scholarly journals Efektifitas bubuk rumput laut merah (Gracillaria sp) sebagai imunostimulan terhadap infeksi bakteri streptococcus iniae pada ikan lele dumbo (Clarias gariepinus)

2016 ◽  
Vol 3 (2) ◽  
pp. 81
Author(s):  
Cut Sofia Amanda ◽  
Eva Ayuzar

Usaha budidaya ikan lele dumbo memiliki nilai ekonomis yang tinggi karena merupakan salah satu komoditas hasil perikanan yang potensial untuk diekspor. Penelitian ini dilaksanakan pada Bulan Mei hingga Bulan Juni 2015 dan dilaksanakan di Laboratorium Fisiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Laboratorium Akuatik Fakultas Kedokteran Hewan Universitas Syiah Kuala dan laboratorium Mikrobiologi BBI Ujong Batee Aceh Besar. Tujuan penelitian ini adalah untuk mengetahui efektifitas bubuk rumput laut merah (Gracillaria sp) sebagai imunostimulan infeksi bakteri Streptococcus iniae pada ikan lele dumbo (Clarias gariepinus). Metode yang digunakan pada penelitian ini yaitu secara eksperimental dengan rancangan acak lengkap (RAL) non faktorial dengan empat perlakuan tiga kali ulangan. Perlakuan adalah pemberian bubuk rumput laut merah secara perendaman dengan dosis perlakuan A (125 mg/l), perlakuan B (100 mg/l), perlakuan C (75 mg/l) dan perlakuan D (kontrol) tanpa perendaman dengan bubuk rumput laut merah. Hasil penelitian menunjukkan perendendaman bubuk rumput laut merah terhadap ikan lele dumbo dengan dosis yang berbeda berpengaruh terhadap pencegahan infeksi bakteri Streptococcus iniae pada ikan lele dumbo. Pemberian bubuk rumput laut merah untuk meningkatkan sistem imun ikan lele dumbo menunjukkan pengaruh sangat nyata terhadap kelangsungan hidup, dimana F hitung (45.111) > F tabel 0,01 (7.59). Perlakuan terbaik terdapat pada perendaman dengan bubuk rumput laut merah dengan dosis 100 mg/l dengan persentase kelangsungan hidup sebesar 83,33%. Selanjutnya kelangsungan hidup terendah terdapat pada perlakuan D (kontrol) tanpa perendaman dengan bubuk rumput laut merah dengan persentase kelangsungan hidup 23,33 % dan mengalami gejala klinis yang paling buruk. Total leukosit pada ikan lele dumbo meningkat pada saat diberi perlakuan perendaman dengan bubuk rumput laut merah dan pada saat uji tantang dengan bakteri Streptococcus iniae. Kualitas air selama penelitian berada pada kondisi yang optimal untuk pertumbuhan ikan lele dumbo dengan kisaran Do 5,0-6,4 ppm, suhu 28-30 0C dan pH 7,0-7,3.Catfish farming has a potential economic value for its commodity in export trading. This study was conducted in May until June 2015 at Physiology Laboratory and Aquatic Laboratory 0f Veteranary Faculty, Syiah Kuala University, then at Laboratory of BBI Ujung Batee. Aceh Besar. The purpose of this study was to determine the effectiveness of red seaweed powder (Gracillaria sp) as immunostimulant againts Streptococcus iniae bacterial infection in catfish (Clarias gariepinus). The method used in this study was experimental method by using a completely randomized design (CRD) non factorial with four treatments of three replications. The factor of experiment was different dosages of red seaweed powder throld soaking treatment. Those different dosages were A (125 mg / l), treatment B (100 mg / l), treatment C (75 mg / l) and treatment D (control) without the red seaweed powder. The results showed that red seaweed powder word give prevention on bacterial infection in catfish. Aplication of red seaweed powder to the immune system of catfish showed highly significant effect on survival rate where F count (45,111)> F table 0,01 (7,59). The hignest survival rate was obtainied from red seaweed powder at a dose of 100 mg/l with a survival rate 83.33%. Furthermore, the lowest one was from the treatment D (control) without soaking by red seaweed powder which survival rate was 23.33% then its clinical symptoms were the worst one. Total leukocytes of catfish inereased when the fish was red seawed powder treated and bacterial challenge test. Water quality during the study was in optimal conditions for the growth of the catfish which the parameters water DO 5.0 to 6.4 ppm, a temperature 28-30 0C and pH 7.0 to 7.3.

2020 ◽  
Vol 5 (1) ◽  
pp. 138-152
Author(s):  
Rosidah Rosidah ◽  
Yuniar Mulyani ◽  
Walim Lili ◽  
Khasanatur Rosyidah

Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of  leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9%  and the highest survival rate was 80%. 


2017 ◽  
Vol 11 (3) ◽  
pp. 249
Author(s):  
Lisa Fajar Indriana ◽  
Yuli Afrianti ◽  
Sitti Hilyana ◽  
Muhammad Firdaus Firdaus

Teripang pasir, Holothuria scabra merupakan komoditas hasil laut yang bernilai ekonomis tinggi. Penangkapan berlebihan stok di alam mendorong berkembangnya kegiatan budidaya. Penempelan merupakan fase kritis pada larva teripang karena terjadi peralihan sifat planktonis ke bentik yang memerlukan substrat. Penelitian ini bertujuan untuk mengetahui preferensi penempelan, pertumbuhan, dan sintasan larva H. scabra fase penempelan pada substrat lamun yang berbeda. Penelitian menggunakan metode rancangan acak lengkap dengan perlakuan empat jenis daun lamun berbeda dan lima ulangan. Perlakuan terdiri atas Enhalus acoroides (L-1), Syringodium isoetifolium (L-2), Cymodocea serrulata (L-3), dan Cymodocea rotundata (L-4). Jumlah awal larva sebanyak 1.000 individu dan substrat dirangkai dengan luasan yang sama sebesar 12 cm x 17 cm untuk setiap unit penelitian. Hasil penelitian menunjukkan jenis lamun yang digunakan sebagai substrat berpengaruh secara nyata terhadap preferensi penempelan dan sintasan larva teripang pasir, namun tidak berpengaruh terhadap pertumbuhan. E. acoroides menunjukkan hasil terbaik dengan preferensi penempelan 0,26 ind. cm-2 dan sintasan 10,66%; sehingga layak digunakan sebagai substrat penempelan dalam pembenihan teripang pasir, H. scabra.Holothuria scabra larvae on different seagrass substrates. By: Lisa Fajar Indriana, Yuli Afrianti, Sitti Hilyana, and Muhammad FirdausSandfish Holothuria scabra is marine commodities with a high economic value. Overfishing of natural stocks has compelled an interest to begin aquaculture practice. Settlement is a critical phase for the planktonic larvae as they will transform to benthic form in the presence of substrate. This study aims to evaluate the settlement preferences, growth, and survival rate of H. scabra larvae settled on different seagrass leaves. The research was conducted using the Completely randomized design with four different species of seagrass leaves and five replications. The treatments consist of Enhalus acoroides (L-1), Syringodium isoetifolium (L-2), Cymodocea serrulata (L-3), and Cymodocea rotundata (L-4). Initial number of larvae was 1,000 individuals and the substrate was set with same widthof 12 cm x 17 cm for each unit. Results of the experiment indicated that settlement preference and survival rate of H. scabra larvae was significantly affected by seagrass used as substrate while no significantly differences was observed for growth of larvae. E. acoroides showed the best result with 0.26 ind. cm-2 settelement preference and 10.66% survival rate, so that suitable to be used as settlement substrate in H. scabra hatchery.


2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae


2020 ◽  
Vol 9 (2) ◽  
pp. 86
Author(s):  
Oktaviana Elsa Dewi ◽  
Sapto Andriyono ◽  
Mochammad Amin Alamsjah

Aquaponics is a technology that combines the cultivation of vegetable production and fish farming. The aquaponics technology also to fertilization to give nutrients to the plant to support of survival. In facts, fertilization done to plants is located on land. Fertilizing the plants that live in the waters are still very rare, because the waters as a growing medium that is considered to always provide sufficient nutrients for plant growth. In this research, using biofertilizer Euchema cottonii of seaweed. The advantages of biofertilizer seaweed is to have the content of macro and micro nutrients are complete. It also contains substances hyper Grow (PGR) plant (auxin, cytokinin, gibberellin, abisat acid and ethylene).In this research to determine the effect of biofertilizer products of seaweed (E. cottonii) commercial on the growth of dumbo catfish (C. gariepinus) and pakcoy mustard (B. rapa L) on aquaponics system. The design of experiments in this research using a completely randomized design (CRD) with 5 treatments and 4 replications. The treatment used is fertilizer "AB mix®" 1% (A), without fertilizer (B) and the provision of products biofertilizer seaweed (E. cottonii) commercial 0,16% (C), 0,18% (D) and 0,20% (E). The main parameters measured were heavy growth of biomass, plant height pakcoy mustard (B. rapa L), growth SGR, length and survival rate of fish of dumbo catfish (C. gariepinus).The results showed that the dose of different commercial product biofertilizer seaweed (E. cottonii) gives a significantly different effect on the height growth of pakcoy mustard (B. rapa L) and the survival rate of dumbo catfish (C. gariepinus). The optimal dosage of commercial seaweed (E. cottonii) biofertilizer product in increasing the growth of pakcoy mustard (B. rapa L.) and African catfish (C. gariepinus) in the aquaponic system, namely treatment E at a dose of 0.20%.


2020 ◽  
Vol 6 (1) ◽  
pp. 72-80
Author(s):  
Helentina Mariance Manullang

This study aimed to determine the effect of the use of EM-4 probiotics (effective microorganisms-4) in feed on different stockings in increasing the survival rate and growth of African catfish. This research was conducted by the Faculty of Fisheries Laboratory of the Dharmawangsa University Medan from September to October 2019. The design used in this study was a completely randomized design, with 4 treatments 3 replications. The treatment consists of the different amount of stocking density (P1: 50 fish / 48 liters of water), (P2: 65 fish / 48 liters of water), (P3: 80 fish / 48 liters of water) and (P4: 95 fish / 48 liters of water). The observation result Data is analyzed by variance analysis (ANAVA) after meeting the requirements, Subsequently conducted a variety of error test homogenized and use a chi-squared spread with the formula Steel and Torries (2003). The results showed that the different amount of stocking density affected the growth and graduation of African catfish (Clarias gariepinus) seeds. The optimal density of stocking on the growth and graduation of live African catfish is the density of 50 fish/ 48 liters of water, with an average survival rate of 90.66%, the average growth of absolute weight  19.10 grams and average average growth length 9 cm


2014 ◽  
Vol 1 (1) ◽  
pp. 14
Author(s):  
Muhammad Robi ◽  
Erlangga Erlangga

Kepiting bakau (Scylla serrata) merupakan jenis golongan Crustaceae   yang mengandung protein hewani yang cukup tinggi dan memiliki nilai ekonomis yang tinggi. Ablasi mata merupakan salah satu cara untuk menghilangkan hambatan perkembangan telur (gonad) pada kepiting bakau. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) non factorial dengan 4 perlakuan dan 3 ulangan. Adapun perlakuan dalam penelitian ini adalah perlakuan A (ablasi satu pasang mata), perlakuan B (ablasi mata kiri), C (ablasi mata kanan), D (tanpa ablasi).   Hasil penelitian menunjukkan bahwa kecepatan kematangan gonad tertinggi pada perlakuan C (ablasi mata kanan) yaitu pertumbuhan gonad mencapai 21,53% dan terendah terjadi  D (tanpa ablasi)  pertumbuhan gonad hanya 14,8%. Analisa statistik dengan uji F menunjukan bahwa ablasi mata berpengaruh terhadap kecepatan kematangan gonad kepiting bakau. Pertambahan bobot tertinggi  terdapat pada perlakuan C (ablasi mata kanan) yaitu sebesar 2,67 gr kemudian disusul oleh D (tanpa ablasi)  sebesar 1,89 gr  dan terakhir  pada B (ablasi mata kiri) dengan nilai rata-rata sebesar 0,77 gr. Tingkat kelangsungan hidup terbaik terdapat pada perlakuan D (tanpa ablasi)   yaitu 100%, perlakuan B (ablasi mata kiri) 77,77%, perlakuan C (ablasi mata kanan) 66,66% dan kelansungan hidup terendah terjadi pada perlakuan A (ablasi satu pasang mata) 0%. Rata-rata kualitas air selama penelitian adalah suhu 27 0C dan Ph 7,65.Mangrove crab (Scylla serrata) have been classifying in Crustaceae clas that containing high animal protein and high economic value. Ablation of the eye is an solution to eliminate the egg development (gonads) barriers in the mangrove crab. The research design used a completely randomized design (CRD) non- factorial with 4 treatments and 3 replications. Treatment in this study were the treatment of A (ablation of the pairs of eyes), treatment B (left eye ablation), treatment C (right eye ablation), D (without ablation). The results showed that the highest rate of gonadal maturation was in treatment C (right eye ablation) where gonadal development growth reached 21.53 %, whereas the lowest was in treatment D (without ablation) where gonadal development growth was only 14.8 %. Statistical analysis by F test showed that ablation of the eye was affected the mud crab gonadal maturity. The Weight gain was found highest in the treatment C (right eye ablation) that reached 2.67 g and followed by treatment D (without ablation) 1.89 grams and the last in the treatment B (left eye ablation) with was an average value 0.77 grams. The higest survival rate was found in treatment D (without ablation) 100 %, treatment B (left eye ablation) 77.77 %, treatment C (right eye ablation) 66.66 % and the lowest survival rate was occurred in treatment A (ablation of the pairs of eyes) 0 %. The avarge values of water quality during the study showed that the temperature was reched 27 0C and pH was 7, 65.


2020 ◽  
Vol 12 (1) ◽  
pp. 113
Author(s):  
Diana Maulianawati ◽  
Rukisah Rukisah ◽  
Awaludin Awaludin ◽  
Muhammad Iswan Guntur

HighlightsThe Paku Uban (Nephrolepis biserrata) was identified and analyzedThe ability of paku uban to stimulated molting process of Scylla sp was analyzedThe paku uban (Nephrolepis biserrata) could increase the presentation of molting and accelerate molting processAbstractMud crab (Scylla spp.) is a fishery commodity with high economic value. For this reason, efforts to increase production are required with a more effective applicative technology. This study aims to determine the molting response and survival rate of mud crabs injected with paku uban leaf  extract (N. biserrata) at different doses. The study was conducted for 30 days, located in Tibi Island, Tanah Lia District, Tanah Tidung. The design used in this study was a completely randomized design (CRD) with five treatments and eight replicates. Mud crabs weighing 80 - 150 g were tested in K1 (without injection), K2 (controlled injection), P1 (100 ppm extract), P2 (125 ppm extract) and P3 (150 ppm extract). The effectivity of paku uban (N. biserrata) extract with the highest percentage of molting of 50% took place at P3, 37.5% at P2, and 25% at P1. The lowest levels of molting percentage were at K1 and K2 by 0%. The fastest molting period was ten days at P2, and the longest was 29 days at P1. The highest weight growth reached 33.75 g at P3, followed by P2 and P1 with 31 and 18.75 g, respectively. The survival rate of mud crabs for all treatments reached 100%. The results of the analysis of variance indicated a significant bet effect (P < 0.05) on the molting and weight growth percentage. The BNT test suggested that a dose of 150 ppm was the optimum treatment. 


2019 ◽  
Vol 2 (2) ◽  
pp. 78
Author(s):  
Ahmad Khabib Ulin Nuha ◽  
Andi Rahmad Rahim ◽  
Aminin Aminin

As one of fish commodity, Catfish (Pangasius pangasius) has a very important economic value for the community. The time needed for catfish cultivation tends to be long and the price of feed is high so that it triggers the less optimal production of catfish. One way to maximize good production and with minimized time is by adding multivitamin probiotics to the feed. This research aims to analyze the optimal dose of probiotics to the growth and survival rate of catfish . Method used is Complete Randomized Design Design (CRDD) using 4 treatments of probiotic doses and 3 replicates. The treatments are A Control (0.00 ml/kg), B (10 ml/kg), C (15 ml/kg), and D (20 ml/kg). Absolute weight, absolute length, and survival of catfish are the main variables observed. The data were analyzed by using the analysis of variance and continued with the LSD test (the Least Significant Difference), Ü = 0.05. The optimal dose of probiotics based on the absolute length and absolute weight of catfish is 10 ml/kg and it produces an absolute weight of 5.27 gram and absolute length of 5.76 cm. The optimal daily specific growth rate is at the probiotics dose of 10 ml/kg which is 2.302.55%/day. The highest survival rate for catfish is 100% at probiotic doses of 0.000 ml/kg, 10 ml/kg and 15 ml/kg. The range of water quality is in the temperature of 27.5 – 28,90C, pH 6.79 - 7.88, DO 6.4 - 7.9 ppm, and ammonia 0 ppm.


2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Christo V.S Aer ◽  
Winda M Mingkid ◽  
Ockstan J Kalesaran

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.


2018 ◽  
Vol 1 (2) ◽  
pp. 22
Author(s):  
Setiawan Eka Putra ◽  
Endah Sri Redjeki ◽  
Sa’idah Luthfiyah

African catfish (Clarias gariepinus) is one type of freshwater fish that has high economic value. African catfish with intensive cultivation are hampered by the high cost of feed that is not in accordance with the selling price of African catfish. An alternative effort that can be done is the addition of probiotics to feed which is expected to reduce feed costs. Probiotics are living microorganisms in fish culture that can prevent disease, thereby increasing production and can reduce economic losses. This study aims to analyze the effect of probiotics on the growth of absolute weight and daily growth rate of African catfish. The method used was Completely Randomized Design (RAL) with 4 treatments namely 0, 2, 4 and 6 ml / kg of feed and repeated 3 times. Activities carried out in this study include preparation of containers, seed stocking, fish sampling, feed preparation, feeding and water management. The variables observed were absolute weight growth and daily growth rate. The results showed that the addition of probiotics to commercial feed significantly affected the growth of weight and daily growth rate of African catfish


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