Effectiveness of Lactic Acid Bacteria to Improve Cyprinus carpio Fingerlings Resistance Against

2020 ◽  
Vol 5 (1) ◽  
pp. 138-152
Author(s):  
Rosidah Rosidah ◽  
Yuniar Mulyani ◽  
Walim Lili ◽  
Khasanatur Rosyidah
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Cyprinus Carpio ◽  
Clinical Symptoms ◽  
Challenge Test ◽  
The Body ◽  
Randomized Design ◽  
Body Resistance ◽  
Disease Attack

Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of  leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9%  and the highest survival rate was 80%. 

scholarly journals Effectiveness of lactic acid bacteria to increase the body resistance of common carp, Cyprinus carpio Linnaeus, 1758 against Aeromonas hydrophila

2021 ◽  
Vol 21 (3) ◽  
pp. 291-304
Author(s):  
Rosidah Rosidah ◽  
Yuniar Mulyani ◽  
Fittrie Mellianawaty Pratiwy ◽  
Afifah Shabirah ◽  
Juli A Sinaga
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Common Carp ◽  
Aeromonas Hydrophila ◽  
Clinical Symptoms ◽  
The Body ◽  
White Blood Count ◽  
Cell Counts ◽  
The Common

This study aims to find out the right lactic acid bacteria (LAB) isolates to improve the common carp body's immune system against the attack of the Aeromonas hydrophila bacterium. Lactic Acid Bacteria (LAB) isolates were obtained from the organs of the common carp intestine. The study used a Completely Randomized Design consisting of four treatments and three replications. The treatment used was immersion of test fish for 24 hours in several isolates of LAB with a density of 108 cfu ml-1, namely A: without LAB isolate (control), B: isolate CcB7, C: isolate CcB8, D: isolate CcB15. Immersion is done three times with a frequency of seven days. Parameters observed included clinical symptoms, survival rate, red and white blood cell counts. The results showed the immersion of LAB isolate CcB15, namely Lactobacillus gasseri was most effective for increasing the body's resistance to Common Carp, which was demonstrated by mild clinical symptoms and faster recovery, high fish survival rate (83.33%), and number of cells the highest white blood count of 147,47x103 cell mm-3 (an increase in white blood cells by 38%).

scholarly journals Respon Imun Spesifik Larva Ikan Mas (Cyprinus carpio) melalui Imunitas Maternal yang Diberi Vaksin Inaktif Whole Cell Aeromonas salmonicida

Biospecies ◽  
2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Syohibahttul Islamiyah BAHAR ◽  
Esti HARPENI ◽  
Eko EFFENDI
Keyword(s):  
Antibody Titer ◽  
Cyprinus Carpio ◽  
Clinical Symptoms ◽  
Aeromonas Salmonicida ◽  
The Body ◽  
Cell Vaccine ◽  
Agglutination Reaction ◽  
Whole Cell ◽  
Randomized Design ◽  
Vaccine Treatment

Aeromonas salmonicida are specific bacteria that can cause infections and death to the cultivation of carp (Cyprinus carpio) during larval stage. Death in carp can be prevented by a vaccine, but the vaccine can only be given on the seed over the age of 3 weeks. Maternal vaccination needs to be done to improve the immune system of the larvae by means of inactivated whole cell vaccine A. salmonicida on broodstock ready to spawn. Aims to determine the effectiveness of vaccines on breeders carp to the parent antibody titer test and larvae, as well Survival Rate (SR) and the Relative Percent Survival (RPS) larvae. This research was conducted with a completely randomized design, 4 treatments A (control); B (0.3 ml/kg); C (0.4 ml/kg); D (0.5 ml/kg) and 3 repetitions. The results show that the antibody titer of 0.3 ml dose capable of providing agglutination reaction to pitting 7th (64x dilution) in broodstock, and vaccine doses 0,4ml on broodstock able to give agglutination reaction to the larvae until all 6 wells (32x dilution). A dose of 0.4 ml/kg resulted the highest SR and RPS with 96.11% and 81.25% respectively. Clinical symptoms of redness in control larvae was spread throughout the body whereas on the vaccine treatment was only in certain body parts.Keywords: A. salmonicida, vaccines, maternal immunity, larva, specific immune respon 

scholarly journals The Effect of Potassium Diformate as Feed Additive on Immune Performances of Nilem (Osteochilus hasselti Valenciennes, 1842) Under Infection of Aeromonas hydrophila

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 11
Author(s):  
Ibnu Bangkit Bioshina Suryadi ◽  
Desty Nurul Ulfa ◽  
Ayi Yustiati ◽  
Rosidah Rosidah
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Experimental Method ◽  
Aeromonas Hydrophila ◽  
Clinical Symptoms ◽  
The Body ◽  
Effective Dosage ◽  
Feed Additive ◽  
Random Design ◽  
Body Resistance

This research aims to determine the most effective dosage of potassium diformate additives in feed to increase the body resistance of nilem carp (Osteochilus hasselti). This research used Complete Random Design (CRD) experimental method with 4 treatments and 4 replications. The treatment used was the addition of potassium diformate on feed (0 %, 0.1 %, 0.3 % and 0.5 %). After 28 days of treatment, nilem fingerlings were challenged with Aeromonas hydrophila bacteria with a density of 108 cfu ml-1. Parameters observed were the number of leukocytes, erythrocytes, survival rate, clinical symptoms and water quality. The result showed that leukocytes and erythrocytes number were significantly different in each treatment. Meanwhile, leukocytes and erythrocytes number of nilem fingerlings in challenged test were increased and decreased due to the addition of potassium diformate and A. hydrophila infection. Clinical symptoms after challenged test were relatively similar such as haemorhagic, ulceration, exopthalmia, dropsy and abnormal swimming movements. Therefore, survival rate of nilem fingerlings with 0.3 % of potassiun diformate was the highest compared to other treatments, namely 81.67 %.

scholarly journals Efektifitas bubuk rumput laut merah (Gracillaria sp) sebagai imunostimulan terhadap infeksi bakteri streptococcus iniae pada ikan lele dumbo (Clarias gariepinus)

2016 ◽  
Vol 3 (2) ◽  
pp. 81
Author(s):  
Cut Sofia Amanda ◽  
Eva Ayuzar
Keyword(s):  
Survival Rate ◽  
Bacterial Infection ◽  
Clinical Symptoms ◽  
Economic Value ◽  
Clarias Gariepinus ◽  
Challenge Test ◽  
Streptococcus Iniae ◽  
Red Seaweed ◽  
Randomized Design ◽  
Physiology Laboratory

Usaha budidaya ikan lele dumbo memiliki nilai ekonomis yang tinggi karena merupakan salah satu komoditas hasil perikanan yang potensial untuk diekspor. Penelitian ini dilaksanakan pada Bulan Mei hingga Bulan Juni 2015 dan dilaksanakan di Laboratorium Fisiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Laboratorium Akuatik Fakultas Kedokteran Hewan Universitas Syiah Kuala dan laboratorium Mikrobiologi BBI Ujong Batee Aceh Besar. Tujuan penelitian ini adalah untuk mengetahui efektifitas bubuk rumput laut merah (Gracillaria sp) sebagai imunostimulan infeksi bakteri Streptococcus iniae pada ikan lele dumbo (Clarias gariepinus). Metode yang digunakan pada penelitian ini yaitu secara eksperimental dengan rancangan acak lengkap (RAL) non faktorial dengan empat perlakuan tiga kali ulangan. Perlakuan adalah pemberian bubuk rumput laut merah secara perendaman dengan dosis perlakuan A (125 mg/l), perlakuan B (100 mg/l), perlakuan C (75 mg/l) dan perlakuan D (kontrol) tanpa perendaman dengan bubuk rumput laut merah. Hasil penelitian menunjukkan perendendaman bubuk rumput laut merah terhadap ikan lele dumbo dengan dosis yang berbeda berpengaruh terhadap pencegahan infeksi bakteri Streptococcus iniae pada ikan lele dumbo. Pemberian bubuk rumput laut merah untuk meningkatkan sistem imun ikan lele dumbo menunjukkan pengaruh sangat nyata terhadap kelangsungan hidup, dimana F hitung (45.111) > F tabel 0,01 (7.59). Perlakuan terbaik terdapat pada perendaman dengan bubuk rumput laut merah dengan dosis 100 mg/l dengan persentase kelangsungan hidup sebesar 83,33%. Selanjutnya kelangsungan hidup terendah terdapat pada perlakuan D (kontrol) tanpa perendaman dengan bubuk rumput laut merah dengan persentase kelangsungan hidup 23,33 % dan mengalami gejala klinis yang paling buruk. Total leukosit pada ikan lele dumbo meningkat pada saat diberi perlakuan perendaman dengan bubuk rumput laut merah dan pada saat uji tantang dengan bakteri Streptococcus iniae. Kualitas air selama penelitian berada pada kondisi yang optimal untuk pertumbuhan ikan lele dumbo dengan kisaran Do 5,0-6,4 ppm, suhu 28-30 0C dan pH 7,0-7,3.Catfish farming has a potential economic value for its commodity in export trading. This study was conducted in May until June 2015 at Physiology Laboratory and Aquatic Laboratory 0f Veteranary Faculty, Syiah Kuala University, then at Laboratory of BBI Ujung Batee. Aceh Besar. The purpose of this study was to determine the effectiveness of red seaweed powder (Gracillaria sp) as immunostimulant againts Streptococcus iniae bacterial infection in catfish (Clarias gariepinus). The method used in this study was experimental method by using a completely randomized design (CRD) non factorial with four treatments of three replications. The factor of experiment was different dosages of red seaweed powder throld soaking treatment. Those different dosages were A (125 mg / l), treatment B (100 mg / l), treatment C (75 mg / l) and treatment D (control) without the red seaweed powder. The results showed that red seaweed powder word give prevention on bacterial infection in catfish. Aplication of red seaweed powder to the immune system of catfish showed highly significant effect on survival rate where F count (45,111)> F table 0,01 (7,59). The hignest survival rate was obtainied from red seaweed powder at a dose of 100 mg/l with a survival rate 83.33%. Furthermore, the lowest one was from the treatment D (control) without soaking by red seaweed powder which survival rate was 23.33% then its clinical symptoms were the worst one. Total leukocytes of catfish inereased when the fish was red seawed powder treated and bacterial challenge test. Water quality during the study was in optimal conditions for the growth of the catfish which the parameters water DO 5.0 to 6.4 ppm, a temperature 28-30 0C and pH 7.0 to 7.3.

scholarly journals Alginate oligosaccharide/polysaccharide and lactic acid bacteria (Lactobacillus bulgaricus FNCC–0041 & Streptococcus thermophilus FNCC–0040) as immunostimulants against pathogenic Vibrio spp. using Artemia bio model

2021 ◽  
Vol 919 (1) ◽  
pp. 012060
Author(s):  
Ervia Yudiati ◽  
D P Wijayanti ◽  
N Azhar ◽  
A I Chairunnisa ◽  
S Sedjati ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Challenge Test ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Bulgaricus ◽  
Pathogenic Vibrio ◽  
Two Factors ◽  
Alginate Oligosaccharide ◽  
Positive Effect

Abstract Alginate polysaccharide/oligosaccharide (APS/AOS) has been proven as a good immunostimulant. FNCC–0041 & FNCC–0040 are lactic acid bacteria (LAB) producing exopolysaccharides. Vibrio bacteria are pathogenic for aquatic cultivans. This study determines the performance of APS/AOS in combination with LAB as immunostimulants. These were conducted in Artemia challenge test assay against three species of Vibrio spp., namely Vibrio parahaemolyticus (Vp), V. harveyi (Vh), and V. vulnificus (Vv). The treatments were prepared by Factorial Design with two factors (APS/AOS doses and Vibrio spp.) and replicated three times. The APS/AOS concentration was 0, 300, 600, 900 ppm. There were 8 levels of vibrio challenges, namely non-Vibrio, Vp, Vh, Vv, Vp-Vh, Vh-Vp, Vv-Vp, and Vp-Vh-Vv. LAB and APS/AOS were fermented with seawater encapsulated by newly hatched Artemia’s nauplii for one hour. Ten nauplii were taken out and challenged with 108 cells/mL Vibrio. Its survival rate (SR) was counted every six hours until reached 100% mortality. Results show that SR of all nauplii Artemia bio encapsulated treatments was higher than control (p<0.05). The best survival rate was reached from 400 ppm AOS. It has appeared that there is a synergically positive effect among the bio encapsulated AOS and LAB to accelerate the Artemia’s immune system.

scholarly journals Selection of lactic acid bacteria as a probiotic and evaluated its performance on gnotobiotic catfish Clarias sp.

2018 ◽  
Vol 17 (1) ◽  
pp. 68
Author(s):  
Enita Romasni Turnip ◽  
Widanarni, Widanarni ◽  
Anja Meryandini
Keyword(s):  
Growth Rate ◽  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Challenge Test ◽  
Feed Conversion

<p class="JudulBabdenganNomor">ABSTRACT</p><p class="JudulBabdenganNomor"> </p><p>This study aimed to select lactic acid bacteria (LAB) as a potential probiotic that producing anti‒microbial compounds in order to treat motile aeromonads septicemia diseases caused by <em>Aeromonas hydrophila</em> on catfish <em>Clarias</em> sp. and evaluated its performance on gnotobiotic catfish. The <em>in vitro</em> assay was done to select several LAB isolates based on antagonistic activity against pathogenic bacteria. The selected isolate was tested <em>in vivo</em> to observe their ability to improve growth performances of catfish. The study was conducted with five treatments consists of K‒ (normal catfish without addition probiotic, without challenge test), K+ (normal catfish without addition of probiotic, with challenge test), Np (normal catfish with addition of probiotic and challenge test), G (gnoto catfish without addition of probiotic, with challenge test), and Gp (gnoto catfish with addition of probiotic and challenge test). The results showed that the addition of <em>Pediococcus pentosaceus</em> E2211 as selected probiotic could increase survival rate, specific growth rate, and immune response towards infection of <em>A. hydrophila</em>. The best survival rate after challenge test was obtained in Np and Gp treatments (88.46%), followed by G treatment (65.38%), while the K+ was only 53.84%. The conclusion of this study was <em>P. pentosaceus</em> E2211 potentially used as a probiotic candidate for normal and gnotobiotic catfish. The presence of normal microflora with <em>P. pentosaceus</em> E2211 in Np treatment showed the best probiotic performance with daily growth rate 3.28%, feed conversion ratio 1.79, and total intestinal bacteria reached 10<sup>8</sup> CFU/mL significantly different from other treatments (P&lt;0.05).</p><p>Keywords: <em>Aeromonas hydrophila,</em> catfish, LAB, probiotic, screening</p><p> </p><p> </p><p align="center"><strong>ABSTRAK</strong></p><p>Tujuan penelitian ini adalah menyeleksi bakteri asam laktat (BAL) sebagai probiotik potensial penghasil senyawa antimikrob guna menanggulangi penyakit <em>motile aeromonad septicemia</em> akibat <em>Aeromonas hydrophila</em> pada ikan lele <em>Clarias </em>sp. dan evaluasi kinerjanya pada ikan lele gnotobiotik. Pengujian <em>in vitro </em>dilakukan untuk menyeleksi beberapa isolat BAL sebagai kandidat probiotik berdasarkan aktivitas antagonis terhadap bakteri patogen. Isolat terpilih kemudian diuji <em>in vivo</em> untuk mengetahui kemampuannya dalam meningkatkan performa tumbuh ikan lele.<em> </em>Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan, yaitu: K‒ (lele normal tanpa probiotik dan tanpa tanpa diuji tantang), K+ (lele normal tanpa probiotik dan diuji tantang), Np (lele normal diberi probiotik dan diuji tantang), G (lele gnoto tanpa probiotik dan diuji tantang), dan Gp (lele gnoto diberi probiotik dan diuji tantang). Hasil penelitian menunjukkan pemberian probiotik terpilih BAL <em>Pediococcus</em><em> pentosaceus</em> E2211 mampu meningkatkan sintasan, laju pertumbuhan, dan respons imun ikan lele terhadap infeksi <em>A. hydrophila</em>. Sintasan terbaik pascauji tantang diperoleh pada perlakuan Np dan Gp yaitu sebesar 88,46%, diikuti perlakuan G sebesar 65,38%, sementara pada K+ hanya mencapai 53,84%. Kesimpulan dari penelitian ini ialah isolat BAL terpilih <em>P. pentosaceus </em>E2211 berpotensi sebagai kandidat probiotik untuk ikan lele normal dan lele gnotobiotik <em>Clarias </em>sp. Keberadaan mikroflora normal yang berasosiasi dengan <em>P. pentosaceus </em>E2211 pada perlakuan Np menunjukkan kinerja probiotik terbaik dengan nilai laju pertubuhan harian 3,28%, rasio konversi pakan 1,79 dan total bakteri usus mencapai 10<sup>8</sup> CFU/mL yang berbeda signifikan dibanding perlakuan lainnya (P&lt;0,05).</p><p>Kata kunci: <em>A. hydrophila,</em> BAL, ikan lele, probiotik, seleksi</p><p> </p>

scholarly journals The Effect of Adding Iodine to the Feed to Increase Growth and Survival Rate of Carp Seeds (Cyprinus carpio)

2020 ◽  
Vol 20 (2) ◽  
pp. 314
Author(s):  
Ayu Nirmaniar Fadliani ◽  
Ayu Adhita Damayanti ◽  
Ibadur Rahman
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Iodine Content ◽  
The Body ◽  
Feed Conversion ◽  
Feed Supplementation ◽  
Growth And Survival ◽  
Randomized Design ◽  
Completely Randomized Design

One of the factors that greatly influence the successful growth of cultivated fish is feed. The aim of this study was to examine the effect of iodine addition on the growth and survival rate of carp (Cyprinus carpio) seeds. This study used a completely randomized design (CRD) method with 4 treatments and 3 replications. 10 fish per unit each, with treatment P0 (feed without adding iodine), P1 (feed + iodine at a dose of 10 mg / kg), P2 (feed + iodine at a dose of 20 mg / kg), P3 (feed + iodine at a dose of 30 mg / kg). The results showed that there was no significant effect (p> 0.05) on the growth rate, feed conversion and survival rate of goldfish. So it can be concluded that the addition of iodine in feed supplementation up to a dose of 30 mg / kg does not have a significant effect on the growth rate of length and absolute weight, feed conversion, survival rate and iodine content in the body of goldfish (Cyprinus carpio). The best recommended dose of iodine for growth is 20 mg / kg of feed, although the results were not significantly different for growth. However, it can have an effect on the iodine content in the body of goldfish.

scholarly journals Effects of Feed Mixed with Lactic Acid Bacteria and Carbon, Nitrogen, Phosphorus Supplied to the Water on the Growth and Survival Rate of White Leg Shrimp (Penaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Caused by Vibrio parahaemolyticus

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 280
Author(s):  
Linh Nguyen Thi Truc ◽  
Tuu Nguyen Thanh ◽  
To Tran Thi Hong ◽  
Day Pham Van ◽  
Minh Vo Thi Tuyet ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Vibrio Parahaemolyticus ◽  
Control Group ◽  
Penaeus Vannamei ◽  
Growth And Survival ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Nitrogen Phosphorus ◽  
Shrimp Health

This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum, have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly (p < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of V. parahaemolyticus effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30–50% in the period after three days of the challenge, except in L. plantarum treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp’s immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.

scholarly journals Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chatchai Kaewpila ◽  
Pongsatorn Gunun ◽  
Piyawit Kesorn ◽  
Sayan Subepang ◽  
Suwit Thip-uten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Acid Fermentation ◽  
Design Factor ◽  
Forage Sorghum ◽  
Randomized Design

AbstractImproving the nutrition of livestock is an important aspect of global food production sustainability. This study verified whether lactic acid bacteria (LAB) inoculant could promote ensiling characteristics, nutritive value, and in vitro enteric methane (CH4) mitigation of forage sorghum (FS) mixture silage in attacking malnutrition in Zebu beef cattle. The FS at the soft dough stage, Cavalcade hay (CH), and cassava chip (CC) were obtained. The treatments were designed as a 4 × 2 factorial arrangement in a completely randomized design. Factor A was FS prepared without or with CH, CC, and CH + CC. Factor B was untreated or treated with Lactobacillus casei TH14. The results showed that all FS mixture silages preserved well with lower pH values below 4.0 and higher lactic acid contents above 56.4 g/kg dry matter (DM). Adding LAB boosted the lactic acid content of silages. After 24 h and 48 h of in vitro rumen incubation, the CC-treated silage increased in vitro DM digestibility (IVDMD) with increased total gas production and CH4 production. The LAB-treated silage increased IVDMD but decreased CH4 production. Thus, the addition of L. casei TH14 inoculant could improve lactic acid fermentation, in vitro digestibility, and CH4 mitigation in the FS mixture silages.

scholarly journals The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

2020 ◽  
Vol 6 (1) ◽  
pp. 88-95
Author(s):  
Marlina Doloksaribu ◽  
Robert Martua Simanjuntak ◽  
Ied Hidayani Parinduri
Keyword(s):  
Social Sciences ◽  
Experimental Study ◽  
Data Analysis ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Fish Farming ◽  
Randomized Design ◽  
The Social ◽  
Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

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