scholarly journals The effect of submersion denture base acrylic resin in a betel leaf ekstract solution against growth candida albicans

2017 ◽  
Vol 2 (3) ◽  
pp. 183
Author(s):  
Ardiansyah S. Pawinru ◽  
Andi Izham
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Leaf Extract ◽  
Longitudinal Design ◽  
Base Material ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Betel Leaf ◽  
Experimental Laboratory

Objective: Microorganisms are often found in the oral cavity is candida albicans approximately 40 % in the oral cavity. Candida albicans can penetrate the acrylic resin that can infect the soft tissue and is the cause of denture stomatitis therefore the disinfection pf denture base is an important factor that must be done. The purpose of the research is to determine how the effect of submersion denture base acrylic resin in a betel leaf exstract solution against growth candida albicans.Material and Methods: This research is an experimental laboratory with a longitudinal design (follow-up) study. The Sampling method used is Total Sampling. This study used a sample of 12 pieces of denture base material acrylic resin to be suspended candida albicans fungus for 1x24 hours at 37oC.Results: The number of candida albicans colonies n denture base acrylic resin which soaked betel leaf extract solution that the dilution 10-1with consentration 2.5% total colony count is 2 and the results 2.0 x 101 CFU/ml , on a control solution that the dilution 10-2 total colony 355 and the result 3.55x104 CFU/ml , that the dilution  10-3 total colony 62 and the result 6.2x104 CFU/ml.Conclusion: Betel leaf extract (piper linn) can inhibit the growth of candida albicans on denture base of acrylic resin, betel leaf extract (piper linn) 5% is a minimal anti-fungal power that can be used as a disinfectant solution in acrylic resin denture.

scholarly journals Disinfection Effect of 10% Ricinus Communis Oil on Candida Albicans Counts of Heat Polymerized Acrylic Resin

2021 ◽  
Vol 24 (1) ◽  
pp. 6-10
Author(s):  
Putri Welda Utami ◽  
Nurdiana
Keyword(s):  
Candida Albicans ◽  
Ricinus Communis ◽  
Base Material ◽  
Acrylic Resin ◽  
Biological Properties ◽  
Chlorhexidine Gluconate ◽  
P Value ◽  
Denture Base ◽  
Post Test ◽  
Experimental Laboratory

Dentures is used to replace tooth loss. The most used denture base material is acrylic resin. The biological properties of acrylic resins give microorganisms the ability to colonize. Certain type of microorganism often found at the dentures base is Candida albicans. Denture’s disinfection is usually used to reduce Candida albicans. One of natural source that now is being widely researched is Ricinus communis oil. The purpose of this study was to analyze disinfection effect of Ricinus communis oil 10% on Candida albicans counts on heat polymerized acrylic resin. This research is an experimental laboratory study with a post-test only design. Samples was heat polymerization acrylic resin plates measuring 10 x 10 x 1 mm were made in the Department of Prosthodontic Faculty of Dentistry, Universitas Sumatera Utara with 10 plates for each for 10% Ricinus communis oil group and 0.2% chlorhexidine gluconate group (control). The Candida albicans colonies count was carried out in the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara using colony counter. The data obtained was statistically count with the Kruskal-Wallis Test. The result showed the decrease in Candida albicans count with the highest value in the 10% Ricinus communis oil group is 9 x 100 CFU/ml and the highest value in the 0.2% chlorhexidine gluconate group is 0 x 100 CFU/ml. The Kruskal-Wallis test showed significant results with p value of 0.0001 (p <0.05) indicating there was a disinfection effect of 10% Ricinus communis oil and 0.2% chlorhexidine gluconate on Candida albicans count of heat polymerized acrylic resin. The study concluded that 10% Ricinus communis oil effective at decreasing Candida albicans count.

scholarly journals Titanium Dioxide Addition to Heat Polymerized Acrylic Resin Denture Base Effect on Staphylococcus aureus and Candida albicans

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Dwi Tjahyaning Putranti ◽  
Afrina Fadilla
Keyword(s):  
Staphylococcus Aureus ◽  
Titanium Dioxide ◽  
Candida Albicans ◽  
Tio2 Nanoparticles ◽  
Base Material ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Significant Difference ◽  
Almost All

  Introduction: Heat-polymerized acrylic resin is used to manufacture almost all denture bases. One of the disadvantages of acrylic resin is its porosity and surface roughness; food scraps attach easily. If the dentures are not cleaned, they become a place for microbial species development and cause denture stomatitis, with Staphylococcus aureus and Candida albicans as the major etiologic agents. Adding nanoparticles of titanium dioxide to heat-polymerized acrylic resin may provide antimicrobial activity to the acrylic resin base.   Objectives: This study aimed to determine whether adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base materials affected the amounts of Staphylococcus aureus and Candida albicans.   Methods: The samples in this study used heat-polymerized acrylic resin without the addition of TiO2 nanoparticles and with the addition of 2%, 3%, and 4% TiO2 nanoparticles on test bar sizes of 10 mm × 10 mm × 1 mm. There were 48 total test samples: 24 of Staphylococcus aureus and 24 of Candida albicans. The obtained data were analyzed by a one-way ANOVA test.   Results: The results showed that adding TiO2 nanoparticles influenced the amount of Staphylococcus aureus (p<0.05) and Candida albicans (p<0.05). A least significant difference test showed differences of effect in the amounts of Staphylococcus aureus when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base and there were differences in effect to amounts of Candida albicans when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base.   Conclusion: Adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base material affects the amounts of Staphylococcus aureus and Candida albicans.

scholarly journals Activity of Averrhoa Bilimbi L Leaf Extract as Denture Cleaner Towards The Number of Colonies of Candida Albicans in Heat Cured Resin with a 30 Minutes Period

2021 ◽  
Vol 7 (2) ◽  
pp. 402-407
Author(s):  
Idamawati ◽  
Rouli Natasia M. Simanjuntak ◽  
Molek ◽  
Listyaning Kartiko Kinasih
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Leaf Extract ◽  
Average Concentration ◽  
Dilution Method ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Chronic Inflammatory Condition ◽  
The One ◽  
And Control

Denture stomatitis is a chronic inflammatory condition of the oral mucosa thatgenerally occurs in users of removable dentures. The cause is multifactorialbut is often associated with the presence of Candida albicans. The mucosawhich is covered by the denture base also reduces the function of saliva whichfunctions as a cleanser for the oral cavity and results in food wasteaccumulating and microorganisms such as Candida albicans can increase innumber. Candida albicans is a normal flora of the oral cavity which is anopportunistic pathogen. The starfruit plant (Averrhoa billimbi l) is a naturalingredient that can be used as an antifungal agent. The aim was to determinethe ratio of the effectiveness of starfruit leaf extract (Averrhoa billimbi) betweenthe concentrations of 12.5%, 25%, 50%, 100%, control + (Clorhexidine) andcontrol - (Aquadest) in reducing the number of Candida albicans colonies. Thetest method used the dilution method, the colony count of Candida albicansusing a spectrophotometer. The results of the one way ANOVA statistical testresulted in a significance value of p = 0.000 (p <0.05) which means that thereis a difference in the average concentration of Candida albicans between theextract concentrations of 12.5%, 25%, 50%, 100%, control + and control. -namely 6.78 ± 0.35, 6.27 ± 0.21, 4.89 ± 0.29, 1.01 ± 0.18, 0.59 ± 0.19 and 9.13± 2.28 in inhibiting growth Candida albicans and this was confirmed by theTukey HSD posthoc test. The conclusion of this study is that there is adifference between the antifungal effectiveness of starfruit leaf extract(Averrhoa billimbi) concentrations of 12.5%, 25%, 50% and 100% in inhibitingthe growth of Candida albicans colonies.

scholarly journals Efektivitas Perasan Daun Kemangi (Ocimum basilicum) dibanding Larutan Pembersih Gigi Tiruan Effervescent sebagai Pembersih Gigi Tiruan Resin Akrilik terhadap Pertumbuhan Candida albicans

2019 ◽  
Vol 7 (2) ◽  
pp. 135
Author(s):  
Sherlika Puspita Sari ◽  
Achmad Gunadi ◽  
Dewi Kristiana
Keyword(s):  
Candida Albicans ◽  
Base Material ◽  
Acrylic Resin ◽  
Chemical Cleaning ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Treatment Groups ◽  
Cleaning Agents ◽  
The One ◽  
One Way Anova

The heat cured acrylic resin is common used as denture base material, but its microporosity can affect the cleanliness of the denture base lead to accumulation of plaque and food waste, and further increase the number of Candida albicans and cause denture stomatitis. There are 2 type of cleaning agents; natural cleaning agents, such as basil and chemical cleaning agents, for example effervescent artificial cleaning tablets. This study compared the effectiveness of basil leaves squeeze and effervescent denture cleaning solutions in soaking heat cured acrylic resin plates on the growth of C. albicans. There were 24 of 10x10x1 mm acrylic resin plates divided into 6 treatment groups. The acrylic resin plate was soaked in the basil leaves squeeze and effervescent denture cleaning solutions. Measurement of C. albicans absorbance used a spectrophotometer, then calculated the total of C. albicans using the formula. Data was analyzed using the One Way ANOVA test. The analysis showed that there are significant differences on the growth inhibition of C. albicans between treatment groups (p <0.005). The effervescent denture cleansing solution has a better antifungal effectiveness than the basil leaves

scholarly journals Comparison of Candida albicans colony amount in heat-cured acrylic and thermoplastic nylon resin after immersion in Ulee Kareng coffee (Coffea robusta)

2017 ◽  
Vol 29 (1) ◽  
Author(s):  
Iin Sundari ◽  
Ridha Andayani ◽  
Novriyanti Fatimah Harahap
Keyword(s):  
Candida Albicans ◽  
Base Material ◽  
Acrylic Resin ◽  
T Test ◽  
Test Results ◽  
Denture Base ◽  
Acrylic Resins ◽  
Experimental Laboratory ◽  
Coffea Robusta ◽  
Colony Counter

Introduction: Heat-cured acrylic resin is the most often used material in the manufacture of denture base. Along with the development of science and technology, to overcome the shortcomings of heat-cured acrylic resin, repairment of denture base material was done, one of them is a thermoplastic nylon resin. On the use of denture often found Candida albicans attached to the denture. This study aimed to determine the comparison of the amount of Candida albicans colony on heat-cured acrylic and thermoplastic nylon resin after immersed in the Ulee Kareng coffee (Coffea robusta). Methods: The number of specimens in this study were 8 specimens; 4 Meliodent® heat-cured acrylic resins and 4 Bio Tone® thermoplastic nylon resins with size of 10x10x2 mm. The methods of this study was experimental laboratory. The specimens were stored in a solution of Ulee Kareng coffee (Coffea robusta) for 7 days. Each specimen was contaminated with Candida albicans, then the number of Candida albicans colony was counted with Colony counter, from threshing results of heat-cured acrylic and thermoplastic nylon resins. Data were analyzed with unpaired t test. Results: Unpaired t test results showed that there were significant differences (p<0.05) between the number of colonies of Candida albicans in heat-cured acrylic resin (4.5 CFU/ml) and thermoplastic nylon resin (1.5 CFU/ml) after both immersed in the Ulee Kareng coffee (Coffea robusta). Conclusion: The amount of Candida albicans colony on heat-cured acrylic resin was higher than on thermoplastic nylon resin.

scholarly journals Denture Acrylic Resin Material with Antibacterial and Protein-Repelling Properties for the Prevention of Denture Stomatitis

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 230
Author(s):  
Salwa Omar Bajunaid ◽  
Bashayer H. Baras ◽  
Michael D. Weir ◽  
Hockin H. K. Xu
Keyword(s):  
Oral Mucosa ◽  
Pathological Condition ◽  
Base Material ◽  
Acrylic Resin ◽  
Control Group ◽  
Control Groups ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Colony Forming Unit ◽  
Bioactive Agents

Denture stomatitis is a multifactorial pathological condition of the oral mucosa that affects up to 72% of denture wearers. It is commonly seen on the palatal mucosa and characterized by erythema on the oral mucosa that are in contact with the denture surface. The aim of this study was to incorporate 2-methacryloyloxyethyl phosphorylcholine (MPC) and dimethylaminohexadecyl methacrylate (DMAHDM) into a high impact polymethylmethacrylate heat-cured denture base acrylic resin as a potential treatment for denture stomatitis. We used a comparative study design to examine the effect of incorporating MPC as a protein repellent agent and DMAHDM as an antifungal agent to prevent the adherence of Candida albicans to the denture base material. The dual incorporation of MPC and DMAHDM reduced C. albicans biofilm colony-forming unit by two orders of magnitude when compared to the control group devoid of the bioactive agents. Although the addition of MPC and DMAHDM alone or in combination significantly reduced the flexural strength of the material, they showed reduced roughness values when compared to control groups. This new denture acrylic resin provides the benefit of enhancing C. albicans biofilm elimination through dual mechanisms of action, which could potentially reduce the prevalence of denture stomatitis.

scholarly journals JUMLAH KOLONI CANDIDA ALBICANS PADA PLAT RESIN AKRILIK HEAT CURED SETELAH DILAKUKAN PERENDAMAN EKSTRAK DAUN KEMANGI (OCIMUM BASSILICUM LINN) 50%

2020 ◽  
Vol 16 (2) ◽  
pp. 61-63
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
Sintha Nugrahini
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Parametric Test ◽  
Group Design ◽  
Cleaning Methods

Accumulation of plaque and food scraps on an acrylic resin base will increase bacterial colonies and C. albicans which will cause denture stomatitis. Maintenance of denture hygiene using mechanical, chemical and combination of two methods. Basil leaf extract contain essential oils which are important in fight against resistant C. albicans biofilms. The purpose of this study was to determine the comparison of C. albicans colonies after cleaning various denture cleaning methods. The method of this study is used an experimental method, the study design is posttest only with control group design. Sample size of heat-cured acrylic resin plate is 40x12x3mm. this study use Kruskall-Wallis test and Mann-Whitney test as data analysis for comparison tests between groups (non-parametric test). The results showed that there were significant differences between the various cleansing methods used in reducing C. albicans colonies. The compotition of flavonoids basil leaf extract is anti-microbial which can prevent the entry of fungi that harm the body.

scholarly journals Chitosan 2% Effect on Prohibiting the Growth of Candida Albicans on Heatcured Acrylic Resin

2017 ◽  
Vol 6 (2) ◽  
pp. 17
Author(s):  
Angela Evelyna ◽  
Dahlia Sutanto ◽  
Elizabeth Tiffany
Keyword(s):  
Candida Albicans ◽  
Statistical Analysis ◽  
Natural Compound ◽  
Agar Medium ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Base Materials ◽  
Oral Tissue ◽  
The Difference

Maintaining acrylic denture hygiene is a necessary to prohibit the growth of several oral fungi such as Candida albicans. Candida albicans are the main etiological microorganism of denture stomatitis. One of the ways to clean denture is by immersion on synthetic denture cleaner liquid such as oxygenizing denture cleaners. However, synthetic denture cleaner have several limitation due to its biocompatibility on human body especially oral tissue. Chitosan is a derivate of chitin, a natural compound of sea creature exosceleton. Chitosan have antifungi and antibacterial nature. The objective of this study is to evaluate the antifungi efectifity of chitosan 2% on acrylic heatcured and compare it with oxygenizing denture cleaner. Aquades is used as a control. The sample consists of 15 acrylic heatcured bars (10x10x2 mm) prepared by contamination on Candida albicans suspensions at temperature 37°C for 24 hours and immersed on 3 different treatments wich are chitosan 2%, oxygenizing denture cleanser, and aquades as control and then, the solutions are being vibrate and put into sabouraud dextrose agar medium. The colony of Candida albicans counted manually. Result shows, highest mean of Candida albicans colony on aquades group (365,66 CFU/plate), followed by oxygenizing denture cleanser treatment (178,33 CFU/plate). The smallest number colony of Candida albicans are found at chitosan 2% treatment (45,33 CFU/plate). Statistical analysis using ANOVA one way shows F=9.826 and p=0, this means, there is minimal one pair of groups with very significant differential (p<0,01) which are chitosan 2% and oxygenizing denture cleanser compared to aquades as control. The difference between those two treatments also significant. Conclusions of this study is that chitosan 2% solution have a sifnificant effect on prohibiting the growth of Candida albicans colony on acrylic heatcured denture base materials. The effectivity of this solution is significantly higher than oxygenizing denture cleanser treatment.

scholarly journals Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 221
Author(s):  
Avi Meirowitz ◽  
Arkadi Rahmanov ◽  
Eti Shlomo ◽  
Helena Zelikman ◽  
Eran Dolev ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Candida Albicans ◽  
3D Printing ◽  
Oral Candidiasis ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Heat Curing ◽  
Vitro Effect

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

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