT MORAB ◽  
ARUN MAHALINGAM
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mixture Theory ◽  
Theory Approach ◽  
Newtonian Viscosity ◽  
Maximum Fluctuation ◽  
Stenosis Severity ◽  
Flux Model ◽  
First Time ◽  
Arterial Endothelial Cells

In large blood vessels, migration of red blood cells (RBCs) affects the concentration of platelets and the transport of oxygen to the arterial endothelial cells. In this work, we investigate the locations where hydrodynamic diffusion of RBCs occurs and the effects of stenosis severity on shear-induced diffusion (SID) of RBCs, concentration distribution and wall shear stress (WSS). For the first time, multiphase mixture theory approach with Phillips shear-induced diffusive flux model coupled with Quemada non-Newtonian viscosity model has been applied to numerically simulate the RBCs macroscopic behavior in four different degrees of stenosis (DOS) geometries, viz., 30%, 50%, 70% and 85%. Considering SID of RBCs, the calculated average WSS increased by 77.70% which emphasises the importance of SID in predicting hemodynamic parameters. At the stenosis throat, it was observed that 85% DOS model had the lowest concentration of RBCs near the wall and highest concentration at the center. For the stenosis models with 70% and 85% DOS, the RBC lumen wall concentration at the distal section of stenosis becomes inhomogeneous with the maximum fluctuation of 1.568%. Finally, the wall regions with low WSS and low RBC concentrations correlate well with the atherosclerosis sites observed clinically.

scholarly journals Monitoring the side effects with DSC caused by cyclophosphamide treatment

2019 ◽  
Vol 142 (2) ◽  
pp. 765-770
Author(s):  
Péter Farkas ◽  
Franciska Könczöl ◽  
Dénes Lőrinczy
Keyword(s):  
Side Effects ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Peripheral Nerves ◽  
Chemotherapeutic Agents ◽  
Cyclophosphamide Treatment ◽  
Micro Dsc ◽  
Induced Changes ◽  
First Time ◽  
Dose Dependent

AbstractPolyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of a cancer therapy (using cyclophosphamide) as well. In the daily use, it is very important to know the kinetics and metabolism of anticancer drugs because this way we can estimate their undesirable consequences to avoid the unwanted complications. Nowadays, the application of DSC in diagnosis of different diseases using blood compounds is increasing; therefore, the purpose of recent study was to introduce at the first time the DSC in the detection of cyclophosphamide-induced changes in plasma and red blood cells in case of an experimental animal model. Guinea pigs (Cavia porcellus, n = 60, in 12 different groups) underwent to cyclophosphamide treatment administrated intraperitoneally with the dose comparable to the human dosage. At the end of treatments, the animals were euthanized, and the experimental samples (plasma end red blood cells) were analyzed by a SETARAM Micro DSC-II calorimeter. The denaturation temperatures and the calorimetric enthalpies were calculated from the heat flow curves. Our results exhibited a dose-dependent difference between thermal parameters of untreated and treated samples, demonstrating that DSC is applicable in this field too. After deconvolution of DSC scans, the changes could be assigned to the attacked compounds. Recently published papers call the attention for this technique more frequently in the medical diagnosis because this way from small amount of sample very quickly and economically possible to detect and predict the expected and unwanted side effects of chemotherapeutic agents.

scholarly journals First Report of the Multidrug Resistance Genecfrin Enterococcus faecalis of Animal Origin

2011 ◽  
Vol 56 (3) ◽  
pp. 1650-1654 ◽  
Author(s):  
Yang Liu ◽  
Yang Wang ◽  
Congming Wu ◽  
Zhangqi Shen ◽  
Stefan Schwarz ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Enterococcus Faecalis ◽  
Insertion Sequence ◽  
Recombination Process ◽  
Inverse Pcr ◽  
Animal Origin ◽  
Content Type ◽  
First Report ◽  
First Time

ABSTRACTThe multiresistance genecfrwas identified for the first time in anEnterococcus faecalisisolate of animal origin. The 32,388-bp plasmid pEF-01, which carried thecfrgene, was sequenced completely. Three copies of the insertion sequence IS1216were identified in pEF-01, and the detection of acfr- and IS1216-containing amplicon by inverse PCR suggests that IS1216may play a role in the dissemination ofcfrby a recombination process.

Donor-derived DNA in fingernails among recipients of allogeneic hematopoietic stem-cell transplants

Blood ◽  
2007 ◽  
Vol 110 (7) ◽  
pp. 2231-2234 ◽  
Author(s):  
Daisuke Imanishi ◽  
Yasushi Miyazaki ◽  
Reishi Yamasaki ◽  
Yasushi Sawayama ◽  
Jun Taguchi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Blood Cells ◽  
Hematopoietic Stem ◽  
Donor Cells ◽  
Cell Transplants ◽  
First Time ◽  
Hematopoietic Stem Cell Transplants ◽  
Short Tandem

To examine whether donor-derived cells could exist in nonhematopoietic tissues of recipients after allogeneic hematopoietic stem-cell transplantation, we examined the patterns of the short tandem repeat (STR) of DNA extracted from fingernail clippings of recipients so that the contamination of blood cells was excluded. All 21 patients reached donor-derived hematopoiesis after transplantation and 20 of them were in remission of the primary diseases at the time of sampling. Compared with the STRs of donor cells, among 9 of 21 patients, DNA extracted from fingernail samples showed coexistence of the donor pattern of the STRs, sharing from 8.9% to 72.9% of total STR areas. Time from transplantation to sampling was from 305 to 2399 days among positive cases. These results demonstrate for the first time the existence of stable contribution of donor cells in fingernails among recipients of allogeneic hematopoietic stem cells.

scholarly journals Evaluation of an Integrated Smart Sensor System for Real-Time Characterization and Digitalization of Postoperative Abdominal Drain Output: A Pilot Study

2021 ◽  
pp. 155335062110314
Author(s):  
Mario V. Roser ◽  
Alexander H. R. Frank ◽  
Lea Henrichs ◽  
Christian Heiliger ◽  
Dorian Andrade ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Real Time ◽  
Blood Cells ◽  
Modern Medicine ◽  
Sensor System ◽  
Smart Sensor ◽  
Routine Laboratory ◽  
Laboratory Measurements ◽  
Abdominal Drain ◽  
First Time

Background: For centuries, surgeons have relied on surgical drains during postoperative care. Despite all advances in modern medicine and the area of digitalization, as of today, most if not all assessment of abdominal secretions excreted via surgical drains are carried out manually. We here introduce a novel integrated Smart Sensor System ( Smart Drain) that allows for real-time characterization and digitalization of postoperative abdominal drain output at the patient’s bedside. Methods: A prototype of the Smart Drain was developed using a sophisticated spectrometer for assessment of drain output. The prototype measures 10 × 6 × 6 cm and therefore easily fits at the bedside. At the time of measurement with our Smart Drain, the drain output was additionally sent off to be analyzed in our routine laboratory for typical markers of interest in abdominal surgery such as bilirubin, lipase, amylase, triglycerides, urea, protein, and red blood cells. A total of 45 samples from 19 patients were included. Results: The measurements generated were found to correlate with conventional laboratory measurements for bilirubin (r = .658, P = .000), lipase (r = .490, P = .002), amylase (r = .571, P = .000), triglycerides (r = .803, P = .000), urea (r = .326, P = .033), protein (r = .387, P = .012), and red blood cells (r = .904, P = .000). Conclusions: To our best knowledge, for the first time we describe a device using a sophisticated spectrometer that allows for real-time characterization and digitalization of postoperative abdominal drain output at the patient’s bedside.

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