Polyelectrolyte Membrane with Hydroxyapatite and Silver Nanoparticles as a Material for Modern Wound Dressings

2020 ◽  
Vol 16 (5) ◽  
pp. 702-714
Author(s):  
Magdalena Antosiak-Iwańska ◽  
Paweł Bącal ◽  
Beata Kazimierczak ◽  
Angelika Kwiatkowska ◽  
Ewa Godlewska ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Healing Process ◽  
Antimicrobial Properties ◽  
Model System ◽  
Wound Dressings ◽  
Eukaryotic Cells ◽  
Membrane Material ◽  
Bacterial Strains ◽  
Polyelectrolyte Membrane ◽  
Membrane Scaffold

Modern wound dressings not only play a covering role but also facilitate the function of the wound, contributing to a faster healing process. In this paper, we present a polyelectrolyte system with nanosized elements that could stimulate the growth of eukaryotic cells while providing antimicrobial properties, which may be recommended as a potential dressing material. The proposed platform consisted of polyethyleneimine, hydroxyapatite, and silver nanoparticles and was characterized using various macroscopic techniques. The constructed membrane scaffold was evaluated with immobilized WEHI 164 cells as a model system for cells sustained at the interface of bone and skin. Moreover, the bacteriostatic function of the designed membrane material was evaluated using different bacterial strains.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

scholarly journals Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Haroon Ashraf ◽  
Kerri Font ◽  
Charles Powell ◽  
Michael Schurr
Keyword(s):  
Bacterial Species ◽  
Healing Process ◽  
Antimicrobial Properties ◽  
Positive Control ◽  
Negative Control ◽  
Collagen Membrane ◽  
Wound Healing Process ◽  
Bacterial Strains ◽  
Oral Microbes ◽  
Negative Controls

Objective. The aim of this study was to evaluate wound biomodification by assessing antimicrobial properties present within a human-derived composite amnion-chorion membrane (ACM). Methods. Membranes analyzed were the human-derived ACM BioXclude™ and the porcine-derived collagen membrane Bio-Gide®. Paper discs with and without tetracycline served as positive and negative controls, respectively. The same number of colony-forming units per milliliter for each bacterial species (Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus oralis) was inoculated on each of the discs. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth. Results. The ACM inhibited growth at all time points, with all bacterial strains, identical to the negative control tetracycline discs. The collagen membrane and positive controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period. P<0.05 for microbial growth on ACM or negative control vs. either collagen membrane or positive control. Conclusion. ACM was proven to be as bactericidal as paper discs inoculated with tetracycline at its minimum bactericidal concentration. The ACM bactericidal property may be beneficial in the early wound healing process.

scholarly journals Chitosan/Glycosaminoglycan Scaffolds: The Role of Silver Nanoparticles to Control Microbial Infections in Wound Healing

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1207 ◽  
Author(s):  
Giuseppina Sandri ◽  
Dalila Miele ◽  
Angela Faccendini ◽  
Maria Cristina Bonferoni ◽  
Silvia Rossi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Chondroitin Sulfate ◽  
Chronic Wounds ◽  
Healing Process ◽  
Antimicrobial Properties ◽  
Fibroblast Proliferation ◽  
Nanofibrous Scaffolds ◽  
Microbial Infections ◽  
Traumatic Wound ◽  
Scaffold Degradation

Cutaneous wounds represent a major issue in medical care, with approximately 300 million chronic and 100 million traumatic wound patients worldwide, and microbial infections slow the healing process. The aim of this work was to develop electrospun scaffolds loaded with silver nanoparticles (AgNPs) to enhance cutaneous healing, preventing wound infections. AgNPs were directly added to polymeric blends based on chitosan (CH) and pullulan (PUL) with hyaluronic acid (HA) or chondroitin sulfate (CS) to be electrospun obtaining nanofibrous scaffolds. Moreover, a scaffold based on CH and PUL and loaded with AgNPs was prepared as a comparison. The scaffolds were characterized by chemico–physical properties, enzymatic degradation, biocompatibility, and antimicrobial properties. All the scaffolds were based on nanofibers (diameters about 500 nm) and the presence of AgNPs was evidenced by TEM and did not modify their morphology. The scaffold degradation was proven by means of lysozyme. Moreover, the AgNPs loaded scaffolds were characterized by a good propensity to promote fibroblast proliferation, avoiding the toxic effect of silver. Furthermore, scaffolds preserved AgNP antimicrobial properties, although silver was entrapped into nanofibers. Chitosan/chondroitin sulfate scaffold loaded with AgNPs demonstrated promotion of fibroblast proliferation and to possess antimicrobial properties, thus representing an interesting tool for the treatment of chronic wounds.

scholarly journals Antibacterial Effect of Colloidal Suspensions Varying in Silver Nanoparticles and Ions Concentrations

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Varvara Platania ◽  
Alexandra Kaldeli-Kerou ◽  
Theodora Karamanidou ◽  
Maria Kouki ◽  
Alexander Tsouknidas ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Colloidal Suspensions ◽  
Silver Ions ◽  
Cellular Level ◽  
Bacterial Strains ◽  
Three Samples ◽  
Flow Filtration

A lot of effort has been dedicated recently to provide a better insight into the mechanism of the antibacterial activity of silver nanoparticles (AgNPs) colloidal suspensions and their released silver ionic counterparts. However, there is no consistency regarding whether the antibacterial effect displayed at cellular level originates from the AgNPs or their ionic constitutes. To address this issue, three colloidal suspensions exhibiting different ratios of AgNPs/silver ions were synthesized by a wet chemistry method in conjunction with tangential flow filtration, and were characterized and evaluated for their antimicrobial properties against two gram-negative, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), and two gram-positive, Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), bacterial strains. The produced samples contained 25% AgNPs and 75% Ag ions (AgNP_25), 50% AgNPs and 50% Ag ions (AgNP_50), and 100% AgNPs (AgNP_100). The sample AgNP_100 demonstrated the lowest minimum inhibitory concentration values ranging from 4.6 to 15.6 ppm for all four bacterial strains, while all three samples indicated minimum bactericidal concentration (MBC) values ranging from 16.6 ppm to 62.5 ppm against all strains. An increase in silver ions content results in higher bactericidal activity. All three samples were found to lead to a significant morphological damage by disruption of the bacterial cell membranes as analyzed by means of scanning electron microscopy (SEM). The growth kinetics demonstrated that all three samples were able to reduce the bacterial population at a concentration of 3.1 ppm. SEM and growth kinetic data underline that S. epidermidis is the most sensitive among all strains against the investigated samples. Our results showed that all three AgNPs colloidal suspensions exhibited strong antibacterial properties and, thus, they can be applied in medical devices and antimicrobial control systems.

scholarly journals Nanostructured coatings based on silver nanoparticles with applications in anti-infectious therapy

2019 ◽  
Vol 1 (1) ◽  
pp. 17-18
Keyword(s):  
Silver Nanoparticles ◽  
Treatment Resistance ◽  
Wound Dressings ◽  
Cellular Viability ◽  
Bacterial Strains ◽  
Treatment Results ◽  
Wound Site ◽  
Polymeric Layer ◽  
Biological Tests ◽  
Healing Of Wounds

The increasingly concerning cases regarding patients presenting wound infections caused by antibiotic treatment resistance for multiple bacterial strains led to advanced researches that help with resolution of this common problem that may ultimately result in death. Regarding healing of wounds caused by trauma, many parameters that influence after treatment results need to be taken into consideration, like choosing the cleansing technique in the first stage of the wound and optimizing the type of wound dressing used, preferable to present all the characteristics necessary for the type of wound and cicatrization of the wound to be hard to identify after healing [1,2]. For this purpose, the theme of this paper targets the use of wound dressings functionalized with silver nanoparticles and polymeric layer of alginate in anti-infectious therapy, which contributes successfully in resolving the problem about the possibility of infection at the wound site and sustain a faster healing. The paper presents the analysis methods applied on the samples, using SEM, TEM, XRD and biological tests of cellular viability and antimicrobial tests, which demonstrated effective results of the silver nanoparticles in anti-infectious therapy.

scholarly journals Effect of AgNPs on the human reconstructed epidermis

2018 ◽  
Vol 11 (4) ◽  
pp. 289-293 ◽  
Author(s):  
Jana Franková ◽  
Jana Juráňová ◽  
Vojtěch Kamarád ◽  
Bohumil Zálešák ◽  
Jitka Ulrichová
Keyword(s):  
Silver Nanoparticles ◽  
Inflammatory Cytokines ◽  
Morphological Changes ◽  
Antimicrobial Properties ◽  
Wound Dressings ◽  
Skin Cells ◽  
Reconstructed Epidermis ◽  
Wide Range ◽  
Negative Effect ◽  
Pro Inflammatory Cytokines

Abstract Nanoparticles are utilized in a wide range of industries. The most studied silver nanoparticles (AgNPs) are used in medicine and also in several wound dressings due to their antimicrobial properties. The inflammatory response or potential morphological changes of skin cells after their application are not well known yet. In our study we used the model of human reconstructed epidermis (RHE), prepared in our laboratory, to evaluate whether the AgNPs penetrate through RHE, induce some morphological changes of keratinocytes or influence the production of pro-inflammatory cytokines (IL-6 and IL-8). After the application of three different concentrations (25 ppm, 2.5 ppm, 0.25 ppm) of AgNPs to of RHE for 24 hours we verified that AgNPs did not affect the production of pro-inflammatory cytokines (IL-6 and IL-8) and neither did they influence the expression of keratin K14 and loricrin. The morphology of the cells was likewise unchanged. Based on these results we conclude that AgNPs do not have any negative effect on the morphological changes and do not increase the production of pro-inflammatory cytokines.

scholarly journals FABRICATION OF NANOFIBRES BY ELECTROSPINNING USING KERATIN FROM WASTE CHICKEN FEATHERS, PVA AND AgNPs

2019 ◽  
pp. 78-84
Author(s):  
VARUN DINESH MADAPALLY ◽  
PANDIMADEVI M.
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Average Diameter ◽  
Electrospinning Process ◽  
Wound Dressings ◽  
Electrospinning Technique ◽  
Chicken Feathers ◽  
Tridax Procumbens ◽  
Film Forming ◽  
Waste Chicken Feathers

Objective: To prepare and characterise keratin from chicken feathers (CF), collected from the slaughter house, and to blend with poly vinly alcohol (PVA) and biosynthesised silver nanoparticles (AgNPs) and to convert into nanofibers by an elctrospinning process. Methods: The extraction of keratin from chicken feathers was done by sodium m-bisulphite. The solution was subjected to ammonium sulphate precipitation to separate keratin. The nanoparticles was synthesised using tridax procumbens. The isolated keratin and PVA was mixed in the ration 0f 50:50 with 1 ml of biosynthesised nanoparticles was blended and made into nanofibres by electrospinning technique. Results: The precipitated protein was analysed using FT-IR analysis confirming the presence of β-keratin in the sample isolated from chicken feathers and the concentration of keratin was estimated to be 1.85 g/ml. PVA solution with 4% w/v had the best film forming ability. The solution containing keratin, PVA and silver nanoparticles was prepared in various proportions. These solutions when subjected to electrospinning, fibrous network was observed in 50:50 (PVA: Keratin) ratio with 1 ml of synthesised silver nanoparticle solution. Hydrogen bonding between keratin and PVA indicated in the XRD analysis showed successful film forming of the nanofiber, the DSC analysis also showed similar results as the obtained peak was at 214 °C which is in between the characteristic heat degradation temperature of both the keratin and PVA. The thermogravimetric analysis (TGA) showed high thermal stability as the complete degradation of the nanofiber was observed at 420 °C. Incorporation of metal nanoparticles by herbal approach using tridax procumbens in the nanofibers provided the antimicrobial properties. The nanofibres obtained by electrospinning process appeared stable and continous for solutions containing no more than 50% wt of CF. The average diameter of the nanofibres increased as the CF content increased. Conclusion: Keratin isolated from the waste chicken feathers impregnated with biosyntheised silver nanoparticles using tridax procumbens and PVA can be converted into nanofibers by electrospinning process. Thus, the biocomposite nano fibers are shown as a novel eco-friendly material that must be adequately applied in the development of green composites for the biomedical applications such as wound dressings.

scholarly journals Chitosan as a Wound Dressing Starting Material: Antimicrobial Properties and Mode of Action

2019 ◽  
Vol 20 (23) ◽  
pp. 5889 ◽  
Author(s):  
Matica ◽  
Aachmann ◽  
Tøndervik ◽  
Sletta ◽  
Ostafe
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Antimicrobial Agents ◽  
Healing Process ◽  
Antimicrobial Properties ◽  
Starting Material ◽  
Wound Dressings ◽  
Current Status ◽  
Natural Polymers ◽  
Promising Source

Fighting bacterial resistance is one of the concerns in modern days, as antibiotics remain the main resource of bacterial control. Data shows that for every antibiotic developed, there is a microorganism that becomes resistant to it. Natural polymers, as the source of antibacterial agents, offer a new way to fight bacterial infection. The advantage over conventional synthetic antibiotics is that natural antimicrobial agents are biocompatible, non-toxic, and inexpensive. Chitosan is one of the natural polymers that represent a very promising source for the development of antimicrobial agents. In addition, chitosan is biodegradable, non-toxic, and most importantly, promotes wound healing, features that makes it suitable as a starting material for wound dressings. This paper reviews the antimicrobial properties of chitosan and describes the mechanisms of action toward microbial cells as well as the interactions with mammalian cells in terms of wound healing process. Finally, the applications of chitosan as a wound-dressing material are discussed along with the current status of chitosan-based wound dressings existing on the market.

scholarly journals Green Synthesis, Characterization and Antimicrobial Evaluation of Silver Nanoparticles for an Intracanal Dressing

2020 ◽  
Vol 31 (5) ◽  
pp. 485-492
Author(s):  
João Felipe Bonatto Bruniera ◽  
Leonado Gabriel-Silva ◽  
Rafael Silva Goulart ◽  
Yara Teresinha Corrêa Silva-Sousa ◽  
Marilisa Guimarães Lara ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Clinical Laboratory ◽  
Antimicrobial Properties ◽  
Absorption Spectrometry ◽  
Minimal Bactericidal Concentration ◽  
Bacterial Strains ◽  
Molecular Absorption Spectrometry ◽  
Antimicrobial Evaluation

Abstract Green chemistry has been applied in different areas due to the growing demands for renewable processes and one of them is nanotechnology. The aim of this study was to characterize a formulation containing silver nanoparticles (AgNPs) produced by a green synthesis and to evaluate its antimicrobial activity. The formulation will be used as an intracanal dressing exploiting the AgNPs’ antimicrobial properties, which are crucial to prevent infections and bacterial reinfections that can compromise endodontic treatments. In the green synthesis, silver nitrate was employed as the precursor salt, maltose as a reducing agent, and gelatin as a stabilizing agent. The formulation was prepared mixing 50 % of a liquid containing the AgNPs and 50 % of hydroxyethylcellulose gel at 1.5 % with proper evaluation of the process inherent parameters. Techniques such as molecular absorption spectrometry and dynamic light scattering were used in characterization step. The antimicrobial activity of the AgNPs against Escherichia coli ATCC 25922, Enterococcus faecalis NCTC 775, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Streptococcus mutans ATCC 25175 was verified according to National Comittee for Clinical Laboratory Standards (NCCLS) by determining minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The obtained results indicated the formulation containing AgNPs produced by a green synthesis was properly characterized by the selected techniques. Furthermore, the formulation assessment proved that it is suitable for the proposal as well as it has potential to be used as an intracanal dressing since presented antimicrobial activity against all bacterial strains evaluated.

scholarly journals Electrospun Nanofibers/Nanofibrous Scaffolds Loaded with Silver Nanoparticles as Effective Antibacterial Wound Dressing Materials

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 964
Author(s):  
Sibusiso Alven ◽  
Buhle Buyana ◽  
Zizo Feketshane ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Mechanical Performance ◽  
Healing Process ◽  
High Surface ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
High Porosity ◽  
Nanofibrous Scaffolds

The treatment of wounds is expensive and challenging. Most of the available wound dressings are not effective and suffer from limitations such as poor antimicrobial activity, toxicity, inability to provide suitable moisture to the wound and poor mechanical performance. The use of inappropriate wound dressings can result in a delayed wound healing process. Nanosize range scaffolds have triggered great attention because of their attractive properties, which include their capability to deliver bioactive agents, high surface area, improved mechanical properties, mimic the extracellular matrix (ECM), and high porosity. Nanofibrous materials can be further encapsulated/loaded with metal-based nanoparticles to enhance their therapeutic outcomes in wound healing applications. The widely studied metal-based nanoparticles, silver nanoparticles exhibit good properties such as outstanding antibacterial activity, display antioxidant, and anti-inflammatory properties, support cell growth, making it an essential bioactive agent in wound dressings. This review article reports the biological (in vivo and in vitro) and mechanical outcomes of nanofibrous scaffolds loaded with silver nanoparticles on wound healing.

Sign in / Sign up

Export Citation Format

Share Document