scholarly journals Effects of salinity and external proline on seed germination and early growth of Cucumis sativus cv. super ps

2018 ◽  
Vol 10 (4) ◽  
pp. 215-220
Author(s):  
FARIBA AMINI ◽  
MEHRY ASKARY ◽  
FAHIMEH MOHTASHAMI ◽  
FAHIMEH MOHTASHAMI

Amini F, Askary M, Mohtashami F. 2018. Effects of salinity and external proline on seed germination and early growth of Cucumis sativus cv. super ps. Nusantara Bioscience 10: 215-220. Salinity is one of the important abiotic stresses that has an adverse effect on plant productions. High salt concentrations had negative effects on plant processes such as seed germination, seedling growth and turgescent, vegetative growth, flowering and fruiting stages. Proline as a compatible solution plays an important role in defense mechanisms of cellular stress. This study was done to evaluate effects of proline pretreatment (0 and 20 mM) in terms of salinity (0, 50, 100 and 150 mM) on cucumber cv. super ps. The obtained results proved that salinity treatments had significant effects on seed germination rate. On the contrary, application of proline had no significant effect on germination percentages of control and salt-stressed seeds. The root fresh weight and length of plant reduced with salinity increment while proline treatment had positive significant effects on the root dry as well as the fresh weight. Although, no significant variation was found in the root length and dry weight of cultivars treated with proline under salinity stresses. Meanwhile, the shoot length and dry weight of plants declined after salt-stressed treatments. While this condition increased the fresh weight of shoot. Proline treatments increased all of observed parameters but there were no significant variation found in the root length or dry weight of plants treated under saline stresses. The total soluble carbohydrate of plants had positive significant correlation with salt concentration, but the application of proline declined carbohydrate amounts in both salt-stressed and non-stressed plant. Salinity stress increased sodium, proline, protein contents and CAT enzyme, GPOX as well as MDA concentrations, while in the proline-treated plants salinity stresses had no significant effect on CAT, GPOX, MDA concentrations and internal proline amount.

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.


2020 ◽  
Vol 1 (6) ◽  
pp. 1-5
Author(s):  
Filza Ghafoor ◽  
Sana Liaqat ◽  
Waseem Iqbal

Seed priming is a pre-sowing treatment used to reduce stress effects in crops .This technique has been used to increase the germination rate of seeds mainly under unfavorable environmental condition. Crop productivity faces many problems like that unavailability of suitable condition that causes unbalanced seedling growth and poor germination. Hormones are essential to improve plant growth, development, crop yield quality and quantity. Auxin is essential hormones that improves germination of seeds and reduce stress effects. Laboratory experiment was conducted to study the effect of seed priming using different concentrations of IAA and IBA on carrot seeds (Daucus carota). In laboratory experiment, three concentrations of IAA and IBA (75 ppm, 50ppm, 25ppm) treated with distilled water with priming duration of 24 hours. Experiment was laid out in CRD with three replications. Data was collected to investigate the effect of seed priming with IAA and IBA under these condition like that (fresh weight, dry weight, speed of germination, root length, seed length, seedling length, final germination rate, mean germination time and dry matter content). Maximum fresh weight, final germination percentage, seedling length, energy of germination, speed of germination and mean germination rate was recorded IBA 75ppm. Maximum dry weight and dry matter content was observed IBA 25ppm.Maximum shoot length was recorded IBA 50ppm. Non-significant difference was recorded in root length.


2021 ◽  
Vol 63 (3) ◽  
pp. 42-47
Author(s):  
Quy Tung Le ◽  
◽  
Chiti Sritontip ◽  

Nano-bubbles (NBs) demonstrate their full potential in fields like agriculture, aquaculture, food processing, and wastewater treating. This study investigated the effects of air NBs on the progress of seed germination of muskmelon. Distilled water was treated with NBs under time schedules of 10, 15, and 20 min and untreated distilled water was used as the control. Statistically significant differences in seed germination rate occurred between treatments. Growth indicators such as stem height, root length, and leaf area were measured after 14 d after treatment with water containing NBs. Statistical analysis showed that there were significant differences in the dry weight of samples between treatments although these differences were quite small.


2022 ◽  
Vol 951 (1) ◽  
pp. 012065
Author(s):  
K A Tanjung ◽  
L A M Siregar ◽  
R I M Damanik

Abstract This study aims to determine the effect of the application of plant growth regulators and osmoconditioning treatment to improve the germination of true shallot seeds. This research was conducted in Asam Kumbang, Medan Selayang, Medan, Indonesia. The research method was a Randomize Block Design with 2 factors, the first factor is Plant Growth Regulators (Z) with 6 levels, namely Z0 (Without PGRs Application), Z1 (Gibberellin 500 ppm), Z2 (Putrescine 15 ppm), Z3 (Putrescine 20 ppm), Z4 (Putrescine 15 ppm + Gibberellin 500 ppm), Z5 (Putrescine 20 ppm + Gibberellin 500 ppm). The second factor was the osmoconditioning treatment with Polyethylene Glycol (PEG) 6000 (O) with 4 levels, namely O0 (Without Osmoconditioning Treatment), O1 (PEG 6000 3%), O2 (PEG 6000 4%), O3 (PEG 6000 5%). Parameters observed were germination rate, germination rate index, percentage of germination, germination ability, simultaneous growth of seeds, seedling length, root length, seedling dry weight, and catalase activity test. The results of this study were: application of plant growth regulators could improve true shallot seed germination, indicated by the observed values of all parameters which were significantly different from those of the control (without PGRs application). The plant growth regulator that produced the best increase in germination was Gibberellins 500 ppm, although the difference in effect with other PGRs was not significantly different. Meanwhile, the osmoconditioning treatment with PEG 6000 was also able to improve the germination of true shallot seeds as indicated by an increase in most of the observed parameters, but in the root length parameter it was seen that the tendency of PEG 6000 3% always gave the highest value but gave the lowest value for this parameter. The best concentration of PEG 6000 in the osmoconditioning treatment to improve true shallot seed germination was 3%.


Author(s):  
Ferit Özen ◽  
Gülsüm Yaldız ◽  
Mahmut Çamlıca ◽  
Halit Aşkın ◽  
Abdurrahman Başol

This research was carried out in the climate chamber in order to determine the effect of hydrosols obtained from sage grown with selenium application on germination of fenugreek and coriander seeds. Different concentrations of sage hydrosol (2.5, 5, 7.5 and 10 ml) and purified water used as control were applied to the fenugreek and coriander seeds. Germination rate, fresh weight, dry weight, root length, shoot length and root / shoot ratio of fenugreek and coriander seeds were applied. Variance analysis was performed according to the split plot design and the mean values were compared with LSD test. According to the results of the research; germination rate of fenugreek seeds 38.35-51.00%, fresh weight 0.04-0.12 g, dry weight 0.001-0.035 g, root length 0.71-2.02 cm, shoot length 1.27-3.20 cm, root/shoot ratio varied between 0.34-0.82, germination rate of coriander seeds 18.61-39.21%, fresh weight 0.0011-0.034 g, dry weight 0.004-0.030 g, root length 1.5-4.58 cm, shoot length 1.08-3.69 cm, root/shoot ratio 0.47-2.51 were found. The highest germination rate was obtained in 2.5 ml dose of sage hydrosol (51.00%) in fenugreek seeds and the highest germination rate was found in 7.5 ml dose of sage hydrosol (39.21%) in coriander seeds.


2015 ◽  
Vol 43 (1) ◽  
pp. 108-112 ◽  
Author(s):  
Mehmet YILDIRIM ◽  
Ferhat KIZILGECI ◽  
Cuma AKINCI ◽  
Onder ALBAYRAK

Salinity is an important source of abiotic stress, limiting crop performance in most arid and semi-arid areas of the world. This research was conducted to determine the effects of salinity on physiological parameters of durum wheat (Triticum durum Desf.) genotypes. The research was conducted in the tissue culture laboratory at the Agriculture Faculty of Dicle University. The study consisted of one durum wheat commercial cultivar, five local cultivars and four advanced genotypes. There were three replications in a split-plot experimental design. Genotypes were germinated in four NaCl concentrations (0, 50, 100, 150 mM) in plastic boxes. There were statistically assured significant differences among the genotypes for all salt concentrations and all observed parameters (coleoptile length, seedling length, root length, seedling fresh weight, root fresh weight, seedling dry weight, root dry weight, germination rate and seedling vigor). There was significant decrease in all examined parameters depending on the increase of salt concentration. The ‘Sorgul’ genotype was most tolerant to salinity, in terms of root length and root dry weight, whereas ‘Altintoprak 98’ was most tolerant as measured by the impact of salinity on coleoptile length, seedling fresh weight, germination rate and seedling vigour. The ‘Beyaziye’ genotype was the most sensitive to salinity-induced stress. The results from this study demonstrated differences among durum wheat genotypes for seedling parameters measured in the presence of salinity stress.


2020 ◽  
Vol 19 (2) ◽  
pp. 168-174
Author(s):  
Antônio Jussiê da Silva Solino ◽  
Juliana Santos Batista de Oliveira ◽  
Maycon Schmitt ◽  
Jéssica Brasau da Silva ◽  
Kátia Regina Freitas Schwan-Estrada

Biostimulating microorganisms have protector effect against pathogenic agents, affect the stand formation, promote plant growth, and increase yield of agricultural crops. Thus, the objective of this work was to evaluate the seed germination and seedling emergence of different common bean cultivars as a function of treatments with Bacillus subtilis BV02 and Trichoderma asperellum BV10. Germination tests in paper rolls and sand were conducted in a completely randomized design, with a 2×4 factorial arrangement consisted of two cultivars, namely Campos Gerais (CG) and Estilo (ES), and four microorganism treatments, namely B. subtilis BV02 (BS), T. asperellum BV10 (TA), B. subtilis BV02 + T. asperellum BV10 (BS+TA), and Control. The variables analyzed were: germination speed index (GSI), emergence speed index (ESI), germination percentage, emergence percentage, root length, seedling height, and root and shoot fresh and dry weights. The CG cultivar had higher GSI, ESI, root length, seedling height, shoot fresh weight, root fresh weight, and shoot dry weight than the ES cultivar in the paper roll and sand tests. The common bean seed inoculation with BS+TA increased seedling height, shoot fresh weight, shoot dry weight, and root length in the ES cultivar. The CG cultivar had higher GSI, ESI, root length, root fresh weight, and root and shoot dry weights than the ES cultivar. The treatment of seeds with BS+TA is recommended to improve the performance of common bean seedlings of the ES cultivar.


2021 ◽  
Vol 24 (6) ◽  
pp. 609-617
Author(s):  
Seo Youn Lee ◽  
Seo Hee Jung ◽  
Ah Ram Cho ◽  
Myung Syun Shim ◽  
You Kyung Chung ◽  
...  

Background and objective: The nanobubbles remain stable in water, and it increased dissolved oxygen (DO) in the water that promotes the seed germination and the plant growth. We evaluated the seed germination and growth of sprouts (radish, wheat, and barley) and leafy vegetables (red mustard and pak choi) when irrigated with various DO of nanobubble water (NB).Methods: The oxygen NB was generated by surface friction and treated in 4 levels: NB 0% (control, DO 9.21 mg·L-1), NB 20% (DO 15.40 mg·L-1), NB 33% (DO 20.93 mg·L-1), and NB 100% (DO 39.29 mg·L-1).Results: The root length of radish and wheat increased more in NB 33% than the control plot. The fresh weight increased in NB 33% compared to the control plot in radish and wheat, and both fresh and dry weight increased more in NB 20%, NB 33%, and NB 100% than the control plot of barley. The leaf length and width of red mustard decreased more in NB 33% and NB 100% than the control plot and NB 20%, which indicated the leaf compactness. The fresh and dry weight of shoot and root increased more in NB 100% than the control plot in red mustard. In pak choi, the shoot fresh weight increased more in NB 100% than the control plot. In leafy vegetables, the germination rate of red mustard in NB 100% was higher than the control plot, however, it was not significantly different between oxygen NB plots in sprout vegetables.Conclusion: The results showed that the root growth and biomass increased after applying NB 33% in sprout vegetables. The leaf growth properties as the number of leaves and leaf size were not significantly different or decreased in NB treatments compared to control plots, but NB 100% (DO 39.29 mg·L-1) effectively increased the root growth and plant biomass in leafy vegetables.


Atmosphere ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Siaka Dembélé ◽  
Robert B. Zougmoré ◽  
Adama Coulibaly ◽  
John P. A. Lamers ◽  
Jonathan P. Tetteh

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.


2021 ◽  
Vol 43 ◽  
Author(s):  
Nancy Araceli Godínez-Garrido ◽  
Juan Gabriel Ramírez-Pimentel ◽  
Jorge Covarrubias-Prieto ◽  
Francisco Cervantes-Ortiz ◽  
Artemio Pérez-López ◽  
...  

Abstract: Chitosan is a biopolymer obtained from deacetylation of chitin; it has multiple applications in agriculture as an antifungal, soil conditioner, inducer of defense mechanisms, fruits postharvest coating, leaves and seeds, among others. The objective in this research was to evaluate the effect of chitosan coatings mixed with fungicide (dithiocarbamate) on the germination and germination speed of bean and maize seeds in storage and to determine the retention capacity of the fungicide in the coated seeds under different times of imbibition. Two coating treatments at concentrations of 0.1 and 0.5% chitosan in water, two coatings treatments at 0.1 and 0.5% chitosan supplemented with 0.5% fungicide and a coating without chitosan using only 0.5% fungicide in water were used in bean and maize seed; and as control seeds imbibed in distilled water were used; after treatments, germination percentage and germination speed were determined, also fungicide release were determined at 0, 1, 2 and 6 h of imbibition, and the effect of storage time on germination and germination speed was determined at 30, 60, 90, 120, 150 and 180 days of storage at 4 °C and 45% relative humidity. The fungicide release effect was determined by inhibiting Fusarium oxysporum conidia germination. There were no negative effects of coatings on seed germination after storage. The treatment that provided both greater retention of the fungicidal agent and released it gradually, was 0.5% chitosan mixed with fungicide concentration. Chitosan coating seeds mixed with fungicide do not cause negative changes in seed germination or germination rate.


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