scholarly journals Association between changes in salivary microbiota with glucose levels during pregnancy – findings from a pilot study

2020 ◽  
Author(s):  
Xin Zhang ◽  
Pei Wang ◽  
Liangkun Ma ◽  
Rongjun Guo ◽  
Yongjing Zhang ◽  
...  
Keyword(s):  
Information Processing ◽  
Pregnant Women ◽  
Early Stage ◽  
Fasting Blood Glucose ◽  
Alpha Diversity ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Health Maintenance ◽  
Maternal Characteristics ◽  
Salivary Microbiota

Abstract Background : Microbial shifts that correspond to host variations during pregnancy are vital in health maintenance. Significant changes have been reported in the oral microbiota of pregnant women when compared with nonpregnant women, but little is known about the dynamic shifts in oral microbiota during the pregnancy course. Methods : In this study, changes in salivary microbiota in 81 healthy pregnant women throughout the early stage (G1: 9-14 weeks), middle stage (G2: 21-28 weeks), and late stage (G3: 31-38 weeks) were investigated with 16S rRNA sequencing techniques. Correlations between salivary microbiota and maternal characteristics, including fasting blood glucose (FBG) levels, were evaluated. Results : Alpha diversity indexes were stable throughout pregnancy, but significant changes were found in beta diversity measured by weighted and unweighted UniFrac distances. Fourteen dominant trimester-specific taxa were identified using the LEfSe method, including Bacteroidetes in G1, Proteobacteria in G2 and Firmicutes in G3 at the phylum level. Tax4Fun prediction analysis revealed significant changes in Genetic Information Processing, Environmental Information Processing, Unclassified and Human Diseases in G2 and in Metabolism in G3 when compared to G1. Significant correlations were found between FBG levels and microbial composition, and these correlations were independent of gestational diabetes mellitus (GDM) status. Conclusion : Within the limitations of this study, the dynamic changes in salivary microbiota during pregnancy were characterized, and beyond pregnancy, FBG was also involved in shaping the salivary microbiota.

scholarly journals Modulation of Saliva Microbiota through Prebiotic Intervention in HIV-Infected Individuals

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1346 ◽  
Author(s):  
Nuria Jiménez-Hernández ◽  
Sergio Serrano-Villar ◽  
Alba Domingo ◽  
Xavier Pons ◽  
Alejandro Artacho ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Alpha Diversity ◽  
Rrna Gene ◽  
Oral Microbiota ◽  
Microbiota Composition ◽  
Hiv Status ◽  
T Helper ◽  
Immunodeficiency Virus ◽  
Salivary Microbiota ◽  
Rrna Gene Sequencing

Human immunodeficiency virus (HIV) infection is characterized by an early depletion of the mucosal associated T helper (CD4+) cells that impair the host immunity and impact the oral and gut microbiomes. Although, the HIV-associated gut microbiota was studied in depth, few works addressed the dysbiosis of oral microbiota in HIV infection and, to our knowledge, no studies on intervention with prebiotics were performed. We studied the effect of a six-week-long prebiotic administration on the salivary microbiota in HIV patients and healthy subjects. Also, the co-occurrence of saliva microorganisms in the fecal bacteria community was explored. We assessed salivary and feces microbiota composition using deep 16S ribosomal RNA (rRNA) gene sequencing with Illumina methodology. At baseline, the different groups shared the same most abundant genera, but the HIV status had an impact on the saliva microbiota composition and diversity parameters. After the intervention with prebiotics, we found a drastic decrease in alpha diversity parameters, as well as a change of beta diversity, without a clear directionality toward a healthy microbiota. Interestingly, we found a differential response to the prebiotics, depending on the initial microbiota. On the basis of 100% identity clustering, we detected saliva sequences in the feces datasets, suggesting a drag of microorganisms from the upper to the lower gastrointestinal tract.

scholarly journals Associations of the oral microbiota and Candida with taste, smell, appetite and undernutrition in older adults

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kristina S. Fluitman ◽  
Tim J. van den Broek ◽  
Max Nieuwdorp ◽  
Marjolein Visser ◽  
Richard G. IJzerman ◽  
...  
Keyword(s):  
Older Adults ◽  
Alpha Diversity ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Cross Sectional ◽  
Poor Appetite ◽  
Identification Score ◽  
Smell Function ◽  
Taste And Smell ◽  
Smell Identification

AbstractPoor taste and smell function are widely thought to contribute to the development of poor appetite and undernutrition in older adults. It has been hypothesized that the oral microbiota play a role as well, but evidence is scarce. In a cross-sectional cohort of 356 older adults, we performed taste and smell tests, collected anthropometric measurements and tongue swabs for analysis of microbial composition (16S rRNA sequencing) and Candida albicans abundance (qPCR). Older age, edentation, poor smell and poor appetite were associated with lower alpha diversity and explained a significant amount of beta diversity. Moreover, a lower Streptococcus salivarius abundance was associated with poor smell identification score, whereas high C. albicans abundance seemed to be associated with poor smell discrimination score. In our population, neither the tongue microbiota, nor C. albicans were associated with poor taste or directly with undernutrition. Our findings do suggest a host-microbe interaction with regard to smell perception and appetite.

Subgingival Microbiota during Healthy Pregnancy and Pregnancy Gingivitis

2020 ◽  
pp. 238008442094877
Author(s):  
P. Balan ◽  
B.W. Brandt ◽  
Y.S. Chong ◽  
W. Crielaard ◽  
M.L. Wong ◽  
...  
Keyword(s):  
Oral Health ◽  
Pregnant Women ◽  
Oral Microbiome ◽  
Microbial Colonization ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Health Maintenance ◽  
Prenatal Health ◽  
Healthy Women ◽  
Healthy Pregnancy

Introduction: Previous studies have largely explored the microbial composition and pathogenesis of pregnancy gingivitis. However, the patterns of microbial colonization during pregnancy in the absence of pregnancy gingivitis have rarely been studied. Characterization of the oral microbiome in pregnant women with healthy gingiva is an important initial step in understanding the role of the microbiome in progression to pregnancy gingivitis. Objectives: In this study, we compared the oral microbiome of pregnant women without gingivitis (healthy pregnancy) with pregnant women having gingivitis and nonpregnant healthy women to understand how pregnancy modifies the oral microbiome and induces progression to pregnancy gingivitis. Methods: Subgingival plaque samples were collected from Chinese pregnant women with gingivitis ( n = 10), healthy pregnant women ( n = 10), and nonpregnant healthy women ( n = 10). The Illumina MiSeq platform was used to perform 16S rRNA gene sequencing targeting the V4 region. Results: The alpha and beta diversity was significantly different between pregnant and nonpregnant women, but minimal differences were observed between pregnant women with and without gingivitis. Interestingly, the oral bacterial community showed higher abundance of pathogenic taxa during healthy pregnancy as compared with nonpregnant women despite similar gingival and plaque index scores. However, when compared with overt pregnancy gingivitis, pathogenic taxa were less abundant during healthy pregnancy. PICRUSt analysis (phylogenetic investigation of communities by reconstruction of unobserved states) also suggested no difference in the functional capabilities of the microbiome during pregnancy, irrespective of gingival disease status. However, metabolic pathways related to amino acid metabolism were significantly increased in healthy pregnant women as compared with nonpregnant women. Conclusion: The presence of pathogenic taxa in healthy pregnancy and pregnancy gingivitis suggests that bacteria may be necessary for initiating disease development but progression to gingivitis may be influenced by the host environmental factors. More efforts are required to plan interventions aimed at sustaining health before the appearance of overt gingivitis. Knowledge Transfer Statement: The results of this study draw attention to the importance of oral health maintenance during pregnancy, as women without any prenatal oral conditions are predisposed to the risk of developing pregnancy gingivitis. Hence, it is important to incorporate comprehensive assessment of oral health in the prenatal health care schedules. Pregnant woman should be screened for oral risks, counseled on proper oral hygiene and expected oral changes, and referred for dental treatment, when necessary.

Caries-associated Salivary Microbiota of Children at Mixed Dentition from Different Geographic Locations

2021 ◽  
Author(s):  
Shanshan Li ◽  
Shi Huang ◽  
Ying Zhang ◽  
Lijuan Zhang ◽  
Fan Li ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Alpha Diversity ◽  
Mixed Dentition ◽  
Diagnostic Model ◽  
Human Populations ◽  
Healthy Children ◽  
Microbial Composition ◽  
Model Based ◽  
Geographical Regions ◽  
Salivary Microbiota

Abstract The microbial composition of dental caries may depend on age, diet, and geography, yet the effect of geography on these microbiomes is largely underexplored. Here, we profiled and compared saliva microbiota from 130 individuals aged 6 to 8 years old, representing both healthy children (H) and children with severe caries (C) from two geographical regions of China: Qingdao Guangzhou. First, the saliva microbiota exhibited profound differences in diversity and composition between the C and H groups. The caries microbiota featured a lower alpha diversity and more variable community structure than the healthy microbiota. Furthermore, the relative abundance of several genera (e.g., Lactobacillus, Gemella and Cryptobacterium) was significantly higher in the C group than in the H group. Next, geography dominated over disease status in shaping salivary microbiota, and a wide array of salivary bacteria was highly predictive of the individuals’ city of origin. Finally, we built a universal diagnostic model based on 14 bacterial species, which can diagnose caries with 87% and 85% accuracy within each city and 83% accuracy across cities. These findings demonstrated that despite the large effect size of geography, a universal model based on salivary microbiota has the potential to diagnose caries across human populations.

scholarly journals Microbiome Analysis Reveals the Dynamic Alternations in Gut Microbiota of Diarrheal Giraffa camelopardalis

2021 ◽  
Vol 8 ◽  
Author(s):  
Li Xi ◽  
Yumin Song ◽  
Xinxi Qin ◽  
Jincheng Han ◽  
Yung-Fu Chang
Keyword(s):  
Gut Microbiota ◽  
Relative Abundance ◽  
Alpha Diversity ◽  
Microbial Composition ◽  
Health States ◽  
Giraffa Camelopardalis ◽  
Health Maintenance ◽  
Bacterial Phyla ◽  
Gastrointestinal Health ◽  
Gut Microbial Community

The ruminant gut microbial community's importance has been widely acknowledged due to its positive roles in physiology, metabolism, and health maintenance. Diarrhea has been demonstrated to cause adverse effects on gastrointestinal health and intestinal microecosystem, but studies regarding diarrheal influence on gut microbiota in Giraffa camelopardalis have been insufficient to date. Here, this study was performed to investigate and compare gut microbial composition and variability between healthy and diarrheic G. camelopardalis. The results showed that the gut microbial community of diarrheal G. camelopardalis displayed a significant decrease in alpha diversity, accompanied by distinct alterations in taxonomic compositions. Bacterial taxonomic analysis indicated that the dominant bacterial phyla (Proteobacteria, Bacteroidetes, and Firmicutes) and genera (Escherichia Shigella and Acinetobacter) of both groups were the same but different in relative abundance. Specifically, the proportion of Proteobacteria in the diarrheal G. camelopardalis was increased as compared with healthy populations, whereas Bacteroidetes, Firmicutes, Tenericutes, and Spirochaetes were significantly decreased. Moreover, the relative abundance of one bacterial genus (Comamonas) dramatically increased in diarrheic G. camelopardalis, whereas the relative richness of 18 bacterial genera decreased compared with healthy populations. Among them, two bacterial genera (Ruminiclostridium_5 and Blautia) cannot be detected in the gut bacterial community of diarrheal G. camelopardalis. In summary, this study demonstrated that diarrhea could significantly change the gut microbial composition and diversity in G. camelopardalis by increasing the proportion of pathogenic to beneficial bacteria. Moreover, this study first characterized the distribution of gut microbial communities in G. camelopardalis with different health states. It contributed to providing a theoretical basis for establishing a prevention and treatment system for G. camelopardalis diarrhea.

scholarly journals Changes in oral microbiota after the initiation of chemotherapy in patients with hematopoietic tumors

2021 ◽  
Author(s):  
Michi Omori ◽  
Kato-kogoe Nahoko ◽  
Shoichi Sakaguchi ◽  
Eri Komori ◽  
Kazuya Inoue ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Alpha Diversity ◽  
Size Analysis ◽  
Oral Microbiota ◽  
Linear Discriminant ◽  
Chemotherapy Group ◽  
Salivary Microbiota ◽  
Before And After ◽  
Unweighted Unifrac ◽  
Induced Changes

Abstract Background Recently, the gut microbiota has been shown to play an important role in the response and resistance to chemotherapy. Although there is much knowledge about chemotherapy-induced changes in the gut microbiota, chemotherapy-associated changes in the oral microbiota remain unclear. Herein, we aimed to evaluate the changes in oral microbiota associated with the initiation of chemotherapy in patients with malignant hematopoietic tumors. Methods Oral samples were collected before and 8–20 days after the start of chemotherapy from 50 patients with malignant hematopoietic tumors who were starting chemotherapy for the first time. The 16S ribosomal RNA gene sequencing of bacterial DNA extracted from oral samples was performed to compare the oral microbiota before and after the initiation of chemotherapy. Results The richness or evenness of diversity in the ‘after start of chemotherapy’ group decreased significantly, compared with the ‘before start of chemotherapy’ group (alpha-diversity; observed operational taxonomic units (OTUs) index, p < 0.001; and Shannon’s index, p < 0.001). The overall salivary microbiota structure between the pre- and post-chemotherapy groups differed significantly (beta-diversity; unweighted UniFrac distances, p = 0.001; and weighted UniFrac distances, p = 0.003). Linear discriminant analysis effect size analysis demonstrated an increased abundance of species of certain genera, such as Staphylococcus, and decreased abundance of species of some genera, such as Streptococcus and Neisseria, in the ‘after-chemotherapy’ group, compared with those in the ‘before-chemotherapy’ group. The amounts and trends of change in the oral microbiota before and after the start of chemotherapy differed among the subjects. Of the 25 bacterial genera whose prevalence changed significantly before and after the start of chemotherapy, the proportion of oral commensals such as Streptococcus and Neisseria decreased in many subjects. In contrast, Staphylococcus and Pseudomonas were detected only in a few subjects, but their relative abundance increased significantly after the start of chemotherapy. Conclusions The oral microbiota of patients with hematopoietic tumors changed markedly after the initiation of chemotherapy. Our findings are expected to aid the elucidation of the pathogenesis of oral mucositis, which is an adverse event of chemotherapy, and the development of treatment methods for this condition.

scholarly journals The Salivary Microbiome: Analysis of by Pyrosequencing and the Relationship with Helicobacter pylori Infection

2018 ◽  
Author(s):  
Yingjie Ji ◽  
Xiao Liang ◽  
Hong Lu
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Bacterial Diversity ◽  
Alpha Diversity ◽  
Oral Microbiota ◽  
Operational Taxonomic Units ◽  
Hypervariable Regions ◽  
Salivary Microbiota ◽  
Before And After ◽  
H Pylori

Backgrounds: There have been reports of Helicobacter pylori (H. pylori) in the oral cavity and it has been suggested that the oral cavity may be a reservoir for H. pylori reflux from the stomach. Objectives: High-throughput pyrosequencing was used to assess the structure and composition of oral microbiota communities in individuals with or without confirmed H. pylori infection. Methods: Saliva samples were obtained from 34 H. pylori infected and 24 H. pylori uninfected subjects. Bacterial genomic DNA was extracted and examined by pyrosequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatics analysis. Saliva sampling was repeated from 22 of the 34 H. pylori infected subjects 2 months after H. pylori eradication. Results: High-quality sequences (2,812,659) clustered into 95,812 operational taxonomic units (OTUs; 97% identity), representing 440 independent species belonging to 138 genera, 68 families, 36 orders, 21 classes, and 11 phyla. Species richness (alpha diversity) of H. pylori infected subjects was similar to that of uninfected subjects. Eradication treatment decreased saliva bacterial diversity. Beta diversity analysis showed that the salivary microbial community structure differed between H. pylori infected and uninfected subjects both before and after H. pylori eradication. Conclusions: Salivary microbiota diversity was similar in H. pylori infected and uninfected individuals. Antibiotic therapy was associated with a decline in salivary bacterial diversity. Both H. pylori infection and its eradication caused the oral microbiota alterations in community and structure. The present of H. pylori in oral cavity was not related with its infection status in stomach.

The impact of gut microbial composition on response to neoadjuvant chemotherapy (NACT) in early-stage triple negative breast cancer (TNBC).

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. 590-590
Author(s):  
Nour Abuhadra ◽  
Chia-Chi Chang ◽  
Clinton Yam ◽  
Jason B White ◽  
Elizabeth Ravenberg ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Early Stage ◽  
Univariate Analysis ◽  
Alpha Diversity ◽  
Microbial Composition ◽  
Fecal Microbiota Transplant ◽  
Fecal Samples ◽  
Response To Chemotherapy ◽  
Significant Difference ◽  
The Impact

590 Background: The impact of gut microbiome on tumor biology, progression and response to immunotherapy has been shown across cancer types. However, there is little known about the impact of gut microbial composition on response to chemotherapy. We have previously shown that the gut microbiome remains unaltered during NACT in a cohort of 32 patients. Here we investigate the association between gut microbiome and response to NACT in a larger cohort of early-stage TNBC. Methods: Longitudinal fecal samples were collected from 85 patients with newly-diagnosed, early-stage TNBC patients enrolled in the ARTEMIS trial (NCT02276443). Patients all received standard NACT with adriamycin/cyclophosphamide (AC); volumetric change was assessed using ultrasound and patients with < 70% volumetric reduction (VR) after 4 cycles of AC were recommended to receive targeted therapy in addition to standard NACT to improve response rates. We performed 16S sequencing on bacterial genomic DNA extracted from 85 pre-AC fecal samples using the 2x250 bp paired-end read protocol. Quality-filtered sequences were clustered into Operational Taxonomic Units and classified using Mothur method with the Silva database version 138. For differential taxa-based univariate analysis, abundant microbiome taxa at species, genus, family, class, and order levels were analyzed using DESeq2 after logit transformation. Alpha-diversity indices within group categories were calculated using phyloseq. Microbial alpha diversity (within-sample diversity) was measured by Simpson's reciprocal index. β-diversity was measured using weighted UniFrac distances between the groups. The association between microbiota abundance and pathologic complete response (pCR) or residual disease (RD) was assessed using DESeq2 analysis. Results: Pre-AC fecal samples from 85 patients were available for analysis. Amongst them, there were 46 patients with pCR and 39 patients with RD. There was no significant difference in alpha diversity (p = 0.8) or beta-diversity (p = 0.7) between the pCR and RD groups. However, relative to patients with RD, the gut microbiome in patients with pCR was enriched for the Bifidobacterium longum species (p = 0.03). The gut microbiome in patients with RD was enriched for Lachnospiraceae (p = 0.03) at the genus level and the Bacteroides thetaiotaomicron species (p = 0.02). Conclusions: We have demonstrated significant differences in the gut microbial composition in patients with pCR as compared to patients with RD. Further investigation in larger studies is needed to support therapeutic exploration of gut microbiome modulation in TNBC patients receiving chemotherapy such as probiotic supplementation or fecal microbiota transplant.

scholarly journals Biocultural Drivers of Salivary Microbiota in Australian Aboriginal and Torres Strait Islander Children

2021 ◽  
Vol 2 ◽  
Author(s):  
Matilda Handsley-Davis ◽  
Emily Skelly ◽  
Newell W. Johnson ◽  
Kostas Kapellas ◽  
Ratilal Lalloo ◽  
...  
Keyword(s):  
Dental Caries ◽  
Torres Strait Islander ◽  
Oral Microbiota ◽  
Caries Risk ◽  
Management Options ◽  
Microbial Composition ◽  
Australian Aboriginal ◽  
Salivary Microbiota ◽  
Salivary Ph ◽  
Torres Strait

Australian Aboriginal and Torres Strait Islander children experience unacceptably high rates of dental caries compared to their non-Indigenous Australian counterparts. Dental caries significantly impacts the quality of life of children and their families, particularly in remote communities. While many socioeconomic and lifestyle factors impact caries risk, the central role of the oral microbiota in mediating dental caries has not been extensively investigated in these communities. Here, we examine factors that shape diversity and composition of the salivary microbiota in Aboriginal and Torres Strait Islander children and adolescents living in the remote Northern Peninsula Area (NPA) of Far North Queensland. We employed 16S ribosomal RNA amplicon sequencing to profile bacteria present in saliva collected from 205 individuals aged 4–17 years from the NPA. Higher average microbial diversity was generally linked to increased age and salivary pH, less frequent toothbrushing, and proxies for lower socioeconomic status (SES). Differences in microbial composition were significantly related to age, salivary pH, SES proxies, and active dental caries. Notably, a feature classified as Streptococcus sobrinus increased in abundance in children who reported less frequent tooth brushing. A specific Veillonella feature was associated with caries presence, while features classified as Actinobacillus/Haemophilus and Leptotrichia were associated with absence of caries; a Lactobacillus gasseri feature increased in abundance in severe caries. Finally, we statistically assessed the interplay between dental caries and caries risk factors in shaping the oral microbiota. These data provide a detailed understanding of biological, behavioral, and socioeconomic factors that shape the oral microbiota and may underpin caries development in this group. This information can be used in the future to improve tailored caries prevention and management options for Australian Aboriginal and Torres Strait Islander children and communities.

scholarly journals Salivary microbiomes of indigenous Tsimane mothers and infants are distinct despite frequent premastication

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2660 ◽  
Author(s):  
Cliff S. Han ◽  
Melanie Ann Martin ◽  
Armand E.K. Dichosa ◽  
Ashlynn R. Daughton ◽  
Seth Frietze ◽  
...  
Keyword(s):  
Preliminary Evidence ◽  
Food Samples ◽  
Feeding Practice ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Child Feeding ◽  
Alpha And Beta Diversity ◽  
Young Child Feeding ◽  
Salivary Microbiota ◽  
Oral Pathogens

BackgroundPremastication, the transfer of pre-chewed food, is a common infant and young child feeding practice among the Tsimane, forager-horticulturalists living in the Bolivian Amazon. Research conducted primarily with Western populations has shown that infants harbor distinct oral microbiota from their mothers. Premastication, which is less common in these populations, may influence the colonization and maturation of infant oral microbiota, including via transmission of oral pathogens. We collected premasticated food and saliva samples from Tsimane mothers and infants (9–24 months of age) to test for evidence of bacterial transmission in premasticated foods and overlap in maternal and infant salivary microbiota. We extracted bacterial DNA from two premasticated food samples and 12 matched salivary samples from maternal-infant pairs. DNA sequencing was performed with MiSeq (Illumina). We evaluated maternal and infant microbial composition in terms of relative abundance of specific taxa, alpha and beta diversity, and dissimilarity distances.ResultsThe bacteria in saliva and premasticated food were mapped to 19 phyla and 400 genera and were dominated by Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. The oral microbial communities of Tsimane mothers and infants who frequently share premasticated food were well-separated in a non-metric multi-dimensional scaling ordination (NMDS) plot. Infant microbiotas clustered together, with weighted Unifrac distances significantly differing between mothers and infants. Infant saliva contained more Firmicutes (p < 0.01) and fewer Proteobacteria (p < 0.05) than did maternal saliva. Many genera previously associated with dental and periodontal infections, e.g. Neisseria,Gemella,Rothia,Actinomyces,Fusobacterium, andLeptotrichia, were more abundant in mothers than in infants.ConclusionsSalivary microbiota of Tsimane infants and young children up to two years of age do not appear closely related to those of their mothers, despite frequent premastication and preliminary evidence that maternal bacteria is transmitted to premasticated foods. Infant physiology and diet may constrain colonization by maternal bacteria, including several oral pathogens.

Sign in / Sign up

Export Citation Format

Share Document