Subgingival Microbiota during Healthy Pregnancy and Pregnancy Gingivitis

2020 ◽  
pp. 238008442094877
Author(s):  
P. Balan ◽  
B.W. Brandt ◽  
Y.S. Chong ◽  
W. Crielaard ◽  
M.L. Wong ◽  
...  
Keyword(s):  
Oral Health ◽  
Pregnant Women ◽  
Oral Microbiome ◽  
Microbial Colonization ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Health Maintenance ◽  
Prenatal Health ◽  
Healthy Women ◽  
Healthy Pregnancy

Introduction: Previous studies have largely explored the microbial composition and pathogenesis of pregnancy gingivitis. However, the patterns of microbial colonization during pregnancy in the absence of pregnancy gingivitis have rarely been studied. Characterization of the oral microbiome in pregnant women with healthy gingiva is an important initial step in understanding the role of the microbiome in progression to pregnancy gingivitis. Objectives: In this study, we compared the oral microbiome of pregnant women without gingivitis (healthy pregnancy) with pregnant women having gingivitis and nonpregnant healthy women to understand how pregnancy modifies the oral microbiome and induces progression to pregnancy gingivitis. Methods: Subgingival plaque samples were collected from Chinese pregnant women with gingivitis ( n = 10), healthy pregnant women ( n = 10), and nonpregnant healthy women ( n = 10). The Illumina MiSeq platform was used to perform 16S rRNA gene sequencing targeting the V4 region. Results: The alpha and beta diversity was significantly different between pregnant and nonpregnant women, but minimal differences were observed between pregnant women with and without gingivitis. Interestingly, the oral bacterial community showed higher abundance of pathogenic taxa during healthy pregnancy as compared with nonpregnant women despite similar gingival and plaque index scores. However, when compared with overt pregnancy gingivitis, pathogenic taxa were less abundant during healthy pregnancy. PICRUSt analysis (phylogenetic investigation of communities by reconstruction of unobserved states) also suggested no difference in the functional capabilities of the microbiome during pregnancy, irrespective of gingival disease status. However, metabolic pathways related to amino acid metabolism were significantly increased in healthy pregnant women as compared with nonpregnant women. Conclusion: The presence of pathogenic taxa in healthy pregnancy and pregnancy gingivitis suggests that bacteria may be necessary for initiating disease development but progression to gingivitis may be influenced by the host environmental factors. More efforts are required to plan interventions aimed at sustaining health before the appearance of overt gingivitis. Knowledge Transfer Statement: The results of this study draw attention to the importance of oral health maintenance during pregnancy, as women without any prenatal oral conditions are predisposed to the risk of developing pregnancy gingivitis. Hence, it is important to incorporate comprehensive assessment of oral health in the prenatal health care schedules. Pregnant woman should be screened for oral risks, counseled on proper oral hygiene and expected oral changes, and referred for dental treatment, when necessary.

EFEKTIVITAS BOOKLET TERHADAP PENGETAHUAN DAN SIKAP PEMELIHARAAN KESEHATAN GIGI MULUT PADA IBU HAMIL

2019 ◽  
Vol 4 (1) ◽  
pp. 66
Author(s):  
Rahmaini Fitri
Keyword(s):  
Oral Health ◽  
Pregnant Women ◽  
The Body ◽  
Wilcoxon Test ◽  
Percentage Increase ◽  
Oral Diseases ◽  
Health Maintenance ◽  
Knowledge And Attitudes ◽  
Before And After ◽  
Knowledge And Attitude

Pregnancy causes physiological changes in the body and as well as in the oral cavity. Dental and oral diseases associated with pregnancy that is, gingivitis, periodontitis and pregnancy granuloma. Mouth dental disease during pregnancy is not only influenced by the pregnancy itself but rather the lack of knowledge about dental and oral health maintenance. Efforts to improve the knowledge of pregnant women about oral health is done by providing information, information necessary for health education media. In this case the media is created and used to improve the knowledge of pregnant women is the booklet. This study aimed to analyze the differences in knowledge and attitudes before and after maintenance booklet oral health in pregnant women. This study is a quasi experiment with one group pre  and post test design. The sample is the first trimester pregnant women who come to the health center in Sentosa Baru Medan as many as 34 people. Analysis of the data  forcompare the average difference in scores of knowledge and attitudes before and after the intervention used the Wilcoxon test. The results showed there is an increased knowledge and attitude maintenance of oral health in pregnant women after being given a booklet with a value of p < 0.001, a percentage increase of 30% knowledge and attitudes percentage of 37%. The conclusions of this study is increased knowledge and attitude maintenance of oral health in pregnant women after being given a booklet.

scholarly journals Association between changes in salivary microbiota with glucose levels during pregnancy – findings from a pilot study

2020 ◽  
Author(s):  
Xin Zhang ◽  
Pei Wang ◽  
Liangkun Ma ◽  
Rongjun Guo ◽  
Yongjing Zhang ◽  
...  
Keyword(s):  
Information Processing ◽  
Pregnant Women ◽  
Early Stage ◽  
Fasting Blood Glucose ◽  
Alpha Diversity ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Health Maintenance ◽  
Maternal Characteristics ◽  
Salivary Microbiota

Abstract Background : Microbial shifts that correspond to host variations during pregnancy are vital in health maintenance. Significant changes have been reported in the oral microbiota of pregnant women when compared with nonpregnant women, but little is known about the dynamic shifts in oral microbiota during the pregnancy course. Methods : In this study, changes in salivary microbiota in 81 healthy pregnant women throughout the early stage (G1: 9-14 weeks), middle stage (G2: 21-28 weeks), and late stage (G3: 31-38 weeks) were investigated with 16S rRNA sequencing techniques. Correlations between salivary microbiota and maternal characteristics, including fasting blood glucose (FBG) levels, were evaluated. Results : Alpha diversity indexes were stable throughout pregnancy, but significant changes were found in beta diversity measured by weighted and unweighted UniFrac distances. Fourteen dominant trimester-specific taxa were identified using the LEfSe method, including Bacteroidetes in G1, Proteobacteria in G2 and Firmicutes in G3 at the phylum level. Tax4Fun prediction analysis revealed significant changes in Genetic Information Processing, Environmental Information Processing, Unclassified and Human Diseases in G2 and in Metabolism in G3 when compared to G1. Significant correlations were found between FBG levels and microbial composition, and these correlations were independent of gestational diabetes mellitus (GDM) status. Conclusion : Within the limitations of this study, the dynamic changes in salivary microbiota during pregnancy were characterized, and beyond pregnancy, FBG was also involved in shaping the salivary microbiota.

scholarly journals Taxonomic Analysis of Oral Microbiome during Orthodontic Treatment

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Alessandra Campobasso ◽  
Eleonora Lo Muzio ◽  
Giovanni Battista ◽  
Domenico Ciavarella ◽  
Vito Crincoli ◽  
...  
Keyword(s):  
Orthodontic Treatment ◽  
Data Extraction ◽  
Human Study ◽  
Oral Microbiome ◽  
Orthodontic Appliances ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Fixed Appliance ◽  
Gene Search ◽  
Evidence Quality

Background. Orthodontic appliances induce significant changes in the oral microbiome, but this shift in microbial composition has not been well established by the available evidence yet. Objectives. To perform a systematic review of existing literature in order to assess the taxonomic microbial changes in orthodontic patients during Fixed Appliance Treatment (FAT) and Clear Aligner Treatment (CAT), using next-generation sequencing (NGS) technique of the bacterial 16S rRNA gene. Search Methods and Selection Criteria. The search for articles was carried out in PubMed, including articles published in English until May 2021. They included every human study report potentially relevant to the review. Data Collection and Analysis. After duplicate study selection and data extraction procedures according to the PICOS scheme, the methodological quality of the included papers was assessed by the Swedish Council on Technology Assessment in Health Care Criteria for Grading Assessed Studies (SBU) method. Results. The initial search identified 393 articles, 74 of which were selected by title and abstract. After full-text reading, six articles were selected according to inclusion criteria. The evidence quality for all the studies was moderate. Conclusions. Orthodontic treatment seems to transiently affect the composition of subgingival microbiome, although not salivary, maintaining a stable microbial diversity. Different results were found in the shift of microbiome between plaque and saliva, depending on the type of orthodontic treatment. This review should be interpreted with some caution because of the number, quality, and heterogeneity of the included studies.

scholarly journals Investigation of Oral Microbiome in Donkeys and the Effect of Dental Care on Oral Microbial Composition

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2245
Author(s):  
Yiping Zhu ◽  
Wuyan Jiang ◽  
Reed Holyoak ◽  
Bo Liu ◽  
Jing Li
Keyword(s):  
Dental Treatment ◽  
16S Rrna Gene Sequencing ◽  
Oral Microbiome ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Dental Procedures ◽  
Significant Difference ◽  
Dental Diseases ◽  
Rrna Gene Sequencing ◽  
The Impact

The objective of this study was to investigate the oral microbial composition of the donkey and whether basic dental treatment, such as dental floating, would make a difference to the oral microbial environment in donkeys with dental diseases using high-throughput bacterial 16S rRNA gene sequencing. Oral swab samples were collected from 14 donkeys with various dental abnormalities on day 0 (before treatment) and day 20 (twenty days after treatment). It is the first report focusing on the oral microbiome in donkeys with dental diseases and the impact of common dental procedures thereon. Identified in group Day 0 and group Day 20, respectively, were 60,439.6 and 58,579.1 operational taxonomic units (OTUs). Several taxa in Day 0 differed significantly from Day 20 at the phylum and genus levels, but no statistically significant difference was observed in richness and diversity of Day 0 and Day 20. The results also indicated that a larger-scale study focusing on healthy donkey oral microbiome, as well as the correlation of dental diseases and oral microbiomes at different time frames following more specific and consistent dental treatment, are warranted.

Pengaruh Pemeliharaan Kesehatan Mulut Ibu Hamil terhadap Status Tumbuh Kembang Janin

2021 ◽  
Vol 3 (2) ◽  
pp. 81-88
Author(s):  
Vina Dwi Wahyunita Vina ◽  
Sitti S. Hermanses
Keyword(s):  
Oral Health ◽  
Pregnant Women ◽  
Oral Hygiene ◽  
Growth And Development ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Average Growth ◽  
Health Maintenance ◽  
Quasi Experimental

Introduction: At Lorulun Public health Center, pregnant women who do Antenatal Care (ANC) visits with complaints around the mouth are still quite high (89.8%), and the implementation of health promotion for maintaining oral health during pregnancy is rarely carried out in a structured manner in conjunction with other pregnancy counseling activities. The aim to determine the effect of oral health maintenance in pregnant women on the growth and development of the fetus during pregnancy. Methods: This study is a quasi-experimental design with a non-equivalent control group design of two groups with a total sample of 64 respondents. Sampling was done using a purposive method. Data analysis of growth and development variable was carried out using independent t-test, oral hygiene variable was analyzed using Mann Whitney test and oral hygiene was analyzed using ANOVA test. Results: The results of this study showed that health education methods (video and demonstrations) were effective in increasing the average growth status of the fetus with TFU 28.06 (cm) TBJ 2334.69 grams and reducing the OHIS value of 1.26 (better oral hygiene), while oral hygiene was not affect the status of fetal growth and development. Conclusion: Oral hygiene does not affect the growth and development of the fetus, but it is hoped that pregnant women will continue to pay attention to their oral hygiene during pregnancy because oral hygiene affects the intake of nutritional needs that enter the fetus.

scholarly journals The Relationship Between Knowledge and Assessment of Pregnant Women with the Utilization of Dental Health Services

2020 ◽  
Vol 4 (6) ◽  
pp. 177-179
Author(s):  
Aprilia Martha Bakoil ◽  
Dinah Charlota Lerik ◽  
Sabina Gero
Keyword(s):  
Public Health ◽  
Oral Health ◽  
Health Services ◽  
Pregnant Women ◽  
Dental Health ◽  
Oral Disease ◽  
Health Maintenance ◽  
Dental Health Services ◽  
Oral Health Services ◽  
The Relationship

Improving the optimal health status requires commitment and continuous effort so that a sub-standard development goals (SDGS) program is formed, one of which aims to tackle health problems in pregnant women. Dental and oral disease during pregnancy is a public health problem. The incidence of dental and oral diseases during this period is due to a lack of knowledge about oral health maintenance. The low use of service facilities by the community is related to treatment seeking behavior and ill-health concepts from the community. The purpose of the study was to analyze the relationship of knowledge and assessment of pregnant women with the use of dental health services at the Dental Clinic at the Health Centers in Kupang City. The design of this research was cross-sectional. The samples were 250 respondents, selected using proportional random sampling technique. Data were collected using a questionnaire as an instrument, then analyzed using ordinal regression test. Based on the data analysis results, it could be concluded that there was relationship between knowledge and assessment of pregnant women with the utilization of dental and oral health services in Public Health Center in Kupang City. Keywords: knowledge; pregnant women; utilization; dental and oral health services

scholarly journals Site- and Taxa-Specific Disease-Associated Oral Microbial Structures Distinguish Inflammatory Bowel Diseases

2021 ◽  
Author(s):  
Hari K Somineni ◽  
Jordan H Weitzner ◽  
Suresh Venkateswaran ◽  
Anne Dodd ◽  
Jarod Prince ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Oral Microbiome ◽  
Spatial And Temporal Variation ◽  
Rrna Gene ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Healthy Control ◽  
Inflammatory Bowel ◽  
A Site

Abstract Background The gut and oral microbiome have independently been shown to be associated with inflammatory bowel disease (IBD). However, it is not known to what extent gut and oral microbial disease markers converge in terms of their composition in IBD. Further, the spatial and temporal variation within the oral microenvironments of IBD remain to be elucidated. Patients and Methods We used a prospectively recruited cohort of patients with IBD (n = 47) and unrelated healthy control patients (n = 18) to examine the spatial and temporal distribution of microbiota within the various oral microenvironments, represented by saliva, tongue, buccal mucosa, and plaque, and compared them with stool. Microbiome characterization was performed using 16S rRNA gene sequencing. Results The oral microbiome displayed IBD-associated dysbiosis, in a site- and taxa-specific manner. Plaque samples depicted a relatively severe degree of dysbiosis, and the disease-associated dysbiotic bacterial groups were predominantly the members of the phylum Firmicutes. Our 16S rRNA gene analyses show that oral microbiota can distinguish patients with IBD from healthy control patients, with salivary microbiota performing the best, closely matched by stool and other oral sites. Longitudinal profiles of microbial composition suggest that some taxa are more consistently perturbed than others, preferentially in a site-dependent fashion. Conclusions Collectively, these data indicate the potential of using oral microbial profiles in screening and monitoring patients with IBD. Furthermore, these results support the importance of spatial and longitudinal microbiome sampling to interpret disease-associated dysbiotic states and eventually to gain insights into disease pathogenesis.

scholarly journals Gender Variations in the Oral Microbiomes of Elderly Patients with Initial Periodontitis

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Jie Zhao ◽  
Ying-Hui Zhou ◽  
Ya-Qiong Zhao ◽  
Yao Feng ◽  
Fei Yan ◽  
...  
Keyword(s):  
Immune System ◽  
Elderly Patients ◽  
16S Rrna Gene Sequencing ◽  
Oral Microbiome ◽  
Rrna Gene ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Linear Discriminant ◽  
Immune System Diseases ◽  
Analytical Approaches

Periodontitis is a globally prevalent disease that imposes a functional and aesthetic burden on patients. The oral microbiome influences human health. The aim of this study was at assessing gender variation in the subgingival bacterial microbiome of elderly patients with initial periodontitis and to determine the causes of this variation. Twelve males and twenty females (range 50–68 years old) with initial periodontitis provided subgingival plaque samples. 16S rRNA gene sequencing, QIIME-based data processing, and statistical analyses were carried out using several different analytical approaches to detect differences in the oral microbiome between the two groups. Males had higher Chao1 index, observed species, and phylogenetic diversity whole tree values than females. Analysis of β-diversity indicated that the samples were reasonably divided by the gender. The linear discriminant analysis effect size showed that the most representative biomarkers were the genus Haemophilus in males, whereas the dominant bacteria in females were Campylobacter. Kyoto Encyclopedia of Genes and Genomes analysis showed that predicting changes in the female oral microbiota may be related to the immune system and immune system diseases are the main factor in males. These data suggest that gender may be a differentiating factor in the microbial composition of subgingival plaques in elderly patients with initial periodontitis. These results could deepen our understanding of the role of gender in the oral microbiota present during initial periodontitis.

scholarly journals Gut Microbiota Signatures in Gestational Anemia

2021 ◽  
Vol 11 ◽  
Author(s):  
Yan Long ◽  
Fang Liang ◽  
Ruochun Guo ◽  
Chunyan Zhu ◽  
Xueqin Zhao ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Pregnant Women ◽  
First Trimester ◽  
Shannon Index ◽  
Rrna Gene ◽  
Healthy Controls ◽  
Third Trimester ◽  
Microbial Composition ◽  
The Third ◽  
Α Diversity

Gestational diseases are associated with altered intestinal microbiota in pregnant women. Characterizing the gut microbiota of gestational anemia (GA) may describe a novel role of gut microbial abnormality in GA. In this study, we investigated differences in gut microbiota between GA patients and healthy pregnant women from the first trimester (n = 24 vs. 54) and the third trimester (n = 30 vs. 56) based on the 16S rRNA gene sequencing method. No statistically significant differences in α-diversity were identified between GA patients and controls in the first trimester of pregnancy, whereas the Shannon index and observed OTUs were significantly lower in GA patients than in healthy controls in the third trimester. Distance-based redundancy analysis revealed striking differences in microbial communities in the third trimester between GA patients and controls. Four genera were significantly different in relative abundance between GA patients and healthy controls, while 12 genera differentiated significantly between GA patients and healthy controls in the third trimester. At the operational taxonomic unit (OTU) level, 17 OTUs and 30 OTUs were identified to be different between GA patients and healthy controls in the first and third trimesters, respectively. Changes in gut microbial composition of GA patients suggest a potential relation with GA, and provide insights into the prediction and intervention of gestational anemia.

scholarly journals Power Play of Commensal Bacteria in the Buccal Cavity of Female Nile Tilapia

2021 ◽  
Vol 12 ◽  
Author(s):  
Yousri Abdelhafiz ◽  
Jorge M. O. Fernandes ◽  
Erika Stefani ◽  
Davide Albanese ◽  
Claudio Donati ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Buccal Cavity ◽  
Amplicon Sequencing ◽  
Oral Microbiome ◽  
Rrna Gene ◽  
Opportunistic Pathogens ◽  
Female Fish ◽  
Microbial Composition ◽  
Opportunistic Bacteria ◽  
Power Play

Fish are widely exposed to higher microbial loads compared to land and air animals. It is known that the microbiome plays an essential role in the health and development of the host. The oral microbiome is vital in females of different organisms, including the maternal mouthbrooding species such as Nile tilapia (Oreochromis niloticus). The present study reports for the first time the microbial composition in the buccal cavity of female and male Nile tilapia reared in a recirculating aquaculture system. Mucus samples were collected from the buccal cavity of 58 adult fish (∼1 kg), and 16S rRNA gene amplicon sequencing was used to profile the microbial communities in females and males. The analysis revealed that opportunistic pathogens such as Streptococcus sp. were less abundant in the female buccal cavity. The power play of certain bacteria such as Acinetobacter, Acidobacteria (GP4 and GP6), and Saccharibacteria that have known metabolic advantages was evident in females compared to males. Association networks inferred from relative abundances showed few microbe–microbe interactions of opportunistic pathogens in female fish. The findings of opportunistic bacteria and their interactions with other microbes will be valuable for improving Nile tilapia rearing practices. The presence of bacteria with specific functions in the buccal cavity of female fish points to their ability to create a protective microbial ecosystem for the offspring.

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