Gut micobiota alteration by Lactobacillus rhamnosus reduces pro-inflammatory cytokines and glucose level in the adult model of Zebrafish

Abstract Objective Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient’s circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology. Results In this trial 3-month old male adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After 5 days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p = 0.0001). These results suggest that probiotic intervention decreased the blood glucose level in the T2DM-P group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.

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Gut Micobiota Alteration by Lactobacillus Rhamnosus Reduces Pro-inflammatory Cytokines and Glucose Level in the Adult Model of Zebrafish

10.21203/rs.3.rs-505931/v1 ◽

2021 ◽

Author(s):

Fatemeh Bootorabi ◽

Farshid Saadat ◽

Reza Falak ◽

Hamed Manouchehri ◽

Reza Changizi ◽

...

Keyword(s):

Blood Glucose ◽

Glucose Level ◽

Inflammatory Cytokines ◽

Lactobacillus Rhamnosus ◽

Control Group ◽

Therapeutic Modalities ◽

Probiotic Treatment ◽

Probiotic Intervention ◽

Glucose Accumulation ◽

Pro Inflammatory Cytokines

Abstract Objective: Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient’s circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology.Results: In this trial three-month old mail adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After five days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p=0.0001). This results suggest that probiotic intervention hindered the blood glucose level in the T2DM group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.

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Anti-diabetic activity of diphenhydramine in diabetic rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3102 ◽

2020 ◽

Vol 11 (4) ◽

pp. 5067-5070

Author(s):

Pang Jyh Chayng ◽

Nurul Ain ◽

Kaswandi Md Ambia ◽

Rahim Md Noah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Insulin Level ◽

Diabetic Rats ◽

Group Iv ◽

Diabetic Rat ◽

Control Group ◽

Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p<0.05) and group IV (11.34 ±3.67, p<0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p<0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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Effects of Onchung-eum, an Herbal Prescription, on 5-Fluorouracil–Induced Oral Mucositis

Integrative Cancer Therapies ◽

10.1177/1534735418805560 ◽

2018 ◽

Vol 17 (4) ◽

pp. 1285-1296

Author(s):

Jae-Woo Park ◽

Jayoung Oh ◽

Seok-Jae Ko ◽

Mun Seog Chang ◽

Jinsung Kim

Keyword(s):

Inflammatory Cytokines ◽

Oral Mucositis ◽

Caspase 3 ◽

Skin Diseases ◽

Nuclear Factor Κb ◽

Control Group ◽

Nuclear Factor ◽

Cheek Pouches ◽

Herbal Prescription ◽

Pro Inflammatory Cytokines

In most cancer patients, chemotherapy-induced oral mucositis (OM) is a frequent side effect, leading to low quality of life and delay in therapy. The aim of this study was to evaluate the effects of Onchung-eum, a well-known herbal prescription in traditional medicine comprising 8 herbs that has long been used for skin diseases, on 5-fluorouracil (5-FU)–induced OM in human pharyngeal cells and golden Syrian hamsters. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and reactive oxygen species production were measured in vitro. The effects of Onchung-eum on OM of hamster cheek pouches induced by 5-FU were evaluated histologically and using TUNEL assay. In addition, the expression of nuclear factor-κB, caspase-3, and pro-inflammatory cytokines were measured by immunoblotting and immunohistochemistry. Significantly increased cell viability was observed in the Onchung-eum–treated groups compared with the 5-FU–treated control group. In 500 and 1000 mg/kg Onchung-eum–treated groups, the damaged epithelial layers in the cheek pouches of hamsters were significantly recovered. Moreover, at all concentrations, cell death in the cheek pouches of hamsters in the Onchung-eum–treated groups significantly decreased. The expression of pro-inflammatory cytokines, nuclear factor-κB, and caspase-3 also significantly decreased in Onchung-eum–treated groups at 500 and 1000 mg/kg. In conclusion, this study revealed that Onchung-eum can be used to treat chemotherapy-induced OM. However, further studies are required to understand the underlying mechanisms.

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Analysis of the physical activity effects and measurement of pro-inflammatory cytokines in irradiated lungs in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502012000300004 ◽

2012 ◽

Vol 27 (3) ◽

pp. 223-230 ◽

Cited By ~ 1

Author(s):

Renata Cristiane Gennari Bianchi ◽

Eduardo Rochete Ropelle ◽

Carlos Kiyoshi Katashima ◽

José Barreto Campello Carvalheira ◽

Luiz Roberto Lopes ◽

...

Keyword(s):

Physical Activity ◽

Western Blotting ◽

Inflammatory Cytokines ◽

Lower Lobe ◽

Whole Body ◽

Control Group ◽

Western Blotting Analysis ◽

Blotting Analysis ◽

Tnf Α ◽

Pro Inflammatory Cytokines

PURPOSE: To study if the pre-radiotherapy physical activity has radio-protective elements, by measuring the radio-induced activation of pro-inflammatory cytokines as interleukin-6 (il-6), transforming growth factor -β (tgf -β), tumor necrosis factor -α (tnf-α) and protein beta kinase β (ikkβ), through western blotting analysis. METHODS: A randomized study with 28 Wistar hannover rats, males, with a mean age of 90 days and weighing about 200 grams. The animals were divided into three groups: (GI, GII and GIII). GIII group were submitted to swimming for eight weeks (zero load, three times a week, about 30 minutes). Then, the groups (except the control group) were submitted to irradiation by cobalt therapy, single dose of 3.5 gray in the whole body. All animals were sacrificed by overdose of pentobarbital, according to the time for analysis of cytokines, and then a fragment of the lower lobe of the right lung went to western blotting analysis. RESULTS: The cytokines IKK β, TNF-α and IL-6 induced by radiation in the lung were lower in the exercised animals. However, exercise did not alter the radiation-induced increase in tgf-β. CONCLUSION: The results show a lower response in relation to inflammatory cytokines in the group that practiced the exercise pre-radiotherapy, showing that exercise can protect tissues from tissue damage due to irradiation.

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Associations between HBD3 and Pro-Inflammatory Cytokines in Asymptomatic Irreversible Pulpitis

Edelweiss Applied Science and Technology ◽

10.33805/2572-6978.105 ◽

2017 ◽

pp. 14-19

Author(s):

Anita Aminoshariae ◽

Mohammed Bakkar ◽

Tracey Bonfield ◽

Santosh Ghosh ◽

Thomas A Montagnese ◽

...

Keyword(s):

Inflammatory Cytokines ◽

Normal Group ◽

Control Group ◽

Spearman Correlation ◽

Whitney Test ◽

Cytokines And Chemokines ◽

Luminex Technology ◽

Spearman Correlation Test ◽

Irreversible Pulpitis ◽

Pro Inflammatory Cytokines

Objective: The aim of this study was to investigate the levels of Human Beta Defensin (hBD) 2 and 3, chemokine and cytokine expressions between teeth endodontically diagnosed with symptomatic irreversible pulpitis (SIP), asymptomatic irreversible pulpitis (ASIP) and normal pulps. We hypothesized that there would be a correlation between hBD’s and the immunoregulatory response. Design: Pulpal samples were collected with paper points. Six samples were obtained from normal teeth, 21 from SIP, 18 from ASIP. Levels of cytokines and betadefensins were measured by Luminex technology and ELISA, respectively. Data were statistically analyzed using Kruskal-Wallis, Wilcoxon Mann-Whitney test and Spearman correlation test. Differences were considered significant at p<0.05. Results: hBD-2 levels correlated with samples obtained from patients in the ASIP group, but not in the samples obtained from patients with SIP or the control group. HBD-3 concentrations associated with all of the cytokines and chemokines in both SIP and ASIP groups. However, in the normal group, hBD-3 correlated with only TNFα, IL-8, MCP-1, IL-1β, MIP-1a, RANTES, IL-17 in normal group. When comparing control levels of hBD-2 and hBD-3 with patients samples from either the ASIP or the SIP groups, hBD-2 and hBD-3 concentrations were highest in the ASIP group. Conclusions: The hBD-2 and-3 were highly associated with the levels of the chemokines and cytokines in ASIP group. HBD-3 concentrations correlate with the levels of the chemokines and the cytokines in the SIP and ASIP groups.

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EFFECTIVENESS OF AMLA JUICE IN REDUCING BLOOD GLUCOSE LEVEL AMONG PATIENTS WITH TYPE II DIABETES IN A SELECTED AREA OF KANYAKUMARI DISTRICT

THE RESEARCH RESERVOIR ◽

10.47211/trr.2021.v07i01.012 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Mrs. Vanitha. S s ◽

Dr. Pramjit kaur

Keyword(s):

Blood Glucose ◽

Standard Deviation ◽

Blood Glucose Level ◽

Glucose Level ◽

Type Ii Diabetes ◽

Control Group ◽

Type Ii ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Experimental Group

Challenges in lifestyle, such as increasesin energy intake and decreasesin physical activity are causing overweight and obesity leading to epidemic increases in type II Diabetes Mellitus. The research approach used for this study was evaluative approach and the research design was true experimental design. 60 patients with type II diabetes, 30 in experimental group and 30 in control group were selected for this study by using purposive sampling technique. Data was collected with the help of self-structured interview schedule. Descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (chi-square, paired ‘t’ test) were used to analyse the data and to test the hypotheses. In the experimental group,the pre-test mean score was 2.966, mean percentage was 59% and standard deviation was 1.129 and in post-testmean score was 2.533, mean percentage was 50.66% and standard deviation was 1.074 with effectiveness of 8.34% and paired‘t’ test value of t=3.971,which was statistically significant (p<0.05) which is an evidence ofthe effectiveness of Amla juice in reducing blood glucose level. Comparison of blood glucose levels in experimental and control groups, shows that the value is statistically highly significant, as was observed from the unpaired ‘t’ test value of 13.39 with P value of <0.05, which is an evidence indicatingthe effect of Amla juice in reducing postprandial blood glucose levels. The resultsfound that the administration of Amla juice did have aneffect in reducing blood glucose level in the experimental group. By comparing the findings of pre-test and post test between the experimental group and the control group,the effect was identified (assessed). The study concluded that the Amlajuice is effective in reducing blood glucose level.

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Pediococcus Acidilactici NRRL B-50517 as a Safe Probiotic for Regulation of Weight Related Pro-inflammatory Cytokines: A Double-blind Placebo-controlled Pilot Study (P21-026-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz041.p21-026-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Jhy Jhu Lin ◽

Jeanne O'Connell ◽

Steve Sinclair

Keyword(s):

Inflammatory Cytokines ◽

Body Fat ◽

Control Group ◽

Human Consumption ◽

P Value ◽

Double Blind ◽

Cytokine Analysis ◽

Survival Studies ◽

Mrs Broth ◽

Pro Inflammatory Cytokines

Abstract Objectives Development of a probiotic P. acidilactici NRRL B-50517 that resists to high temperature (up to 85°C), stomach acids, and air. Clinic evaluation of P. acidilactici NRRL B-50517 as a safe probiotic for regulation of weight related pro-inflammatory cytokines. Methods Survival studies were conducted by incubating P. acidilactici NRRL B-50517 in either liquid MRS broth, pH1.5 at 85°C overnight, or in dried powder at 85°C for 5–10 minutes. Healthy volunters were randomly assigned two capsules of placebo or Pediococcus probiotics (4 billion CFU) daily for three months. Biweekly phone interviews with 30 participants were performed to ensure daily dosage administration, and to collect the results of the questionnaire related to 6 categories of gut functions. Percent body fat measurements and serum were collected upon study initiation and completion. Serum was processed for cytokine analysis by multiplex ELISA assays. Results P. acidilactici NRRL B-50,517 survived at 85°C and pH 1.5 in MRS broth and dried powder forms. No statistical significantly changes of gut functions between the placebo group and the probiotic group in appetite: t-value 1.6808, p-value 0.1313; energy level: t-value 0.7508. p-value 0.5003; bowel movement: t-value 0.3348. p-value 0.7464; stool quality: t-value 0.6929, p-value 0.5080; bloating: t-value 2.0583. p-value 0.0736; gas t-value: 1.9560, p-value 0.0875. On average, the probiotic lost 0.86% ± 0.42% body fat whereas the control group gained 0.28% ± 0.19, P = 0.0264. Pro-inflammaroty IL-6 ratios differed by 0.61 ± 0.22 and 3.06 ± 0.87 in the probiotic and control groups, respectively (P = 0.0295); pro-inflammatory IL23 ratio was 0.65 ± 0.14 in the probiotic and 1.71 ± 0.38 in the control group, P = 0.0068. Conclusions Novel P. acidilactici NRRL B-50,517 is safe for human consumption and may be useful in reducing body fat and pro-inflammatory markers. Funding Sources TEDCO, Maryland.

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Lactobacillus helveticus and Bifidobacterium longum taken in combination reduce the apoptosis propensity in the limbic system after myocardial infarction in a rat model

British Journal Of Nutrition ◽

10.1017/s0007114509990766 ◽

2009 ◽

Vol 102 (10) ◽

pp. 1420-1425 ◽

Cited By ~ 34

Author(s):

Stéphanie-Anne Girard ◽

Thierno Madjou Bah ◽

Sévan Kaloustian ◽

Laura Lada-Moldovan ◽

Isabelle Rondeau ◽

...

Keyword(s):

Myocardial Infarction ◽

Inflammatory Cytokines ◽

Limbic System ◽

Bifidobacterium Longum ◽

Lactobacillus Helveticus ◽

Sprague Dawley ◽

Male Adult ◽

Gastrointestinal Problems ◽

Significant Difference ◽

Pro Inflammatory Cytokines

Myocardial infarction (MI) stimulates the release of pro-inflammatory substances that induce apoptosis in the limbic system. Pro-inflammatory cytokines are considered as the root cause of apoptosis, although the mechanism is not fully explained and/or understood at this time. In addition, depression may induce gastrointestinal perturbations that maintain the elevated levels of pro-inflammatory cytokines. It has been shown that some specific probiotic formulations may reduce gastrointestinal problems induced by stress and the pro/anti-inflammatory cytokine ratio. Therefore, we hypothesised that probiotics, when given prophylactically, may diminish the apoptosis propensity in the limbic system following a MI. Male adult Sprague–Dawley rats were given probiotics (Lactobacillus helveticus and Bifidobacterium longum in combination) or placebo in their drinking-water for four consecutive weeks. A MI was then induced in the rats by occluding the left anterior coronary artery for 40 min. Rats were killed following a 72 h reperfusion period. Infarct size was not different in the two groups. Bax/Bcl-2 (pro-apoptotic/anti-apoptotic) ratio and caspase-3 (pro-apoptotic) activity were reduced in the amygdala (lateral and medial), as well as in the dentate gyrus in the probiotics group when compared with the placebo. Akt activity (anti-apoptotic) was increased in these same three regions. No significant difference was observed in Ca1 and Ca3 for the different markers measured. In conclusion, the probiotics L. helveticus and B. longum, given in combination as preventive therapy, reduced the predisposition of apoptosis found in different cerebral regions following a MI.

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Impact of loganin on pro-inflammatory cytokines and depression- and anxiety-like behaviors in male diabetic rats

Physiology International ◽

10.1556/2060.105.2018.2.8 ◽

2018 ◽

Vol 105 (2) ◽

pp. 116-126 ◽

Cited By ~ 2

Author(s):

M Rajabi ◽

G Mohaddes ◽

F Farajdokht ◽

S Nayebi Rad ◽

M Mesgari ◽

...

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Inflammatory Cytokines ◽

Diabetic Rats ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Depression And Anxiety ◽

Tnf Α ◽

Immobility Time ◽

Pro Inflammatory Cytokines

Behavioral disturbances are observed in most patients suffering from diabetes. According to some evidence, pro-inflammatory cytokines have a key role both in diabetes and behavioral disorders, such as anxiety and depression. In this study, the effect of chronic administration of loganin, as a bioflavonoid, was investigated on pro-inflammatory cytokines and depression- and anxiety-like behaviors in streptozotocin-induced diabetes in male Wistar rats. Blood levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were assessed by enzyme-linked immunosorbent assay method. Depression- and anxiety-like behaviors were evaluated by forced swimming test (FST), elevated plus maze (EPM), and open field test (OFT), respectively. Body weight was also measured before the interventions and after the experiments in all groups. Our findings show that loganin-treated animals had significantly lower serum concentrations of IL-6 and TNF-α compared with the diabetic group. In the EPM test, loganin treatment significantly increased the percentage of the open arm time and open arm entries. Moreover, loganin treatment significantly decreased the grooming time and restored distance traveled and center crossing in the OFT. However, it decreased immobility time in the FST. Loganin treatment also significantly restored body weight gain and attenuated blood glucose changes in the diabetic rats. These results indicate that loganin possibly alleviates depression- and anxiety-like behaviors associated with diabetes through lowering the blood glucose and pro-inflammatory cytokine levels. More research is required to show the exact mechanism of antidepressant and anxiolytic effects of loganin in diabetes.

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ANTI-HYPERGLYCEMIC EFFECT OF DIFFERENT FRACTIONS OF ANNONA RETICULATA LEAF

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s2.13710 ◽

2016 ◽

pp. 256

Author(s):

Soumya Prakash Rout ◽

Durga Madhab Kar ◽

Laxmidhar Maharana

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Ethyl Acetate ◽

Glucose Level ◽

Ethyl Acetate Fraction ◽

Diabetic Control ◽

Control Group ◽

Alcoholic Extract ◽

Diabetic Control Group ◽

Annona Reticulata

ABSTRACT Context: Several species of the genus Annona were reported to have hypoglycemic properties and this makes Annona reticulata Linn. (Annonaceae) an interesting plant for investigating its anti-hyperglycemic potential. Objective: Different fractions prepared from hydro-alcoholic extract of A. reticulata leave were investigated for their blood glucose lowering effect on Streptozotocin (STZ) induced hyperglycemic rats. Methods: Ethyl acetate, methanol, and residual fractions (at dose level of 100 mg/kg by oral route) prepared from the hydro-alcoholic extract of A. reticulata leave were administered for 14 consecutive days to STZ induced hyperglycemic rats for evaluation of their anti-hyperglycemic potential. Anti-hyperglycemic potential was assessed by observation of a decrease in fasting blood glucose level. Results: The studies revealed that ethyl acetate fraction decreased the blood glucose level of hyperglycemic rats from 447.67 to 234.17 mg/dL and is significant (p<0.001) when compared with diabetic control group. The residual fraction and methanolic fraction decreased blood glucose level from 417.83 to 402.50 mg/dL and 432.33 to 371.67 mg/dL respectively but not significant when compared with the diabetic control group. Standard drug metformin (dose 300 mg/kg) reduced the blood glucose level from 447.33 to 219.50 mg/dL. Discussion: Ethyl acetate fraction at tested dose level was capable not only to control the elevated blood glucose level but also able to attenuate certain secondary parameters associated with STZ induced hyperglycemia. Conclusion: This study suggested that the ethyl acetate fraction prepared from hydro-alcoholic extract of A. reticulata leave exhibit potential antihyperglycemic property in the tested experimental models and should be investigated further. Keywords: Streptozotocin, Diabetes, Dyslipidemia.

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