PO-197 Effects of aerobic exercise training on F13A-mediated energy metabolism in mice

Objective Apelin, an adipokine and also a myokine, is involved in energy metabolism. F13A is an analog of apelin-13. In this study, we aim to investigate the effect of aerobic exercise on F13A-mediated energy metabolism in mice. Methods 20 C57BL/6J wild mice were randomly divided into 4 groups(n:5), namely saline control group(SC), saline exercise group(SE), F13A control group(FC), and F13A exercise group(FE). Mice were intraperitoneally injected with F13A(0.2 μmol/kg/day) or saline(15 μl/kg/day). Mice in the exercise group underwent 60 min/day treadmill running at a speed of 15 m/min with a slope of 5 º. After 2 weeks, the maximal oxygen uptake was measured and the running speed was adjusted to 20 m/min. The treadmill running continued 4 weeks. The mice were individually housed in a Comprehensive Lab Animal Monitoring System(Columbus Instruments, Columbus, OH, USA) between the 3rd and 4th week of training with free access to food and water. O2 consumption(VO2), CO2 production(VCO2) and respiratory exchange ratio(RER) during a 24-h period were measured after 24h of acclimatization. Glucose oxidation(in g/min/kg0.75=[(4.545×VCO2)−(3.205×VO2)]/1000), and lipid oxidation(in g/min/kg0.75=[1.672×(VO2−VCO2)]/1000) were calculated. Results F13A alone increased glucose oxidation(P<0.01, vs SC group). Exercise plus F13A caused a significant decline in RER(P<0.01 vs FC and P<0.05 vs SE group), glucose oxidation(P<0.001 vs FC and P<0.05 vs SE group), whereas it increased lipid oxidation(P<0.05 in comparison with FC group). Exercise alone has no in fluence on 4 groups. Conclusions These findings suggest that 4 weeks aerobic exercise can regulate F13A reduce RER in mice, with a decrease of glucose oxidation and an increase of lipid oxidation in vivo.

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β-endorphin response to aerobic and anaerobic exercises in Wistar male rats

Medical Journal of Indonesia ◽

10.13181/mji.oa.203569 ◽

2020 ◽

Vol 29 (3) ◽

pp. 245-9

Author(s):

Rostika Flora ◽

Mohammad Zulkarnain ◽

Sukirno

Keyword(s):

Physical Exercise ◽

Aerobic Exercise ◽

Brain Tissue ◽

Exercise Group ◽

Anaerobic Exercise ◽

Treadmill Running ◽

Male Rats ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Aerobic And Anaerobic

BACKGROUND Physical exercise is strongly associated with the release of β-endorphin. It is assumed that the type and intensity of physical exercise contributes to the release of β-endorphin. This study aimed to compare levels of β-endorphin in brain tissue in response to aerobic and anaerobic physical exercise. METHODS This study was an experimental laboratory study using 35 male Wistar rats divided into one control group and two physical exercise treatment groups: aerobic and anaerobic. Physical exercise was conducted on an animal treadmill running at aspeed of 20 m/min for 30 min of aerobic exercise and 35 m/min with 1-min intervals every 5 min for 20 min for anaerobic exercises. Each aerobic and anaerobic exercise group was furtherly classified into three subgroups (1×/week, 3×/week, and 7×/week). β-endorphin levels were determined using enzyme-linked immunosorbent assay. The data were analyzed using independent t-test and one-way analysis of variance. RESULTS The highest mean of β-endorphin level was found in the weekly exercise (54.45 [1.41] pg/ml) of aerobic exercise group and daily exercise (70.50 [11.67] pg/ml) of anaerobic exercise group. Mean of β-endorphin level in control group was 33.34 (3.54) pg/ml. A significant increased of β-endorphin mean level (p<0.001) was found in all aerobic and anaerobic exercise groups except the aerobic exercise 7×/week group(37.37 [6.30] pg/ml) compared to control. CONCLUSIONS Both aerobic and anaerobic physical exercise conducted for 6 weeks could increase the level of β-endorphin in brain tissue.

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Brain Structure, Cardiorespiratory Fitness, and Executive Control Changes after a 9-Week Exercise Intervention in Young Adults: A Randomized Controlled Trial

Life ◽

10.3390/life11040292 ◽

2021 ◽

Vol 11 (4) ◽

pp. 292

Author(s):

Lina Zhu ◽

Qian Yu ◽

Fabian Herold ◽

Boris Cheval ◽

Xiaoxiao Dong ◽

...

Keyword(s):

Young Adults ◽

Aerobic Exercise ◽

Cardiorespiratory Fitness ◽

Executive Control ◽

Brain Structure ◽

Grey Matter ◽

Exercise Intervention ◽

Exercise Group ◽

Control Group ◽

Younger Adults

Cardiorespiratory fitness (CRF) is assumed to exert beneficial effects on brain structure and executive control (EC) performance. However, empirical evidence of exercise-induced cognitive enhancement is not conclusive, and the role of CRF in younger adults is not fully understood. Here, we conducted a study in which healthy young adults took part in a moderate aerobic exercise intervention program for 9 weeks (exercise group; n = 48), or control condition of non-aerobic exercise intervention (waitlist control group; n = 72). Before and after the intervention period maximal oxygen uptake (VO2max) as an indicator of CRF, the Flanker task as a measure of EC performance and grey matter volume (GMV), as well as cortical thickness via structural magnetic resonance imaging (MRI), were assessed. Compared to the control group, the CRF (heart rate, p < 0.001; VO2max, p < 0.001) and EC performance (congruent and incongruent reaction time, p = 0.011, p < 0.001) of the exercise group were significantly improved after the 9-week aerobic exercise intervention. Furthermore, GMV changes in the left medial frontal gyrus increased in the exercise group, whereas they were significantly reduced in the control group. Likewise, analysis of cortical morphology revealed that the left lateral occipital cortex (LOC.L) and the left precuneus (PCUN.L) thickness were considerably increased in the exercise group, which was not observed in the control group. The exploration analysis confirmed that CRF improvements are linked to EC improvement and frontal grey matter changes. In summary, our results support the idea that regular endurance exercises are an important determinant for brain health and cognitive performance even in a cohort of younger adults.

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Skeletal muscle lipid peroxidation in exercised and food-restricted rats during aging

Journal of Applied Physiology ◽

10.1152/jappl.1989.67.1.69 ◽

1989 ◽

Vol 67 (1) ◽

pp. 69-75 ◽

Cited By ~ 33

Author(s):

J. W. Starnes ◽

G. Cantu ◽

R. P. Farrar ◽

J. P. Kehrer

Keyword(s):

Lipid Peroxidation ◽

Gastrocnemius Muscle ◽

Thiobarbituric Acid ◽

Exercise Group ◽

Alpha Tocopherol ◽

Treadmill Running ◽

Control Group ◽

Muscle Lipid ◽

Fischer 344 ◽

Skeletal Muscle Lipid

The effects of chronic endurance exercise and food restriction on nonenzymatic lipid peroxidation (LP) of gastrocnemius muscle during aging were studied in male, Fischer 344 rats. One set of rats aged 6 and 18 mo were assigned to an exercise group (treadmill running) or an age-matched sedentary control group. After 6 mo (at the ages of 12 and 24 mo), LP and levels of alpha-tocopherol and its oxidized form, alpha-tocopheryl quinone, were measured. The extent of LP was determined in homogenates by measuring the content of thiobarbituric acid-reactive substances. After homogenization, the muscles were immediately evaluated for basal LP and also incubated in the presence of oxidant stressors for 2 h to assess antioxidant capacity (AOC) and for 24 h to estimate total peroxidizable lipid (TPL). Basal LP was not affected by age or exercise. AOC was not affected by exercise at either age. However aging significantly decreased AOC and increased alpha-tocopheryl quinone in both sedentary and exercised groups. TPL was not affected by age, but was increased by exercise training (P less than 0.05). Another set of rats was divided into the following three groups at 3 mo of age: sedentary, fed ad libitum (S); sedentary, caloric restricted by alternate day feeding (R); and exercised by forced treadmill running (E). Two years later, when the rats were 27 mo of age, the extent of LP was assessed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Aerobic Exercise Inhibits CUMS-Depressed Mice Hippocampal Inflammatory Response via Activating Hippocampal miR-223/TLR4/MyD88-NF-κB Pathway

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17082676 ◽

2020 ◽

Vol 17 (8) ◽

pp. 2676 ◽

Cited By ~ 1

Author(s):

Honglin Qu ◽

Ruilian Liu ◽

Jiaqin Chen ◽

Lan Zheng ◽

Rui Chen

Keyword(s):

Inflammatory Response ◽

Aerobic Exercise ◽

Exercise Group ◽

Inflammatory Factors ◽

Control Group ◽

Mild Stress ◽

Hippocampal Function ◽

Model Group ◽

Mice Model ◽

Protein Levels

Objective: To investigate the role of aerobic exercise in inhibiting chronic unpredictable mild stress (CUMS) depressed mice hippocampal inflammatory response and its potential mechanisms. Methods: Fifty-four male eight-week-old C57BL/6 mice were divided as control group (CG) (18 mice) and model group (36 mice). Model group mice were treated with 13 chronic stimulating factors for 28 days to set up the CUMS depression model. Neurobehavioral assessment was performed after modeling. The mice in the model group were randomly divided into the control model group (MG) and the aerobic exercise group (EG), with 18mice in each group. The EG group carried out the adaptive training of the running platform: 10 m/min, 0° slope, and increased by 10 minutes per day for 6 days. The formal training was carried for 8 weeks with 10 m/min speed, 0° slope, 60 min/d, 6 d/Week. After the training, a neurobehavioral assessment was performed, and hippocampus IL-1β and IL-10 protein levels were detected by ELISA. RT–PCR was used to detect the expression of miR-223 and TLR4, MyD88, and NF-κB in the hippocampus. Western blot was used to detect the expression of TLR4 and phosphorylated NF-κBp65 protein in the hippocampus. Results: The hippocampus function of CUMS depression model mice was impaired. The forced swimming and forced tail suspension time were significantly prolonged, and inflammatory factors IL-1β were significantly increased in the hippocampus. Aerobic exercise significantly improves CUMS-depressed mice hippocampal function, effectively reducing depressive behavior and IL-1β levels, and increasing IL-10 levels. Besides, aerobic exercise significantly upregulates the expression level of miR-223 and inhibits the high expression of TLR4, MyD88, and NF-κB. Conclusion: Aerobic exercise significantly increases the CUMS-depressed mice hippocampus expression of miR-223, and inhibits the downstream TLR4/MyD88-NF-κB signaling pathway and the hippocampal inflammatory response, which contributes to the improvement of the hippocampal function.

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Grape juice and aerobic exercise on blood pressure

Nutrition & Food Science ◽

10.1108/nfs-08-2019-0256 ◽

2019 ◽

Vol 50 (5) ◽

pp. 987-998 ◽

Cited By ~ 1

Author(s):

Juliane Barroso Leal ◽

Juçara Barroso Leal ◽

Joaline Barroso Portela Leal ◽

Yan de Lima Borges ◽

Maria Ivone Leal de Moura ◽

...

Keyword(s):

Blood Pressure ◽

Physical Exercise ◽

Aerobic Exercise ◽

Diastolic Pressure ◽

Systolic Pressure ◽

Grape Juice ◽

Exercise Group ◽

Control Group ◽

Content Type ◽

Before And After

Purpose This paper aims to verify the effect of 12 weeks of grape juice (GJ) consumption associated with aerobic exercise on the variation of the hypertensive elderly pressure. Design/methodology/approach A total of 45 hypertensive elderly of both sexes were distributed into: control group (CG, n = 10), exercise group (EG, n = 10), juice group (JG, n = 12) and juice and exercise group (JEG, n = 13). Blood pressure and heart rate were checked weekly before exercise in JG and JEG, and before and after intervention in all groups, with JG and JEG supplemented with 200 mL of GJ. Three weekly sessions of moderate walking were applied. Findings There was a reduction in EG, JG and JEG for systolic pressure and diastolic only for JG and JEG. The GJ consumption to the practice of aerobic exercise provided reductions in the arterial pressure of hypertensive, in addition to stabilization of the diastolic pressure. Research limitations/implications Although the objective of the study was to compare the effect and value of intervention with controls, the study had no intervention in food consumption, which could have led to more significant results. There was a limitation in the control drink, leading the study not to be blind, which may have impaired the results. However, it is probably not a bias, as the groups were divided by residence area, and therefore, had no direct contact with the other groups. Another limitation was that the sample size was still small, which would lead to more reliable results. Finally, although the existing limitations cannot be disregarded, the results of this research are very promising, especially when the objective is the effect of GJ and aerobic exercise on blood pressure, with the possibility of implementing supplemental GJ and the inclusion or not of exercise to the hypertensive elderly. Originality/value The paper deals with the benefits of GJ consumption associated with aerobic physical exercise on the blood pressure of elderly hypertensive patients. Considering that GJ along with physical exercise was enough to reduce the blood pressure of hypertensive elderly, this may be a new model to be used to reduce and/or control blood pressure, and GJ and the exercise to be part of the daily life of the population.

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A Preliminary Examination of Aerobic Exercise Effects on Resting EEG in Children With ADHD

Journal of Attention Disorders ◽

10.1177/1087054714554611 ◽

2014 ◽

Vol 21 (11) ◽

pp. 898-903 ◽

Cited By ~ 9

Author(s):

Chung-Ju Huang ◽

Chin-Wen Huang ◽

Yu-Jung Tsai ◽

Chia-Liang Tsai ◽

Yu-Kai Chang ◽

...

Keyword(s):

Aerobic Exercise ◽

Exercise Group ◽

Control Group ◽

Group 14 ◽

Children With Adhd ◽

Preliminary Examination ◽

Adhd Children ◽

Central Brain ◽

Before And After ◽

Eeg Pattern

Objective: This study attempted to determine whether the effects of physical exercise were reflected in the resting electroencephalographic (EEG) pattern of ADHD children. Method: Thirty-two ADHD children were assigned to either an exercise group or a control group. The exercise group participated in a water aerobics program for 8 weeks, whereas no intervention was administered to the control group. Resting EEGs were recorded under open-eyes condition before and after the intervention. Data from eligible participants, 15 from the exercise group (11 boys and 4 girls, 7.93 ± 1.02 years) and 14 from the control group (14 boys, 8.27 ± 1.04 years), were further analyzed. Results: While controlling for the baseline resting EEG, separate ANCOVAs indicated that the exercise group showed smaller theta/alpha ratios over the frontal and central brain sites after the intervention compared with the control group. Conclusion: This finding suggests that aerobic exercise may enhance the cognitive functions of children with ADHD, as reflected in resting EEG.

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PO-221 Effects of different motor functional therapies on cognitive impairment in type 2 diabetes mellitus

Exercise Biochemistry Review ◽

10.14428/ebr.v1i5.9523 ◽

2018 ◽

Vol 1 (5) ◽

Author(s):

Yu Zhou

Keyword(s):

Type 2 Diabetes ◽

Body Weight ◽

Cognitive Impairment ◽

Oxygen Uptake ◽

Elderly Patients ◽

Resistance Exercise ◽

Aerobic Exercise ◽

Exercise Group ◽

Control Group

Objective The clinical manifestations of diabetic cognitive dysfunction are decreased visual spatial ability, executive dysfunction, mental activity speed and attention, and decreased abstract logical reasoning. Cognitive dysfunction is irreversible. Timely and accurate assessment and diagnosis, early detection and intervention to delay disease progression are particularly critical. The Cognitive Assessment Scale plays an important role in screening for cognitive dysfunction in diabetes. To observe the effects of motor functional therapy on cognitive impairment and blood glucose, blood lipids, body weight, body composition, and maximal oxygen uptake in patients with type 2 diabetes. Methods A total of 63 elderly patients with type 2 diabetes mellitus in Chengdu community aged ≥65 years and educated for ≥12 years were enrolled in the motor function therapy group according to the simple mental state examination (MMSE) score (diabetes cognitive impairment, MMSE total score) ≥ 20 points, aerobic exercise group, n = 21) and functional function therapy group (diabetes cognitive impairment, MMSE total score ≥ 20 points, resistance exercise group, n = 21). The adult community of diabetes in Chengdu (normal cognitive function) with age ≥ 65 years, education time ≥ 12 years, and MMSE total score ≥ 24 points was used as the control group (n=21). Montreal Cognitive Assessment Scale (MOCA): MOCA is an assessment tool developed by Nasreddine and clinically proven to be used for rapid screening of MCI. There were no statistical differences in baseline data (age, gender, and hypertension incidence) among the 3 groups of patients. The LOTCA scale was used to evaluate the cognitive function of the subjects and to compare between groups. Sixty-three patients with type 2 diabetes were randomly divided into aerobic exercise intensity group (50% VO2max) (n=21), resistance exercise intensity group and control group (n=21). Both the aerobic exercise group and the resistance exercise group underwent a 12-week moderate-intensity exercise three times a week. The resistance middle strength group was trained 2 groups each time, each group was 25 minutes, the group rested for 5 minutes, 55 minutes in total, and the aerobic medium intensity group continued to exercise for 55 minutes. All patients underwent fasting blood glucose (FPG), glycosylated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), body weight, and fat weight (FW) before and after training. , lean body mass (LBM), maximal oxygen uptake (VO2max) determination; measure the energy expenditure of word movement and the total energy expenditure of the entire exercise process. Results There were no significant differences among the three groups before the test (P>0.05). All the indexes of the medium-intensity resistance exercise group and the medium-intensity aerobic exercise group were significantly different from the control group (P<0.05). After the MOCA score test There was an improvement in the score before the trial, and the total score of LOTCA was significantly different among the three groups. In addition to perceptual sub-items (item identification), the aerobic exercise group and the resistance exercise group LOTCA scale in perceptual sub-projects (incomplete object recognition), visual motion organization and its sub-projects (copying two-dimensional graphics, building blocks) The design and puzzle) project scores were higher than before the test, close to the control group score. The scores of the two groups of exercise therapy in the thinking operation and its sub-projects (Riska organized shape classification, picture arrangement B and geometric reasoning) and attention-focused items were lower than the control group. Compared with the resistance exercise, there was a significant difference in the maximum oxygen uptake between aerobic exercise and resistance exercise (P<0.05). Compared with the total exercise energy consumption in the first 6 weeks, the aerobic exercise group was superior to the resistance exercise group. The total exercise energy expenditure was compared in the last 6 weeks, and the resistance exercise group was superior to the aerobic exercise group. Conclusions Elderly patients with type 2 diabetes may have cognitive impairment earlier. In the absence of differences in exercise, the aerobic exercise group and the resistance exercise group improve cognitive impairment, blood sugar, blood fat, body weight, and body composition in patients with type 2 diabetes. There is no significant difference. Compared with MMSE, LOTCA has the advantage of identifying early cognitive impairment in elderly patients with type 2 diabetes and distinguishing the severity of the damage.

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Aerobic exercise training reduces blood pressure, angiotensin II and oxidative stress of patients with resistant hypertension: the EnRiCH trial

European Heart Journal ◽

10.1093/eurheartj/ehab724.2392 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

Author(s):

S Lopes ◽

J Mesquita-Bastos ◽

M Teixeira ◽

D Figueiredo ◽

J Oliveira ◽

...

Keyword(s):

Oxidative Stress ◽

Angiotensin Ii ◽

Exercise Training ◽

Aerobic Exercise ◽

Resistant Hypertension ◽

Exercise Group ◽

Aerobic Exercise Training ◽

Control Group ◽

Exercise Training Program ◽

And Oxidative Stress

Abstract Introduction Resistant hypertension is a major challenge of modern cardiovascular medicine, as it is a puzzling problem without a clear solution. Exercise training clearly reduces blood pressure (BP) and oxidative stress in patients with hypertension, however evidence is limited regarding resistant hypertension. Purpose To determine the effect of an aerobic exercise training program in BP, angiotensin II and oxidative stress in patients with resistant hypertension. Methods EnRicH is a prospective, two-center, single-blinded, randomized controlled trial with a parallel two-arm group. Sixty patients with resistant hypertension were randomly assigned in a 1:1 ratio to undergo a 12-week aerobic exercise training program (exercise) or usual care (control). The powered primary efficacy measure was 24-hour ambulatory systolic BP change from baseline. Secondary outcome measures included daytime and nighttime ambulatory BP, office BP, cardiorespiratory fitness, and oxidative stress and inflammatory biomarkers: Interferon-gamma (IFN-y), Angiotensin II, vascular endothelial growth factor (VEGF), and superoxide dismutase (SOD). Results Fifty-three patients (exercise n=26, control n=27) completed the study. Patients were mainly women (54.7%), with an office BP of 140.7±15.9/84.2±9.4 mm Hg and taking an average of 4.6 antihypertensive medications (median, 5; range, 3 to 7). At baseline, no differences were found between groups for the study outcomes and patient characteristics. Ambulatory systolic BP was reduced −7.1 mm Hg (95% CI, −12.8 to −1.4; P=0.015) in the exercise group (127.4±12.2 to 121.2±12.2, p=0.007) compared to control group (126.1±17.2 to 126.9±15.2, p=514) over 24-hour. In addition, 24-hour ambulatory diastolic BP (−5.1 mm Hg, −7.9 to −2.3, P=0.001), daytime ambulatory systolic (−8.4 mm Hg, −14.3 to −2.5, P=0.006), and diastolic BP (−5.7 mm Hg, −9.0 to −2.4, P=0.001) were also reduced in the exercise group compared to the control group. There were no differences in the change of nighttime ambulatory BP between groups. Cardiorespiratory fitness improved in the exercise group by 14% (4.7 ml.kg-1.min-1, P<0.001), while it remained unchanged in the control group (−0.37 ml.kg-1.min-1, P=0.442). A significant between-group difference in favor of exercise group was found for IFN-y (−4.3 pg/mL, 95% CI: −7.1 to −1.5; P=0.003), Angiotensin II (−157.0 pg/mL, 95% CI: −288.1 to −25.9; P=0.020), VEGF (10.53 pg/mL, 95% CI: 0.60 to 22.54; P=0.035), and SOD (0.35 pg/mL, 95% CI: 0.10 to 0.58; P=0.009). Conclusions A 12-week moderate intensity aerobic exercise program reduced ambulatory BP, angiotensin II and oxidative stress in patients with resistant hypertension. The antihypertensive effects of exercise in patients with resistant hypertension may be mediated by positive changes in oxidative stress and inflammatory biomarkers. FUNDunding Acknowledgement Type of funding sources: Foundation. Main funding source(s): European Union through European Regional Development Fund – Operational Competitiveness Factors Program (COMPETE)Portuguese Government through FCT - Foundation for Science and Technology

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PO-198 Effect of aerobic exercise on antioxidation ability of mice kidneys

Exercise Biochemistry Review ◽

10.14428/ebr.v1i4.13073 ◽

2018 ◽

Vol 1 (4) ◽

Author(s):

Sisi He ◽

Haitao Wang

Keyword(s):

Aerobic Exercise ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Kidney Tissue ◽

Exercise Group ◽

Control Group ◽

Adult Group ◽

Total Antioxidant ◽

The Difference ◽

Control Group Group

Objective To study the total antioxidant capacity of the kidney in the Aged mice Total Antioxidant Capacity(T-AOC), Malondialdehyde (MDA) and superoxide dismutase (SOD) expression level by aerobic exercise. Objective to explore the effect of aerobic exercise on antioxidation ability of mice. Methods 30 Male C57BL/6 strain mice were randomly divided into adult group ( group CC) in the 13 months old, in the control group (Group C), 5 months of quiet feeding, the aerobic exercise group (Group E) ran for 5 months, 10 per group, and the adult group (group CC) compared with the control group (Group C), The Analysis control Group (Group C) was compared with the aerobic exercise group (Group E). After 5 months of Treadmill running in Group E, Determination of antioxidant index of each group. The statistical method is processed by SPSS20.0 statistic software, the experimental data is expressed by the mean ± standard deviation, the two mean numbers are analyzed by independent sample t test, and the difference of (p<0.05) is statistically significant. Results T-AOC of adult Group was (0.99±0.13) U/mg Prot, control group was (0.27±0.07) u/mg prot, aerobic exercise group (0.39±0.10) U/mg prot, adult group SOD as (46.32± 1.84) U/mg, control Group (29.71±7.64) U/mg, aerobic exercise group (39.85±5.83) U/mg, adult group MDA (8.15±0.16) nmol/mg, control Group (8.83±0.26) nmol/mg, The aerobic exercise group was (6.31±1.23) nmol/mg, the adult group was SOD/MDA (5.69±0.32), the control group was (3.38±0.9) and the aerobic exercise group was (6.55±1.08). Compared with the adult group, the control group T-AOC and SOD decreased significantly, MDA significantly increased, the difference was very significant (p<0.01), sod/mda significantly decreased, SOD/MDA difference was significant (p<0.01). Compared with the control group, the aerobic exercise group T-AOC and SOD obviously increased, MDA significantly decreased, the difference was significant (p<0.01), SOD/MDA increased significantly, the difference was very significant (p<0.01). Conclusions To sum up, with the increase of the age of mice MDA expression is increased, the 18 months old SOD expression is lower than 13 months old, indicating that the antioxidant capacity with the increase in age, The results of this study are consistent with the results of previous scholars. The results show that aerobic exercise has some antioxidant effect, and aerobic exercise can decrease expression of kidney tissue SOD, raise the expression of kidney tissue T-AOC, increase the expressionof kidney tissue, increase the expression of SOD/MDA, and reduce the oxidative stress of kidney to some extent, So as to protect the role of the kidney, aerobic exercise and kidney-related mechanisms also need our further study.

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PO-259 Effect of 30%，50% and 70% VO2 max treadmill exercises on gut microbiome in hypertensive mice

Exercise Biochemistry Review ◽

10.14428/ebr.v1i5.11073 ◽

2018 ◽

Vol 1 (5) ◽

Author(s):

Jin Li ◽

Wenting Shi ◽

Pengyi Zhang

Keyword(s):

Blood Pressure ◽

Aerobic Exercise ◽

Gut Microbiome ◽

Pathogenic Bacteria ◽

Exercise Group ◽

The Body ◽

National Standard ◽

Control Group ◽

Beneficial Bacteria ◽

Vo2 Max

Objective Gut microbiome has a significant impact on human health through the interaction with host and environment, which is closely related to a series of chronic diseases. The diversity of gut microbiome and its metabolic disorder are the risk factor of hypertension. The changes of gut microbiome structure and abundance are closely related to the pathogenesis of hypertension, in which Bifidobacterium and lactic acid bacteria can bind with the hypotensive substances to show the hypotensive therapy. However, the diet and exercise have great impact on the structure and function of gut microbiome, and of which aerobic exercise could increase the ratio of gut beneficial bacteria to harmful bacteria effectively. The effect of treadmill exercise on gut microbiome of hypertensive mice was studied in this paper, which provided a theoretical basis for the prevention and treatment of hypertension by gut microbiome. Methods SPF Kunming mice were fed with 8% high salt diet for 6 weeks to make the hypertension model. Compared with the blood pressure of mice before the formal experiment, The standard was that the blood pressure of the experimental mice was increased by SBP >15% or DBP > 5%, which was indicated the model of hypertension was established successfully. The motion slope and velocity of the maximum oxygen uptake of 30%, 50%, and 70% were measured respectively. The mice were divided into 4 groups randomly according to their body weight, 10 mice/group. The 30% VO2 max, 50% VO2 max, and 70% VO2 max were exercised for 6 weeks as well as the control group. The mice were divided into cages and fed in accordance with the national standard rodent diet. The blood pressure of mice was measured weekly by tail pressure method (Tail-cuff).The abundance of Bifidobacterium, Bacteroides, Lactobacillus and Enterobacter in the gut microbiome of mice feces were tested by 16S sequencing every two weeks. The ratio of Firmicute / Bacteroides (F/B ratio) was also measured by sequencing as a parameter to reflect the disorder of gut microbiome. The ratio of Firmicutes / Bacteroides is almost equal 1, and the present study found that the F/B increased significantly in the hypertensive group. Real-time PCR was used to detect the changes of plasma inflammatory factors IL-1β, IL-6 and TNF-ɑ. The previous study had shown that the intestinal disorders can lead to an increase in pathogenic bacteria, further leading to the inflammation. Finally, the experimental data were analyzed by independent sample t-test. Results (1) After six weeks of exercise intervention, the blood pressure (132.87mm Hg±5.23mm Hg) of the exercise group was significantly lower than the control group (99.57mm Hg±7.47mm Hg), especially in the 50%VO2 max mice. (2) Compared with the rest group, the abundance of gut microbiome in the exercise group was increased, among which the number of Bifidobacteria, Lactobacillus, and Bacteroides were increased significantly, of which 50% of VO2 max group increased most significantly, and the number of Enterobacter was less than the control group (p<0.05). (3) Compared with the control group, the ratio of F/B in the exercise groups were lower than the control group, but the 50% VO2 max group was decreased most obviously (p < 0.05). (4) Compared with the control group, the plasma levels of IL-1β, IL-6 and TNF-ɑ in exercise groups were significantly lower than the control group (p < 0.05). Conclusions (1) The aerobic exercise could change the abundance and structure of gut microbiome in hypertensive mice, increase the beneficial bacteria Bifidobacteria and lactobacillus in the intestinal tract of mice, and reduce the ratio of Firmicutes/Bacteroides, improving the gut microbiome disorder. (2) The aerobic exercise could alleviate the inflammatory reaction of the body by regulating the structure of gut microbiome of hypertensive mice, improving the blood pressure of mice; (3) 50%VO2 max was the most significant exercise intensity to improve the abundance and structure of gut microbiome in hypertensive mice.

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