scholarly journals Therapeutic and preventive effect of feed additives on the state of periodonts of rats with experimental dysbiosis

2021 ◽  
Vol 11 (11) ◽  
pp. 194-200
Author(s):  
A. Levitsky ◽  
A. Markov ◽  
T. Pupin ◽  
I. Selivanskaya ◽  
A. Lapinskaya
Keyword(s):  
Biochemical Parameters ◽  
The State ◽  
Feed Additives ◽  
Prophylactic Effect ◽  
Sunflower Seeds ◽  
Preventive Effect ◽  
High Oleic

Aim. Determine the therapeutic and prophylactic effect of feed additives on the condition of the periodontium in case of dysbiosis. Methods. As feed additives, we used flour from pea straw (FPS), oilcake from ordinary (high-linoleic) sunflower seeds (OOS) and oilcake from high-oleic sunflower seeds (OHOS), which were added to the composition of feed for rats in an amount of 10% instead of the same amount of grain wheat. The duration of feeding was 18 days. Experimental dysbiosis was reproduced using the antibiotic lincomycin. The activity of urease, lysozyme, catalase, elastase and the content of malondialdehyde (MDA) were determined in the gums of rats. Results. An increase in the level of urease, elastase, MDA and a slight decrease in the activity of lysozyme and catalase were found in rats with dysbiosis. The consumption of FPS and OOS had little effect on the biochemical parameters of the gums; however, the consumption of OHOS significantly reduced the activity of elastase, the level of MDA and normalized the level of urease. Conclusion. Under conditions of dysbiosis, periodontitis develops, which can be prevented by consuming high-oleic sunflower oilcake.

Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing system

2015 ◽  
Vol 18 (1) ◽  
pp. 131-139 ◽  
Author(s):  
F. Bubel ◽  
Z. Dobrzański ◽  
A. Gaweł ◽  
K. Pogoda-Sewerniak ◽  
E.R. Grela
Keyword(s):  
Alkaline Phosphatase ◽  
Biochemical Parameters ◽  
Blood Parameters ◽  
Feed Additives ◽  
Housing System ◽  
Serum Total Protein ◽  
Humic Materials ◽  
Limited Degree ◽  
Biochemical Indices ◽  
Food Mixture

AbstractAn influence of various humic-plant feed additives based on some herbs (nettle, chamomile, yarrow, perforatum), lucerne and humic materials on biochemical indices of Lohmann Brown (LB) layers blood plasma was estimated. Hens were housed in deep litter system, 20 birds in a group. Four groups were formed: control (C – standard feeding), and experimental, supplemented with preparations: E-1 herbal-humic, E-2 humic-herbal and E-3 – humic-lucerne. Hens were placed in the pens on the 16thweek of life, addition of preparations with standard food mixture started at the 22ndwk and lasted until 66thwk of life. Blood for analyses was collected four times in the following periods: 27, 37, 54 and 65thwk of life. The applied humic-plant preparations to a limited degree affected the values of examined biochemical parameters in serum: total protein (TP), albumins (Albs), glucose (Glu), urea, triacylglycerols (TAG), total cholesterol (TCh), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). It is difficult to determine based on these study, which preparation is one the most active biologically, however is seems that humic-lucerne preparation affected the examined blood parameters to the highest degree. The reference values ranges in hens blood serum LB hens were proposed for: TP (43-65 g/l), Albs (15-22 g/l), urea (0.5-1.2 mmol/l), Glu 10-15 mmol/l), TCh (2.2-4.5 mmol/l), TAG (10-24 mmol/l), AST (4-12 U/l), ALT (150-280 mmol/l) and ALP (190-350 U/l).

Prophylactic Effect of Dragon-Pearl Tea on HCl/Ethanol Induced Gastric Injury in ICR Mice

2014 ◽  
Vol 651-653 ◽  
pp. 273-276
Author(s):  
Rui Wang ◽  
Xin Zhao
Keyword(s):  
Gastric Secretion ◽  
Inhibitory Effect ◽  
Gastric Injury ◽  
Prophylactic Effect ◽  
Preventive Effect ◽  
Icr Mice ◽  
Low Concentrations ◽  
Tnf Α ◽  
High Concentrations ◽  
Preventive Effects

Dragon-pearl tea is a traditional Chinese drink consumed in Southwest of China. In the present study, the preventive effect of Dragon-pearl tea on gastric injury was determined in ICR mice. High concentrations of Dragon-pearl tea were observed to reduce the levels of the serum proinflammatory cytokines, IL-6 and TNF-α, compared with low concentrations of Dragon-pearl tea. Gastric secretion volumes were highest in control mice and reduced in 500, 1,000 and 1,500 mg/kg Dragon-pearl tea treated mice. The pH levels of gastric juice samples obtained from each group revealed the opposite correlation. Gastric injury levels in mice treated with Dragon-pearl tea were identified to be significantly reduced, demonstrating its anti-inflammatory properties. Administration of 1,500 mg Dragon-pearl tea (79.4%) by gavage was demonstrated to induce the highest inhibitory effect on gastric injury. Results of the current study indicate that Dragon-pearl tea exhibits marked preventive effects on gastric injury.

scholarly journals Dynamics of connective tissue metabolites in the blood of rats of different age under hypokinesia

2021 ◽  
pp. 42-45
Author(s):  
D. Morozenko ◽  
F. Leontieva ◽  
K. Gliebova
Keyword(s):  
Connective Tissue ◽  
Motor Activity ◽  
Blood Serum ◽  
Biochemical Parameters ◽  
Fractional Composition ◽  
Sialic Acids ◽  
The State ◽  
The Body ◽  
Male Rats ◽  
Chondroitin Sulfates

Topicality. Hypokinesia is a condition of insufficient motor activity of the body with limited pace and volume of movement, and is currently the fourth leading cause of endemic death in the world. It is known that hypokinesia can affect the metabolism of components of bone and cartilage, which is the basis for the development of pathological processes, but there are no biochemical markers of this process in the literature. Thus, currently, there is insufficient number of experimental scientific works devoted to the study of biochemical parameters characterizing the state of the connective tissue in the experiment to assess the impact of hypokinesia on the body, and this fact determines the relevance of the study. Aim. To study the dynamics of the main metabolites characterizing the state of the connective tissue in the blood serum in hypokinesia in rats of different ages. Materials and methods. The studies were performed on 42 white male rats aged 3 and 12 months, kept in the vivarium of the Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv. The conditions of hypokinesia were reproduced using a specially designed device that limited the mobility of the animal without disturbing the ventilation of the body. Animals were removed from the experiment by decapitation in Day 7 and 30 of immobilization under thiopental anesthesia. The content of sialic acids in the serum was determined by the Hess method, chondroitin sulfate – by Nemeth-Csoka method modified by L. I. Slutsky, the fractional composition of glycosaminoglycans (GAG) – by the reaction with resoquine. Results and discussion. In the study of the rat serum, it was found that in intact animals of 3 and 12 months of age the content of sialic acids was different; it was 2.94 ± 0.16 and 1.98 ± 0.09 mmol/L, respectively. After 7 days of hypokinesia, their concentration in young animals did not change, but in older animals, this figure increased. By Day 30, the serum sialic acid levels suddenly increased compared to intact animals. In animals of 12 months of age, the concentration of sialic acids in the blood serum increased by both Day 7 and Day 30 of hypokinesia, especially at the end of the experiment. Hypokinesia also showed a significant increase in serum concentrations of chondroitin sulfates on Day 7 of the experiment in animals of both age groups. At the same time, the level of these metabolites on Day 30 was at the same level. In young rats on Day 7 the fraction of GAG remained unchanged, but on Day 30 there was a significant decrease. During this period, the second fraction of GAG increased. In animals aged 12 months on Day 7 and 30, the content of most GAG fractions was lower than the values characterizing the fractional composition of GAG in intact rats. Conclusions. Thus, the determination of sialic acids, GAG fractions and chondroitin sulfates in the serum can be presented as a set of biochemical tests to assess disorders of the connective tissue metabolism while limiting the motor activity of the body. It has been found that the 30-day hypokinesia causes significant disorders of the connective tissue, and it is reflected in changes in the biochemical parameters of the serum of experimental rats.

scholarly journals Prediction of birth and postpartum pathology in deep-calving heifers by biochemical parameters of blood

2021 ◽  
Vol 23 (101) ◽  
pp. 21-25
Author(s):  
L. V. Koreyba
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Mineral Metabolism ◽  
Obstetric Care ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The State ◽  
Total Protein Content ◽  
Carotene Content

The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.

scholarly journals Comparison of the effects of basil (<i>Ocimum basilicum</i>) essential oil, avilamycin and protexin on broiler performance, blood biochemistry and carcass characteristics

2015 ◽  
Vol 58 (2) ◽  
pp. 425-432 ◽  
Author(s):  
S. R. Riyazi ◽  
Y. Ebrahimnezhad ◽  
S. A. Hosseini ◽  
A. Meimandipour ◽  
A. Ghorbani
Keyword(s):  
Essential Oil ◽  
Biochemical Parameters ◽  
Body Weight Gain ◽  
Density Lipoprotein ◽  
Serum Triglyceride ◽  
Carcass Characteristics ◽  
Feed Additives ◽  
Control Group ◽  
Growth Promoter ◽  
Feed Conversion

Abstract. The effects of a probiotic (protexin), a medicinal plant (basil essential oil) and an antibiotic growth promoter (avilamycin) as broiler feed additives on performance, carcass characteristics and some blood parameters of broilers were studied. A total of 600 Arian broilers were divided into six treatments, with four replicates of 25 birds. Treatments were a plant essential oil in three levels (200, 400 and 600 ppm), the probiotic (150 ppm), the antibiotic (150 ppm) and a control group with no additives. Birds in different treatments received the same diets during the experimental period. Growth performance, blood biochemical parameters and carcass traits were measured. There were no effects of dietary treatment on body weight gain, feed intake and feed conversion ratio of the broilers. Internal organ weights and carcass characteristics were not influenced by treatments; however, 200 ppm basil essential oil supplementation decreased abdominal fat (P < 0.05). Biochemical parameters including serum low-density lipoprotein (LDL), high-density lipoprotein (HDL) and total cholesterol were not statistically influenced. However, addition of 400 ppm basil essential oil into diet increased serum triglyceride as compared to the control (P < 0.05). Results of this experiment demonstrated that the herbal natural feed additives such as basil may be used as alternatives to an antibiotic growth promoter without any adverse effects on broiler production.

Symbiotic effect of lactobacillus acidophilus, Ginger, Pineapple and Green Coffee in the complex management of obesity in rats

2021 ◽  
Vol 30 (1) ◽  
pp. 143-152
Author(s):  
Aml Ghanem ◽  
Osama M. Abonama ◽  
Ahmed I. Abd El Maksoud ◽  
Mokhtar M. El-Zawahry ◽  
Dalia Elebeedy
Keyword(s):  
Body Fat ◽  
Lactobacillus Acidophilus ◽  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Positive Control ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Green Coffee ◽  
Sprague Dawley

Background: Obesity is a chronic metabolic disease associated with having excess body fat that could be influenced by many factors. Our study aimed to assess the powerful effect of Lactobacillus acidophilus alone or combined (symbiotic) with Prebiotic such as Ginger, Pineapple and Green Coffee as anti-obesity agents. Methodology: Using 8 groups (10 rats each) of Sprague-Dawley rats, Group 1 was kept as a negative control, Group 2 positive control, while other groups were orally given Lactobacillus acidophilus, Ginger, Pineapple and Green Coffee individually and in combination with Probiotics, for 45 days till the end of the experiment while the body weight of rats was recorded.Blood samples were collected for biochemical parameters analysis and organs were dissected and homogenized to analyze obesity-related biomarkers, Results: Our results revealed that either individual or mixed administration of this pro and prebiotics decreased the body and organs, specifically those treated with the mixture or probiotic and prebiotic, also serum (HDL), CAT), and (SOD) was decreased (P <0.05), while other biochemical parameters (T.G), (CHOL), (U.A), (Creat), Urea, (GOT),(GPT) and (ALP); ( significantly (P<0.05) was decreased when compared with the positive control group, Nevertheless, the histopathological examination showed the reduction of adipose tissue in kidney, liver, and Pancreas showed overestimate reductions in the percentage of body fat. Conclusions: This study showed a promising effect of Lactobacillus acidophilus when it combined with these plants as natural feed additives on obesity.

The use of probiotics for the correction of disturbances of metabolism in cows

2021 ◽  
pp. 47-53
Author(s):  
O. V. Kovaleva ◽  
N. V. Sannikova ◽  
О. V. Shulepova ◽  
N. M. Kostomakhin ◽  
Yu. A. Karmatskikh
Keyword(s):  
Bacillus Subtilis ◽  
Milk Yield ◽  
Biochemical Parameters ◽  
Metabolic Disorders ◽  
Ketone Bodies ◽  
Animal Husbandry ◽  
Feed Additives ◽  
Coliform Bacteria ◽  
Lactating Cows ◽  
Microbial Number

Currently, there is a huge range of different feed additives on the market, which are widely used in animal husbandry, but they act simultaneously, i. e. by stopping their use the resulting effect is also lost. Metabolic disorders due to feeding errors begin imperceptibly without any precursors, and first of all this affects the biochemical parameters of the blood only later leads to alimentary diseases with deep often irreversible degenerative changes in organs and tissues. In practice, there are rarely disorders of any one type of metabolism more often there are combinations of various metabolic disorders, especially often this occurs in the absence of a complete balanced feeding. The purpose of the work was to evaluate the effectiveness of the use of a probiotic drug based on Bacillus subtilis in the watering of lactating cows for the correction of metabolic disorders. In order to assess the actual presence of probiotics in the water during the experiment its analysis has been carried out. It has shown that in the water that enters the distribution tank the total microbial number was 9×10[sup]5[/sup] CFU/ml, and in the drinker this indicator was at the level of 3,8×10[sup]5[/sup] CFU/ml. Common coliform bacteria and thermotolerant coliform bacteria have been not detected. Studies have shown that when using the drug based on Bacillus subtilis containing five strains of bacteria in an amount of 0,01 %, which is 6 ml/head/day in the watering of lactating cows during the increasing the milk yield period there was a tendency to correct metabolic disorders. There was the decrease in the content of ketone bodies by 11,9 %, the increase in glucose by 34 % and the decrease in urea by 7,1 mmol/l.

Die Präventionswirkung des Staatshaftungsrechts

2021 ◽  
Vol 54 (2) ◽  
pp. 189-222
Author(s):  
Torben Ellerbrok
Keyword(s):  
The State ◽  
Public Official ◽  
Economic Analysis Of Law ◽  
Preventive Effect ◽  
German State ◽  
State Sector ◽  
Liability Law ◽  
The One ◽  
State Liability ◽  
Liability Claims

This contribution addresses the question of whether and to what extent state liability law has a preventive effect. The question focuses on whether the (mere) existence of claims under state liability law creates an incentive to prevent unlawful actions against citizens. Cases in which an incentive is created for an individual public official (individual preventive effect) are differentiated from those in which an incentive is created for the state as an organisation (organisational preventive effect). Based on an economic analysis of law, the article demonstrates – in abstract terms and detached from concrete bases for claims – that both effects can occur: On the one hand, liability claims can prevent public officials from deliberately making unlawful decisions and as well as increase the diligence and so the probability of a lawful decision. On the other hand, liability claims can influence an organisation in the state sector in such a way that those responsible strive to ensure lawful decisions by means of the improved structuring and scrutiny of the decision-making process. To establish the specific preventive effect of an individual claim under (German) state liability law, it is necessary to consider its specific structure. The allocation of liability and the possibility of an internal recourse determine whether the preventive effect achieved is (more) an individual or an organisational one. The decisive criteria for the intensity of the preventive effect include the type, extent, degree of collectivisation, and limitations of the liability claim as well as its enforceability. Regardless of the necessary differentiation, it may be concluded that the claims of German state liability law emphasise an organisational preventive effect. While they implement the preventive effect to some extent overall, they do not fully do so. There are viable reasons for this restraint, considering the at times contrary objective of an efficiently acting administration. As a result, as this contribution concludes, from a legal policy perspective, a preventive effect also depends on considering additions and alternatives to state liability law.

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