ANTIRADICAL ACTIVITY OF EXTRACTS FROM SIBERIAN FLORA GENUS CENTAUREA PLANTS

The purpose of this research is to study the antiradical activity of various extracts from the Centaurea scabiosa L. aerial part (wild-growing and cultivated) and Centaurea jacea L. as potential sources of antioxidant herbal remedies. In the study of the antiradical activity of extracts from the aerial part of Centaurea jacea L., Centaurea scabiosa L. wild growing and cultivated, in the reaction with a stable diphenylpicrylhydrazyl radical, it was found that the degree of antiradical activity within each species correlates with the quantitative content of flavonoids. Lower values of the antiradical activity of cultivated Centaurea scabiosa L. extracts compared to wild growing Centaurea scabiosa L. extracts are consistent with a lower content of flavonoids in the latter. The dependence of the Centaurea jacea L. and wild growing Centaurea scabiosa L. extracts antiradical activity on flavonoids quantitative and qualitative composition of these species has been established.

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Content of amino acids in raw materials of somespecies of ASTERACEAE family

Fitoterapia ◽

10.33617/2522-9680-2020-3-44 ◽

2020 ◽

Vol 3 (3) ◽

pp. 44-50

Author(s):

S.M. Marchyshyn ◽

◽

L.V. Slobodianiuk ◽

R.Yu. Basaraba ◽

N.А. Hudz ◽

...

Keyword(s):

Amino Acids ◽

High Performance Liquid Chromatography ◽

Free Amino ◽

High Performance ◽

Raw Materials ◽

Essential Amino Acids ◽

Qualitative Composition ◽

Quantitative Content ◽

First Time ◽

Asteraceae Family

Keywords: amino acids; common pussytoes; yacon; stevia; golden marigolds; leaves; herb; aster family; high performance liquid chromatography. For the first time the qualitative composition was studied and the quantitative content of amino acids in the herb of common pussytoes, yacon leaves, stevia leaves, herb of golden marigolds was determined by HPLC. 17 bound and 16 free amino acids were identified in the herb of common pussytoes, in the herb of golden marigolds, yacon leaves and stevia leaves – 12 and 5, 17 and 11 and 16 and 14 amino acids, respectively. The highest content of the sum of essential and substitute amino acids is contained in the leaves of stevia; the lowest content of the sum of essential amino – in the herb of golden marigolds, the sum of essential amino acids – in the herb of common pussytoes.

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Determination of hydroxycinnamic acids in the herb of stachys sieboldii miq

Medical and Clinical Chemistry ◽

10.11603/mcch.2410-681x.2016.v0.i3.6935 ◽

2016 ◽

Author(s):

S. M. Marchyshyn ◽

L. V. Husak ◽

T. S. Berdey

Keyword(s):

Hydroxycinnamic Acids ◽

Qualitative Composition ◽

Quantitative Content ◽

Stachys Sieboldii

In the article the results of study of qualitative composition and quantitative content of hydroxycinnamic acids in the herb of Stachys sieboldii Miq. are presented. By the method of HPLC it was established the presence of chlorogenic, rosemarinic, caffeic and p-coumaric acids. Quantitative content of hydroxycinnamic acids was determined by the method of spectrophotometry.

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Fatty acid composition of vegetable marrows and zucchini leaves

Pharmacia ◽

10.3897/pharmacia.66.e37893 ◽

2019 ◽

Vol 66 (4) ◽

pp. 201-207 ◽

Cited By ~ 2

Author(s):

Olena O. Iosypenko ◽

Viktoriia S. Kyslychenko ◽

Zinaida I. Omelchenko ◽

Iryna S. Burlaka

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Plant Material ◽

Cucurbita Pepo ◽

Physiological Condition ◽

Unsaturated Fatty Acids ◽

Total Content ◽

Qualitative Composition ◽

Quantitative Content

The qualitative composition and quantitative content of fatty acids in leaves of vegetable marrows (Cucurbita pepo L. var. giromontina Alef.), zucchini (Cucurbita pepo L. var. cylindrica Paris) yellow- and green-fruits varieties were determined by using GC/MS. 14 fatty acids were identified as the result of the experiment. Unsaturated fatty acids were found to be dominated in the raw plant material. The total content of unsaturated fatty acids amounted to 60.47% in vegetable marrows leaves, 64.35% yellow zucchini leaves and 68.85% green zucchini leaves, among which linoleic and linolenic acid dominated. The related health lipid indices (IA, atherogenicity, IT, thrombogenicity and IH, health) were determined. It is shown that the use of such biological resources is actual and expedient for the purpose of alimentary correction of the physiological condition of a person.

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The study of polyphenolic compounds of Pimpinella anisum herb and determination of their antioxidant activity

Žurnal organìčnoï ta farmacevtičnoï hìmìï ◽

10.24959/ophcj.21.226276 ◽

2021 ◽

Vol 19 (1(73)) ◽

pp. 42-47

Author(s):

U. Umarov ◽

S. V. Kolisnyk ◽

O. V. Kolisnyk ◽

M. Fatkhullaeva ◽

N. K. Chinibekova ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Ethanolic Extract ◽

Polyphenolic Compounds ◽

Qualitative Composition ◽

Pimpinella Anisum ◽

Quantitative Content

Aim. To study the qualitative composition and quantitative content of the polyphenolic compounds in Pimpinella anisum herb and determine their antioxidant activity.Results and discussion. Among the polyphenolic compounds contained in anise herb, chlorogenic acid(4.409 mg/g) predominates. Signifcant amounts of catechins (3.104 mg/g), apigenin derivatives (3.077 mg/g) and luteolin (1.864 mg/g) also accumulate in the herb. Minor amounts of myricetin (0.105 mg/g) and naringenin (0.019 mg/g) derivatives, rutin (0.189 mg/g), quercetin (0.028 mg/g), apigenin (0.009 mg/g) and hesperetin (0.002 mg/g) are present. According to the research results, the antioxidant activity of polyphenolic compounds of anise herb with reference to ascorbic acid was found to be 67.76 ± 0.05 mmol/g. Rutin exhibited the antioxidant activity at the level of 3979.59 ± 0.08 mmol/g.Experimental part. Pimpinella anisum herb collected during the ﬂowering stage in the summer of 2019 inthe Kharkiv region (Ukraine) was used for analysis. The analysis of 70 % ethanolic extract from anise herb was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversedphase, C18 modifed silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Identifcation of substances in the extract was carried out by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. The antioxidant activity was determined by the potentiometric method (pH meter – Hanna 2550, with redox electrode EZDO PO50) with reference to ascorbic acid.Conclusions. The qualitative composition and quantitative content of polyphenolic compounds in the ethanolic extract of anise herb have been determined by high performance liquid chromatography. The total content of polyphenolic compounds is 17.576 mg/g. The antioxidant activity of polyphenolic compounds of anise herb with reference to ascorbic acid has been found to be 67.76 ± 0.05 mmol/g.Key words: polyphenolic compounds; anise; herb; high performance liquid chromatography; antioxidant activity

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Analysis of carbohydrates in Saponaria officinalis L. using GC/MS method

Pharmacia ◽

10.3897/pharmacia.68.e62691 ◽

2021 ◽

Vol 68 (2) ◽

pp. 339-345

Author(s):

Liudmyla Slobodianiuk ◽

Liliia Budniak ◽

Svitlana Marchyshyn ◽

Liliya Kostyshyn ◽

Oleksandr Zakharchuk

Keyword(s):

Fatty Acids ◽

North Africa ◽

Qualitative Composition ◽

Quantitative Content ◽

Lack Of Information ◽

The World ◽

The Common ◽

Free Carbohydrates ◽

Saponaria Officinalis ◽

Quillaic Acid

Saponaria officinalis L. (common soapwort), usually named fuller’s herb, is encountered in most of Europe, in Spain, France, Italy, for example, and also in Syria and North Africa. Saponaria officinalis L. is known in most of the world as an introduced species, often a weed, and sometimes as cultivated decorative plant. Saponaria officinalis contains a large amount of saponins, which foam during extraction with water. In addition to saponins, common soapwort also contains flavonoids, quillaic acid, fatty acids and different phenolic compounds. There is a lack of information about carbohydrates content of common soapwort. Thus the aim of this study was to determine the content of carbohydrates Saponaria officinalis L. herb and roots. The qualitative composition and quantitative content of carbohydrates in herb and roots of Saponaria officinalis L. were determined by using GC/MS method. The studies have shown that Saponaria officinalis L. herb is mainly composed of free carbohydrates such as D-glucose (3.65 mg/g), D-galactose (0.29 mg/g), D-fructose (0.20 mg/g) and D-saccharose (3.72 mg/g). In common soapwort herb, after acidic hydrolysis and derivatization with acetylated aldononitriles, D-arabinose, D-fucose, D-mannose, D-glucose, D-galactose, D-fructose and Myo-inositol were identified. Free carbohydrates in the roots of Saponaria officinalis L., including D-glucose, D-galactose and D-saccharose, were determined with GC/MS method too. D-saccharose was common among free carbohydrates of Saponaria officinalis L. in the largest amounts. Its content in herb and roots of the common soapwort was 3.72 mg/g and 25.39 mg/g respectively.

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Bioactive compounds and antiradical activity of propolis from scaptotrigona bipunctata e scaptotrigona depilis / Compostos bioativos e atividade antiradical da própolis de scaptotrigona bipunctata e scaptotrigona depilis

Brazilian Journal of Health Review ◽

10.34119/bjhrv4n6-359 ◽

2021 ◽

Vol 4 (6) ◽

pp. 28084-28092

Author(s):

Alexandre Piccinini ◽

Matheus Henrique Oliveira De Sousa ◽

Robelli Cassia Santos Do Amaral ◽

Marta dos Santos Diniz Freitas ◽

Carlos Roberto de Menezes Peixoto ◽

...

Keyword(s):

Phenolic Compounds ◽

Volatile Compounds ◽

Volatile Compound ◽

Stingless Bees ◽

Antiradical Activity ◽

Rio Grande ◽

Qualitative Composition ◽

Rio Grande Do Sul ◽

Viscous Substance ◽

Plant Shoots

Propolis is a viscous substance produced and collected from plant shoots or exudates by worker bees. This study aimed to identify phenolic compounds, carotenoids, volatile compounds, and antiradical activity of propolis obtained from two stingless bees (SB), Scaptotrigona bipunctata (Tubuna) and Scaptotrigona depilis (Canudo), all native from Rio Grande do Sul. The two propolis showed a similar qualitative composition, but with quantitative differences. Catechin was the major phenolic compound found in propolis from S. bipuncata and S. depilis. The propolis from S. depilis showed a higher concentration of carotenoids, mainly lutein. The α-pinene was the volatile compound found in greater concentration in the two propolis.

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THE STUDY OF QUALITATIVE COMPOSITION AND DETERMINATION OF THE QUANTITATIVE CONTENT OF FLAVONOIDS IN RAW MATERIAL OF HELIOPHYSIS HELIANTHOIDES

Fitoterapia ◽

10.33617/2522-9680-2019-2-41 ◽

2019 ◽

Vol 2 (2) ◽

pp. 41-44

Author(s):

M. Yu. Pavlenko-Badnaoui ◽

◽

V. V. Protska ◽

I. O. Zhuravel ◽

I. G. Gurieva ◽

...

Keyword(s):

Raw Material ◽

Qualitative Composition ◽

Quantitative Content

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The study of the qualitative composition and the quantitative content of phenolic compounds in dietary supplements with lingonberry

Žurnal organìčnoï ta farmacevtičnoï hìmìï ◽

10.24959/ophcj.21.243782 ◽

2021 ◽

Vol 19 (4(76)) ◽

pp. 40-46

Author(s):

Oleksandr Yu. Maslov ◽

Mykola A. Komisarenko ◽

Serhii V. Kolisnyk ◽

Olga V. Antonenko ◽

Olena V. Kolisnyk ◽

...

Keyword(s):

Phenolic Compounds ◽

Dietary Supplements ◽

Total Content ◽

Hydroxycinnamic Acids ◽

Biologically Active ◽

Qualitative Composition ◽

Relevant Today ◽

Quantitative Content ◽

Layer Chromatography

Aim. Today, there are a lot of dietary supplements with lingonberry at the pharmaceutical market of Ukraine; therefore, the analysis and quality control of these products are relevant. In this connection, the aim of the research was to study the qualitative composition and determine the quantitative content of phenolic compounds in dietary supplements with lingonberry.Materials and methods. Such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM) were chosen for the study. The qualitative analysis was performed by thin layer chromatography (TLC), spectrophotometry was used for the quantitative determination.Results and discussion. Hydroquinone derivatives, flavonoids and hydroxycinnamic acids were found in the dietary supplements analyzed. The total content of phenolic compounds was 8.70, 0.26, 0.30 %, flavonoids – 6.37, 0.15, 0.12 %, hydroxycinnamic acids – 0.94, 0.06, 0.13 %, and hydroquinone derivatives – 1.01, 0.04, 0.03 % in such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM), respectively. Conclusions. The qualitative and quantitative analysis of the dietary supplements with lingonberry analyzed has been performed. “Extract of lingonberry” (MEDAGROPROM) dietary supplement meets the requirements of the State of Pharmacopoeia of Ukraine 2.0, whereas “Lingonberry” (Danikafarm) and “Lingonberry nano” (LSS SYSTEM) do not. Based on the results of the study it can be concluded that the problem of compliance of dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

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ПОРІВНЯЛЬНЕ ВИВЧЕННЯ АМІНОКИСЛОТНОГО СКЛАДУ СУХИХ ПОРОШКІВ БІОМАСИ FLAMMULINA VELUTIPES, ЯКІ ОТРИМАНІ МЕТОДАМИ ПОВЕРХНЕВОГО ТА ГЛИБИННОГО КУЛЬТИВУВАННЯ

Фармацевтичний часопис ◽

10.11603/2312-0967.2015.1.3753 ◽

2015 ◽

Author(s):

Т. А. Буткевич ◽

В. П. Попович

Keyword(s):

Amino Acids ◽

Liquid Chromatography ◽

Amino Acid ◽

Submerged Culture ◽

Microbiological Synthesis ◽

Qualitative Composition ◽

Medicinal Mushroom ◽

Dry Powders ◽

Quantitative Content ◽

Static Conditions

Summary: qualitative composition and quantitative content of 17 amino acids, including 7 essential were defined in Flammulina velutipes medicinal mushroom’s biomass dry powders. Biomass powders were received in static conditions and by submerged culture.Key words: biomass, Flammulina velutipes, cultivation in static conditions, submerged culture, aminoacids, liquid chromatography. Introduction. The medicinal mushroom Flammulina (F.) velutipes is widely used in non-traditional and officinal Eastern medicine as immunomodulatory, antitumor, antioxidant, antihypertensive, antibacterial, antifungal, antiviral agent [5; 6].Polysaccharide content of F. velutipes biomass was identified in previous studies [7]. Continuing work on the study of bioactive substances of medicinal mushroom we conducted determination of biomass dry powders amino acid composition.Amino acids are widely used in medicine for the treatment of neuropsychiatric disorders, some diseases of the gastrointestinal tract and liver, for the prevention of atherosclerosis, to increase immunity and to inhibit tumor development, etc. [1 - 4].Thus, the purpose of this work was to determine and compare amino acids qualitative composition and quantitative content in F. velutipes biomass dry powders.Materials and methodsThe objects of our study were two types of medicinal mushroom F. velutipes biomass dry powders. They were obtained by two methods of microbiological synthesis – cultivation in static conditions and by submerged culture.Water suspension with waste of CO2-extraction – Amaranthus flour (60 g/l) as a submerged culture medium for F. velutipes biomass production was explored. Mycelia were grown in 500 ml of microbial mattress at 26 ±1°C and pH value 6,0. Biomass was dried in a vacuum freeze dryer Cryodos-500 (Terrasa,Spain).Amino acid composition was studied on an automatic amino acid analyzer T-339 («Mikrotechna», Prague, Czech Republic) by ion-exchange liquid chromatography at the laboratory of the Palladin Institute of biochemistry of the National Academy of Sciences of Ukraine.Results and discussion17 amino acids, including 7 essential (valine, isoleucine, leucine, lysine, methionine, threonine, phenylalanine), 2 conditionally essential (arginine and histidine) and 8 replaceable amino acids (alanine, aspartic acid, glycine, glutamic acid, proline, serine, tyrosine, cystine) were identified in F. velutipes biomass dry powders. Biomass dry powders obtained by different methods of microbiological synthesis (cultivation in static conditions and by submerged culture) do not differ in amino acids qualitative composition.However, the biomass dry powder of medicinal mushroom that was obtained in static conditions contains 1.41 times more amino acids (essential to 1.27 times more; replaceable – 1.46 times more).Conclusions1. Using ion-exchange liquid chromatography in F. velutipes biomass dry powders were identified 17 amino acids, including 7 essential, 2 conditionally essential and 8 replaceable ones. Biomass dry powders were obtained by cultivation in static conditions and by submerged culture. The dominant of the essential amino acids in both investigated powders are leucine, of replaceable ones – glutamic and aspartic acid.2. Cultivation of medicinal mushroom F. velutipes in static conditions allows to obtain in 1.41 times more amino acids compared to the submerged culture.

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DETERMINATION OF CARBOHYDRATES CONTENT IN GENTIANA CRUCIATA L. BY GC/MS METHOD

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021v13i1.39820 ◽

2021 ◽

pp. 124-128

Author(s):

LILIIA BUDNIAK ◽

LIUDMYLA SLOBODIANIUK ◽

SVITLANA MARCHYSHYN ◽

PAVLINA KLEPACH ◽

YANA HONCHARUK

Keyword(s):

Plant Material ◽

Raw Material ◽

Qualitative Composition ◽

Mass Spectral Library ◽

Spectral Library ◽

Mass Spectral ◽

Quantitative Content ◽

Free Carbohydrates ◽

Gentiana Cruciata

Objective: Thus, the aim of our research was to determine the qualitative composition and quantitative content of carbohydrates in the studied plant material with the prospect of its application as a medicinal plant raw material. Methods: The carbohydrates of the herb of Gentiana cruciata L. determined by GC/MS method. Identification of monosaccharides was based on comparing their retention times with retention times of standards of the mass spectral library NIST 02. Quantification was done by using sorbitol added to the sample. Results: The quantitative content of 4 free carbohydrates such as D-saccharose (38.39 mg/g), D-Pinitol (12.01 mg/g), D-glucose (10.05 mg/g) and D-fructose (1.69 mg/g) was established in the herb of Gentiana cruciata L. Also, this method established the qualitative composition and quantitative content of eight carbohydrates (monosaccharides and their derivatives after hydrolysis): D-glucose (29.66 mg/g), D-Pinitol (22.24 mg/g), L-arabinose (4.26 mg/g), D-galactose (3.55 mg/g), D-xylose (1.80 mg/g), L-rhamnose (1.49 mg/g), D-Dulcitol (0.76 mg/g) and D-mannose (0.44 mg/g). Conclusion: The results of the study showed that carbohydrates from the Gentiana cruciata L. can be used as important resources of new ingredients for the pharmaceutical industry.

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