scholarly journals Isolation of Phenolics from Rhizophora mangle by Combined Counter-current Chromatography and Gel-Filtration

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901
Author(s):  
Fernanda das Neves Costa ◽  
Marcos Daniel da Silva ◽  
Ricardo Moreira Borges ◽  
Gilda Guimarães Leitão
Keyword(s):  
Phenolic Compounds ◽  
Leaf Extract ◽  
Gel Filtration ◽  
Rhizophora Mangle ◽  
Solvent System ◽  
Hydroxybenzoic Acid ◽  
Etoac Extract ◽  
Counter Current ◽  
First Time ◽  
Tof Ms

Nine phenolic compounds, quercetin, epi-catechin, catechin, 4-hydroxybenzoic acid, kaempferol 3- O-β-glucopyranoside, quercetin 3- O-β-glucopyranoside, quercetin 3- O-6″- trans-coumaroyl-β-glucoside, kaempferol 3- O-β-rutinoside and quercetin 3- O-β-rutinoside, were isolated from the EtOAc leaf extract of Rhizophora mangle (Rhizophoraceae) combining counter-current chromatography (CCC) and gel-filtration. A solvent system of n-hexane-ethyl acetate-methanol-water (1.5:6:1.5:6) was employed at the preliminary stage of EtOAc extract fractionation as it was shown to contain compounds that differed highly in their hydrophobicity. The obtained fractions were further purified by either CCC or gel-filtration depending on their complexity. The isolated compounds were analyzed by NMR spectroscopy and the proposed structures were confirmed by HRES/ESI/TOF MS. Some of these compounds were isolated and/or identified for the first time in R. mangle.

Determination of Phenolic Compounds in the Methanolic Extract of Pourouma guianensis Leaves by UPLC-DAD-MS

2020 ◽  
Vol 10 (5) ◽  
pp. 566-570
Author(s):  
Queitilane de S. Sales ◽  
Marcelo da S. Mathias ◽  
Rodrigo R. de Oliveira
Keyword(s):  
Phenolic Compounds ◽  
Formic Acid ◽  
Solvent System ◽  
Efficient Tool ◽  
Phenolic Substances ◽  
Chemical Knowledge ◽  
First Time ◽  
Southeastern Region

Background: The presence of phenolic compounds is common in Urticaceae. However, only one report of the isolation of an apigenin in the Pourouma genus is available. Pourouma guianensis occurs widely throughout Brazil, mainly in the Southeastern region, and is popularly known as “pitinga”. The chemical profile of P. guianensis is marked by the presence of steroids and triterpenes. Objective: This study carried out the characterization of phenolic substances using UPLC-DAD-MS separation, in order to increase the chemical knowledge on the Pourouma genus. Methods: The extract was analyzed by UPLC-DAD-MS using a C-18 column, DAD detector at 190- 400nm and ESI-Q-TOF mass spectrometer. The solvent system consisted of water with 0.1% formic acid (phase A) and acetonitrile with 0.1% formic acid (phase B). Results: The extract analysis identified the presence of ten compounds: two aryl propanoids (5-Ocaffeoylquinic acid and 3-O-feruloylquinic acid) and eight glycosylated flavones derived from apigenin, luteonin, and chrysoeryol (vicenin 2, saponarin, orientin, isoorientin, isoschaftoside, schaftoside, isovitexin, and isoscoparin). Conclusion: The developed UPLC-DAD method was proven to be an efficient tool for the separation of phenolic mixtures and the UPLC-DAD-MS analysis enabled the identification of these compounds, reported for the first time in Pourouma genus.

Hypoglycemic activity of Origanum vulgare L. and its main chemical constituents identified with HPLC-ESI-QTOF-MS

2021 ◽  
Vol 12 (6) ◽  
pp. 2580-2590
Author(s):  
Huawei Yu ◽  
Panpan Zhang ◽  
Hongbing Liu ◽  
Xiaojuan Sun ◽  
Jichao Liang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Leaf Extract ◽  
Chemical Constituents ◽  
Lipid Synthesis ◽  
Signalling Pathway ◽  
Hypoglycemic Activity ◽  
Origanum Vulgare ◽  
Tof Ms ◽  
Stress Signalling

Origanum vulgare L. leaf extract showed promising hypoglycemic activity through gluconeogenesis, lipid synthesis and relieving oxidative stress signalling pathway, and its potential active phenolic compounds were also identified by HPLC-ESI-Q-TOF-MS method.

Isolation and Purification of Ginkgo Flavonoids by High-Speed Counter-Current Chromatography

2013 ◽  
Vol 781-784 ◽  
pp. 741-745
Author(s):  
Sang Sang Lu ◽  
Hui Song ◽  
Jing Zhi Miao ◽  
Shi Rong Tang
Keyword(s):  
Mobile Phase ◽  
High Speed ◽  
High Performance ◽  
Leaf Extract ◽  
Volume Ratio ◽  
Solvent System ◽  
Two Phase ◽  
Lower Phase ◽  
Isolation And Purification ◽  
Counter Current

High-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of Ginkgo flavonoids from the Ginkgo biloba L. leaf extract (GBE) with a two-phase solvent system composed of n-hexaneethyl acetatemethanolwater at an optimized volume ratio of 4:6:5:5(v/v/v/v). The lower phase was used as the mobile phase in the head to tail elution mode. The preparative HSCCC separation was performed on 200 mg of GBE yielding pure Quercetin (22mg), Kaempferol (15mg) and Isorhamnetin (4mg) at purities of 96.6%, 92.3% and 93.6%, respectively, as determined by high performance liquid chromatography (HPLC). HSCCC is a powerful technique for isolation and separation of chemical composition from GBE.

scholarly journals Emodin Isolation from the Leaves of Pteridium acquilinum

2019 ◽  
pp. 1-6
Author(s):  
M. E.. Khan ◽  
E. T. Williams ◽  
A. Abel
Keyword(s):  
Chemical Structure ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Solvent System ◽  
Female Rats ◽  
Leaf Extracts ◽  
Two Phase ◽  
Vacuum Liquid Chromatography ◽  
First Time

This study was designed to isolate and characterize the active compound(s) from the leaf extract of Pteridiumacquilinum after the aqueous and methanolic leaf extracts had been investigated on some female rats hormones One anthraquinoneemodin (1, 3, 8-trihydroxy-6-methyl-anthra-quinone) was successfully purified from the methanolic extract of the medicinal plant by Chromatography (VLC, TLC and Sephadex). The initial elution was with n hexane– ethyl acetate–methanol (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin. The Vacuum Liquid Chromatography (VLC), fraction (from the methanol extract) was analyzed by Nuclear magnetic resonance (NMR) and the chemical structure of the anthraquinone was confirmed by 1H-NMR and 13C-NMR analyses. This is the first time this anthraquinone, emodin is being reported from P. aquilinum.

Chemical Constituents from the Root and Stem of Zanthoxylum avicennae

2014 ◽  
Vol 618 ◽  
pp. 426-430 ◽  
Author(s):  
Tao Guo ◽  
Xiao Feng Tang ◽  
Ju Bao Zhang ◽  
Jun Qing Wei ◽  
Ya Wang ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Hydroxybenzoic Acid ◽  
Etoac Extract ◽  
Data Analyses ◽  
First Time

Objective: To study the chemical constituents from the root and stem of Zanthoxylum avicennae. Method: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. Result: Fourteen compounds were isolated from the EtOAc extract and identified as Avicennin (1), Xanthoxyletin (2), Luvangetin (3), Norchelerythrine (4), Diisobutyl phthalate (5), β-amyrin (6), Lupeol (7), Chelerythrine (8), Dictamine (9), γ-fagarine (10) and Skimmianine (11), Sesamin (12) Syringaresinol (13) and P-hydroxybenzoic acid (14). Conclusion: The compounds 2, 4, 5, 6, 7, 12 and 14 were obtained from this plant for the first time.

scholarly journals Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Principal Component ◽  
Total Content ◽  
Component Analysis ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

scholarly journals Facile Green, Room-Temperature Synthesis of Gold Nanoparticles Using Combretum erythrophyllum Leaf Extract: Antibacterial and Cell Viability Studies against Normal and Cancerous Cells

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 893
Author(s):  
Olufunto T. Fanoro ◽  
Sundararajan Parani ◽  
Rodney Maluleke ◽  
Thabang C. Lebepe ◽  
Jose R. Varghese ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Viability ◽  
Leaf Extract ◽  
Room Temperature ◽  
Klebsiella Oxytoca ◽  
Cost Effective ◽  
Average Diameter ◽  
Antibacterial Activities ◽  
Inhibition Concentration ◽  
First Time

We herein report a facile, green, cost-effective, plant-mediated synthesis of gold nanoparticles (AuNPs) for the first time using Combretum erythrophyllum (CE) plant leaves. The synthesis was conducted at room temperature using CE leaf extract serving as a reducing and capping agent. The as-synthesized AuNPs were found to be crystalline, well dispersed, and spherical in shape with an average diameter of 13.20 nm and an excellent stability of over 60 days. The AuNPs showed broad-spectrum antibacterial activities against both pathogenic Gram-positive (Staphylococcus epidermidis (ATCC14990), Staphylococcus aureus (ATCC 25923), Mycobacterium smegmatis (MC 215)) and Gram-negative bacteria (Proteus mirabilis (ATCC 7002), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13822), Klebsiella oxytoca (ATCC 8724)), with a minimum inhibition concentration of 62.5 µg/mL. In addition, the as-synthesized AuNPs were highly stable with exceptional cell viability towards normal cells (BHK- 21) and cancerous cancer cell lines (cervical and lung cancer).

scholarly journals Insight into the Secondary Metabolites of Geum urbanum L. and Geum rivale L. Seeds (Rosaceae)

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1219
Author(s):  
Marek Bunse ◽  
Peter Lorenz ◽  
Florian C. Stintzing ◽  
Dietmar R. Kammerer
Keyword(s):  
Fatty Acids ◽  
Phenolic Compounds ◽  
Unsaturated Fatty Acids ◽  
Asiatic Acid ◽  
Cosmetic Products ◽  
Nutritional Properties ◽  
First Time ◽  
Derivatives Of ◽  
Geum Urbanum ◽  
Insight Into

The present study aimed at the identification and quantitation of phenolic compounds, fatty acids, and further characteristic substances in the seeds of Geum urbanum L. and Geum rivale L. For this purpose, individual components of extracts recovered with MeOH, CH2Cl2, and by cold-pressing, respectively, were characterized by HPLC-DAD/ESI-MSn and GC/MS and compared with reference compounds. For both Geum species, phenolic compounds, such as flavonoids and gallic acid derivatives, and triterpenes, such as saponins and their aglycones, were detected. Surprisingly, both Geum species revealed the presence of derivatives of the triterpenoid aglycons asiatic acid and madecassic acid, which were characterized for the first time in the genus Geum. Furthermore, the fatty acids of both species were characterized by GC–MS after derivatization. Both species showed a promising fatty-acid profile in terms of nutritional properties because of high proportions of unsaturated fatty acids. Linoleic acid and linolenic acid were most abundant, among other compounds such as palmitic acid and stearic acid. In summary, the present study demonstrates the seeds of G. urbanum and G. rivale to be a valuable source of unsaturated fatty acids and bioactive phenolics, which might be exploited for nutritional and cosmetic products and for phytotherapeutic purposes.

scholarly journals Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Hot Air ◽  
Vis Spectroscopy ◽  
Pimenta Dioica ◽  
First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY GLYCYRRHIZA PALLIDIFLORA HERB (GLYCYRRHIZA PALLIDIFLORA MAXIM.)

2021 ◽  
pp. 42-46
Author(s):  
Maltseva E.M. ◽  
Egorova I.N. ◽  
Pinchuk L.G.
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Russian Far East ◽  
Far East ◽  
Biologically Active ◽  
Perennial Herb ◽  
Total Phenolic ◽  
Plant Antioxidants ◽  
Phytochemical Studies ◽  
First Time

Pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.) is the pea family (Fabaceae) perennial herb. It is a Russian Far East endemic. Pale-flowered licorice refers to the false (not sweet) licorice section (Pseudoglycyrrhiza Krug.), which do not accumulate glycyrrhizin derivatives. The G. pallidiflora successful introduction has been carried out over the past 5 years in the “Apothecary garden” territory, FRC UUH SB RAS, Kemerovo. Phytochemical studies screening biologically active compounds main classes of the plant aerial part confirmed the presence of a complex of phenolic compounds. The content of catechin derivatives and condensed-type tannins - proanthocyanidins (PAC) data in G. pallidiflora herb were obtained for the first time. In different years of cultivation, licorice herb accumulates phenolic compounds - up to 2.83 ± 0.22% in terms of gallic acid, flavonoids - up to 2.44 ± 0.03% in terms of rutin and PAC in terms of cyanidine chloride - up to 2.61 ± 0.11%. It was found that the maximum content of the phenolic compounds and PAC sum was observed in the herb for 4 years development. The greatest number of flavonoids accumulates in the herb harvested in the 3rd year of cultivation. A positive significant linear relation was found between antioxidant activity (AOA) and the total phenolic compounds and PAC content (r≥0.98). Considering that BAC with IC values ≤ 50 μg / ml in the DPPH assay refer to active antioxidant’s licorice herb can be classified as a plant with high antioxidant potential. It was found that the iron chelating activity of the G. pallidiflora herb is in direct dependence (r≥0.94) on the content of flavonoids. The obtained results demonstrate the importance of further study of this plant as a source of BAC, including plant antioxidants.

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